LOCUS       MR231633                8355 bp    DNA     circular  SYN 
DEFINITION  Plcb2 (myc-DDK-tagged) - Mouse phospholipase C, beta 2 (Plcb2), transcript variant 2
ACCESSION   NM_001290790
VERSION     NM_001290790.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4512..4603 
                     /label="Myc-DDK"  
     misc_feature    4633..5218 
                     /label="PolyA signal"  
     misc_feature    4690..4709 
                     /label="Restriction Enzyme"  
     misc_feature    5494..6166 
                     /label="ColE1"  
     misc_feature    6596..7390 
                     /label="Kan/Neo"  
     misc_feature    7425..7727 
                     /label="SV40 ori"  
     misc_feature    7813..8290 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4494..4500 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4494 
                     /label="Plcb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGCTC AACCCTGTTC TATTGCCCCC TAACGTGAAG GCATATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAGTGGGACG ATGAAACTTC AATAGCCTCT CCTGTTATCC 
     1141 TCCGTGTGGA TCCCAAGGGC TATTACTTAT ACTGGACATA TCAAAATCAG AGCCAGAAAC 
     1201 TCCGGGAGGT CTTCAACATG GACTTTCCAG ACAACCACTT CCTGCTGAAA ACACTCACAG 
     1261 TGGTGTCCGG CCCTGACATG GTGGACCTCA CCTTCTACAA CTTTGTCTCT TACAAGGAGA 
     1321 ACGTAGGCAA GGACTGGGCT GAGGACGTGC TAGCTCTCGC CAAACACCCG ATGACAGTCA 
     1381 ATGCTCCTCG AAGCACATTC CTGGACAAGA TCCTGGTGAA GCTTAAGATG CAGCTCAACC 
     1441 CTGAAGGGAA GATTCCCGTG AAGAATTTTT TCCAGATGTT TCCTGCTGAT CGAAAACGGG 
     1501 TGGAAGCTGC CCTCGGTGCT TGTCACCTTG CAAAAGGCAA AAATGATGCT ATCAACCCAG 
     1561 AGGACTTCCC AGAATCCGTG TACAAGAGCT TCCTCATGAG CCTCTGTCCT CGGCCAGAAA 
     1621 TCGACGAGAT CTTCACTTCC TACCACTCTA AAGCTAAGCC CTACATGACC AAGGAACACC 
     1681 TGACCAAATT CATCAATCAG AAGCAGCGAG ACCCTCGACT CAACTCCTTG CTGTTCCCAC 
     1741 CAGCCCGGCC TGAGCAAGTT CAAGTGCTCA TTGACAAGTA CGAACCCAGC GGCATCAATG 
     1801 TGCAGAGGGG CCAGCTGTCA CCAGAGGGGA TGGTCTGGTT TCTCTGTGGA CCAGAGAACA 
     1861 GTGTGCTGGC CCACGATACA CTGCTGATCC ACCAAGACAT GACACAACCA CTGAATCACT 
     1921 ATTTCATCAA CTCCTCACAC AACACCTACC TGACAGCCGG CCAGTTCTCA GGCCTTTCCT 
     1981 CGGCTGAGAT GTACCGCCAG GTGCTGCTGT CTGGCTGCCG GTGTGTAGAG TTAGACTGTT 
     2041 GGAAGGGCAA GCCCCCTGAC GAAGAGCCCA TTATCACCCA CGGCTTCACC ATGACCACAG 
     2101 ATATCTTGTT CAAGGAAGCA ATTGAAGCCA TTGCAGAAAG TGCCTTTAAG ACCTCCCCGT 
     2161 ATCCTGTCAT CCTATCATTT GAAAACCATG TAGACTCACC CCGGCAACAG GCTAAGATGG 
     2221 CTGAGTACTG CAGGAGCATG TTTGGAGAGA CCTTGCTCAC AGATCCCCTG GAAAATTTCC 
     2281 CTCTGAAACC TGGCATCCCT CTGCCCAGCC CCGAGGACCT CCGGGGCAAG ATCCTCATTA 
     2341 AGAATAAGAA GAACCAGTTT TCTGGGCCAG CATCCCCCAG CAAGAAGCCT GGTGGGGTGG 
     2401 CTGAGGGCAG CCTCCCGTCT AGTGTCCCTG TAGAAGAGGA CACGGGGTGG ACTGCTGAGG 
     2461 ACCGGACTGA GGTGGAGGAG GAGGAGGTGG TGGAAGAGGA GGAAGAGGAG GAGTCAGGAA 
     2521 ACCTGGATGA AGAAGAAATT AAGAAGATGC AGTCGGATGA GGGCACAGCA GGCCTGGAGG 
     2581 TGACAGCTTA TGAGGAAATG TCCAGCCTTG TCAATTATAT CCAGCCCACC AAGTTTATCT 
     2641 CCTTCGAGTT CTCTGCACAG AAGAACAGAA GTTACGTTGT CTCTTCCTTC ACGGAGCTCA 
     2701 AGGCCTACGA GCTGCTCTCC AAGGCCTCCA TGCAGTTTGT GGACTATAAT AAACGCCAGA 
     2761 TGAGCCGTGT GTACCCCAAG GGCACACGCA TGGACTCTTC CAACTACATG CCCCAGATGT 
     2821 TCTGGAATGC TGGATGTCAG ATGGTTGCCC TCAATTTCCA GACAATGGAC CTGCCCATGC 
     2881 AGCAGAACAT GGCACTGTTT GAGTTCAACG GGCAGAGTGG CTACCTCCTA AAGCACGAGT 
     2941 TCATGCGCAG ACTGGACAAG CAGTTCAACC CCTTCTCAGT GGACCGCATT GATGTGGTGG 
     3001 TAGCCACCAC CCTTTCCATT ACGATCATCT CTGGGCAGTT CCTGTCAGAG CGCAGTGTAC 
     3061 GCACCTATGT GGAAGTGGAA CTGTTTGGCC TCCCAGGGGA CCCCAAGAGG CGGTATCGAA 
     3121 CCAAGCTGTC ACCTACTGCT AACTCCATCA ATCCTGTCTG GAAAGAGGAA CCCTTTATCT 
     3181 TTGAGAAGAT CTTGATGCCT GAGCTGGCCT CCCTCAGGAT AGCTGTGATG GAAGAAGGCA 
     3241 GCAAATTTCT TGGACACCGC ATCATCCCCA TCAATGCCTT ACATTCTGGA TACCACCATC 
     3301 TGTGCCTGCG CAGTGAGAGC AACATGGCCC TTACCATGCC AGCTCTCTTT GTCTTCCTGG 
     3361 AGATGAAGGA CTACATACCT GACACCTGGG CAGATCTTAC AGTAGCCCTT GCCAACCCCA 
     3421 TCAAGTACTT CAATGCCCAG GATAAGAAGT CCGTGAAGCT CAAAGGAGTG ACAGGAAGTC 
     3481 TGCCCGAGAA ACTCTTCTCT GGGACACCTG TTGCCAGTCA GTCCAATGGG GCGCCAGTCT 
     3541 CGGCAGGCAA TGGGTCAACA GCGCCTGGGA CCAAGGCCAC GGGGGAAGAA GCTACGAAAG 
     3601 AAGTGACAGA GCCACAGACC GCCAGCTTGG AAGAGTTGCG GGAACTGAAG GGCGTGGTGA 
     3661 AACTGCAGCG AAGACATGAG AAGGAGCTTC GAGAGTTGGA GCGCCGTGGA GCCCGGCGCT 
     3721 GGGAGGAACT GCTGCAGCGT GGCGCCGCAC AGCTGGCGGA GCTCCAGACC CAGGCAGCAG 
     3781 GCTGCAAGCT CCGCCCAGGC AAGGGCTCCC GCAAGAAGAG GACCCTGCCC TGTGAGGAGA 
     3841 CCGTCGTGGC GCCTAGCGAG CCCCACGACC GGGCGGACCC GCGCGTGCAG GAGCTGAAGG 
     3901 ACAGGCTGGA GCAGGAGCTG CAGCAGCAAG GCGAGGAGCA GTACCGCTCC GTCCTCAAGC 
     3961 GCAAGGAGCA GCACGTGACC GAGCAAATCG CCAAGATGAT GGAGCTGGCC AGAGAGAAAC 
     4021 AGGCTGCTGA ACTCAAGACC TTCAAGGAGA CCTCAGAAAC TGACACCAAA GAAATGAAGA 
     4081 AAAAACTGGA GGCCAAGAGG CTGGAACGGA TCCAAGCCAT GACCAAGGTC ACCACAGACA 
     4141 AGGTGGCCCA GGAGAGGCTC AAGAGAGAAA TTAACAACTC CCACATCCAG GAAGTGGTCC 
     4201 AGGCTGTCAA GCAGATGACA GAGACCCTGG AGCGTCACCA GGAGAAGCTG GAAGAGAGGC 
     4261 AGACAGCCTG CCTGGAGCAG ATCCAGGCAA TGGAAAAGCA GTTCCAGGAG AAGGCGCTGG 
     4321 CAGAGTATGA GGCCAAGATG AAGGGCCTGG AGGCTGAAGT AAAGGAGTCG GTGCGGGCCT 
     4381 ACTTCAAGGA CTGCTTCCCC ACCGAAGCAG AAGACAAGCC TGAGAGGTCC TGTGAGGCCT 
     4441 CTGAGGAGTC GTGTCCACAG GAACCACTTG TGAGCAAGGC AGACACTCAG GAGAGCCGCC 
     4501 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4561 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4621 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4681 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4741 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4801 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4861 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4921 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4981 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5041 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5101 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5161 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5221 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5281 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5341 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5401 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5461 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5521 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5581 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5641 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5761 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5821 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5881 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5941 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6001 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6061 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6121 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6181 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6241 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6301 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6361 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6421 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6481 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6541 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6601 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6661 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6721 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6781 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6841 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6901 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6961 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7021 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7081 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7141 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7201 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7261 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7321 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7381 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7441 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7501 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7561 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7801 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7861 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7921 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7981 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8041 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8101 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8161 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8221 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8281 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8341 TTTAACGCGA ATTTT
//