LOCUS       MR231652                8406 bp    DNA     circular  SYN 
DEFINITION  Nrde2 (myc-DDK-tagged) - Mouse nrde-2 necessary for RNA interference, domain containing (Nrde2), transcript variant 1
ACCESSION   NM_001290303
VERSION     NM_001290303.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4561..4652 
                     /label="Myc-DDK"  
     misc_feature    4682..5267 
                     /label="PolyA signal"  
     misc_feature    4739..4758 
                     /label="Restriction Enzyme"  
     misc_feature    5543..6215 
                     /label="ColE1"  
     misc_feature    6645..7439 
                     /label="Kan/Neo"  
     misc_feature    7474..7776 
                     /label="SV40 ori"  
     misc_feature    7862..8339 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4543 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="Nrde2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGTTC CCGGCTTTTG CGGACGTAAG TGAGGCCTCC GGTGATGGGG 
     1081 CATTCAGGAA AGAGTTAGAT TGGCTGAGCA ACCCAAGCTT TCGTGTTGGA AATCTAACTT 
     1141 CTCTGAGCCG ACAAACTGAA GAGGTCACAG CCCTTGCTTC TGAAGGGTCA CCACCGCCAA 
     1201 GGTACTCTTT TATCAGGAGT CCTCTGAAGT CAGAGCTCTC AGGTGAGAGC AACACTAGCG 
     1261 AGAAGCTCGC ACAGACAAGC AGGAAGAAGA AGAAGGAGAA AAAGAAGAGA CGGAAGCATC 
     1321 AGCACCACAG GAAAACCAAG AGGAGGCACG AGCAGTTGAG CAGCAGCGGC TCTGAGTCAG 
     1381 ACACAGAGGC TGGGAAGGAC AGGGCTTCCC GGAGCATCAG GGACGACCAG AAGGAAGCCG 
     1441 AGAAACCCTG TCAGGGAAGC AATGCGGCTG CTGCTGTTGC TGCTGCTGCT GGACATCGGT 
     1501 CCATTTGGCT GGAAGATATT CACGATCTGA CCGACGTCTT CAGGACTGAT AAGAAGCCAG 
     1561 ATCCTGCCAA CTGGGAGTAT AAATCTCTGT ATCGTGGGGA TATAGCAAGG TACAAGAGGA 
     1621 AGGGAGACTC CTGCCTGGGT ATCAATCCTA AGAAACAGTG TATATCTTGG GAAGGGGCTT 
     1681 CTGCAGCAAA GAAGCACTCA CACAGGCACC TGGAGCGCTA TTTTACCAAG AAGAATGTGG 
     1741 GGTTAATGAG AACAGAGGGC ATTGCTGTGT GCAGTAACCC CGAGCCTGCC TCCTCCGAGC 
     1801 CCGTCACATT CATCCCAGTG AAGGACTCTG CTGAGGCAGC TACTCCTGTT ACATCATGGC 
     1861 TGAATCCTCT GGGGATTTAT GATCAGTCTA CCACACAGTG GTTACAAGGA CAGGGTCCAG 
     1921 CAGAGCAAGA GTCAAAGCAG CCGGACTCGC AACAGGACAG GGAGAATGCT GCTCTCAAGG 
     1981 CCAGAGTGGA AGAGTTTAAC AGAAGGGTTC GGGAGAATCC TTGGGACACG CAACTATGGA 
     2041 TGGCGTTTGT GGCTTTCCAG GACGAGGTCA TGAGGAGTCC TGGCATCTAT GCCCTTGGGG 
     2101 AAGGAGAACA GGAGAAGCAC AGGAAGTCCC TGAAGCTCCT CCTGGAGAAG AAGCTGGCTG 
     2161 TGCTGGAGCG GGCCATCGAG AGCAACCCAG GCAGCGTGGA GCTGAAGCTT GCCAAGCTGC 
     2221 AGCTGTGCTC AGAGTTCTGG GAGCCCAGTG CGCTGGCCAA GGAGTGGCAG AAGCTCCTCT 
     2281 TCCTGCACCC CAACAACACA AGCCTGTGGC AGCGCTATCT CTCCTTTTGC CAGAGCCAGT 
     2341 TTGGTACCTT TTCTGTGTCC AAACTCCACA GTCTGTATGG CAAGTGCTTG AGTACCTTGT 
     2401 CTGCTGTTAA AGATGGCAGC ATGCTATCCC ACCCTGTGTT GCCGGGCACC GAGGAGGCCA 
     2461 TGTTTGGGCT CTTTCTTCAG CAGTGCCACT TTCTACGGCA GGCTGGCCAC TCAGAGAAGG 
     2521 TCATCTCTTT GTTCCAGGCC ATGGTTGACT TCACCTTCTT CAAGCCTGAC AGTGTGAAAG 
     2581 AGCTGCCTAC TAAAGTACAG GTGGAATTCT TTGAGCCCTT CTGGGACAGC GGAGAACCAC 
     2641 GCGTTGGGGA GAAGGGAGCC CGAGGCTGGC GGGCGTGGAT GCACCAACAG GAGCGAGGTG 
     2701 GCTGGGTGCT CATCACCCCA GATGAGGATG ACGAGGAGCC CGAAGAGGAG GACCAGGAGA 
     2761 TAAAAGACAA GACCCTGCCC CGGTGGCAGA TCTGGCTTGC TGTTGAGCGT TCTCGAGACC 
     2821 AGAGGCACTG GCGTCCCTGG CGCCCTGATA AGACCAAGAA GCAAACGGAG GAAGACTGTG 
     2881 AGGACCCTGA GAGACAGGTG CTGTTTGATG ACATTGGGCA ATCTTTGATC AGACTTTCCA 
     2941 GCCCAGACCT TCAGTTCCAG CTCATCCAGG CCTTTCTGCA GTTCCTGGGC GTGCCTTCCG 
     3001 GCTTCCTGCC CCCAGCCTCC TGCCTTTACC TGGCCATGGA CGAGAGCAGC ATCTTTGAGA 
     3061 GTGAACTTTA TGATGAAAAG CCCCTGACTT ACTTCAACCC TTCCTTTTCG GGCATTAGCT 
     3121 GTGTCGGCTC CATGGAACAG TTGGGTCGCC CTCGCTGGAC CAAAGGTCAC AACCGAGAGG 
     3181 GCGAGGAGTT TGTCCGCAAT GTCTTCCACC TGGTGCTGCC TTTGCTCGCA GGCAAGCAGA 
     3241 AGTCCCAGCT CAGCCTCTCC TGGTTACGGT ACGAGATTGC AAAGGTCATT TGGTGTCTAC 
     3301 ACACTAAAAA GAAGCGATTG AAGTCTCAGG GAAAGAGCTG CAAAAAACTA GCCAAGAATC 
     3361 TCCTCAAGGA GCCAGAAAAC CGCAACAACT TCTGCCTCTG GAAGCAGTAT GCACACCTGG 
     3421 AGTGGTTGCT CGGTAACACA GAGGATGCCA GAAAAGTGTT CGACACAGCA CTCAGCATGG 
     3481 CAGGCAGCAG CGAGCTGAAG GACCGTGAAC TCTGCGAGCT CAGTCTGCTC TATGCCGAGC 
     3541 TGGAGATGGA ACTGTCGCCG GACTCGAGAG GAGCCACCAC AGGCCGAGCG GTCCACATAT 
     3601 TAACACGGCT GACGGAGAGC AGTCCCTACG GGCCCTACAC GGGGCAGGTC TCGTCCACCC 
     3661 AGGTGCTGAA AGCTCGGAAG GCTTACGAGC TCGCGCTGCA GGACTGCCTG GGCCAGAGCT 
     3721 GTGCCTCCAG CCCAGCTCCT GCAGAGGCCT TGGACTGCCT GGGTAGCTTG GTCCGATGCT 
     3781 TTATGCTCTT CCAGTATTTG ACTGTGGGCA TTGATGCTGC TGTGCAGATA TACGGAAGGG 
     3841 TGTTTGCCAA GCTGAAGGGC TCTGCTCGCT TAGAAGACCC AGGGCCAGAG GACAGCACCA 
     3901 GCTCCCAGAG TTTGACCAAT GTGCTCGAGG CTGTCAGCAT GATGCACACA AGCCTGCTGC 
     3961 GATTCCACAT GAACGTTTGT GTTTACCCTC TGGCTCCACT CCGAGAGACC CTCTCCGATG 
     4021 CTTTAAAGCT GTATCCGGGC AACCAGGTTC TTTGGAGGGC TTATGTACAG ATTCAGAATA 
     4081 AGTCCCACAG TGCTAACAAG ACCAGAAGGT TCTTTGACAC AGTTACTAGG TCTGCCAAAC 
     4141 ATTTGGAGCC TTGGCTATTT GCAATCGAAG CTGAGAAGCT GAGGAAAAAG CTAGTGGAAT 
     4201 CTGTTCAGAG GGTAGGAGGC AGAGAGGTCC ATGCAACAAT CCCCGAGACT GGCCTTACGC 
     4261 ATCGCATCAG AGCTTTGTTT GAAAATGCGA TTCGGAGCGA CAAGGGCAAC CAGTGTCCCC 
     4321 TACTGTGGAG GATGTATTTG AATTTTTTGG TTTCCTTAGG AAACAAAGAA AGAAGCAAAG 
     4381 GCGTGTTCTA CAAGGCCCTC CAGAGCTGTC CTTGGGCAAA GGTGCTATAC ATGGACGCCA 
     4441 TGGAGTATTT CCCAGACGAG CTGCAGGAGA TCCTGGACGT GATGACCGAG AAGGAGCTCC 
     4501 GTGTGCGCCT GCCCCTTGAG GAGCTGGAGC TGCTGCTGGA GGATAGCGGA CCGACGCGTA 
     4561 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4621 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4681 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4741 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4801 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4861 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4921 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4981 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5041 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5101 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5161 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5221 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5281 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5341 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5401 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5461 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5521 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5581 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5641 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5701 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5761 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5821 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5881 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5941 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6001 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6061 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6121 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6181 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6241 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6301 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6361 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6421 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6481 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6541 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6601 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6661 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6721 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6781 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6841 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6901 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6961 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7021 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7081 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7141 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7201 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7261 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7321 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7381 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7441 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7501 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7561 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7621 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7861 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7921 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7981 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8041 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8101 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8161 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8221 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8281 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8341 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8401 AATTTT
//