LOCUS       MR231653                8400 bp    DNA     circular  SYN 
DEFINITION  Pan2 (myc-DDK-tagged) - Mouse PAN2 polyA specific ribonuclease subunit homolog (S. cerevisiae) (Pan2), transcript variant 3
ACCESSION   NM_001252327
VERSION     NM_001252327.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4557..4648 
                     /label="Myc-DDK"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    4735..4754 
                     /label="Restriction Enzyme"  
     misc_feature    5539..6211 
                     /label="ColE1"  
     misc_feature    6641..7435 
                     /label="Kan/Neo"  
     misc_feature    7470..7772 
                     /label="SV40 ori"  
     misc_feature    7858..8335 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="Pan2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTTGAG GGTCTGGATC CTGGACTGGC CGAATTTTCC CCAGCTATGC 
     1081 ATTCTACCCT GGACCCAGTC CTGGATGCCC ACCTGAATCC AAGTTTGCTA CAGAATGTGG 
     1141 AGCTAGACCC AGAGGGAGTG GCCTTGGAGG CTCTTCCTGT CCAGGAGTCA GTGCACATAA 
     1201 TGGAAGGTGT CTACTCTGAG TTGCACAGCG TGGTGGCTGA AGTGGGAGTG CCTGTGTCTG 
     1261 TCTCCCACTT TGATTTACAT GAGGAGATGC TGTGGGTGGG GAGCCATGGG GTCAATGGTG 
     1321 GTGACGACAT TCGGCAAATC CAGAGCCTGG AGAACGGTAT CCTCTTCCTC ACCAAGAACA 
     1381 ACCTCAAGTA CATGGCCCGT GGAGGGCTCA TTATATTTGA CTATTTGCTG GATGAAAACG 
     1441 AGGATATGCA CAGCGTCCTC CTGACAGACA ACAGCACTCT GCTCGTTGGG GGGCTGCAGA 
     1501 ACCACGTACT GGAGATTGAC CTGAACACTG TCCAGGAGAC TCAGAAGTAT GCAGTCGAGA 
     1561 CACCCGGAGT CACCATCATG AGACAGACAA ATCGTTTCTT CTTCTGTGGC CACACATCTG 
     1621 GCAAGGTTTC CCTGCGAGAC CTCCGTAGTT TTAAAGTGGA GCATGAATTT GATGCCTTCT 
     1681 CAGGGAGTCT GTCAGATTTT GATGTTCATG GCAACCTGCT GGCTGCCTGC GGCTTCTCCA 
     1741 GTCGCCTTAC CGGCCTGGCC TGTGACCGTT TCCTCAAAGT GTACGATCTG CGCATGATGC 
     1801 GTGCCATCAC ACCTCTTCAA GTGCACGTGG ATCCGGCCTT CTTACGATTC ATCCCCACAT 
     1861 ACACTTCCCG CCTTGCTATC ATCTCCCAGT CAGGTCAATG CCAGTTTTGT GAACCCACAG 
     1921 GCCTGGCCAA CCCAGCCGAC ATTTTCCATG TGAATCCCGT GGGACCTTTG CTAATGACGT 
     1981 TTGATGTGTC AGCCAGCAAG CAGGCCCTGG CCTTTGGGGA TTCTGAGGGC TGCGTGCATC 
     2041 TCTGGACTGA TTCCCCTGAG CCGTCCTTCA ACCCCTACTC CCGAGAGACT GAGTTTGCCC 
     2101 TGCCCTGTCT TGTGGACTCC TTGCCGCCTC TGGACTGGAG CCAGGATCTG CTGCCGCTTT 
     2161 CCCTCATTCC CGTCCCACTT ACCACTGATG CACTGCTCTC TGACTGGCCT GCTGCCAACT 
     2221 CTGCTCCCGC TCCCAGGCGA GCACCACCTG TGGATGCAGA AATTCTTCGA ACCATGAAGA 
     2281 AAGTGGGCTT CATTGGCTAT GCTCCCAACC CTCGCACCAG GCTGCGCAAC CAGATTCCCT 
     2341 ATCGACTAAA GGAGTCAGAC CATGAATTTG ACAACTTCAG CCAAGTCACG GAGTCACCGA 
     2401 CAGGGCGAGA AGAGGAGCCT CTCCACACAG TTTCTAAGAA ATACCGGAAG GTAACCATCA 
     2461 AATATTCCAA GCTAGGCCTG GAAGACTTTG ACTTCAAACA CTACAATAAG ACTCTGTTTG 
     2521 CTGGGTTAGA GCCTCACATC CCCAATGCCT ACTGTAACTG CATGATCCAG GTGCTCTATT 
     2581 TCTTGGAGCC TGTTCGCTGT CTGATCCAGA ACCACCTTTG CCAAAAGGAG TTCTGCCTAG 
     2641 CGTGCGAGCT CGGCTTCCTC TTCCACATGC TAGACCTCTC TCGTGGCGAT CCTTGTCAGG 
     2701 GCAGTAATTT CCTCCGAGCT TTCCGGACCA TCCCTGAGGC CTCAGCTCTG GGTCTCATCC 
     2761 TTGCTGACTC AGATGAGGCT TCAGGCAAGG GCAGTCTGGC CCGGCTCATC CAGAGGTGGA 
     2821 ACCGCTTCAT CCTCACTCAG CTACATCAGG ATATGCAGGA GCTGGAAGTG CCCCAGGCTT 
     2881 ATCGAGGTGC TGGAGGCAGT TTCTGCTCAT CAGGAGACTC CATCATTGGG CAGCTGTTCA 
     2941 GTTGTGAGAT GGAGAACTGC AGTCTTTGCC GCTGCGGCAG CGAGACTGTG CGGGCCTCGT 
     3001 CCACCCTACT CTTCACACTC TCCTACCCTG AGGATAAAAC CGGGAAGAAC TATGACTTTG 
     3061 CTCAGGTGCT GAAGCGAAGT ATCTGCCTGG AGCAGAACAC ACAGGCCTGG TGTGACAACT 
     3121 GTGAGAAGTA CCAGCCCACA ATTCAGACCA GAAACATCCG ACATCTGCCA GATATTCTTG 
     3181 TCATTAACTG TGAAGTGAAT AGCTCAAAAG AGGCTGATTT CTGGAGACTG CAAGCTGAGG 
     3241 TTGCCTTCAA AATAGCGGTG AAAAAGTACG GCGGGGAAAT GAAGAGCAAA GAGTTTGCTT 
     3301 TAGCTGATCG GAAGGAACTG AGGAGTCCAG AGGGCTTTCT GTGTTCCTCC ATCGAGGAGC 
     3361 TGAAGAATGT CTGGCTTCCA TTTTCCATCC GCATGAAGAT GACCAAGAAC AAAGGACTGG 
     3421 ATGTTTGCAA CTGGGCTGAC GAGCATGAGT GGGGTCCGGC CAGGGCAGAG GAGGAGCTTG 
     3481 GTGTCTATGT ATATGACCTG ATGGCTACTG TGGTACACAT CCTGGACTCA CGAACAGGCG 
     3541 GCAGCTTGGT GGCTCACATC AAAGTCGGTG AGACCTACCA CCAGCGCAAG GAGGGCGTGA 
     3601 CTCATCAGCA GTGGTATCTC TTCAATGACT TCCTTATTGA ACCTATTGAT AAGTATGAAG 
     3661 CCGTGCAGTT TGACATGAAT TGGAAAGTAC CTGCTATCCT GTATTATGTC AAAAGGAATC 
     3721 TCAATTCCAG ATACAATCTG AATATCAAGA ACCCCATTGA GGCCAGTGTG CTGCTGGCAG 
     3781 AAGCCTCGCT GGCACGGAAG CAACGGAAAA CACACACGAC CTTTATTCCA TTGATGCTGA 
     3841 ATGAGATGCC ACAGGTCGGG GACCTGGTGG GCCTGGATGC CGAGTTTGTC ACTCTTAATG 
     3901 AGGAAGAAGC AGAGTTACGC AGTGATGGTA CCAAGTCCAC CATTAAGCCA AGCCAGATGT 
     3961 CAGTAGCAAG GATCACCTGT GTTCGGGGCC AAGGGCCTAA CGAGGGCATC CCCTTCATTG 
     4021 ATGACTACAT CTCCACGCAG GAGCAGGTAG TAGATTACTT GACTCAGTAC TCGGGGATAA 
     4081 AGCCAGGAGA CCTTGATGCC AAAATCTCCT CAAAGCACCT CACAACCCTC AAGTCTACCT 
     4141 ACTTAAAGCT TCGCTTTCTC ATTGATATTG GAGTCAAGTT TGTGGGTCAT GGTCTGCAGA 
     4201 AGGACTTCCG GGTCATCAAC CTCATGGTGC CCAAGGACCA AGTTCTTGAC ACAGTCTACC 
     4261 TGTTCCACAT GCCCCGAAAA CGAATGATTT CCCTACGATT CCTGGCCTGG TATTTTCTAG 
     4321 ACCTGAAGAT TCAAGGTGAG ACCCATGACA GCATTGAGGA TGCCCGCACA GCCCTTCAAC 
     4381 TCTACCGGAA GTATCTGGAG CTGAGCAAGA ACGGCACCGA GCCCGAGTCC TTCCACAAGG 
     4441 TGCTCAAGGG TCTCTATGAG AAGGGCCGAA AGATGGACTG GAAGGTGCCT GAGCCTGAAA 
     4501 GCCAGACAAG TCCCAAGAAT GCAGCTGTCT TCTCAGTGCT GGCACTGACG CGTACGCGGC 
     4561 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4621 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5341 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5401 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5461 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5521 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5581 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5641 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5701 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5761 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5821 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5881 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5941 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6001 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6061 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6121 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6181 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6241 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6301 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6361 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6421 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6481 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6541 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6601 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6661 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6721 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6781 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6841 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6901 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6961 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7021 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7081 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7141 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7201 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7261 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7321 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7381 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7441 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7501 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7561 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7621 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7801 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7861 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7921 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7981 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8041 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8101 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8161 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8221 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8281 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8341 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//