LOCUS       MR231658                8409 bp    DNA     circular  SYN 
DEFINITION  Prdm16 (myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 4
ACCESSION   NM_001291029
VERSION     NM_001291029.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4564..4655 
                     /label="Myc-DDK"  
     misc_feature    4685..5270 
                     /label="PolyA signal"  
     misc_feature    4742..4761 
                     /label="Restriction Enzyme"  
     misc_feature    5546..6218 
                     /label="ColE1"  
     misc_feature    6648..7442 
                     /label="Kan/Neo"  
     misc_feature    7477..7779 
                     /label="SV40 ori"  
     misc_feature    7865..8342 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4562 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Prdm16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGATCCAAG GCGAGGGCGA GGAAGCTAGC CAAAAGTGAC GGTGACGTTG 
     1081 TAAATAATAT GTATGAACCT GACCCGGACC TGCTGGCCGG CCAGAGTGCC GAGGAGGAGA 
     1141 CCGAAGACGG CATCCTGTCC CCCATCCCCA TGGGGCCACC GTCCCCCTTC CCCACCAGCG 
     1201 AGGACTTCAC TCCCAAGGAG GGCTCGCCCT ATGAGGCTCC TGTCTACATT CCTGAAGACA 
     1261 TTCCAATCCC ACCAGACTTC GAGCTACGAG AGTCCTCCAT ACCAGGAGCT GGCCTGGGGA 
     1321 TCTGGGCCAA GCGGAAGATG GAAATCGGGG AGAGGTTTGG CCCCTACGTG GTGACGCCCC 
     1381 GGGCCGCACT GAAGGAGGCC GACTTTGGAT GGGAGCAGAT GCTGACGGAT ACAGAGGTGT 
     1441 CATCCCAGGA GAGCTGCATC AAAAAGCAGA TCTCTGAAGA CTTGGGTAGC GAGAAGTTCT 
     1501 GCGTGGATGC CAATCAGGCG GGGTCTGGCA GCTGGCTCAA GTACATCCGT GTAGCGTGTT 
     1561 CCTGTGATGA CCAAAACCTC GCCATGTGTC AGATCAACGA ACAGATTTAC TATAAAGTCA 
     1621 TTAAGGACAT CGAGCCTGGA GAGGAACTGT TGGTGCATGT GAAAGAAGGT GCCTACTCCT 
     1681 TGGGTGTCAT GGCCCCCAGC TTGGATGAGG ACCCCACATT CCGCTGTGAT GAGTGTGATG 
     1741 AGCTCTTCCA GTGCAGGCTG GACCTGAGGC GCCACAAGAA GTACGCGTGC AGCTCTGCAG 
     1801 GAGCCCAGCT CTACGAGGGC CTAGGGGAGG AACTCAAGCC CGAGGGCCTT GGCGTGGGCA 
     1861 GCGACGGGCA AGCGCATGAG TGCAAGGATT GCGAGCGGAT GTTCCCCAAC AAGTACAGCT 
     1921 TGGAGCAACA CATGATCGTC CACACGGAAG AGCGTGAGTA CAAATGTGAC CAGTGTCCCA 
     1981 AGGCCTTCAA CTGGAAGTCC AACCTCATCC GCCACCAGAT GTCTCACGAC AGTGGCAAGC 
     2041 GCTTCGAATG TGAAAACTGT GTCAAGGTGT TCACGGACCC CAGCAACCTC CAGCGTCACA 
     2101 TCCGCTCACA GCATGTCGGT GCCCGGGCCC ATGCCTGCCC TGACTGTGGC AAGACCTTCG 
     2161 CCACATCCTC TGGCCTCAAA CAGCACAAGC ATATCCACAG CACGGTGAAG CCATTCATAT 
     2221 GCGAGGTCTG CCACAAGTCC TACACGCAGT TCTCCAACCT GTGCCGGCAC AAGCGGATGC 
     2281 ACGCCGACTG CAGGACGCAG ATCAAGTGCA AGGACTGTGG GCAGATGTTC AGCACTACCT 
     2341 CCTCCCTCAA CAAGCATCGG AGATTCTGCG AGGGCAAGAA CCATTACACG CCTGGCAGCA 
     2401 TCTTCACCCC AGGCCTGCCC TTGACCCCCA GCCCCATGAT GGACAAGACA AAACCCTCCC 
     2461 CGACCCTCAA CCACGGGGGC CTAGGCTTCA GCGAGTACTT CCCCTCCAGA CCTCATCCTG 
     2521 GGAGCCTGCC CTTCTCGGCT GCTCCTCCGG CCTTCCCCGC ACTCACTCCG GGCTTCCCGG 
     2581 GCATCTTTCC TCCATCCCTG TACCCACGAC CACCTCTGCT ACCTCCCACG CCGCTGCTCA 
     2641 AGAGCCCCCT GAACCACGCG CAGGACGCCA AGCTACCCAG CCCGCTGGGA AACCCAGCCC 
     2701 TGCCCCTTGT CTCCGCGGTC AGCAATAGCA GCCAGGGTGC CACAGCGGCC ACCGGGTCAG 
     2761 AGGAGAAATT TGATGGCCGC TTGGAAGACG CATATGCGGA GAAGGTCAAA AATAGGAGCC 
     2821 CTGACATGTC GGATGGCAGT GACTTTGAGG ATATCAACAC CACGACCGGG ACAGACTTGG 
     2881 ACACTACCAC GGGCACGGGG TCAGACCTGG ACAGCGACCT GGACAGTGAC AGAGACAAAG 
     2941 GCAAGGACAA GGGGAAGCCA GTGGAGAGCA AACCTGAGTT TGGGGGTGCA TCTGTGCCCC 
     3001 CTGGGGCCAT GAACAGTGTG GCCGAGGTAC CGGCCTTCTA CTCACAGCAT TCCTTCTTCC 
     3061 CGCCACCCGA GGAACAGCTG CTGACGGCCT CGGGAGCTGC CGGCGACTCC ATCAAGGCCA 
     3121 TCGCGTCCAT CGCGGAGAAA TACTTCGGTC CTGGCTTCAT GAGCATGCAG GAGAAGAAGC 
     3181 TGGGCTCACT ACCCTACCAC TCCGTGTTCC CCTTCCAGTT CCTGCCTAAC TTTCCCCACT 
     3241 CCCTCTACCC CTTTACGGAC CGAGCCCTCG CCCACAACTT GCTGGTCAAG GCTGAGCCAA 
     3301 AGTCACCCCG GGATGCCCTC AAGGTGGGCG GCCCCAGTGC GGAGTGCCCC TTCGACCTCA 
     3361 CCACCAAACC AAAAGAGGCC AAACCCGCCC TGCTCGCACC CAAGGTCCCC CTCATCCCCT 
     3421 CATCTGGCGA GGAACAGCCA CTGGACCTGA GCATCGGCAG CAGGGCCAGG GCAAGCCAGA 
     3481 ACGGAGGTGG CCGTGAGCCG CGGAAGAACC ACGTCTACGG TGAACGGAAG CCGGGGGTCA 
     3541 GCGAGGGGCT GCCTAAGGTG TGCCCAGCAC AGCTGCCCCA GCAGCCCTCC TTGCATTATG 
     3601 CTAAGCCTTC ACCGTTCTTC ATGGATCCCA TCTACAGCAG GGTAGAAAAG CGGAAGGTGG 
     3661 CAGACCCTGT GGGAGTCCTG AAAGAGAAGT ACCTGCGGCC GTCCCCACTT CTGTTCCACC 
     3721 CCCAGATGTC AGCCATAGAA ACCATGACGG AGAAGCTGGA GAGCTTTGCA GCCATGAAGG 
     3781 CCGACTCAGG CAGCTCCCTG CAGCCCCTGC CTCACCACCC GTTCAACTTC CGCTCCCCAC 
     3841 CCCCAACGCT CTCGGATCCC ATCCTCAGGA AGGGGAAGGA GAGATACACG TGCAGGTACT 
     3901 GTGGCAAGAT CTTCCCCAGA TCTGCAAATC TCACAAGACA TCTGAGGACA CACACAGGGG 
     3961 AGCAGCCATA CAGGTGCAAG TACTGTGACC GGTCATTCAG CATCTCCTCC AACCTCCAGC 
     4021 GGCACGTGAG GAACATCCAC AACAAAGAGA AGCCGTTCAA GTGCCATCTG TGCAACCGCT 
     4081 GCTTCGGGCA GCAGACCAAC CTAGACCGGC ACCTGAAGAA GCACGAACAC GAGGGCGCAC 
     4141 CAGTGAGCCA GCACTCCGGG GTGCTCACGA ACCACCTGGG CACCAGCGCC TCCTCCCCCA 
     4201 CCTCCGAGTC GGACAACCAT GCACTTTTAG ATGAGAAGGA AGATTCTTAC TTCTCCGAGA 
     4261 TCCGAAACTT CATCGCCAAC AGCGAGATGA ACCAGGCATC CACTCGAATG GACAAACGGC 
     4321 CTGAGATCCA AGACCTGGAC AGCAACCCAC CGTGTCCAGG CTCAGCCAGT GCAAAGCCAG 
     4381 AGGACGTAGA GGAGGAGGAA GAGGAGGAGC TGGAGGAAGA GGATGATGAC AGCTTAGCCG 
     4441 GGAAGTCACA GGAGGACACG GTGTCCCCCA CACCTGAGCC CCAAGGAGTC TATGAAGATG 
     4501 AAGAGGATGA GGAACCACCC AGCCTGACCA TGGGCTTTGA CCATACCCGG AGGCATATGC 
     4561 AAACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4621 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6181 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6241 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6301 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6361 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6421 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6481 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6541 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6601 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6661 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6721 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6781 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6841 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6901 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6961 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7021 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7081 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7141 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7201 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7261 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7321 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7381 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7441 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7501 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7561 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7621 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7861 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7921 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7981 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8041 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8101 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8161 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8221 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8281 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8341 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8401 GCGAATTTT
//