LOCUS       MR231638                8367 bp    DNA     circular  SYN 
DEFINITION  Itgal (myc-DDK-tagged) - Mouse integrin alpha L (Itgal), transcript variant 1
ACCESSION   NM_001253872
VERSION     NM_001253872.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4524..4615 
                     /label="Myc-DDK"  
     misc_feature    4645..5230 
                     /label="PolyA signal"  
     misc_feature    4702..4721 
                     /label="Restriction Enzyme"  
     misc_feature    5506..6178 
                     /label="ColE1"  
     misc_feature    6608..7402 
                     /label="Kan/Neo"  
     misc_feature    7437..7739 
                     /label="SV40 ori"  
     misc_feature    7825..8302 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4506..4512 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4506 
                     /label="Itgal"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTCCGG ATTGCGGGCC CCAGACTTTT GCTACTGGGA CTCCAGCTGT 
     1081 TTGCCAAGGC CTGGAGCTAC AACCTGGACA CACGGCCTAC GCAGAGCTTC TTGGCACAAG 
     1141 CTGGAAGACA TTTTGGGTAC CAGGTCTTGC AGATTGAAGA TGGGGTTGTC GTGGGAGCCC 
     1201 CAGGTGAGGG GGACAACACG GGAGGCCTCT ATCACTGCCG AACAAGCAGC GAGTTCTGCC 
     1261 AGCCAGTCAG CCTACATGGT TCTAACCATA CCTCCAAGTA CTTGGGAATG ACGCTGGCAA 
     1321 CAGATGCCGC CAAGGGAAGC CTTTTGGCCT GTGACCCTGG ACTGTCTCGG ACATGCGATC 
     1381 AGAACACTTA CCTCAGTGGC CTCTGCTACC TCTTCCCCCA GAGTCTGGAG GGACCTATGT 
     1441 TACAAAATCG TCCCGCCTAT CAGGAATGTA TGAAGGGCAA AGTCGACCTG GTGTTTCTGT 
     1501 TCGATGGCTC ACAGAGCTTG GATAGAAAGG ACTTTGAAAA AATCCTGGAA TTCATGAAGG 
     1561 ATGTGATGAG GAAGCTCAGC AATACTTCCT ACCAGTTTGC TGCCGTCCAG TTCTCCACAG 
     1621 ACTGCAGAAC AGAATTTACT TTCTTGGACT ACGTTAAGCA GAACAAGAAC CCCGATGTTC 
     1681 TGCTAGGCAG CGTGCAGCCC ATGTTCTTGC TGACCAATAC CTTTCGTGCC ATCAACTATG 
     1741 TGGTGGCACA CGTGTTCAAA GAAGAGTCTG GTGCCAGGCC GGATGCTACC AAGGTGCTTG 
     1801 TCATCATTAC AGACGGGGAG GCAAGTGATA AAGGCAACAT CAGTGCGGCC CACGACATAA 
     1861 CCCGCTACAT CATCGGGATT GGCAAGCATT TTGTGAGCGT ACAAAAGCAA AAGACGCTCC 
     1921 ACATATTTGC CTCAGAACCT GTAGAGGAAT TTGTGAAGAT TCTGGACACC TTTGAGAAGC 
     1981 TGAAGGATCT TTTTACTGAC CTGCAGAGGA GGATTTATGC TATTGAGGGC ACAAACAGAC 
     2041 AGGACCTGAC ATCCTTTAAC ATGGAACTCT CCTCCAGCGG GATCAGCGCA GACCTCAGCA 
     2101 AGGGCCATGC AGTTGTGGGA GCTGTTGGGG CTAAGGATTG GGCCGGGGGC TTTCTGGACC 
     2161 TGCGTGAAGA CCTGCAGGGT GCCACATTTG TTGGGCAGGA ACCGCTGACC TCAGATGTGA 
     2221 GAGGGGGCTA CCTGGGTTAC ACTGTGGCCT GGATGACCTC CCGGAGCTCC AGACCCCTGC 
     2281 TGGCAGCAGG AGCCCCACGG TACCAGCATG TGGGACAAGT ACTGCTTTTC CAAGCCCCAG 
     2341 AGGCTGGAGG ACGTTGGAAC CAAACCCAGA AGATAGAAGG GACTCAGATC GGATCTTACT 
     2401 TTGGTGGGGA ACTATGTAGT GTTGACCTGG ACCAAGATGG CGAGGCAGAG CTGCTGCTGA 
     2461 TTGGAGCACC CCTGTTCTTT GGGGAGCAGA GAGGAGGCCG AGTGTTCACT TACCAGAGAA 
     2521 GACAGTCGCT GTTTGAAATG GTCTCAGAGC TACAGGGTGA CCCTGGCTAC CCGCTTGGTC 
     2581 GGTTTGGAGC CGCCATAACT GCCCTGACGG ACATCAATGG GGATAGGCTG ACTGATGTGG 
     2641 CTGTGGGAGC CCCTTTGGAG GAGCAGGGGG CTGTGTACAT CTTCAATGGG AAGCCTGGTG 
     2701 GGCTCAGTCC CCAGCCAAGC CAGCGTATAC AAGGAGCCCA GGTGTTCCCA GGAATCCGGT 
     2761 GGTTTGGCCG CTCCATCCAT GGGGTGAAGG ACCTTGGAGG GGACAGGCTG GCAGATGTGG 
     2821 TTGTAGGAGC TGAGGGTCGG GTGGTTGTGC TGAGCTCCAG GCCGGTGGTG GATGTGGTCA 
     2881 CTGAGCTGTC GTTCTCCCCA GAGGAAATCC CAGTGCACGA GGTGGAGTGC TCCTACTCAG 
     2941 CCAGGGAGGA GCAGAAACAC GGAGTCAAGC TCAAGGCATG CTTCCGGATC AAGCCCCTCA 
     3001 CGCCACAGTT TCAAGGTCGC CTGCTTGCCA ACCTCAGCTA CACCCTGCAG CTGGATGGCC 
     3061 ATCGGATGAG GAGCCGAGGG TTGTTCCCAG ATGGAAGCCA CGAGCTCAGT GGAAACACCT 
     3121 CCATCACCCC AGATAAATCC TGCTTGGACT TCCACTTCCA CTTCCCGATC TGCATTCAAG 
     3181 ACCTCATCTC CCCTATCAAT GTCTCCCTGA ATTTCTCTCT TTTGGAGGAA GAAGGAACAC 
     3241 CAAGGGACCA AAAGGTGGGC AGGGCCATGC AGCCTATCCT GAGACCTTCA ATCCACACAG 
     3301 TGACTAAGGA GATCCCTTTT GAGAAGAACT GTGGTGAAGA TAAGAAGTGT GAGGCAAACC 
     3361 TGACCCTGTC ATCCCCTGCC AGATCTGGAC CCCTGCGTCT GATGTCCTCT GCCAGCCTTG 
     3421 CTGTGGAGTG GACACTGAGC AACTCAGGGG AAGATGCCTA CTGGGTGCGA TTAGACCTGG 
     3481 ACTTCCCTCG GGGACTCTCC TTCCGGAAAG TGGAGATGCT TCAGCCACAC AGCCGAATGC 
     3541 CTGTGAGCTG CGAGGAGCTC ACCGAGGGGT CAAGTCTCCT GACTAAGACA CTGAAATGCA 
     3601 ATGTAAGCTC TCCCATCTTC AAAGCAGGCC AGGAGGTGAG CCTCCAGGTG ATGTTTAACA 
     3661 CGCTACTCAA CAGCTCCTGG GAAGACTTCG TTGAGCTGAA TGGCACTGTG CACTGTGAGA 
     3721 ATGAGAACTC AAGCCTCCAG GAGGACAACT CAGCCGCCAC CCACATTCCT GTCCTGTACC 
     3781 CTGTCAACAT CCTTACTAAG GAGCAGGAGA ACTCCACTCT CTATATCAGT TTCACCCCTA 
     3841 AAGGTCCCAA GACCCAACAA GTCCAGCATG TCTACCAGGT GAGGATTCAG CCATCTGCCT 
     3901 ATGACCACAA CATGCCCACA CTAGAGGCCT TGGTTGGGGT GCCCTGGCCT CACAGTGAGG 
     3961 ACCCCATCAC ATACACATGG AGTGTACAGA CGGATCCCCT TGTCACTTGC CACAGCGAGG 
     4021 ACCTGAAGAG GCCGTCCAGC GAAGCTGAGC AGCCTTGTCT GCCTGGAGTC CAGTTCCGCT 
     4081 GTCCAATTGT CTTCAGGCGG GAGATCCTCA TCCAAGTGAC GGGGACCGTG GAACTCTCCA 
     4141 AGGAAATCAA GGCCTCCTCC ACACTCAGCC TCTGCAGCTC ACTCTCCGTC TCCTTCAACA 
     4201 GCAGCAAGCA TTTCCATTTG TATGGCAGCA AAGCCTCCGA GGCCCAGGTC CTCGTGAAGG 
     4261 TTGACCTGAT CCACGAGAAG GAGATGCTTC ACGTGTACGT ACTCAGCGGC ATTGGGGGCC 
     4321 TCGTGCTTCT GTTCCTGATT TTCCTGGCGC TCTACAAGGT TGGCTTCTTC AAACGGAACC 
     4381 TGAAGGAGAA GATGGAGGCT GATGGAGGTG TTCCAAATGG AAGCCCTCCA GAAGACACTG 
     4441 ACCCTCTGGC AGTACCTGGG GAAGAGACCA AAGATATGGG CTGTCTAGAG CCCCTCCGGG 
     4501 AGAGTGACAA GGACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4561 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4621 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4681 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4741 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4801 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4861 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4921 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4981 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5041 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5101 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5161 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5221 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5281 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5341 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5401 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5461 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5521 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5581 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5641 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5701 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5761 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5821 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5881 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5941 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6001 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6061 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6121 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6181 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6241 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6301 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6361 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6421 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6481 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6541 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6601 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6661 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6721 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6781 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6841 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6901 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6961 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7021 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7081 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7141 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7201 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7261 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7321 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7381 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7441 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7501 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7561 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7741 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7801 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7861 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7921 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7981 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8041 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8101 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8161 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8221 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8281 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8341 ATTTAACAAA AATTTAACGC GAATTTT
//