LOCUS       MR231672                8469 bp    DNA     circular  SYN 
DEFINITION  Arap1 (myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 1
ACCESSION   NM_027180
VERSION     NM_027180.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4624..4715 
                     /label="Myc-DDK"  
     misc_feature    4745..5330 
                     /label="PolyA signal"  
     misc_feature    4802..4821 
                     /label="Restriction Enzyme"  
     misc_feature    5606..6278 
                     /label="ColE1"  
     misc_feature    6708..7502 
                     /label="Kan/Neo"  
     misc_feature    7537..7839 
                     /label="SV40 ori"  
     misc_feature    7925..8402 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="Arap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAAGGAG GAGCCCCTGC CGAGCCGAGT CCCACGGGCT GTGCGTGTGG 
     1081 CTAGTCTGCT GAGCGAGGGG GAGGAGCTGT CTGGGGATGA TTCGGAAGAT GACGATGACC 
     1141 ATGCCTATGA GGGTATCCCC AATGGTGGGT GGCCAACGAG TGGCCTGAAT CCACCCTTAC 
     1201 GCAGCCTGAT CCCTGATCTC CCACTGCACC CCATGGATGA GTTGCCTGGG GGTCCCACCC 
     1261 CCATCACACC TGTCATCAAG GCTGGCTGGC TGGACAAGAA CCCACCACAG GGGTCTTATA 
     1321 TCTATCAGAA GCGATGGGTG AGACTGGACG CTGATTACCT GCGATACTTT GACAGTAACA 
     1381 AGGACGCCTA CTCTAAGCGC TTTGTTCCTG TGGCCTGCAT CTGCCGAGTA GCTCCTATTG 
     1441 GAGACCAGAA GTTTGAAGTG ATCACAAATA ATCGGACCTT CGCCTTCCGG GCAGAGAGTG 
     1501 ATGTGGAGCG GAACGAGTGG ATGCAGGCCC TGCAGCAGGC AGTAGTTGAG CATCGAGCCC 
     1561 GTTTTCGGCT TTCTAGTGCT TCTGTGTTGG GAGTCCGAGG CTCCGAGCAG CCTGACCGTG 
     1621 CTGGCAGCCT GGAGCTACGA GGCTTCAAGA ATAAGCTTTA CGTGGCTGTG ACTGGGGACA 
     1681 AAGTACAGCT CTATAAGAAT CTGGAGGAAT TCCATCTGGG CATTGGTATC ACCTTTATCG 
     1741 ACATGAATGT GGGCAATGTC AAGGAAGTGG ATCGGCGCAG CTTTGACCTC ACTACCCCCT 
     1801 ACCGCATCTT CAGCTTCTCG GCTGATTCAG AGTTAGAGAA GGAACAGTGG CTGGAGGCCA 
     1861 TGCAGGGAGC CATCGCAGAG GCCCTATCTA CCTCAGAGGT GGCTGAGCGC ATCTGGGCCG 
     1921 CAGCCCCCAA CAGGTTCTGT GCCGACTGTG GGGCCGCCCA GCCTGACTGG GCCTCCATCA 
     1981 ACCTCTGCGT TGTCATCTGC AAGCGCTGTG CAGGGGAGCA CCGCGGCCTG GGTGCAGGAG 
     2041 TGTCCAAGGT GCGGAGTTTG AAGATGGACA GGAAGGTGTG GACAGAAGCA CTCATCCAGC 
     2101 TCTTCTTACA TCTGGGCAAT GGCCCCGGGA ACCACTTCTG GGCTGCTAAT GTGCCTCCTA 
     2161 GTGAGGCCCT GGAGCCCAGC AGCAGCCCTG GTGCCCGGCG GTATCACCTG GAGGCCAAGT 
     2221 ACAGAGAGGG CAAGTACCGC CGCTACCATC CGCTCTTTGG CAACCAAGAG GAACTGGACA 
     2281 AGGCCCTGTG TGCTGCAGTT ACTACCACTG ACCTGGCTGA GACCCAGGCA CTCCTGGGCT 
     2341 GTGGGGCTGG GGTCAGCTGC TTCTCAGGGG ACCCAGCAGC TCCCACACCC CTGGCTCTTG 
     2401 CTGAGCAGGC CGGACAGACT CTGCAGATGG AGTTTCTACG GAATAATCAG AGTACAGAGG 
     2461 TCCCTCGGTT GGACTCAGTG AAGCCCTTGG AAAAGCACTA CTCCGTTACC CTGCCAACTG 
     2521 TGAGCCACAG CGGCTTCCTG TACAAAACTG CTTCTGCCGG CAAACCTCTG CAGGACCGCC 
     2581 GTGCCCGGGA AGAGTTCAGC CGGCGCTGGT GTGTCCTGAG TGATGGGGTC CTGAGCTACT 
     2641 ATGAAAACGA ACGGGCAGTG ACACCCAATG GGGAGATTCG GGCCAGCGAG ATAGTATGCC 
     2701 TAGCAGTTTC CCCTCTGGAC ACCCATGGCT TTGAGCACAC CTTTGAGGTG TACACAGAGG 
     2761 GAGAACGGCT GTACCTGTTT GGGCTGGAGA ATGCAGAGCT GGCTCATGAG TGGGTCAAGT 
     2821 GCATTGCCAA GGCGTTCGTG CCTCCCCTGG CTGAGGACCT GCTAGCCCGG GACTTTGAAC 
     2881 GTCTTGGGCG CCTACCCTGT AAAGCTGGCC TGAGCCTTCA GCAGGCTCAG GAAGGCTGGT 
     2941 TTGCCTTGAC TGGCTCTGAG CTCCGGGCTG TCTTCCCAGA GGGGCCCTGG GAAGAGCCGC 
     3001 TGCAGCTCCG GAAACTGCAA GAGCTTTCTA TCCAAGGAGA CAGCGAGAAC CAAGTGCTGG 
     3061 TGCTGGTGGA GCGGAGGAGG ACACTGTACA TCCAGGGTGA GCGGCGGCTG GACTTCATGG 
     3121 CTTGGCTAGG GGTCATCCAG AAAGCAGCAG CCAGCTTGGG AGACACACTA TCAGAACAAC 
     3181 AGCTTGGGGA CTCGGACATC CCAGTGATTG TGTACCGCTG TGTGGACTAC ATCACGCAGT 
     3241 GTGGCTTGAC CTCAGAGGGT ATCTATCGAA AGTGTGGTCA GACCTCCAAG ACTCAGAGAC 
     3301 TGCTAGACAG CCTCCGGCAG GACGCACGCT CTGTGCACCT AAAGGAGGGA GAACAGCACG 
     3361 TGGACGACGT CTCCTCTGCA CTCAAACGCT TCCTCAGGGA CCTGCCCGAT GGGCTCTTCA 
     3421 CGCGTGCGCA GCGCCTGGCC TGGCTGGAGG CCTCTGAGAT CGAGGATGAG GAAGAAAAGA 
     3481 TCTCCAGGTA TCGAGAGCTC TTGGTGCATC TGCCCCCTGT CAACCGGGCC ACTGTGAAGG 
     3541 CCCTTATCAG CCATCTGTAC TGTGTACAGT GCTTCTCAGA CACAAACCAA ATGAACACAC 
     3601 ACAACCTGGC TATCGTGTTT GGGCCTACAC TCTTCCAGAC AGACGGGCAG GACTACAAGG 
     3661 CCGGCAAGGT GGTGGAAGAC CTCATCAACC ACTACGTGGT GGTGTTCAGT GTGGACGAGG 
     3721 AGGAGCTGAG GAAGCAGAGG GAGGAAGTCA CGGCCATCGT GAAGATGCGA GTGGCTGGCA 
     3781 CTGCCAGTGG GACCCAGCAT GCTGGCGACT TCATCTGCAC AGTCTACCTG GAGGAGAAGA 
     3841 AGGTGGAGAC TGAACAGCAT GTTAAGATCC CAGCATCCAT GACTGCAGAG GAGCTTACTC 
     3901 TGGAGATTCT GGACCGCCGC AATGTGAGCA TCAGGGAGAA GGACTACTGG ACTTGCTTTG 
     3961 AGGTCAACGA GAAGGAGGAG GCAGAGCGCC CGCTGCACTT TGCAGAGAAG GTGCTGCCCA 
     4021 TTGTCCATGG GCTGGGCATA GACAGCCATC TGGTGGTGAA GAAGTACCAG TCCATGGAGG 
     4081 CCATGCTGTT GTACTTGGCC AGCCGTGTGG GTGACACCAA GCATGGTATG ATGAAGTTCC 
     4141 GTGAAGACCG CAGCCTCCTG GGTCTGGGGC TACCTTCGGG TGGCTTCCAC GATCGCTACT 
     4201 TCATTCTCAA CAGCAGCTGC CTACGGCTCT ACAAGGAGGT CCGGAGTCAC CGGCCTGAGA 
     4261 AGGAGTGGCC TGTCAAGAGC CTCAAAGTCT ACCTGGGTGT AAAGAAGAAA CTGCGGCCAC 
     4321 CTACTTGCTG GGGCTTCACG GTGGTGCACG AGACAGAAAA GCACGAGAAG CAGCAGTGGT 
     4381 ACCTCTGCTG TGACACGCAG ATGGAACTTC GAGAGTGGTT TGCCACCTTC CTCTCTGTGC 
     4441 AGCACGATGG CCTGGTGTGG CCCTCCGAGC CATCTCGAGT GTCCCGGGCA GTGCCTGAGG 
     4501 TCCGGATGGG CAGTGTATCG CTGATCCCTC TACGAGGCAG TGAAAATGAA ATGCGCCGGA 
     4561 GTGTGGCAGC CTTCACTGCT GACCCCCTTT CCCTTCTCCG CCATGTCAGC GGACCGACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//