LOCUS       MR231634                8364 bp    DNA     circular  SYN 
DEFINITION  Tsc1 (myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1), transcript variant 1
ACCESSION   NM_001289575
VERSION     NM_001289575.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Tsc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGTTA GCCAACATTG GGGAGCTGCT CTCCATGCTG GACTCCTCCA 
     1081 CACTGGGTGT GCGGGATGAC GTGACAGCCA TCTTCAAGGA GTCCCTCAAT TCTGAACGTG 
     1141 GGCCTATGCT TGTCAACACG TTGGTTGATT ATTACCTGGA AACCAATTCT CAGCCGGTAT 
     1201 TGCACATCCT GACCACCTTG CAGGAGCCAC ACGATAAGCA CCTCTTGGAC AAAATTAATG 
     1261 AGTATGTAGG CAAAGCTGCT ACCCGTTTAT CCATCCTCTC GCTGCTGGGG CATGTTGTGA 
     1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTCTGCCT TCTTTATTGA 
     1381 AATGTCTCAA GATGGACACT GATGTTGTGG TCCTCACAAC TGGTGTCTTG GTGTTGATCA 
     1441 CCATGCTCCC GATGATCCCG CAGTCAGGGA AGCAGCACCT TCTCGACTTC TTTGACATCT 
     1501 TTGGCCGTCT CTCGTCATGG TGCCTGAAGA AACCAGGCCA TGTGACAGAA GTGTACCTGG 
     1561 TCCATCTTCA TGCCAGTGTT TATGCCCTCT TTCACCGCCT TTATGGGATG TACCCATGTA 
     1621 ACTTCGTCTC CTTCCTGCGC TCTCACTACA GTATGAAGGA AAACGTGGAG ACTTTTGAAG 
     1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCACCC GGAATTAGTG ACTGGATCCA 
     1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGACATTAGA AACTCATGAT GTTGTAATAG 
     1801 AGTGTGCCAA AATCTCTCTG GACCCTACAG AAGCCTCGTA TGAAGATGGC TATTCTGTGT 
     1861 CACACCAGCT CTCTGCTTGC TTCCCTTACC GTTCAGCTGA TGTCACCACC AGCCCTTATG 
     1921 TGGACACACA GAATAGCTAT GGGGGTTCTA CTTCCACCCC TTCCTCCAGC TCTCGGCTGA 
     1981 TGTTGTTCAG TCCACCTGGG CAGCTACCTC AGAGTTTGAG TTCCCCATCA ACACGGCTGT 
     2041 TACCTGAGCC GCTGCAAGCT AGTCTCTGGA GCCCATCTGC GGTCTGTGGT ATGACCACTC 
     2101 CTCCTACGTC TCCTGGAAAT GTCCCAGCTG ATTTGTCACA TCCGTATAGT AAAGCCTTTG 
     2161 GTACCACTGC AGGTGGAAAA GGAACTCCTT CAGGAACCCC AGCGACCTCT CCTCCTCCAG 
     2221 CCCCACCTTG TCCCCAAGAT GACTGTGTGC ATGGTTCAGC AGCCCAGGCC TCAGCCACAG 
     2281 CCCCCAGGAA GGAAGAAAGA GCAGATTCCT CAAGGCCTTA CCTTCACAGA CAGTCAAACG 
     2341 ACCGAGGATT AGAGGATCCA CCTGGAAGCA AAGGTTCCGT TACTCTGAGG AATCTACCTG 
     2401 ATTTCCTAGG TGATCTGGCT TCTGAGGAAG ACAGTATCGA GAAAGATAAG GAAGAAGCTG 
     2461 CAATATCTAA AGAGCTTTCT GAGATCACTA CTGCAGAGGC GGATCCTGTA GTTCCTCGAG 
     2521 GGGGCTTTGA CTCTCCCTTC TACCGAGACA GTCTCTCTGG CTCTCAGCGG AAGACTCATT 
     2581 CGGCAGCCTC TGGGACTCAG GGCTCCAGCG TGAACCCTGA GCCTTTGCAC TCCTCCCTGG 
     2641 ACAAACATGG GCCTGACACA CCAAAGCAAG CCTTTACTCC CATAGACCCA CCCTCTGGCA 
     2701 GTGCTGATGT CAGTCCCGCT GGGGACAGGG ATCGCCAGAC TTCTCTGGAG ACCAGTATCC 
     2761 TCACTCCCAG CCCTTGCAAA ATCCCACCTC AGAGGGGAGT GAGCTTTGGA AGTGGGCAGC 
     2821 TTCCCCCATA TGATCATCTC TTTGAGGTGG CCTTGCCAAA GACTGCCTGT CACTTTGTCA 
     2881 GCAAGAAGAC TGAGGAGCTG TTGAAGAAAG TGAAAGGAAA CCCTGAGGAA GACTGTGTGC 
     2941 CCTCTACCTC CCCAATGGAA GTACTGGACA GACTGATAGA GCAGGGAGCA GGTGCGCACA 
     3001 GCAAGGAGCT GAGCAGGTTG TCCCTGCCCA GCAAGTCTGT TGACTGGACC CACTTTGGAG 
     3061 GCTCTCCTCC CTCAGATGAG CTCCGGACCC TCCGAGACCA GTTGCTTTTA CTGCACAATC 
     3121 AGCTGCTGTA CGAGCGCTTC AAGCGGCAGC AGCATGCACT CAGGAACAGA AGGCTCCTGC 
     3181 GCAAGGTGAT CCGAGCAGCG GCTCTGGAGG AACACAATGC AGCAATGAAA GATCAGTTGA 
     3241 AGTTACAAGA GAAGGACATA CAGATGTGGA AGGTGAGTCT GCAGAAAGAA CAAGCCCGAT 
     3301 ACAGTCAGCT TCAGGAACAA CGTGACACCA TGGTGACCCA ACTGCATAGC CAGATCAGAC 
     3361 AGCTACAGCA TGACCGAGAA GAATTCTACA ACCAGAGTCA GGAGTTACAG ACAAAGCTGG 
     3421 AGGACTGCAG AAACATGATT GCGGAGCTTC GGGTGGAGCT GAAGAAGGCT AACAACAAGG 
     3481 TGTGCCACAC TGAGCTGCTG CTCAGCCAGG TCTCTCAGAA GCTCTCCAAC AGTGAGTCAG 
     3541 TGCAGCAGCA GATGGAGTTC TTGAATAGGC AGCTCCTGGT GCTCGGGGAG GTCAATGAGC 
     3601 TGTACCTGGA GCAGCTGCAG AGCAAGCATC CTGACACCAC CAAGGAAGTA GAAATGATGA 
     3661 AAACTGCATA TCGGAAAGAG CTAGAGAAAA ACAGAAGCCA CCTTCTCCAG CAGAACCAAA 
     3721 GGTTGGACGC CTCACAGAGG CGAGTTTTGG AACTGGAGTC TCTTCTGGCC AAGAAAGACC 
     3781 ACCTTCTCCT AGAACAGAAG AAATATCTTG AGGATGTCAA GAGCCAGGCG AGTGGACAGC 
     3841 TGCTGGCTGC AGAGAGCAGG TATGAGGCTC AGAGAAAGAT CACCCGGGTG TTGGAACTGG 
     3901 AGATCCTAGA CTTGTATGGC AGGTTGGAAA AAGATGGCCG CCTACGGAAA CTAGAAGAGG 
     3961 ACAGAGCAGA GGCAGCAGAG GCAGCAGAAG AGAGGCTTGA CTGTTGTAGT GATGGATGCA 
     4021 CAGATTCCTT GGTAGGACAT AATGAAGAGG CTTCTGGTCA CAATGGTGAG ACCAGGACCT 
     4081 CCAGACCTGG TGGCACCCGG GCCAGCTGTG GAGGTAGAGT CACTGGAGGC AGCAGCAGCA 
     4141 GCAGCAGTGA GCTTTCCACT CCAGAGAAAC CCCCGAGCCA GAGGTTCAGC AGCCGGTGGG 
     4201 AACCTGCCCT GGGCGAGCCC TCCAGCAGCA TCCCCACCAC TGTTGGCTCA CTTCCCAGTT 
     4261 CCAAAAGCTT CCTGGGCATG AAGGCCCGGG AGCTGTTCCG TAATAAGAGC GAGAGCCAGT 
     4321 GTGATGAGGA CAGCGTGACC ATGAGTAGCA GCAGCCTTTC TGAGACCCTG AAGACAGAAC 
     4381 TGGGCAAGGA CTCGGGCACA GAAAACAAGA CTTCCCTGAG TCTAGATGCC CCACACCCAT 
     4441 CTTCCCCAAA CTCAGACAAT GTGGGGCAGC TCCACATCAT GGACTACAAT GAGACTCATC 
     4501 CTGAACACAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//