LOCUS       MR231636                8364 bp    DNA     circular  SYN 
DEFINITION  Itgal (myc-DDK-tagged) - Mouse integrin alpha L (Itgal), transcript variant 3
ACCESSION   NM_001253873
VERSION     NM_001253873.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Itgal"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTCCGG ATTGCGGGCC CCAGACTTTT GCTACTGGGA CTCCAGCTGT 
     1081 TTGCCAAGGC CTGGAGCTAC AACCTGGACA CACGGCCTAC GCAGAGCTTC TTGGCACAAG 
     1141 CTGGAAGACA TTTTGGGTAC CAGGTCTTGC AGATTGAAGA TGGGGTTGTC GTGGGAGCCC 
     1201 CAGGTGAGGG GGACAACACG GGAGGCCTCT ATCACTGCCG AACAAGCAGC GAGTTCTGCC 
     1261 AGCCAGTCAG CCTACATGGT TCTAACCATA CCTCCAAGTA CTTGGGAATG ACGCTGGCAA 
     1321 CAGATGCCGC CAAGGGAAGC CTTTTGGCCT GTGACCCTGG ACTGTCTCGG ACATGCGATC 
     1381 AGAACACTTA CCTCAGTGGC CTCTGCTACC TCTTCCCCCA GAGTCTGGAG GGACCTATGT 
     1441 TACAAAATCG TCCCGCCTAT CAGGAATGTA TGAAGGGCAA AGTCGACCTG GTGTTTCTGT 
     1501 TCGATGGCTC ACAGAGCTTG GATAGAAAGG ACTTTGAAAA AATCCTGGAA TTCATGAAGG 
     1561 ATGTGATGAG GAAGCTCAGC AATACTTCCT ACCAGTTTGC TGCCGTCCAG TTCTCCACAG 
     1621 ACTGCAGAAC AGAATTTACT TTCTTGGACT ACGTTAAGCA GAACAAGAAC CCCGATGTTC 
     1681 TGCTAGGCAG CGTGCAGCCC ATGTTCTTGC TGACCAATAC CTTTCGTGCC ATCAACTATG 
     1741 TGGTGGCACA CGTGTTCAAA GAAGAGTCTG GTGCCAGGCC GGATGCTACC AAGGTGCTTG 
     1801 TCATCATTAC AGACGGGGAG GCAAGTGATA AAGGCAACAT CAGTGCGGCC CACGACATAA 
     1861 CCCGCTACAT CATCGGGATT GGCAAGCATT TTGTGAGCGT ACAAAAGCAA AAGACGCTCC 
     1921 ACATATTTGC CTCAGAACCT GTAGAGGAAT TTGTGAAGAT TCTGGACACC TTTGAGAAGC 
     1981 TGAAGGATCT TTTTACTGAC CTGCAGAGGA GGATTTATGC TATTGAGGGC ACAAACAGAC 
     2041 AGGACCTGAC ATCCTTTAAC ATGGAACTCT CCTCCAGCGG GATCAGCGCA GACCTCAGCA 
     2101 AGGGCCATGC AGTTGTGGGA GCTGTTGGGG CTAAGGATTG GGCCGGGGGC TTTCTGGACC 
     2161 TGCGTGAAGA CCTGCAGGGT GCCACATTTG TTGGGCAGGA ACCGCTGACC TCAGATGTGA 
     2221 GAGGGGGCTA CCTGGGTTAC ACTGTGGCCT GGATGACCTC CCGGAGCTCC AGACCCCTGC 
     2281 TGGCAGCAGG AGCCCCACGG TACCAGCATG TGGGACAAGT ACTGCTTTTC CAAGCCCCAG 
     2341 AGGCTGGAGG ACGTTGGAAC CAAACCCAGA AGATAGAAGG GACTCAGATC GGATCTTACT 
     2401 TTGGTGGGGA ACTATGTAGT GTTGACCTGG ACCAAGATGG CGAGGCAGAG CTGCTGCTGA 
     2461 TTGGAGCACC CCTGTTCTTT GGGGAGCAGA GAGGAGGCCG AGTGTTCACT TACCAGAGAA 
     2521 GACAGTCGCT GTTTGAAATG GTCTCAGAGC TACAGGGTGA CCCTGGCTAC CCGCTTGGTC 
     2581 GGTTTGGAGC CGCCATAACT GCCCTGACGG ACATCAATGG GGATAGGCTG ACTGATGTGG 
     2641 CTGTGGGAGC CCCTTTGGAG GAGCAGGGGG CTGTGTACAT CTTCAATGGG AAGCCTGGTG 
     2701 GGCTCAGTCC CCAGCCAAGC CAGCGTATAC AAGGAGCCCA GGTGTTCCCA GGAATCCGGT 
     2761 GGTTTGGCCG CTCCATCCAT GGGGTGAAGG ACCTTGGAGG GGACAGGCTG GCAGATGTGG 
     2821 TTGTAGGAGC TGAGGGTCGG GTGGTTGTGC TGAGCTCCAG GCCGGTGGTG GATGTGGTCA 
     2881 CTGAGCTGTC GTTCTCCCCA GAGGAAATCC CAGTGCACGA GGTGGAGTGC TCCTACTCAG 
     2941 CCAGGGAGGA GCAGAAACAC GGAGTCAAGC TCAAGGCATG CTTCCGGATC AAGCCCCTCA 
     3001 CGCCACAGTT TCAAGGTCGC CTGCTTGCCA ACCTCAGCTA CACCCTGCAG CTGGATGGCC 
     3061 ATCGGATGAG GAGCCGAGGG TTGTTCCCAG ATGGAAGCCA CGAGCTCAGT GGAAACACCT 
     3121 CCATCACCCC AGATAAATCC TGCTTGGACT TCCACTTCCA CTTCCCGATC TGCATTCAAG 
     3181 ACCTCATCTC CCCTATCAAT GTCTCCCTGA ATTTCTCTCT TTTGGAGGAA GAAGGAACAC 
     3241 CAAGGGACCA AAAGGGCAGG GCCATGCAGC CTATCCTGAG ACCTTCAATC CACACAGTGA 
     3301 CTAAGGAGAT CCCTTTTGAG AAGAACTGTG GTGAAGATAA GAAGTGTGAG GCAAACCTGA 
     3361 CCCTGTCATC CCCTGCCAGA TCTGGACCCC TGCGTCTGAT GTCCTCTGCC AGCCTTGCTG 
     3421 TGGAGTGGAC ACTGAGCAAC TCAGGGGAAG ATGCCTACTG GGTGCGATTA GACCTGGACT 
     3481 TCCCTCGGGG ACTCTCCTTC CGGAAAGTGG AGATGCTTCA GCCACACAGC CGAATGCCTG 
     3541 TGAGCTGCGA GGAGCTCACC GAGGGGTCAA GTCTCCTGAC TAAGACACTG AAATGCAATG 
     3601 TAAGCTCTCC CATCTTCAAA GCAGGCCAGG AGGTGAGCCT CCAGGTGATG TTTAACACGC 
     3661 TACTCAACAG CTCCTGGGAA GACTTCGTTG AGCTGAATGG CACTGTGCAC TGTGAGAATG 
     3721 AGAACTCAAG CCTCCAGGAG GACAACTCAG CCGCCACCCA CATTCCTGTC CTGTACCCTG 
     3781 TCAACATCCT TACTAAGGAG CAGGAGAACT CCACTCTCTA TATCAGTTTC ACCCCTAAAG 
     3841 GTCCCAAGAC CCAACAAGTC CAGCATGTCT ACCAGGTGAG GATTCAGCCA TCTGCCTATG 
     3901 ACCACAACAT GCCCACACTA GAGGCCTTGG TTGGGGTGCC CTGGCCTCAC AGTGAGGACC 
     3961 CCATCACATA CACATGGAGT GTACAGACGG ATCCCCTTGT CACTTGCCAC AGCGAGGACC 
     4021 TGAAGAGGCC GTCCAGCGAA GCTGAGCAGC CTTGTCTGCC TGGAGTCCAG TTCCGCTGTC 
     4081 CAATTGTCTT CAGGCGGGAG ATCCTCATCC AAGTGACGGG GACCGTGGAA CTCTCCAAGG 
     4141 AAATCAAGGC CTCCTCCACA CTCAGCCTCT GCAGCTCACT CTCCGTCTCC TTCAACAGCA 
     4201 GCAAGCATTT CCATTTGTAT GGCAGCAAAG CCTCCGAGGC CCAGGTCCTC GTGAAGGTTG 
     4261 ACCTGATCCA CGAGAAGGAG ATGCTTCACG TGTACGTACT CAGCGGCATT GGGGGCCTCG 
     4321 TGCTTCTGTT CCTGATTTTC CTGGCGCTCT ACAAGGTTGG CTTCTTCAAA CGGAACCTGA 
     4381 AGGAGAAGAT GGAGGCTGAT GGAGGTGTTC CAAATGGAAG CCCTCCAGAA GACACTGACC 
     4441 CTCTGGCAGT ACCTGGGGAA GAGACCAAAG ATATGGGCTG TCTAGAGCCC CTCCGGGAGA 
     4501 GTGACAAGGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//