LOCUS       MR231670                8454 bp    DNA     circular  SYN 
DEFINITION  Pan2 (myc-DDK-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2), transcript variant 2
ACCESSION   NM_001252326
VERSION     NM_001252326.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="Pan2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGAG GGTCTGGATC CTGGACTGGC CGAATTTTCC CCAGCTATGC 
     1081 ATTCTACCCT GGACCCAGTC CTGGATGCCC ACCTGAATCC AAGTTTGCTA CAGAATGTGG 
     1141 AGCTAGACCC AGAGGGAGTG GCCTTGGAGG CTCTTCCTGT CCAGGAGTCA GTGCACATAA 
     1201 TGGAAGGTGT CTACTCTGAG TTGCACAGCG TGGTGGCTGA AGTGGGAGTG CCTGTGTCTG 
     1261 TCTCCCACTT TGATTTACAT GAGGAGATGC TGTGGGTGGG GAGCCATGGG GGCCATGCCA 
     1321 CCTCCTTCTT CGGCCCAGCT CTGGAGCGCT ACTCATCCTT TCAGGTCAAT GGTGGTGACG 
     1381 ACATTCGGCA AATCCAGAGC CTGGAGAACG GTATCCTCTT CCTCACCAAG AACAACCTCA 
     1441 AGTACATGGC CCGTGGAGGG CTCATTATAT TTGACTATTT GCTGGATGAA AACGAGGATA 
     1501 TGCACAGCGT CCTCCTGACA GACAACAGCA CTCTGCTCGT TGGGGGGCTG CAGAACCACG 
     1561 TACTGGAGAT TGACCTGAAC ACTGTCCAGG AGACTCAGAA GTATGCAGTC GAGACACCCG 
     1621 GAGTCACCAT CATGAGACAG ACAAATCGTT TCTTCTTCTG TGGCCACACA TCTGGCAAGG 
     1681 TTTCCCTGCG AGACCTCCGT AGTTTTAAAG TGGAGCATGA ATTTGATGCC TTCTCAGGGA 
     1741 GTCTGTCAGA TTTTGATGTT CATGGCAACC TGCTGGCTGC CTGCGGCTTC TCCAGTCGCC 
     1801 TTACCGGCCT GGCCTGTGAC CGTTTCCTCA AAGTGTACGA TCTGCGCATG ATGCGTGCCA 
     1861 TCACACCTCT TCAAGTGCAC GTGGATCCGG CCTTCTTACG ATTCATCCCC ACATACACTT 
     1921 CCCGCCTTGC TATCATCTCC CAGTCAGGTC AATGCCAGTT TTGTGAACCC ACAGGCCTGG 
     1981 CCAACCCAGC CGACATTTTC CATGTGAATC CCGTGGGACC TTTGCTAATG ACGTTTGATG 
     2041 TGTCAGCCAG CAAGCAGGCC CTGGCCTTTG GGGATTCTGA GGGCTGCGTG CATCTCTGGA 
     2101 CTGATTCCCC TGAGCCGTCC TTCAACCCCT ACTCCCGAGA GACTGAGTTT GCCCTGCCCT 
     2161 GTCTTGTGGA CTCCTTGCCG CCTCTGGACT GGAGCCAGGA TCTGCTGCCG CTTTCCCTCA 
     2221 TTCCCGTCCC ACTTACCACT GATGCACTGC TCTCTGACTG GCCTGCTGCC AACTCTGCTC 
     2281 CCGCTCCCAG GCGAGCACCA CCTGTGGATG CAGAAATTCT TCGAACCATG AAGAAAGTGG 
     2341 GCTTCATTGG CTATGCTCCC AACCCTCGCA CCAGGCTGCG CAACCAGATT CCCTATCGAC 
     2401 TAAAGGAGTC AGACCATGAA TTTGACAACT TCAGCCAAGT CACGGAGTCA CCGACAGGGC 
     2461 GAGAAGAGGA GCCTCTCCAC ACAGTTTCTA AGAAATACCG GAAGGTAACC ATCAAATATT 
     2521 CCAAGCTAGG CCTGGAAGAC TTTGACTTCA AACACTACAA TAAGACTCTG TTTGCTGGGT 
     2581 TAGAGCCTCA CATCCCCAAT GCCTACTGTA ACTGCATGAT CCAGGTGCTC TATTTCTTGG 
     2641 AGCCTGTTCG CTGTCTGATC CAGAACCACC TTTGCCAAAA GGAGTTCTGC CTAGCGTGCG 
     2701 AGCTCGGCTT CCTCTTCCAC ATGCTAGACC TCTCTCGTGG CGATCCTTGT CAGGGCAGTA 
     2761 ATTTCCTCCG AGCTTTCCGG ACCATCCCTG AGGCCTCAGC TCTGGGTCTC ATCCTTGCTG 
     2821 ACTCAGATGA GGCTTCAGGC AAGGGCAGTC TGGCCCGGCT CATCCAGAGG TGGAACCGCT 
     2881 TCATCCTCAC TCAGCTACAT CAGGATATGC AGGAGCTGGA AGTGCCCCAG GCTTATCGAG 
     2941 GTGCTGGAGG CAGTTTCTGC TCATCAGGAG ACTCCATCAT TGGGCAGCTG TTCAGTTGTG 
     3001 AGATGGAGAA CTGCAGTCTT TGCCGCTGCG GCAGCGAGAC TGTGCGGGCC TCGTCCACCC 
     3061 TACTCTTCAC ACTCTCCTAC CCTGAGGATA AAACCGGGAA GAACTATGAC TTTGCTCAGG 
     3121 TGCTGAAGCG AAGTATCTGC CTGGAGCAGA ACACACAGGC CTGGTGTGAC AACTGTGAGA 
     3181 AGTACCAGCC CACAATTCAG ACCAGAAACA TCCGACATCT GCCAGATATT CTTGTCATTA 
     3241 ACTGTGAAGT GAATAGCTCA AAAGAGGCTG ATTTCTGGAG ACTGCAAGCT GAGGTTGCCT 
     3301 TCAAAATAGC GGTGAAAAAG TACGGCGGGG AAATGAAGAG CAAAGAGTTT GCTTTAGCTG 
     3361 ATCGGAAGGA ACTGAGGAGT CCAGAGGGCT TTCTGTGTTC CTCCATCGAG GAGCTGAAGA 
     3421 ATGTCTGGCT TCCATTTTCC ATCCGCATGA AGATGACCAA GAACAAAGGA CTGGATGTTT 
     3481 GCAACTGGGC TGACGAGCAT GAGTGGGGTC CGGCCAGGGC AGAGGAGGAG CTTGGTGTCT 
     3541 ATGTATATGA CCTGATGGCT ACTGTGGTAC ACATCCTGGA CTCACGAACA GGCGGCAGCT 
     3601 TGGTGGCTCA CATCAAAGTC GGTGAGACCT ACCACCAGCG CAAGGAGGGC GTGACTCATC 
     3661 AGCAGTGGTA TCTCTTCAAT GACTTCCTTA TTGAACCTAT TGATAAGTAT GAAGCCGTGC 
     3721 AGTTTGACAT GAATTGGAAA GTACCTGCTA TCCTGTATTA TGTCAAAAGG AATCTCAATT 
     3781 CCAGATACAA TCTGAATATC AAGAACCCCA TTGAGGCCAG TGTGCTGCTG GCAGAAGCCT 
     3841 CGCTGGCACG GAAGCAACGG AAAACACACA CGACCTTTAT TCCATTGATG CTGAATGAGA 
     3901 TGCCACAGGT CGGGGACCTG GTGGGCCTGG ATGCCGAGTT TGTCACTCTT AATGAGGAAG 
     3961 AAGCAGAGTT ACGCAGTGAT GGTACCAAGT CCACCATTAA GCCAAGCCAG ATGTCAGTAG 
     4021 CAAGGATCAC CTGTGTTCGG GGCCAAGGGC CTAACGAGGG CATCCCCTTC ATTGATGACT 
     4081 ACATCTCCAC GCAGGAGCAG GTAGTAGATT ACTTGACTCA GTACTCGGGG ATAAAGCCAG 
     4141 GAGACCTTGA TGCCAAAATC TCCTCAAAGC ACCTCACAAC CCTCAAGTCT ACCTACTTAA 
     4201 AGCTTCGCTT TCTCATTGAT ATTGGAGTCA AGTTTGTGGG TCATGGTCTG CAGAAGGACT 
     4261 TCCGGGTCAT CAACCTCATG GTGCCCAAGG ACCAAGTTCT TGACACAGTC TACCTGTTCC 
     4321 ACATGCCCCG AAAACGAATG ATTTCCCTAC GATTCCTGGC CTGGTATTTT CTAGACCTGA 
     4381 AGATTCAAGG TGAGACCCAT GACAGCATTG AGGATGCCCG CACAGCCCTT CAACTCTACC 
     4441 GGAAGTATCT GGAGCTGAGC AAGAACGGCA CCGAGCCCGA GTCCTTCCAC AAGGTGCTCA 
     4501 AGGGTCTCTA TGAGAAGGGC CGAAAGATGG ACTGGAAGGT GCCTGAGCCT GAAAGCCAGA 
     4561 CAAGTCCCAA GAATGCAGCT GTCTTCTCAG TGCTGGCACT GACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//