LOCUS       MR231616                8289 bp    DNA     circular  SYN 
DEFINITION  Bag6 (myc-DDK-tagged) - Mouse BCL2-associated athanogene 6 (Bag6), transcript variant 2
ACCESSION   NM_001252468
VERSION     NM_001252468.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4446..4537 
                     /label="Myc-DDK"  
     misc_feature    4567..5152 
                     /label="PolyA signal"  
     misc_feature    4624..4643 
                     /label="Restriction Enzyme"  
     misc_feature    5428..6100 
                     /label="ColE1"  
     misc_feature    6530..7324 
                     /label="Kan/Neo"  
     misc_feature    7359..7661 
                     /label="SV40 ori"  
     misc_feature    7747..8224 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4428..4434 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4428 
                     /label="Bag6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGAGT GATAGTGCCA GTACCGCTAT GGAGGAGCCT GACAGCCTGG 
     1081 AGGTACTGGT GAAGACCCTG GACTCTCAGA CTCGGACTTT TATTGTGGGG GCCCAGATGA 
     1141 ATGTAAAGGA GTTTAAGGAA CACATAGCTG CCTCTGTCAG CATCCCTTCC GAGAAACAGC 
     1201 GGCTCATCTA CCAGGGCCGG GTCCTACAAG ACGACAAGAA GCTCCAGGAG TACAACGTTG 
     1261 GGGGAAAGGT TATTCACCTG GTGGAACGGG CTCCTCCTCA GACTCAGCTT CCTTCTGGAG 
     1321 CATCTTCTGG GACAGGCTCT GCCTCAGCAA CTCATGGTGG GGCACCCCTG CCTGGCACTC 
     1381 GGGGCCCTGG GGCTTCTGTT CATGACCGGA ATGCCAACAG CTATGTCATG GTTGGAACCT 
     1441 TCAATCTTCC TAGTGAGCCC CGGGTCCGGC TGGTGATGGC TCAGCACATG ATTAGGGATA 
     1501 TACAGACCTT ACTGTCCCGG ATGGAGTGTC GAGGGGGAAC CCAAGCTCAG GCCAGTCAGC 
     1561 CACCCCCGCA GACACCGCAG ACTGTGGCCT CAGAGACGGT AGCCTTGAAC TCACAAACAT 
     1621 CAGAACCAGT CGAAAGTGAA GCTCCTCCTC GAGAGCCCAT GGAGTCAGAA GAAATGGAGG 
     1681 AACGTCCCCC AACCCAGACT CCAGAGCTTG CGCCCTCAGG CCCAGCTCCC GCAGGCCCAG 
     1741 CTCCCGCAGG CCCAGCTCCC GCGCCAGAGA CAAATGCACC CAACCACCCT TCCCCCGCAG 
     1801 AGCACGTGGA GGTGCTCCAG GAGCTTCAGC GCTTGCAGCG CCGTCTTCAG CCTTTCCTAC 
     1861 AGCGCTACTG CGAGGTCCTG GGCGCTGCTG CCACCACAGA CTACAACAAC AACCATGAGG 
     1921 GCCGTGAGGA GGACCAGAGG TTGATCAACT TGGTTGGGGA GAGCCTGCGG CTACTGGGCA 
     1981 ACACTTTCGT GGCATTGTCT GATCTGCGCT GCAATCTAGC CTGTGCACCC CCACGGCACC 
     2041 TGCACGTGGT TCGGCCTATG TCTCACTACA CGACTCCCAT GGTGCTCCAG CAGGCAGCCA 
     2101 TTCCCATTCA GATCAATGTG GGGACTACTG TGACCATGAC AGGCAATGGG GCTCGGCCTC 
     2161 CACCAGCTCC TGGTGCGGAG GCAGCAACCC CAGGTTCTGC CCAGGCCACA TCCCTGCCTC 
     2221 CCTCTTCCAC CACTGTTGAT TCATCAACTG AAGGAGCTCC CCCACCAGGG CCAGCACCGC 
     2281 CACCAGCCTC CAGCCACCCA CGGGTCATCC GGATTTCCCA CCAGAGTGTG GAGCCCGTGG 
     2341 TCATGATGCA CATGAACATT CAAGATTCTG GAGCACAGCC TGGTGGTGTG CCAAGTGCTC 
     2401 CCACTGGTCC ACTGGGACCT CCTGGTCATG GACAGACCCT GGGACAGCAG GTGCCCGGCT 
     2461 TCCCAACAGC ACCAACTCGG GTGGTGATTG CCCGGCCAAC TCCTCCACAG GCTCGGCCTT 
     2521 CCCATCCTGG GGGACCTCCA GTCTCTGGAG CTCTGCAGGG CGCTGGGCTG GGTACAAACA 
     2581 CTTCATTGGC CCAGATGGTG AGCGGCCTTG TGGGGCAACT TCTTATGCAG CCTGTTCTTG 
     2641 TGGCTCAGGG GACTCCAGGA ATGGCTCAGG CTCAGGCCCA GGCCCAGGCT CAGGCCCAGG 
     2701 CCCAGGCCCA GGCTCCAGCT CCGGCTCCGG CTCCAGCTCC AGCTCCTGCC ACTGCTTCAG 
     2761 CTAGTGCTGG TACTACCAAC ACAGCTACCA CAGCTGGCCC TGCTCCTGGG GGTCCTGCCC 
     2821 AGCCTCCACC TCCTCAGCCC TCTGCAGCTG ATCTTCAGTT CTCTCAGCTC CTGGGAAATC 
     2881 TGCTGGGGCC TGCAGGGCCA GGGGCTGGGG GGCCAGGCAT GGCTTCTCCC ACCATCACTG 
     2941 TGGCAATGCC TGGTGTCCCT GCTTTTCTCC AGGGCATGAC TGATTTTTTG CAGGCATCAC 
     3001 AGACTGCCCC TCCACCCCCT CCACCTCCTC CACCCCCACC CCCTGCCCCA GAGCAGCAGA 
     3061 GCACACCCCC ACCAGGGTCT CCTTCTGGTG GAACAGCAAG CCCTGGAGGC TTAGGTCCTG 
     3121 AGAGCCTGCC ACCAGAGTTT TTCACCTCAG TGGTGCAGGG CGTGTTGAGC TCCCTCCTGG 
     3181 GCTCCTTGGG GGCTCGAGCT GGCAGCAGTG AGAGTATCGC TGCCTTCATC CAACGCCTCA 
     3241 GTGGATCCAG CAACATCTTT GAGCCTGGGG CTGATGGCGC CCTTGGATTC TTCGGAGCTC 
     3301 TGCTCTCTCT CCTGTGCCAG AATTTCTCCA TGGTGGATGT GGTGATGCTT CTCCATGGCC 
     3361 ATTTCCAGCC ACTGCAGCGG CTCCAGCCAC AGCTGCGATC TTTCTTCCAC CAGCACTACC 
     3421 TGGGTGGCCA GGAGCCCACG CCTAGCAACA TCCGGATGGC GACCCACACA CTGATCACTG 
     3481 GGCTGGAAGA GTATGTAAGG GAGAGTTTTT CTTTGGTACA GGTTCAGCCA GGTGTGGATA 
     3541 TCATCCGGAC AAATTTAGAA TTTCTCCAAG AGCAGTTTAA CAGCATTGCT GCTCATGTGC 
     3601 TGCACTGTAC AGACAGTGGA TTTGGAGCCC GGTTGCTGGA GCTGTGTAAC CAGGGCCTGT 
     3661 TTGAATGCTT GGCCCTGAAC CTCCACTGCT TGGGGGGACA GCAAATGGAG CTTGCTGCTG 
     3721 TCATCAATGG TCGAATTCGC CGCATGTCTC GCGGGGTGAA TCCATCCTTG GTGAGCTGGC 
     3781 TGACAACCAT GATGGGACTG AGGCTTCAGG TGGTCTTGGA GCACATGCCT GTGGGTCCCG 
     3841 ACGCCATCCT CAGATATGTT CGTAGGGTTG GTGATCCTCC TCAGACACTT CCTGAAGAGC 
     3901 CGATGGAAGT TCAGGGAGCA GAAAGAACTT CCCCTGAACC TCAGAGAGAG AATGCTTCCC 
     3961 CAGCCCCTGG AACAACAGCA GAAGAAGCCA TGTCCCGAGG CCCGCCCCCT GCTCCTGAAG 
     4021 GAGGTTCCCG AGATGAACAG GATGGAGCTT CAGCTGATGC AGAACCCTGG GCAGCTGCAG 
     4081 TCCCCCCTGA ATGGGTCCCT ATTATCCAGC AGGACATTCA GAGCCAGCGG AAGGTGAAAC 
     4141 CTCAGCCGCC CCTGAGTGAT GCCTACCTCA GTGGCATGCC TGCCAAGAGA CGAAAGACAA 
     4201 TGCAGGGTGA GGGCCCCCAG CTGCTACTCT CAGAGGCAGT GAGCCGGGCA GCTAAGGCAG 
     4261 CCGGAGCTCG GCCCCTGACA AGCCCCGAGA GCCTGAGCCG GGACCTGGAG GCACCAGAGG 
     4321 TTCAGGAGAG CTACAGGCAG CAGCTCCGGT CTGATATCCA AAAACGACTG CAGGAAGATC 
     4381 CCAACTACAG CCCCCAGCGC TTCCCTAATG CCCATCGGGC ATTTGCTGAT GACCCCACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//