LOCUS       MR231668                8451 bp    DNA     circular  SYN 
DEFINITION  Tjp2 (myc-DDK-tagged) - Mouse tight junction protein 2 (Tjp2), transcript variant 1
ACCESSION   NM_001198985
VERSION     NM_001198985.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="Tjp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGCGG GGAGACCGCG GGTTCCCACC GCGGCGGGAG CTGTCGGGCT 
     1081 GGCAGCGCGG TCCAGGCATG GAGGAGGTGA TATGGGAGCA GTACACCGTG ACCCTGCAGA 
     1141 AGGATTCCAA AAGAGGATTT GGAATTGCCG TGTCGGGAGG CAGAGACAAC CCCCACTTTG 
     1201 AAAATGGGGA GACGTCAATC GTCATCTCAG ATGTGCTCCC AGGTGGACCC GCTGATGGCT 
     1261 TGCTTCAAGA AAATGACAGG GTGGTCATGG TCAATGGCAC CCCCATGGAA GATGTGCTCC 
     1321 ATTCGTTTGC CGTTCAGCAG CTTAGGAAAA GTGGGAAGAT AGCAGCCATC GTGGTCAAGA 
     1381 GACCCCGGAA GGTTCAGGTG GCCCCACTGC AAGGCAGCCC CCCTCTCAGT CATGACGACC 
     1441 GAGGTTTTGA AGTGATTGAA GAATTTGATG GCAGAAGTTT CCGCAGCGGC TACAGTGAGA 
     1501 GGAGTCGGCA CAGCAGCCAC GACATGCTCA GCCACAGCTG GGAGGGCAAT AGGGAGAGGG 
     1561 GGCGACCCCA TCAGCGCACA CAGAGCCGGG AGCGGGAGCG CAGCCGTGGC AGGAGCCTGG 
     1621 AACGGGGCCT GGACCAGGAA GACTATGGGC GCAGCCGTGA GCGCAGCCGT GGCCGGAGCC 
     1681 TGGAGCGCGG GCTGGACCGC GACTTTGTGT CCAGGGACCA CAGCCGTGGC CGTAGCATCG 
     1741 ACAGGGACTA CGACCGAGAC TATGAGCGCT CCTATCACGA AGCTTATGAA CCCGACTACG 
     1801 GAGGCGGCTA CAGCCCATCA TATGACCGTA GAGCCCATCC AGAGACCCGC TATGAACGGA 
     1861 GCCGCAGCCG AGAACACCTA CGTTCCCGAA GCCCCAGCCC CGAGTCTAGG TCACGGCATG 
     1921 AACACAAGGG CCAACATGAT CCCGACAGGC CCATCGGGGT CCTTCTAACC AAAAGCAAAG 
     1981 CAAATGAAGA GTATGGGCTC CGGCTGGGGA GTCAGATCTT CATCAAAGAA ATGACCAGAA 
     2041 CAGGACTGGC CACCAAAGAT GGCAACCTTC ATGAGGGGGA CATAATTCTC AAGATCAACG 
     2101 GCACTGTTAC TGAGAACATG TCTCTAACGG ATGCTCGGAA GTTAATAGAA AAGTCTCGAG 
     2161 GAAAACTGCA GCTTGTGGTG TTGAGAGACA GCAAGCAGAC CCTCATCAAC ATCCCAGCCC 
     2221 TAAACGACAG CGACTCTGAA GTGGAGGATA TCTCGGAAAT AGAGTCCAAC CGGTCTTTCT 
     2281 CTCCAGAGGA GAGACGCCAG CAGTATTCTG ATCAGGATTA CCATTCCTCC ACTGAGAAGC 
     2341 TGAAGGAGAG GCCGAGCTCA AGAGAGGAGA CCTCAGGCAG ACTATCCAGG ATGGGTGCCA 
     2401 CTCCCACGCC GTTCAAGTCC ACGGGGGACA TCACAGCTGC GGGGGTCACA GAGGCCAGCA 
     2461 GGGAGCCCAG GTACCAGGAA GAAGGCCCAG TTCCTCAACC CAGAACAGCT CCAAGAGTTT 
     2521 TTCTTCGTCC TAGTCCCGAA GATGAAGCAA TATATGGCCC TAACACCAAA ATGGTGAGGT 
     2581 TCAAGAAGGG AGACAGCGTG GGCCTCCGGT TGGCTGGTGG AAACGACGTT GGGATATTTG 
     2641 TGGCTGGCAT TCAGGAGGGC ACCTCCGCAG AGCAGGAGGG CCTGCAAGAA GGAGACCAGA 
     2701 TTCTGAAGGT GAACACACAG GATTTCAGAG GGCTAGTCCG GGAAGATGCG GTCCTCTACC 
     2761 TGCTAGAAAT CCCCAAAGGT GAAACTGTGA CCATTTTGGC TCAGAGCCGA GCAGACGTGT 
     2821 ACAGAGATAT CCTGGCTTGT GGCAGGGGAG ATTCGTTTTT CATAAGAAGC CACTTTGAAT 
     2881 GTGAGAAGGA AACTCCCCAG AGCCTGGCCT TCACCAGGGG AGAGGTCTTC CGAGTGGTAG 
     2941 ACACGCTGTA CGATGGCAAG CTGGGCCACT GGCTGGCCGT GAGGATCGGG AATGAGCTGG 
     3001 AGAAAGGCTT GATCCCTAAC AAAAGCAGAG CCGAGCAAAT GGCCAGTGTC CAGAATGCCC 
     3061 AGCGAGAGAA CGCCGGGGAC AGAGCAGACT TCTGGCGGAT GCGTGGCCAG AGATCCAGCG 
     3121 GCGGCGTCAA GAAGAACCTC CGCAAGAGCC GGGAAGACCT GGCGGCTGCT GTATCGGTTA 
     3181 GCACCAAGTT CCCTGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC AAGAGACCCG 
     3241 TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GTTGGCAACT GAGTTACCAG 
     3301 ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCCGG ATCTGAGAAA TCCAGTGGAG 
     3361 TGGTTCGGTT GAATACCGTG AGGCAAATTA TTGAGCAGGA CAAGCACGCC CTGCTCGACG 
     3421 TTACCCCCAA AGCTGTGGAC CTGCTCAACT ACACTCAGTG GTTCCCTATC GTGATTTTCT 
     3481 TCAACCCGGA TTCCAGACAA GGTGTTAAAA CCATAAGGCA GAGGTTGAGT CCAACATCCA 
     3541 ATAAAAGTTC TCGCAAGTTA TTTGATCAAG CCAACAAGCT CAAAAAGACC TGTTCTCATC 
     3601 TTTTTACAGC CACAATCAAC GTGAATTCAG CCAACGATGG CTGGTTTGGC AGCTTGAAGG 
     3661 ACAGCATTCA GCAGCAGCAA AACGAAGCAG TCTGGGTCTC TGAAGGAAAG ATGGAAGGGA 
     3721 TGGATGATGA TGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGCGCG GACTATCTGA 
     3781 GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGACACCGA CGGCGAGGGA GGCGCCTACA 
     3841 CTGACAATGA GCTAGAGGAG CCGGCGGAGG AGCCGCTGGT GTCTTCCATC ACCCGCTCCT 
     3901 CGGAGCCGGT GCAGCACGAG GAGAACATAA GGAAATCCAG CCCAGAGCCA CGAGCTCAGA 
     3961 TGAGGAGGGC TGCTAGCAGA GACCAACTTA GGGATGCTAG CCCGCCCCCA GCCTTCAAGC 
     4021 CAGAGCCGCC CAAGGCAAGA AGCCAGAACA GAGAAGACTC CTTCGACTAC TCCAAGTCAA 
     4081 ACCTCCCCGC CACAGCCGGC AGTGAAATCC CGGGGGGATC CACCAAAGGC TATCCTCCCC 
     4141 CTATTGCAGC GAAGCCTGCC TTTGGGCGGC CCATCCTGAA GCCTTCCACT CCAGTCCCTA 
     4201 TGCCTGAGAG CGAGGAGGTT GGAGAGAGCA CCGAGGAGCA GGAAGATGCT CCCAGATCAG 
     4261 TCCTGGGCAG AGTGAAAATA TTCGAGAAGA TGGACCACAA GGCAAAATTA CAGAGGATGC 
     4321 AGGAGCTCCA GGAGGCCCAG AATGCGAGGA TCGAAATAGC TCAGAAGCAC CCTGACATCT 
     4381 ATGCGGTTCC AATCAAGGCC CCCAAGCCAG ATGCTGGCCT CCCCCCGCAC ATGAGTTCTA 
     4441 GACCCCCAGA GCCCCAGAAA GCTCCTTCCA GACTTTACCA GGACACCAGA GGAAGCTACG 
     4501 GCAGTGACCC CGAGGAGGAG GAGTACCGCC AGCAGTTGGC AGCACACTCG AAGCGTGGTT 
     4561 ACTACAGCCA GCCCTCCCGG TACCGAGACA CGGAGTTAAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//