LOCUS       MR231663                8430 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 18
ACCESSION   NM_001253374
VERSION     NM_001253374.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4585..4676 
                     /label="Myc-DDK"  
     misc_feature    4706..5291 
                     /label="PolyA signal"  
     misc_feature    4763..4782 
                     /label="Restriction Enzyme"  
     misc_feature    5567..6239 
                     /label="ColE1"  
     misc_feature    6669..7463 
                     /label="Kan/Neo"  
     misc_feature    7498..7800 
                     /label="SV40 ori"  
     misc_feature    7886..8363 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4567 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGCGGAGG CAGCGGTCTT AGAATGAGCA GCAATATCCA CGCGAACCAT CTCAGCCTAG 
     1141 ACGCGTCCTC CTCCTCCTCT TCCTCCTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT ACCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCTT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTTT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGTTG TGTTAGTCTT TGCTCTCAGC ATTGGTGCCC 
     1441 TCGTAATATA CTTCATAGAC TCGTCAAACC CAATAGAATC CTGCCAGAAT TTCTACAAAG 
     1501 ATTTCACATT ACAGATCGAC ATGGCTTTCA ACGTGTTCTT CCTCCTCTAC TTTGGCTTGC 
     1561 GGTTTATTGC AGCCAACGAT AAGCTGTGGT TCTGGCTGGA AGTGAATTCA GTAGTAGATT 
     1621 TCTTCACAGT CCCTCCTGTG TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGCTTGA 
     1681 GATTTTTAAG AGCTCTCAGA CTGATACAGT TTTCAGAGAT TTTGCAGTTT CTGAATATCC 
     1741 TTAAAACAAG TAACTCCATC AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC 
     1801 TGACTGCAGC TGGATTCATC CACTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC 
     1861 AAAACAACCA GGCACTTACG TACTGGGAAT GTGTCTACTT ACTCATGGTC ACAATGTCTA 
     1921 CAGTGGGTTA TGGGGACGTT TATGCAAAAA CCACACTTGG ACGCCTCTTC ATGGTCTTCT 
     1981 TCATCCTCGG GGGACTGGCC ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG 
     2041 GAAACCGCAA GAAATACGGG GGCTCCTATA GCGCGGTTAG TGGAAGAAAG CACATTGTAG 
     2101 TCTGTGGACA CATTACTCTG GAGAGTGTCT CTAACTTCCT GAAGGACTTT CTGCACAAGG 
     2161 ACCGGGATGA TGTCAACGTG GAGATTGTCT TTCTTCACAA CATCTCCCCT AACCTTGAGC 
     2221 TTGAGGCTCT GTTCAAACGG CATTTCACTC AGGTGGAGTT TTATCAGGGC TCTGTCCTCA 
     2281 ATCCACATGA TCTTGCCAGA GTCAAGATAG AGTCAGCAGA TGCATGCCTG ATCCTTGCCA 
     2341 ATAAGTATTG CGCTGACCCG GATGCAGAAG ATGCCTCCAA CATCATGAGA GTGATCTCCA 
     2401 TCAAAAACTA CCACCCAAAG ATCAGGATCA TCACTCAGAT GCTGCAGTAT CACAACAAGG 
     2461 CCCATCTGCT CAACATCCCC AGCTGGAACT GGAAAGAGGG TGATGACGCA ATATGCCTTG 
     2521 CAGAGCTCAA GTTGGGTTTC ATAGCCCAGA GCTGTCTGGC TCAAGGCCTC TCCACAATGC 
     2581 TTGCCAATCT CTTCTCTATG AGGTCATTCA TAAAGATTGA GGAAGACACA TGGCAGAAAT 
     2641 ACTACTTGGA AGGAGTCTCC AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTCGTGG 
     2701 GTCTGTCCTT CCCTACTGTT TGTGAGCTGT GTTTTGTGAA GCTTAAGCTC CTGATGATAG 
     2761 CCATTGAGTA CAAGTCTGCC AACAGAGAGA GCCGAATATT AATTAACCCT GGGAACCACC 
     2821 TTAAGATCCA AGAAGGTACT TTAGGATTTT TCATCGCAAG TGATGCCAAA GAAGTTAAAA 
     2881 GGGCATTTTT TTACTGCAAG GCCTGTCATG ATGACGTCAC AGATCCCAAA AGAATTAAAA 
     2941 AATGTGGCTG CAGGCGGCTG ATCTATTTTG AAGATGAGCA GCCGCCAACC CTGTCACCAA 
     3001 AAAAAAAACA ACGTAATGGG GGCATGAGGA ACTCGCCCAA CACCTCCCCG AAGCTGATGA 
     3061 GGCATGACCC CTTGTTAATT CCTGGCAATG ATCAGATTGA CAACATGGAC TCCAATGTGA 
     3121 AAAAGTACGA CTCCACTGGA ATGTTTCACT GGTGTGCACC CAAGGAGATT GAGAAAGTCA 
     3181 TCTTGACTCG AAGTGAAGCT GCCATGACTG TCCTGAGTGG CCATGTCGTA GTCTGCATCT 
     3241 TTGGGGATGT CAGCTCAGCC CTGATTGGCC TCCGGAACCT GGTGATGCCA CTTCGTGCTA 
     3301 GCAACTTTCA CTATCATGAG CTCAAACACA TTGTGTTTGT GGGCTCCATT GAGTACCTCA 
     3361 AGAGGGAGTG GGAAACACTG CACAACTTCC CGAAAGTGTC CATATTGCCT GGTACACCAT 
     3421 TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG TGACATGTGC GTTATCCTGT 
     3481 CAGCCAATCA GAATAATATT GATGATACTT CGCTTCAGGA CAAGGAATGC ATCTTGGCGT 
     3541 CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATCGG GGTCTTGCAG GCTAATTCCC 
     3601 AAGGATTCAC ACCTCCTGGA ATGGACAGAT CATCACCCGA CAACAGCCCA GTGCACGGGA 
     3661 TGTTACGCCA GCCGTCCATC ACAACTGGGG TCAACATTCC CATCATCACG GAACTCGTGA 
     3721 ATGATACCAA TGTTCAGTTT TTGGACCAAG ACGATGACGA TGACCCTGAC ACAGAGCTGT 
     3781 ACCTCACACA GCCCTTTGCT TGTGGGACAG CATTTGCCGT CAGCGTCCTG GACTCACTCA 
     3841 TGAGCGCGAC ATACTTCAAT GACAATATCC TCACCCTAAT ACGGACCCTG GTGACAGGAG 
     3901 GAGCCACACC AGAGCTCGAG GCTCTAATAG CTGAGGAGAA TGCACTTCGA GGAGGCTACA 
     3961 GCACTCCGCA GACATTGGCC AACAGGGACC GTTGCCGAGT GGCCCAGTTA GCCCTGTTAG 
     4021 ATGGTCCCTT TGCAGACTTA GGGGATGGTG GTTGTTATGG TGATCTGTTC TGCAAAGCTC 
     4081 TGAAAACATA TAATATGCTT TGTTTTGGAA TTTACCGGCT GAGAGATGCC CACCTCAGCA 
     4141 CCCCCAGCCA GTGTACAAAA AGGTACGTCA TCACCAATCC TCCCTACGAG TTTGAGCTCG 
     4201 TACCAACAGA CCTGATCTTC TGCCTGATGC AGTTTGACCA CAACGCTGGC CAATCCCGGG 
     4261 CCAGTCTGTC TCATTCCTCC CACTCCTCAC AGTCGTCCAG TAAGAAGAGC TCCTCCGTCC 
     4321 ACTCCATCCC GTCCACAGCA AATCGGCCGA ACCGGCCCAA GTCCAGGGAG TCCCGCGACA 
     4381 AACAGAATGC AACAAGGATG ACTAGAATGG GCCAAGCAGA AAAGAAATGG TTTACAGATG 
     4441 AGCCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGTACC CACATGGCTA 
     4501 ACCAGATCAA CCAATATAAA TCCACAAGCA GCCTGATCCC TCCAATCAGA GAAGTTGAAG 
     4561 ATGAATGTAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4621 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4681 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4741 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4801 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4861 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4921 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4981 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5041 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5101 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5161 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5221 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5281 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5341 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5401 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5461 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5521 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5581 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5641 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5701 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5761 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5821 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5881 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5941 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6001 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6061 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6121 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6181 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6241 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6301 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6361 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6421 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6481 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6541 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6601 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6661 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6721 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6781 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6841 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6901 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6961 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7021 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7081 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7141 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7201 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7261 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7321 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7381 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7441 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7501 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7561 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7621 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7681 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7861 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7921 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7981 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8041 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8101 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8161 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8221 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8281 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8341 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8401 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//