LOCUS       MR231681                8502 bp    DNA     circular  SYN 
DEFINITION  Tyk2 (myc-DDK-tagged) - Mouse tyrosine kinase 2 (Tyk2), transcript variant 1
ACCESSION   NM_001205312
VERSION     NM_001205312.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4659..4750 
                     /label="Myc-DDK"  
     misc_feature    4780..5365 
                     /label="PolyA signal"  
     misc_feature    4837..4856 
                     /label="Restriction Enzyme"  
     misc_feature    5641..6313 
                     /label="ColE1"  
     misc_feature    6743..7537 
                     /label="Kan/Neo"  
     misc_feature    7572..7874 
                     /label="SV40 ori"  
     misc_feature    7960..8437 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4641..4647 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4641 
                     /label="Tyk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGGGACC ATGCCTCTGT GTGGGCGGAG AGCCATCTTG GAAGACAGCA 
     1081 AGGCAGATGG CACAGAAGCT CAGCCCCTGG TGCCCACAGG ATGCTTGATG GTGCTACTGC 
     1141 ATTGGCCTGG GCCTGAGGGC GGGGAGCCCT GGGTCACCTT CAGCCAGACA TCTCTGACTG 
     1201 CAGAGGAGGT CTGCATCCAC ATCGCACACA AAGTCGGCAT CACTCCACCC TGCTTGAATC 
     1261 TCTTCGCACT CTACAATGCA CAGGCTAAGG TCTGGCTGCC CCCAAACCAT ATTCTGGATA 
     1321 CATCCCAAGA CATGAACCTC TATTTTCGAA TGAGGTTTTA CTTCCGGAAC TGGCATGGCA 
     1381 TGAATCCCCA GGAGCCAGCT GTATACCGGT GTGGTTTCCC AGGGGCAGAG ACTTCCTCAG 
     1441 ACCGGGCAGA GCAAGGTGTA CAGCTCTTGG ACTCTGCCTC GTTTGAATAC CTTTTTGAGC 
     1501 AGGGATGGTA TGTTGGCAAT AGAGCAAAAA GCTCCCACAC CTCTTTTTCT ATCCATCGCT 
     1561 CTACCCCTCA GGGAAAGCAT GAGTTCATGA ACGATGTGGT GTCTCTGCGG GACCTGTCTA 
     1621 GCGAGGAGGA GATCCACCAC TTTAAGAATG AGAGCTTAGG CATGGCCTTC CTGCACCTGT 
     1681 GTCACCTTGC TCTCAGCCGA GGCGTCCCCC TGGAGGAGAT GGCCAGAGAG ATCAGCTTCA 
     1741 AGAACTGCAT CCCTCATTCC TTCCGACAGC ACATCCGGCA GCACAATGTG CTCACACGCC 
     1801 TGCGGCTCCA CAGAGTCTTC CGCCGCTTCC TGCGGGCCTT CCGGCCTGGC CACCTCTCCC 
     1861 AGCAGGTTGT GATGGTGAAG TACTTGGCTA CCCTGGAGCG GCTGGCTCCG CGCTTTGGCT 
     1921 CAGAGCGCAT ACCTGTGTGT CATCTGGAGG TCCTGGCACA GCCCGAGAGG GACCCCTGCT 
     1981 ACATCCAGAA CAGTGGGCAG ACCGCTGGGG ATCCAGGCCC AGAGCTGCCT TCCGGGCCTC 
     2041 CCACCCACGA GGTACTGGTG ACAGGCACCG GAGGTATCCA GTGGCATCCG CTGCAGACCC 
     2101 AGGAATCTGA GAGAGGTAAC AGCAGAGGGA ATCCCCACGG CAGCCGGTCG GGGAAGAAGC 
     2161 CCAAAGCCCC CAAGGCTGGA GAGCACCTGA CAGAGAGCCC TCAGGAACCA CCTTGGACCT 
     2221 ACTTCTGTGA CTTCCAGGAC ATTTCCCACG TGGTGCTAAA GGAGCGTCGC GTGCACATCC 
     2281 ACCTCCAAGA CAACAAGTGC TTGTTGCTGT GCCTCTGTTC CCAGGCTGAG GCCCTGTCCT 
     2341 TTGTGGCCCT GGTCGATGGC TATTTCCGCT TGACTGCTGA CTCCAGCCAC TATCTGTGCC 
     2401 ATGAGGTGGC ACCCCCGAGG CTGGTGACTA GCATCCAGAA CGGCATCCAT GGGCCCCTGA 
     2461 TGGATCCGTT TGTACAGGCC AAGCTGTGGC CAGAGGACGG CCTCTACCTG ATTCAGTGGA 
     2521 GCACCAGCCA CCTGCACCGC CTGATCCTTA CCGTGGCCCA TCGAAACCCG GCTTTCAGTA 
     2581 ATGGCCCTCG GGGCCTGCGC CTGCGAAAGT TCCCCATCAC ACAGCAGCCT GGAGCCTTTG 
     2641 TGCTGGATGG CTGGGGCCGC TCCTTTGCCA GCTTGGGGGA CCTTCGGCTC GCCTTGCAGG 
     2701 GCTGCTCGTT GCGGGCCGGT GATGACTGTT TCCCCCTGCA CCACTGCTGC CTGCCCCGGC 
     2761 CAAGAGAAAT CTCCAACCTC GTCATCATGC GGGGGTCTAG GGCCCACACC CGGCCTCTCA 
     2821 ACCTCAGTCA GCTCAGCTTC CACAGGGTTC ACCAGGATGA AATCACCCAG CTGTCCCACT 
     2881 TGGGCCAAGG CACAAGGACC AATGTGTATG AGGGCCTTCT AAGAGTGGGA GGCCCCGATG 
     2941 AGGGCAAAGT GGACAATGGA TGTCCCCCTG AGCCTGGTGG GACTAGTGGG CAGCAGCTTC 
     3001 GAGTGGTGCT CAAAGTTCTC GACCCCAGTC ACCATGATAT CGCCTTGGCC TTCTATGAGA 
     3061 CTGCTAGCCT CATGAGCCAG GTGTCACACA TGCACCTGGC TTTCCTGCAT GGTGTTTGCG 
     3121 TGCGTGGCTC AGAGAATATC ATTGTGACAG AATTCGTAGA ACATGGTCCC TTGGATGTGT 
     3181 GGTTACGGCG ACAGAGGGGC CAAGTGCCCA TGACCTGGAA GATGGTCGTG GCTCAGCAGC 
     3241 TGGCCAGCGC CCTCAGCTAC CTGGAGGACA AGAATCTGGT TCACGGGAAT GTATGTGGCC 
     3301 GGAACATCCT GCTGGCTCGC CTGGGGTTGG AGGAGGGTAC CAACCCCTTC ATCAAGCTAA 
     3361 GTGATCCTGG TGTGGGCCAG GGTGCCCTCT CCAGGGAAGA GCGGGTGGAG CGCATCCCCT 
     3421 GGACAGCTCC CGAGTGCCTG TCTGGAGGGA CCAGTAGCTT GGGTACTGCC ACGGACATGT 
     3481 GGGGCTTTGG TGCCACCCTT CTTGAGATCT GCTTTGATGG GGAGGCACCC CTGCAGGGTC 
     3541 GTGGTCCCTC TGAGAAAGAA CGGTTCTACA CAAAGAAACA TCAGCTGCCT GAACCCTCAT 
     3601 CCCCAGAGCT GGCCACACTC ACCCGCCAGT GCCTGACCTA TGAACCAGCA CAGCGGCCAT 
     3661 CCTTCCGCAC CATTTTGCGG GACCTCACAA GGCTGCAGCC ACAGAATCTA GTCGGCACTT 
     3721 CGGCTGTGAA CTCAGACTCA CCAGCATCAG ACCCCACTGT TTTCCACAAG CGCTATTTGA 
     3781 AAAAGATCCG GGATTTGGGT GAGGGTCACT TTGGCAAGGT CAGCCTGTAC TGCTACGACC 
     3841 CAACCAATGA CGGCACTGGC GAGATGGTGG CCGTGAAGGC CCTGAAGGAA GGGTGCGGTC 
     3901 CCCAGCTCCG CTCAGGCTGG CAGCGGGAGA TCGAGATCCT GCGGACGCTG TACCACGAAC 
     3961 ATATTGTCAA GTATAAAGGC TGCTGTGAGG ACCAAGGAGA GAAGTCTGTA CAGCTGGTCA 
     4021 TGGAGTACGT CCCTCTGGGC AGCCTCCGAG ACTACCTGCC AAGGCACTGC GTAGGGCTGG 
     4081 CGCAGCTCCT GCTGTTTGCC CAGCAGATCT GCGAGGGCAT GGCCTACCTG CACGCTCAGC 
     4141 ACTACATTCA CCGAGACCTC GCCGCGCGCA ACGTGCTGCT GGACAACGAC AGGCTGGTCA 
     4201 AGATTGGAGA CTTTGGCCTA GCCAAGGCTG TACCTGAAGG CCACGAGTAC TACCGAGTGC 
     4261 GCGAGGACGG GGACAGCCCA GTGTTCTGGT ATGCCCCAGA ATGCCTGAAG GAGTGCAAAT 
     4321 TTTACTATGC ATCTGATGTC TGGTCCTTCG GGGTAACTCT GTATGAGTTG TTGACATACT 
     4381 GTGACTCTAA CCAGAGTCCC CATATGAAAT TCACCGAGCT CATCGGCCAT ACCCAGGGCC 
     4441 AGATGACCGT GCTGAGGCTC ACAGAGCTGC TGGAACGAGG AGAGAGGCTG CCTCGGCCTG 
     4501 ACAGGTGTCC CTGTGAGATC TATCACCTCA TGAAGAACTG CTGGGAGACA GAGGCCTCCT 
     4561 TCCGGCCCAC CTTCCAGAAT CTTGTGCCCA TCCTCCAGAC AGCACAGGAG AAGTACCAAG 
     4621 GTCAGGTGCC TTCCGTGTTC AGCGTGTGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4681 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4741 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4801 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4861 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4921 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4981 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5041 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5101 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5161 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5221 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5281 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5341 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5401 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6301 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6361 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6421 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6481 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6541 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6601 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6661 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6721 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6781 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6841 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6901 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6961 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7021 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7081 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7141 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7201 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7261 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7321 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7381 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7441 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7501 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7561 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7621 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7681 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7741 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7921 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7981 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8041 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8101 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8161 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8221 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8281 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8341 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8401 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8461 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//