LOCUS       MR231630                8346 bp    DNA     circular  SYN 
DEFINITION  Tsc1 (myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1), transcript variant 3
ACCESSION   NM_001289576
VERSION     NM_001289576.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4503..4594 
                     /label="Myc-DDK"  
     misc_feature    4624..5209 
                     /label="PolyA signal"  
     misc_feature    4681..4700 
                     /label="Restriction Enzyme"  
     misc_feature    5485..6157 
                     /label="ColE1"  
     misc_feature    6587..7381 
                     /label="Kan/Neo"  
     misc_feature    7416..7718 
                     /label="SV40 ori"  
     misc_feature    7804..8281 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4485..4491 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4485 
                     /label="Tsc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGTTA GCCAACATTG GGGAGCTGCT CTCCATGCTG GACTCCTCCA 
     1081 CACTGGGTGT GCGGGATGAC GTGACAGCCA TCTTCAAGGA GTCCCTCAAT TCTGAACGTG 
     1141 GGCCTATGCT TGTCAACACG TTGGTTGATT ATTACCTGGA AACCAATTCT CAGCCGGTAT 
     1201 TGCACATCCT GACCACCTTG CAGGAGCCAC ACGATAAGCA CCTCTTGGAC AAAATTAATG 
     1261 AGTATGTAGG CAAAGCTGCT ACCCGTTTAT CCATCCTCTC GCTGCTGGGG CATGTTGTGA 
     1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTCTGCCT TCTTTATTGA 
     1381 AATGTCTCAA GATGGACACT GATGTTGTGG TCCTCACAAC TGGTGTCTTG GTGTTGATCA 
     1441 CCATGCTCCC GATGATCCCG CAGTCAGGGA AGCAGCACCT TCTCGACTTC TTTGACATCT 
     1501 TTGGCCGTCT CTCGTCATGG TGCCTGAAGA AACCAGGCCA TGTGACAGAA GTGTACCTGG 
     1561 TCCATCTTCA TGCCAGTGTT TATGCCCTCT TTCACCGCCT TTATGGGATG TACCCATGTA 
     1621 ACTTCGTCTC CTTCCTGCGC TCTCACTACA GTATGAAGGA AAACGTGGAG ACTTTTGAAG 
     1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCACCC GGAATTAGTG ACTGGATCCA 
     1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGACATTAGA AACTCATGAT GTTGTAATAG 
     1801 AGTGTGCCAA AATCTCTCTG GACCCTACAG AAGCCTCGTA TGAAGATGGC TATTCTGTGT 
     1861 CACACCAGCT CTCTGCTTGC TTCCCTTACC GTTCAGCTGA TGTCACCACC AGCCCTTATG 
     1921 TGGACACACA GAATAGCTAT GGGGGTTCTA CTTCCACCCC TTCCTCCAGC TCTCGGCTGA 
     1981 TGTTGTTCAG TCCACCTGGG CAGCTACCTC AGAGTTTGAG TTCCCCATCA ACACGGCTGT 
     2041 TACCTGAGCC GCTGCAAGCT AGTCTCTGGA GCCCATCTGC GGTCTGTGGT ATGACCACTC 
     2101 CTCCTACGTC TCCTGGAAAT GTCCCAGCTG ATTTGTCACA TCCGTATAGT AAAGCCTTTG 
     2161 GTACCACTGG TGGAAAAGGA ACTCCTTCAG GAACCCCAGC GACCTCTCCT CCTCCAGCCC 
     2221 CACCTTGTCC CCAAGATGAC TGTGTGCATG GTTCAGCAGC CCAGGCCTCA GCCACAGCCC 
     2281 CCAGGAAGGA AGAAAGAGCA GATTCCTCAA GGCCTTACCT TCACAGACAG TCAAACGACC 
     2341 GAGGATTAGA GGATCCACCT GGAAGCAAAG GTTCCGTTAC TCTGAGGAAT CTACCTGATT 
     2401 TCCTAGGTGA TCTGGCTTCT GAGGAAGACA GTATCGAGAA AGATAAGGAA GAAGCTGCAA 
     2461 TATCTAAAGA GCTTTCTGAG ATCACTACTG CAGAGGCGGA TCCTGTAGTT CCTCGAGGGG 
     2521 GCTTTGACTC TCCCTTCTAC CGAGACAGTC TCTCTGGCTC TCAGCGGAAG ACTCATTCGG 
     2581 CAGCCTCTGG GACTCAGGGC TCCAGCGTGA ACCCTGAGCC TTTGCACTCC TCCCTGGACA 
     2641 AACATGGGCC TGACACACCA AAGCAAGCCT TTACTCCCAT AGACCCACCC TCTGGCAGTG 
     2701 CTGATGTCAG TCCCGCTGGG GACAGGGATC GCCAGACTTC TCTGGAGACC AGTATCCTCA 
     2761 CTCCCAGCCC TTGCAAAATC CCACCTCAGA GGGGAGTGAG CTTTGGAAGT GGGCAGCTTC 
     2821 CCCCATATGA TCATCTCTTT GAGGTGGCCT TGCCAAAGAC TGCCTGTCAC TTTGTCAGCA 
     2881 AGAAGACTGA GGAGCTGTTG AAGAAAGTGA AAGGAAACCC TGAGGAAGAC TGTGTGCCCT 
     2941 CTACCTCCCC AATGGAAGTA CTGGACAGAC TGATAGAGCA GGGAGCAGGT GCGCACAGCA 
     3001 AGGAGCTGAG CAGGTTGTCC CTGCCCAGCA AGTCTGTTGA CTGGACCCAC TTTGGAGATG 
     3061 AGCTCCGGAC CCTCCGAGAC CAGTTGCTTT TACTGCACAA TCAGCTGCTG TACGAGCGCT 
     3121 TCAAGCGGCA GCAGCATGCA CTCAGGAACA GAAGGCTCCT GCGCAAGGTG ATCCGAGCAG 
     3181 CGGCTCTGGA GGAACACAAT GCAGCAATGA AAGATCAGTT GAAGTTACAA GAGAAGGACA 
     3241 TACAGATGTG GAAGGTGAGT CTGCAGAAAG AACAAGCCCG ATACAGTCAG CTTCAGGAAC 
     3301 AACGTGACAC CATGGTGACC CAACTGCATA GCCAGATCAG ACAGCTACAG CATGACCGAG 
     3361 AAGAATTCTA CAACCAGAGT CAGGAGTTAC AGACAAAGCT GGAGGACTGC AGAAACATGA 
     3421 TTGCGGAGCT TCGGGTGGAG CTGAAGAAGG CTAACAACAA GGTGTGCCAC ACTGAGCTGC 
     3481 TGCTCAGCCA GGTCTCTCAG AAGCTCTCCA ACAGTGAGTC AGTGCAGCAG CAGATGGAGT 
     3541 TCTTGAATAG GCAGCTCCTG GTGCTCGGGG AGGTCAATGA GCTGTACCTG GAGCAGCTGC 
     3601 AGAGCAAGCA TCCTGACACC ACCAAGGAAG TAGAAATGAT GAAAACTGCA TATCGGAAAG 
     3661 AGCTAGAGAA AAACAGAAGC CACCTTCTCC AGCAGAACCA AAGGTTGGAC GCCTCACAGA 
     3721 GGCGAGTTTT GGAACTGGAG TCTCTTCTGG CCAAGAAAGA CCACCTTCTC CTAGAACAGA 
     3781 AGAAATATCT TGAGGATGTC AAGAGCCAGG CGAGTGGACA GCTGCTGGCT GCAGAGAGCA 
     3841 GGTATGAGGC TCAGAGAAAG ATCACCCGGG TGTTGGAACT GGAGATCCTA GACTTGTATG 
     3901 GCAGGTTGGA AAAAGATGGC CGCCTACGGA AACTAGAAGA GGACAGAGCA GAGGCAGCAG 
     3961 AGGCAGCAGA AGAGAGGCTT GACTGTTGTA GTGATGGATG CACAGATTCC TTGGTAGGAC 
     4021 ATAATGAAGA GGCTTCTGGT CACAATGGTG AGACCAGGAC CTCCAGACCT GGTGGCACCC 
     4081 GGGCCAGCTG TGGAGGTAGA GTCACTGGAG GCAGCAGCAG CAGCAGCAGT GAGCTTTCCA 
     4141 CTCCAGAGAA ACCCCCGAGC CAGAGGTTCA GCAGCCGGTG GGAACCTGCC CTGGGCGAGC 
     4201 CCTCCAGCAG CATCCCCACC ACTGTTGGCT CACTTCCCAG TTCCAAAAGC TTCCTGGGCA 
     4261 TGAAGGCCCG GGAGCTGTTC CGTAATAAGA GCGAGAGCCA GTGTGATGAG GACAGCGTGA 
     4321 CCATGAGTAG CAGCAGCCTT TCTGAGACCC TGAAGACAGA ACTGGGCAAG GACTCGGGCA 
     4381 CAGAAAACAA GACTTCCCTG AGTCTAGATG CCCCACACCC ATCTTCCCCA AACTCAGACA 
     4441 ATGTGGGGCA GCTCCACATC ATGGACTACA ATGAGACTCA TCCTGAACAC AGCACGCGTA 
     4501 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4561 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5281 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5341 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5401 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5461 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5521 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5581 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5641 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5701 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5761 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5821 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5881 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5941 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6001 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6061 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6121 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6181 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6241 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6301 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6361 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6421 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6481 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6541 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6601 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6661 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6721 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6781 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6841 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6901 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6961 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7021 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7081 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7141 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7201 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7261 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7321 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7381 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7801 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7861 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7921 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7981 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8041 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8101 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8161 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8221 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8281 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8341 AATTTT
//