LOCUS       MR231643                8379 bp    DNA     circular  SYN 
DEFINITION  Agap2 (myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (Agap2), transcript variant 2
ACCESSION   NM_001301014
VERSION     NM_001301014.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="Agap2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGGGGC GCGGGCGCGC TTCAGCGCCG GACAACGACC TACCTCATCT 
     1081 CGCTGACCCT GGTCAAGCTC GAGTCGGTAC CTCCGCCGCC GCCTTCTCCA TCTGCAGCCG 
     1141 CAGCCGGCGC CCCCGGGGCC AGAGGCTCCG AGCCCCGAGA TCCTGGCAGC CCCCGAGGCT 
     1201 CGGAGGAACC CGGCAAGAAG CGGCACGAGC GTCTCTTCCA CCGGCAGGAT GCGCTGTGGA 
     1261 TCAGTACCAG CAGCGCAGGC ACCGGGGGTG CGGAGCCCCC CGCGCTGTCC CCGGCTCCGG 
     1321 CCAGTCCGGC TCGCCCGGTC TCCCCGGCTC CGGGCCGCCG ACTGTCCCTA TGGGCCGCCC 
     1381 CTCCGGGACC CCCGCTCTCC GGGGGGCTGA GCCCGGACTC CAAGCCGGGA GGCGCCCCCT 
     1441 CTTCCTCCCG GCGCCCTCTA CTCAGCAGCC CGAGCTGGGG GGGTCCGGAA CCTGAAGGCC 
     1501 GGACGGGTGG CGGAGTCCCG GGCTCGTCCT CCCCGCATCC TGGCACCGGT AGCCGAAGGC 
     1561 TCAAGGTGGC GCCTCCTCCA CCTGCTCCCA AGCCTTTCAA GACCGTGACC ACTAGTGGAG 
     1621 CCAAAGCCGG CGGGGGCAAG GGCGCAGGCA GCCGCCTGTC ATGGCCCGAA AGCGAGGGCA 
     1681 AACCCAGGGT CAAGGGGTCA AAGAGCACCG CCGGGACTGG AGCTTCTGCC GCTGCGGCCG 
     1741 GCGGGGGAGG GAGCGCTGCA GTCACGACCT CTGGTGGGGT CGGGGCTGGG GCTGGAACCA 
     1801 GAGGGAAGTT GTCCCCTCGG AAAGGCAAGA GTAAGACCTT GGACAATAGT GACTTACACC 
     1861 CAGGACCCAG TGCCGGCTCT CCTCCGCTCA CCGTGCCAGC AATCCCAGTT CCAGCCACTT 
     1921 CTGTCACTGC CACCTCCACG CAGCCCCTCG GGCCTGCACC CCCTATCACT CTGGAGCCAC 
     1981 CAGCTCCAGG GCTGAAAAGG GGCCGGGAGG GCGGCCGAGC GTCCACTCGT GACCGCAAGA 
     2041 TGCTCAAGTT TATCAGCGGT ATCTTCACCA AGAGCACAGG AGGGCCTCCT GGTCCAGGGC 
     2101 CCCTTCCCGG ACCCCAAGGC TTGTCTTCCA GCAGCGGGTC CAGGGAGCTG CTGGGAGCAG 
     2161 AACTACGCAC CTCCCCTAAG GCTGTGGTTA ATAGCCAGGA ATGGACTTTG AGCCGATCAA 
     2221 TTCCCGAACT GCGCCTGGGT GTGCTGGGTG ATGTCAGGAG TGGGAAGTCG TCCCTCATCC 
     2281 ATAGATTCCT CACGGGTTCA TACCAGGTGC TGGAGAAGAT TGAGAGTGAG CAGTACAAGA 
     2341 AGGAGATGCT AGTGGATGGG CAGACCCATC TGGTGCTGAT CCGGGAGGAA GCAGGGGCAC 
     2401 CCGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC CTGGAGGATG 
     2461 AGAGCAGCTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGCTCCCTC CGGGGGGAGG 
     2521 GGCGAGGAGG TCTGGCCCTG GCTCTGGTTG GGACCCAAGA CAGGATCAGT GCATCATCTC 
     2581 CTCGAGTGGT GGGTGATGCT CGAGCCAGGG CTCTGTGCAC GGACATGAAG CGCTGTAGCT 
     2641 ACTACGAGAC CTGCGCAACC TATGGGCTCA ATGTGGACCG GGTCTTCCAG GAGGTGGCCC 
     2701 AGAAGGTGGT GACCTTGCGC AAACAACAAC AACTCCTGGC TGCTTGCAAA TCCCTGCCTA 
     2761 GCTCGCCAAG CCATTCAGCT GCATCTACTC CTGTAGCTGG GCAGGCTAGT AACGGGGGCC 
     2821 ACACTAGCGA CTACTCTTCT TCCCTCCCAT CCTCACCCAA CGTTGGACAC CGGGAGCTCC 
     2881 GAGCTGAGGC AGCTGCGGTG GCTGGGTTGA GTACCCCAGG GTCCTTACAC AGAGCAGCCA 
     2941 AGCGGCGGAC CAGCCTTTTT GCGAACCGTC GGGGCAGCGA TGCTGAGAAG CGGAGCTTAG 
     3001 ACAGCCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG AGCTTCCTGC 
     3061 TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTG ACCTTGTCCA 
     3121 GCAATGGCTT TCTACTCTAC CACCCCAGCA TTAATGACTA CATCCACAGT ACACATGGCA 
     3181 AAGAGATGGA CTTGTTACGA ACAACGGTCA AAGTCCCAGG CAAGCGGCCC CCAAGGGCTA 
     3241 TCTCTGCTTT CGGCCCCTCA GCCAGCATCA ACGGGTTGGT CAAGGATATG AGCACCGTCC 
     3301 AGATGGGTGA AGGCCCTGAA GCCTCTACTC CCATGCCAAG CCCCAGCCCT AGCCCCAGTT 
     3361 CCCTGCAGCT TCCAACAGAC CAGACATCCA AGCACCTCTT GAAGCCAGAC CGGAATTTGG 
     3421 CCCGTGCCCT TAGCACCGAC TGTACCCCAT CTGGAGATCT GAGTCCCCTG AGTCGGGAAC 
     3481 CCCCACCTTC GCCCATGGTG AAGAAGCAGA GGAGGAAGAA ACTGTCCACG CCTTCTAAGA 
     3541 CTGAAGGCTC GGCTGTGCAA GCTGAAGAGG AAAACTTTGA GTTCCTGATC GTGTCCAGCA 
     3601 CTGGTCAGAC GTGGCACTTT GAGGCAGCCA GCTTTGAGGA GCGGGATGCT TGGGTTCAGG 
     3661 CCATTGAGAG TCAGATCCTG GCCAGTCTGC AGTGCTGTGA GAGCAGCAAG GTCAAGCTGC 
     3721 GCACTGACAG CCAAAGCGAA GCTGTGGCCA TCCAGGCGAT CCGCAACGCA AAGGGAAACT 
     3781 CGACCTGTGT GGACTGTGGA GCTCCCAACC CCACGTGGGC CAGTTTGAAT CTGGGCGCAC 
     3841 TCATCTGCAT CGAGTGTTCT GGCATCCACC GGAACCTGGG CACACACCTG TCCCGAGTGC 
     3901 GTTCACTGGA TTTGGATGAC TGGCCGCGGG AGCTGACCCT GGTGCTGACG GCCATTGGCA 
     3961 ACGACACAGC CAACCGCGTG TGGGAGAGCG ACACTCGAGG CCGTGCCAAG CCTACACGGG 
     4021 ACTCTTCGCG GGAGGAGCGT GAGTCGTGGA TTCGCGCCAA GTATGAGCAG CTGCTGTTCC 
     4081 TGGCGCCTCT GGGCACCACG GAGGAGCCGC TGGGCCGCCA ACTGTGGGCC GCTGTGGAGG 
     4141 CCCAGGACGT GGCCGCCGTT CTGCTGCTTC TGGCCCACGC GCGACATGGG CCGCTCGACA 
     4201 CCAGCGTGGA GGACCCGCAG CTCCGCTCGC CGCTGCACTT GGCGGCCGAG CTCGCCCACG 
     4261 TTGTCATTAC ACAGCTGCTG TTGTGGTATG GCGCAGACGT GGCGGCCCGC GATGCGCAAG 
     4321 GCCGCACGGC ACTGTTCTAT GCCCGCCAGG CTGGCAGCCA GCTGTGTGCA GACATCCTTC 
     4381 TCCAGCACGG CTGCCCGGGA GAGGGCGGCA GCACGGCCAC CACGCCCAGC GCGGCCACCA 
     4441 CCCCCAGCAT TACCGCCACG CCCAGCCCGA GGCGCCGGAG CAGTGCTGCC AGCTTGGGCC 
     4501 GCGTGGACAC CACGATCGCG CTGGTAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//