LOCUS       MR231659                8415 bp    DNA     circular  SYN 
DEFINITION  Nek1 (myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), transcript variant 4
ACCESSION   NM_001293639
VERSION     NM_001293639.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Nek1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTGAGACTGC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CTGTTCTTGT TAAATCGACA GAGGATGGCA GACATTATGT CATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC TGATAAAGAA AGGCAAGAAT CAAGGAGAGA AGTTGCTGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAATATAAAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAA GGAGGTGATT TGTTTAAACG AATAAATGCT CAGAAAGGCG 
     1321 CTCTGTTTCA AGAAGACCAG ATTTTGGACT GGTTTGTGCA GATATGTTTG GCTCTGAAGC 
     1381 ATGTACATGA TAGAAAAATT CTTCACCGAG ACATAAAGTC ACAGAACATA TTTCTAACCA 
     1441 AAGATGGGAC AGTGCAGCTT GGAGATTTTG GAATTGCTCG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGCACTCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AGCCTTATAA CAATAAAAGT GACATTTGGG CTTTGGGCTG TGTCCTTTAT GAGTTGTGTA 
     1621 CACTTAAACA TGCATTTGAA GCTGGAAACA TGAAAAACCT GGTACTGAAG ATAATCTCCG 
     1681 GATCCTTTCC TCCAGTGTCT CCACATTACT CCTATGATCT CCGCAGCTTG CTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCTAAACG AATCGAAAAG TTTCTCTCCC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACACTTTC AAAGTTTGGA CCACAGCCTC TCCCAGGTAA AAGACCAGCA TCAGGACAAG 
     1921 GTGTCAGTTC TTTTGTCCCT GCTCAGAAAA TCACAAAGCC TGCTGCTAAA TACGGAGTGC 
     1981 CTTTAACATA TAAGAAGTAT GGAGATAAAA AGTTACTTGA GAAAAAACCA CCCCCAAAAC 
     2041 ATAAACAGGC CCATCAAATT CCCGTGAAGA AAATGAATTC TGGAGAAGAA AGGAAGAAAA 
     2101 TGTCTGAGGA AGCAGCAAAA AAAAGAAGGT TGGAATTTAT TGAGAAAGAA AAGAAGCAAA 
     2161 AGGATCAGAT TAGGTTCCTG AAGGCTGAGC AGATGAAGCG GCAAGAGAAG CAGCGGGCTT 
     2221 CCTTTTTTGG CATTGGAGGG GCTGTCTCTC CATCACCGTG TTCTCCTCGA GGCCAGTATG 
     2281 AACATTACCA TGCCATTTTT GACCAAATGC AGCGGCTAAG AGCAGAAGAT AATGAAGCAA 
     2341 GATGGAAGGG GGGAATCTAT GGTCGATGGC TCCCAGAAAG GCAAAAAGGA CACTTAGCTG 
     2401 TAGAGAGAGC CAACCAAGTG GAAGAATTCC TACAGCGTAA ACGAGAAGCT ATGCAGAATA 
     2461 AAGCCCGAGC CGAAGGACAC GTGGTTTATT TGGCAAGACT GAGGCAAATA AGACTACAAA 
     2521 ATTTTAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAGAATAAA GAAGCTGATG 
     2581 GTACCAAAGG ACAAGAAGCA ACTGAAGAGA CTGACATGAG GCTCAAAAAG ATGGAGTCAC 
     2641 TTAAGGCGCA AACAAATGCA CGTGCTGCTG TACTAAAAGA ACAGCTGGAG CGAAAAAGAA 
     2701 AGGAAGCTTA TGAAAGAGAA AAGAAAGTAT GGGAAGAACA TTTGGTGGCG AGGGTAAAAA 
     2761 GCTCAGATGT TCCTCTGCCT TTGGAACTTC TTGAAACAGG TGGTTCTCCA TCAAAGCAGC 
     2821 AGGTGAAGCC TGTCATTTCT GTGACTTCAG CTTTGAAAGA AGTGGGCCTG GATGGAAGTT 
     2881 TAACTGATAC CCAGGAAGAA GAAATGGAAA AGAGTAACAG TGCTATTTCA AGTAAGCGAG 
     2941 AAATCCTGCG TAGGCTAAAT GAAAATCTTA AAGCTCAAGA GGATGAAAAG GAAAAGCAGC 
     3001 ATCACTCAGG TTCTTGTGAG ACCGTTGGTC ACAAAGATGA GAGAGAGTAT GAGACAGAAA 
     3061 ATGCCATTTC CTCTGATCGC AAGAAGTGGG AGATGGGAGG TCAGCTTGTG ATTCCTCTCG 
     3121 ATGCAGTGAC ACTGGATACA TCCTTCTCTG CAACCGAAAA ACATACTGTG GGAGAGGTTA 
     3181 TTAAATTAGA TTCTAATGGC TCTCCAAGAA AAGTCTGGGG GAAAAACCCT ACAGATTCTG 
     3241 TGCTGAAGAT ACTTGGAGAA GCTGAATTAC AGCTACAGAC AGAACTACTA GAAAACACAT 
     3301 CTTTTAAAAG TGAGGTTTAT GCTGAAGAGG AGAACTACAA ACCCTTACTT ACTGAAGAAG 
     3361 AGAATCTGCA GTGCATTTCA AAAGAAATAA ATCCATCAGC TACTGTTGAT TCTACTGAAA 
     3421 CGAAAAGTCC AAAGTTTACT GAGGTGTCTC CACAAATGTC AGAAGGAAAT GTGGAAGAAC 
     3481 CTGATGATTT GGAAACAGAA GTTCTACAAG AGCCAAGTAG CACACACACA GATGGGAGTT 
     3541 TGCCACCTGT TCTTAATGAT GTGTGGACTA GAGAGAAGGA AGCAGCTAAG GAAACTGAGT 
     3601 TGGAAGATAA GGTTGCTGTG CAGCAGAGTG AAGTTTGTGA AGATAGAATT CCAGGGAACG 
     3661 TGGACCAATC CTGTAAGGAT CAGAGAGATC CTGCAGTAGA CGATTCTCCG CAGTCTGGCT 
     3721 GTGATGTAGA GAAGTCAGTA CAGCCAGAAT CGATTTTCCA GAAAGTGGTT CATTCTAAGG 
     3781 ACTTGAACTT AGTTCAGGCA GTTCATTGCT CACCAGAAGA ACCAATTCCA ATTCGATCTC 
     3841 ACTCTGATTC TCCACCAAAA ACTAAGAGCA AGAATTCCTT ACTGATTGGA CTTTCAACTG 
     3901 GTCTGTTTGA TGCAAACAAT CCAAAGATGC TGAGGACCTG CTCACTTCCA GATCTTTCCA 
     3961 AGCTGTTCAG AACCCTAATG GACGTTCCCA CTGTGGGGGA CGTTCATCAA GACAGTCTTG 
     4021 AAATCGATGA GCTGGAAGAT GAACCAATTA AAGAAGGGCC TTCTGATTCC GAAGACACTG 
     4081 TATTTGAAGA AACTGACACA GATTTACAAG AGCTTCAGGC CTCAATGGAG CAGCTGCTTA 
     4141 GGGAGCAACC AGGTGACGAA TACAGTGAGG AGGAAGAGTC TGTTTTAAAA AGCAGCGATG 
     4201 TGGAGCAGAC AGCAAGAGGG ACAGATGCCC CAGACGAGGA GGACAACCCC AGCAGCGAAA 
     4261 GCGCCCTGAA CGAGGAATGG CACTCAGATA ATAGTGACGC TGAGACCACT AGTGAATGTG 
     4321 AATATGACAG TGTCTTTAAC CATTTAGAGG AACTAAGACT TCACTTGGAG CAAGAAATGG 
     4381 GCTTTGAAAA GTTCTTTGAG GTTTATGAGA AAGTAAAGGC TATTCATGAG GATGAAGATG 
     4441 AAAATATTGA AATTTGTTCA ACAATAGTTG AGAATATTTT GGGCAATGAG CACCAGCATC 
     4501 TCTATGCCAA GATTCTGCAT TTAGTCATGG CAGATGGAGC CTATCAGGAA GATAATGATG 
     4561 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//