LOCUS       MR231619                8313 bp    DNA     circular  SYN 
DEFINITION  Gak (myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak), transcript variant 2
ACCESSION   NM_001282051
VERSION     NM_001282051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="Gak"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACAGGCAG AAACCACCCA TCATCCACAG AGATCTCAAG GTTGAAAACT 
     1081 TACTGCTTAG TAACCAGGGG ACCATTAAGC TGTGTGACTT TGGCAGTGCC ACAACCATCT 
     1141 CCCATTATCC TGACTACAGC TGGAGTGCCC AGAAGCGAGC CATGGTGGAG GAAGAGATCA 
     1201 CAAGGAACAC CACACCCATG TACAGAACAC CAGAAATTGT AGACCTGTAT TCCAACTTCC 
     1261 CCATTGGCGA AAAGCAGGAT ATCTGGGCAC TGGGCTGTAT CTTATACCTG CTGTGTTTCC 
     1321 GGCAGCATCC TTTTGAGGAT GGAGCAAAAC TTCGGATAGT CAATGGGAAG TATTCCATTC 
     1381 CTGTGAATGA CACTCGTTAC ACAGTCTTCC ATGACCTTAT TCGTGCCATG CTAAAGGTCA 
     1441 ACCCAGAGGA GAGGCTGTCC ATTGCTGAAG TGGTCCGACA ACTGCAGGAA ATTGCAGCAG 
     1501 CTAGGAATGT GAACCCCAAA GCTCCCATCA CAGAGCTGCT GGAGCAGAAT GGTGGCTATG 
     1561 GGAACTCAGG GCCTTCCCGA GCACAACCAC CTTGCGGGGG CACCGTGAAC AGCAGTGGAG 
     1621 TTTTGGCTCT GGCAGAGTAT GACCAGCCCT ACGGTGGGTT TCTTGATATT CTGCGGGGTG 
     1681 GGACCGAACG GCTCTTCACC AACCTCAAGG ATACTTCCTC CAAGGTCATC CAGTCTGTGG 
     1741 CTAACTATGC AAAGGGTGAT CTGGACATAT CTTACATCAC ATCCAGGATT GCAGTGATGT 
     1801 CATTCCCAGC AGAAGGTGTG GAGTCTGCAA TCAAAAACAA TATAGAGGAT GTGCGAATGT 
     1861 TTCTGGATGC TAAGCATCCA GGACATTATG CTGTCTACAA CCTTTCTCCA AGGATATACC 
     1921 GGGCTTCCAA GTTTCACAAC CGGGTCACTG AGTGTGGCTG GGCAGTCAGA CGGGCACCAC 
     1981 ATCTCCACAG TTTGTATACT CTATGTAGGA GCATGCATGC CTGGCTCCGG GAAGACCACA 
     2041 GGAACGTCTG TGTTGTGCAT TGCATGGATG GGAGAGCTGC ATCTGCTGTG GCAGTCTGTG 
     2101 CATTCCTGTG CTTCTGCCGT CTCTTCAGCA CTGCAGAGGC TGCTGTGTAC ATGTTCAGCA 
     2161 TGAAGCGCTG CCCACCAGGC ATTTGGCCAT CCCACAAAAG GTACATTGAA TATGTATGTG 
     2221 ACATGGTGGC AGAGGAGCCC ATCACGCCCC ATAGCAAGCC AATGCTGGTG AAATCTGTTG 
     2281 TCATGACCCC CGTGCCACTG TTCAGCAAGC AGAGGAATGG CTGCCGACCG TTCTGTGAGG 
     2341 TCTACGTTGG AGAGGAGCGT GTCACCACCA CGTCGCAGGA ATATGACAGA ATGAAGGAAT 
     2401 TTAAAATTGA GGATGGCAAG GCTGTCATCC CCCTGGGCGT AACAGTTCAA GGAGACGTGC 
     2461 TCATCATCAT CTACCATGCC AGGGCCACAC TGGGAGGGAG GCTGCAGGCT AAGATGGCGT 
     2521 CCATGAAAAT GTTCCAGATC CAGTTCCACA CTGGGTTCGT GCCTCGAAAC GCAACCACTG 
     2581 TGAAATTTGC AAAATATGAC CTGGATGCTT GTGATATTCA AGAGAAGTAC CCAGATCTGT 
     2641 TCCAGGTGAA CCTGGAAGTG GAGGTAGAGC CTAGAGACAG GCCCAGCCGA GAAGCTCCAC 
     2701 CTTGGGAGAA CACCAGCCTA AGGGGGTTAA ACCCCAAGAT CCTCTTTTCC AACAGGGAAG 
     2761 AGCAGCAGGA CATTCTGTCT AAGTTTGGGA AGCCGGAGCT ACCCCGGCAG CCGGGCTCCA 
     2821 CAGCTCAGTA TGATGCTGAG GCAGGGTCTC CGGAGGCTGA GATCACAGAG TCGGACTCAC 
     2881 CGCAGAGCAG CAGTACGGAC ACCAACCACT TTCTTCACAC ACTGGATTGG CAGGAGGAAA 
     2941 AAGAACCAGA GACTGGGTTA GACAATACCT CTCCTAAGGA GAGTCAGTCT GTCCTGATTG 
     3001 CAGACGGAGA TGGAAGTGAA GTATCAGATG AAGAAGAGGC TTCATTCCCC AGTGAGGAGA 
     3061 GGAAACCAGG AGCTGGAGAA GATACACCAA GGCTGGCTGC TGGGACCAAA CAGCAAGACT 
     3121 TAATATTTGA TGTGGGCATG CTGGCTGCCC CACAGGAGCC TGTACAGCCC GAAGAAGGTG 
     3181 TTGATCTCCT GGGGCTACAC TCTGAGGGGG ACTTAAGGCC TGCTGCCCCC TTACAGGCTT 
     3241 GTGGGGTCCC ATCTAGCAAC ACTGACCTGT TGAGCTGCCT TCTTGAACCA TCTGATGCTG 
     3301 CTCAAGTAGG GCCTCCTGGT GACCTGCTTG GTGGTGAGGC TCCACTGCTG CTAGCAAGCC 
     3361 CAGTTTCTCC TCTTGGGCTG CAGAACAACC TACAAGGAAA AGTCCCTGAC ACTGTGGACC 
     3421 CATTTGACCA GTTCCTGCTG TCATCCAACT CAGACACCCA GCCCTGCTCC AAGCCTGATC 
     3481 TCTTTGGAGA GTTTCTCAAC TCTGACTCTG TAGCTTCCTC AACTGCCTTC CCATCCACCC 
     3541 ACAGTGCCCC ACCCCCATCC TGTAGCACTG CCTTCCTGCA CCTAGGGGAT CTGCCAGCAG 
     3601 AGCCCAGCAA GGTAATAGCT TCATCCAGCC ACCCAGATCT GCTAGGAGGA TGGGATACGT 
     3661 GGGCTGACAC AGCTACACCT GGGCCAGCCT CCATTCCAGT ACCAGAAGGT ACCCTCTTCT 
     3721 CTTCTGCAGG TCACCCAGCC CCTCCTGGCC CCAACCCCAG CCAAACCAAG TCTCAGAACC 
     3781 TTGACCCATT TGCTGATCTC AGTGATCTCA GCTCTAGCCT CCAAGGCCTG CCTGCTGGAC 
     3841 TTCCTGCAGG GGGCTTTGTT GGCGCACCAG CCCCCACTCA AAAGAGCAAC AGCCCCTGGC 
     3901 AGGCAAATCG TCCCACAGCC CCTGGAACCT CATGGACCCC CCAGGCCAAG CCAGCCCCCA 
     3961 GAGCCTCTGA ACAGCTAAGG TCTCACTTCA GTGTGATCGG GGCCCGAGAA GAGAGGGGCG 
     4021 TCCGTGTGCC CAGCTTTGCC CAAAAGCCAA AGGTCTCAGA AAATGATTTT GAAGATCTTC 
     4081 TGCCTAATCA AGGCTTCTCT AAGTCTGACA AGAAGGGGCC AAAGACCATG GCAGAGATGC 
     4141 GGAAACAGGA ACTTGCCAGA GATACAGACC CACTCAAATT GAAGCTTTTG GACTGGATTG 
     4201 AAGGCAAGGA GAGGAACATT CGTGCACTGC TGTCCACTCT GCACACGGTA TTGTGGGATG 
     4261 GGGAGAGCCG CTGGACACCT GTGAGTATGG CTGACCTGGT GACTCCAGAG CAGGTGAAGA 
     4321 AGCAGTACCG CCGTGCAGTG CTGGTAGTGC ATCCTGATAA GGCCACAGGG CAGCCGTATG 
     4381 AACAGTATGC CAAGATGATC TTCATGGAGC TGAATGATGC ATGGTCTGAG TTTGAAAACC 
     4441 AGGGCTCGAG GCCCCTCTTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//