LOCUS       MR231665                8433 bp    DNA     circular  SYN 
DEFINITION  Cgn (myc-DDK-tagged) - Mouse cingulin (Cgn), transcript variant 2
ACCESSION   NM_001293727
VERSION     NM_001293727.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="Cgn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCTGAT CCCCGGGGCC CCGTAGACCA TGGAGTTCAA ATTCGATTTA 
     1081 TTACAGAGCC AGAGGGTGCT ACTGAAATGG GCACCCTTCG TCGTAGTGGG CGGCGCCCTG 
     1141 CAAGGGATGC AAGAGCCAGT ACCTATGGGG TCGCTGTGCG TGTGCAGGGC ATCGCCGGGC 
     1201 AACCGTTTGT GGTTCTTAAC AGTGGAGAGA AAGGGACTGA CTCCTTTGGG GTCCAGATCA 
     1261 AAGGGGGCAA CAACCGGGGG TCCCCAGGTG CACTGAGCTC CGATTCAGAG CTCCCCGAAA 
     1321 ACCCCTACTC TCAAGTCAAG GGACGCCCTG CCACTTCTCG GAGCAGCACA TCTGATGAGG 
     1381 AGCCCAAGGA CCACTTGAAT GGAAAACTGA TCCGTTCCCA GTCGCAAGCC TCTCTGACCG 
     1441 GGCTTGCCTT TATGAGTCCT AGTAATAGGA GCACCAGCTT GCTGGAACTA GCCCCTAAAC 
     1501 CGACTTCCTC GATTAACACC ATTGACACTG CCCCCTTGTC TTCAGTGGAC TCGCTCATCA 
     1561 ACAAGTTTGA TAGTCAGAAG GGGGGGCAGG TCCGGGGTCG CACTGGGCGC CGCACAAGGA 
     1621 CACTTCCCCA CGAACAGCGC AAGCGGAGCC AGAGTCTGGA CAGCAGGCTC CCACGGGATA 
     1681 CACGTGAGGA ACGGGAGCAC CAGTCCGCTA ACCACTGGAC CCGCGGCACA AAGTATGACA 
     1741 ACCATGTGGA CAGTTCAAAG AACCCATCGC AGAAGCAGAG CCCGTTCAGC AGCTTCAGCC 
     1801 GTTCGCGTCA GACTCAAGAC TGGGTGCTTC AGAGTTTTGA GGAGACACGG GATCCAGCCA 
     1861 TGGTGCAGTT CAAATCAACT CCAGACCTTC TCAGAGACCA GAGAGAGACA GCGCCACCAG 
     1921 GCAGTGCGGA CCATGTGAAG GCCACCATCT ATGGCATCCT AAGGGAGGGA AGCTCGGAGA 
     1981 GTGAGGCATC TGTGAGGAGG AAAGTTAGTT TGGTGCTGGA GCAAATGCAG CCTCTGGGGG 
     2041 CCCTGGCAGG ACAGGCTGAG CTTACCCGAA AAATGGAGGA ACTTCAGAAG AAGCTGGATG 
     2101 AGGAGGTGAA GAAGCGGCAG AAGCTAGAAC CATCCAGGGT TGGGCTGGAG AGGCAGCTGG 
     2161 AGGAGAAGGC TGAAGAGTGC CACCGACTGC AGGAGTTGCT GGAGAGGAGA AAGGGGGAGG 
     2221 TCCAGCAGAG CAGCAAGGAA CTTCAGAACA TGAAGCTCCT GCTAGGCCAG GAAGAAGGAC 
     2281 TACGGCATGG GCTGGAGGCC CAGGTGAAGG AGCTACAGCT TAAGCTCAAG CATAGCCAGA 
     2341 GTCCCGATTC TGGAAAGGAA AGTCTCCTAA AGGACTTGTT AGACACCCGG GAACTTCTAG 
     2401 AAGAACTCTT AGAGGGCAAA CAACGAGTAG AGGAGCAGCT CAGGCTGAGG GAACGGGAGC 
     2461 TGACAGCCCT GAAGGGGGCC CTGAAAGAGG AGGTGGCCTC CCACGACCAG GAGGTGGAAC 
     2521 ATGTCCGGCT GCAGTACCAG CGAGACACGG AGCAGCTTCG CCGGAGCATG CAAGATGCGA 
     2581 CCCAGGACCA TGCTGCACTG GAGGCTGAGA GGCAGAAGAT GTCTTCCCTC GTGCGGGAGC 
     2641 TGCAAAGAGA GCTAGAGGAG ACCTCTGAGG AGACAGGGCA TTGGCAGAGC ATGTTCCAGA 
     2701 AGAACAAGGA AGAGCTGAGA GCCACCAAGC AGGAACTGCT GCAACTGCGG ATGGAGAAGG 
     2761 AGGAGATGGA AGAGGAACTT GGGGAGAAGA TGGAGGTCTT ACAGAGGGAC TTAGAGCAGG 
     2821 CCCGAGCCAG TACAAGAGAT ACTCACCAGG TTGAGGAACT TAAAAAGGAG CTTCGACGCA 
     2881 CCCAGGGGGA GCTTAAAGAG CTTCAGGCAG AGCAGCAGAA CCAGGAGGTG ACTGGGCGAC 
     2941 ACCAGAACCA GGTGTTAGAG AAGCAGCTGG CAGCGCTGAG GGAGGAGGCT GATCGAGGCC 
     3001 GAGAGCTGGA GCAGCAGAAC CTCCAGCTAC AAAAGACCCT GCAGCAACTG CGGCAGGACT 
     3061 GTGAAGAGGC TTCCAAGGCT AAGGTGGCAT CAGAGACAGA GGCCATGGTG CTGGGGCAGC 
     3121 GTCGGGCAAC CGTGGAAACC ACACTTCGGG AGACACAAGA GGAAAATGAC GAATTCCGAA 
     3181 GGCGCATCCT GGGTCTGGAG CAGCAGTTGA AGGAGGCCCG AGGTCTGGCG GAAGGTGGGG 
     3241 AAGCGGTGGA GGCACGGCTT CGGGACAAAG TGCATCGGCT GGAGGTAGAG AAACAGCAGC 
     3301 TGGAAGAGGC TTTGAATGCT GCCCGGGAGG AGGAGGGGAA CCTGGCAGCA GCCAAGCGGG 
     3361 CTCTGGAGGT CCGGCTAGAT GAGGCCCAGC GGGGGCTGGC ACGCCTTGGG CAGGAACAGC 
     3421 AAGCACTGAA CCGGGCCCTA GAGGAAGAGG GGAAGCAGCG GGAGGCTCTC CGGAGGAGCA 
     3481 AGGCAGAGTT GGAGGAGCAG AAGCGTTTGC TGAACAGGAC TGTAGACCGA CTCAACAAAG 
     3541 AGCTGGAGCA GATTGGGGAT GACTCCAAGC TAGCCCTGCA GCAGCTCCAG GCTCAGATGG 
     3601 AAGATTACAA GGAAAAGGCC CGGAAGGAAG TAGCTGATGC CCAGCGTCAG GCCAAGGATT 
     3661 GGGCCAGTGA GGCTGAAAAG AATTCAGGGG GGCTGAGCCG GCTTCAGGAT GAGCTGCAAA 
     3721 GGCTGCGGCA AGCCCTGCAG ACATCCCAGG CTGAGCGGGA CACAGCCAGG CTGGACAAAG 
     3781 AGCTGCTGGC CCAGCGACTG CAGGGTCTAG AGCAAGAGGC TGAGAACAAG AAACGCTTCC 
     3841 AGGACGACAA GGCTCGACAG CTGAAGAGCC TAGAGGAGAA AGTCTCGCGG CTGGAAGCGG 
     3901 AGTTAGATGA AGAAAAGAAC ACTGTGGAAC TGTTAACAGA TCGGGTGAAC CGTGGCCGGG 
     3961 ATCAGGTGGA TCAACTGAGG ACAGAGCTCA TGCAGGAGAG ATCTGCTCGA CAGGACTTGG 
     4021 AGTGTGACAA AATCTCCTTG GAGAGACAGA ACAAGGATTT GAAGACCCGG TTGGCCAGTT 
     4081 CAGAGGGCTT CCAGAAACCC AGCGCTAGCC TCTCCCAGCT TGAGTCCCAG AATCAGCTGC 
     4141 TGCAAGAGCG GCTGCAAGCT GAAGAGAGAG AGAAGACAGT TCTGCAGTCC ACCAATCGCA 
     4201 AACTAGAACG GAGGGTTAAG GAGCTCTCCA TCCAGATTGA TGATGAACGG CAGCATGTCA 
     4261 ACGACCAGAA GGACCAGCTA ACCCTGAGGG TGAAGGCTTT GAAGCGGCAG GTGGATGAAG 
     4321 CAGAAGAGGA AATTGAGCGG CTGGACAGCC TGAGGAAGAA GGCACAGCGG GAACTGGAGG 
     4381 AACAGCATGA GGTCAATGAA CAGCTCCAAG CCCGGATCAA ATCCTTGGAA AAGGATGCCT 
     4441 GGCGGAAAGC TTCCCGCTCA GCTGCTGAGT CAGCCCTCAA ACAAGAGGGG CTGAGCTCTG 
     4501 ATGAGGAGTT TGACAACGTC TACGACCCCT CATCCATTGC ATCACTGCTT ACTGAGAGCA 
     4561 ACCTGCAGAC TAGCTCATGT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//