LOCUS       MR231656                8412 bp    DNA     circular  SYN 
DEFINITION  Spag9 (myc-DDK-tagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 7
ACCESSION   NM_001199205
VERSION     NM_001199205.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4569..4660 
                     /label="Myc-DDK"  
     misc_feature    4690..5275 
                     /label="PolyA signal"  
     misc_feature    4747..4766 
                     /label="Restriction Enzyme"  
     misc_feature    5551..6223 
                     /label="ColE1"  
     misc_feature    6653..7447 
                     /label="Kan/Neo"  
     misc_feature    7482..7784 
                     /label="SV40 ori"  
     misc_feature    7870..8347 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4551..4557 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4551 
                     /label="Spag9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCCTGGA TGCATGCTGC TGTTTGTGTT TGGCTTTGTT GGCGGGGCGG 
     1081 TGGTCATTAA TTCTGCGATC TTAGTATCTC TCTCTGTTTT GCTGCTTGTG CACTTTTCTA 
     1141 TTTCTACCGG GGTGCCAGCG CTGACGCAGA ACCTACCAAG GATACTCAGA AAAGAACGTC 
     1201 CTATATCATT AGGGATTTTC CCTCTACCTG CTGGAGATGG ATTGCTTACA CCTGACACTC 
     1261 AGAAAGGAGG CGAGACCCCA GGATCAGAGC AATGGAAATT TCAAGAATTA AGTCAACCAC 
     1321 GTTCTCATAC CAGCCTGAAG GATGAACTTT CTGATATTAG TCAAGGTGGA TCTAAAGCTA 
     1381 CCACTCCAGC TTCAACAGCA AATTCAGACG TATCAGCAAT TCCTCCTGAT ACTCCGTCAA 
     1441 AGGAAGATAA TGAAGGATTT GTAAAAGGCA CAGATACATC AAATAAGTCA GAGATAAGCA 
     1501 AACACATAGA AGTCCAGGTT GCCCAAGAGA CTAGGAATGT GTCTACAGAA TCTGGTGAAA 
     1561 ATGAAGAGAA ATCAGAAGTT CAAGCAATCA TTGAATCCAC TCCTGAATTG GATATGGACA 
     1621 AAGACCTCAG TGGATATAAA GGTTCAAGCA CTCCCACCAA AGGCATAGAG AACAAAGCTT 
     1681 TTGATCGCAA TACAGAATCT CTCTTTGAAG AGCTGTCCTC AGCTGGCTCG GGCCTTATTG 
     1741 GAGATGTGGA TGAAGGAGCG GATTTACTAG GAATGGGTCG TGAGGTGGAG AATCTCATAC 
     1801 TGGAGAACAC ACAGCTGCTG GAAACCAAAA ACGCTCTGAA TGTAGTGAAG AATGACTTAA 
     1861 TAGCCAAAGT GGATGAGCTG ACCTGTGAGA AGGACGTGCT GCAGGGGGAG CTGGAGGCTG 
     1921 TGAAGCAAGC CAAGCTGAAG CTGGAGGACA AGAACAGAGA GCTGGAGGAG GAGCTCAGGA 
     1981 AAGCTCGTGC AGAAGCTGAA GATGCAAGGC AAAAAGCAAA AGACGATGAT GATAGTGACA 
     2041 TTCCCACAGC CCAGAGGAAG AGGTTTACCA GAGTAGAAAT GGCCCGAGTT CTCATGGAGA 
     2101 GAAACCAGTA CAAAGAGAGG CTGATGGAGC TTCAAGAAGC TGTTCGATGG ACGGAGATGA 
     2161 TCCGGGCCTC ACGAGAGAAC CCAGCTATGC AAGAAAAGAA AAGGTCAAGC ATCTGGCAAT 
     2221 TTTTCAGCCG ACTTTTTAGC TCTTCAAGTA ATGCAACAAA GAAGCCTGAA CCCCCTGTTA 
     2281 ACCTGAAGTA CAACGCCCCC ACCTCTCACG TGACTCCCTC CGTGAAGAAA AGGAGCAGCA 
     2341 CTTTATCTCA GCTCCCTGGG GACAAGTCCA AGGCATTTGA TTTCCTCAGT GAGGAAACTG 
     2401 AGGCTAGTTT AGCCTCCCGC AGAGAGCAAA AGAGAGAGCA GTATCGGCAG GTGAAGGCTC 
     2461 ACGTTCAGAA GGAAGATGGC CGAGTGCAAG CTTTCGGCTG GAGTCTGCCT CAGAAGTACA 
     2521 AACAGGTAGC CAATGGTCAA GGGGAAACTA AGATGAAAAA TTTACCTGTG CCTGTCTATC 
     2581 TCAGACCACT AGACGAAAAA GATGCGTCTA TGAAGCTGTG GTGTGCTGTA GGAGTCAATT 
     2641 TATCTGGTGG CAAGACAAGA GATGGCGGGT CTGTTGTTGG AGCGAGTGTA TTTTACAAGG 
     2701 ATATTGCTGG TTTGGACACT GAAGGCAGTA AACAGCGGAG TGCGTCTCAG AGTAGTTTAG 
     2761 ACAAGTTGGA TCAAGAACTC AAGGAACAGC AGAAAGAATT TAAAAATCAA GAAGAGTTGT 
     2821 CCAGTCAGGT CTGGATCTGT ACCAGCACCC ACTCAACTAC AAAGGTTATC ATCATTGATG 
     2881 CTGTTCAGCC TGGCAACATC CTAGATAGTT TCACTGTTTG CAATTCTCAT GTTCTGTGCA 
     2941 TTGCCAGTGT CCCAGGAGCT CGGGAAACAG ACTACCCTGC AGGAGAAGAA CTTTCTGAAT 
     3001 CTGGTCAGGT AGACAAAGCG TCATTGTGTG GAAGCATGAC CAGTAACAGC TCCGCAGAGA 
     3061 TGGACAGCTT GCTGGGAGGC ATCACAGTGG TTGGGTGCTC CACAGAAGGA CTGACAGGAG 
     3121 CTGCCACTTC CCCAAGTACC AACGGTGCTT CTCCTGTGAT TGAAAAGCCA CCAGAAATGG 
     3181 AAACTGAAAA TAGCGAAGTT GATGAAAATA TTCCAACAGC AGAAGAAGCA ACTGAAGCCA 
     3241 CAGAGGGCAA TGCAGGGTCC ACTGAAGACA CTGTGGACAT CTCCCAGCCT GGCGTGTACA 
     3301 CAGAGCATGT GTTTACAGAT CCACTGGGAG TTCAGATCCC AGAAGACCTC TCCCCAGTGT 
     3361 TTCAGTCAAG TAATGACTCA GATGTGTACA AAGATCAAAT ATCAGTATTG CCAAATGAAC 
     3421 AAGACCTTGC GAGAGAAGAA GCTCAGAAAA TGAGTAGTCT TTTACCAACC ATGTGGCTTG 
     3481 GAGCTCAGAA TGGCTGTTTG TATGTCCACT CATCTGTAGC CCAGTGGAGG AAATGTCTCC 
     3541 ATTCCATTAA GCTCAAAGAC TCAATTCTCA GTATTGTACA TGTGAAAGGA ATTGTACTAG 
     3601 TGGCCCTGGC GGATGGCACC CTTGCAATCT TCCACAGAGG AGTTGATGGA CAATGGGATC 
     3661 TGTCAAACTA TCACCTTTTA GACCTTGGAC GTCCTCATCA TTCCATACGA TGCATGACTG 
     3721 TGGTACATGA CAAAGTCTGG TGTGGCTATA GGAACAAAAT CTATGTGGTT CAACCAAAGG 
     3781 CTATGAAGAT AGAGAAATCA TTTGATGCAC ACCCCAGGAA GGAGAGCCAA GTACGGCAGC 
     3841 TTGCATGGGT GGGTGATGGT GTATGGGTCT CCATTCGTTT GGATTCCACG CTCCGCCTCT 
     3901 ATCATGCACA CACATACCAA CATCTCCAGG ATGTGGATAT TGAGCCTTAT GTAAGCAAAA 
     3961 TGTTAGGTAC TGGAAAACTG GGTTTCTCTT TCGTGAGGAT AACAGCTCTT ATGGTGTCTT 
     4021 GCAATCGTTT GTGGGTGGGG ACTGGAAATG GTGTCATTAT CTCCATCCCA CTGACAGAAA 
     4081 CCGTAATCCT CCACCAGGGA CGTTTACTGG GGCTAAGGGC AAATAAAACC TCAGGGACTC 
     4141 CAGGTAACCG TCCTGGAAGT GTAATCCGTG TATATGGTGA TGAGAACAGT GATAAAGTGA 
     4201 CTCCAGGGAC ATTTATACCC TACTGTTCAA TGGCACATGC ACAGCTTTGT TTCCACGGGC 
     4261 ACCGGGATGC TGTGAAATTC TTTGTGGCAG TCCCAGGTCA GGTCATTAGC CCACAAAGTA 
     4321 GCAGTGGTGG GGCAGATCTT ACAGCTGACA AGGCAGGGTC ATCTGCACAG GAGCCCAGTA 
     4381 GCCAGACGCC CTTGAAGTCC ATGCTTGTCA TCAGTGGAGG AGAGGGCTAC ATTGACTTCC 
     4441 GGATGGGTGA TGAAGGTGGA GAATCTGAAC TTCTGGGAGA GGATCTTCCA CTGGAACCTT 
     4501 CAGTCACCAA AGCCGAGAGG AGTCACCTGA TAGTGTGGCA AGTGATGTGT GGCAATGAAA 
     4561 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4621 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5341 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5401 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5461 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5521 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5581 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5641 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5701 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5761 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5821 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5881 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5941 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6001 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6181 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6241 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6301 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6361 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6421 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6481 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6541 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6601 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6661 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6721 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6781 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6841 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6901 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6961 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7021 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7081 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7141 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7201 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7261 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7321 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7381 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7441 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7501 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7561 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7621 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8401 AACGCGAATT TT
//