LOCUS       MR231612                8283 bp    DNA     circular  SYN 
DEFINITION  Nckap1 (myc-DDK-tagged) - Mouse NCK-associated protein 1 (Nckap1), transcript variant 1
ACCESSION   NM_001290745
VERSION     NM_001290745.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="Nckap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCGCTCC GTGCTGCAGC CCAGTCAGCA GAAGCTGGCG GAGAAGCTCA 
     1081 CCATCCTCAA CGACCGGGGC GTCGGCATGC TCACGCGCCT CTACAACATC AAGAAGCAAG 
     1141 GACAAGTTTG GAAGGCATGT GGCGACCCCA AGGCTAAACC CTCCTACCTT ATTGACAAAA 
     1201 ACCTGGAATC TGCTGTGAAA TTCATAGTCA GAAAATTTCC TGCTGTAGAA ACGCGCAACA 
     1261 ACAATCAACA GCTGGCACAG CTACAGAAAG AAAAATCAGA GATTCTGAAA AATCTGGCGT 
     1321 TGTATTACTT TACATTTGTA GATGTTATGG AATTTAAGGA CCATGTCTGT GACTTGCTGA 
     1381 ATACTATTGA CGTTTGCCAA GTCTTCTTTG ATATTACTGT GAACTTTGAT TTAACAAAGA 
     1441 ACTACTTAGA CTTGACTGTA ACCTATACAA CATTAATGAT ACTGCTGTCC CGGATTGAAG 
     1501 AACGGAAGGC AATCATTGGA CTATACAATT ATGCACATGA AATGACCCAT GGAGCAAGTG 
     1561 ACCGAGAATA TCCACGCCTT GGTCAGATGA TTGTGGACTA TGAAAACCCT TTAAAAAAGA 
     1621 TGATGGAAGA ATTTGTACCC CATAGCAAGT CTCTTTCAGA TGCGCTAATT TCTCTTCAGA 
     1681 TGGTGTATCC TAGACGGAAC CTTTCAGCAG ACCAGTGGAG GAATGCCCAG TTGCTGAGTC 
     1741 TCATCAGTGC ACCCAGCACA ATGCTTAATC CTGCGCAGTC GGACACTATG CCTTGTGAAT 
     1801 ATCTCTCCTT GGATGCAATG GAGAAATGGA TCATCTTTGG CTTTATTCTG TGCCATGGGA 
     1861 TGCTAAATAC GGAGGCTACC GCACTGAACC TTTGGAAGCT AGCTCTTCAG AGTAGCTCTT 
     1921 GCCTCTCTCT CTTTCGGGAT GAAGTTTTCC ACATTCACAA AGCTGCAGAA GACTTATTTG 
     1981 TAAACATTCG GGGCTACAAT AAACGTATTA ACGACATAAG AGAATGCAAG GAGGCGGCTG 
     2041 TATCCCACGC CGGCTCAATG CACAGAGAAA GACGCAAGTT TTTACGGTCT GCACTAAAAG 
     2101 AACTGGCCAC TGTCCTCTCT GACCAGCCTG GCCTGCTTGG TCCCAAGGCA CTTTTTGTTT 
     2161 TCATGGCGTT ATCTTTTGCC CGTGATGAAA TTATCTGGCT GCTCCGTCAC GCGGACAACA 
     2221 TGCCAAAGAA GAGTGCAGAT GACTTTATAG ATAAGCACAT TGCTGAGCTA ATATTTTACA 
     2281 TGGAAGAGCT TAGAGCACAT GTCAGGAAGT ATGGGCCTGT CATGCAGAGG TACTACGTGC 
     2341 AATACCTCTC TGGCTTTGAT GCTGTTGTCC TCAATGAACT TGTGCAGAAC CTGTCTGTTT 
     2401 GCCCTGAGGA TGAATCGATC ATCATGTCCT CTTTTGTGAA CACCATGACC TCCCTAAGTG 
     2461 TGAAGCAAGT TGAAGATGGA GAAGTGTTTG ATTTCAGAGG GATGAGATTA GATTGGTTTA 
     2521 GGTTACAGGC GTACACTAGC GTCTCCAAAG CTTCACTTAG CCTTGCAGAT CACAGAGAAC 
     2581 TTGGAAAGAT GATGAACACA ATCATATTCC ATACAAAAAT GGTAGATTCC TTGGTTGAAA 
     2641 TGTTGGTGGA AACATCCGAT CTCTCCATAT TTTGTTTTTA CAGCCGTGCT TTTGAGAAGA 
     2701 TGTTTCAGCA GTGCTTGGAG CTGCCCTCTC AGTCAAGATA CTCCATTGCC TTTCCTCTGC 
     2761 TCTGCACTCA CTTCATGAGC TGCACACATG AACTGTGTCC TGAAGAGCGC CATCATATAG 
     2821 GAGATCGTAG CCTTTCCCTG TGCAATATGT TCCTGGATGA GATGGCCAAA CAAGCTCGAA 
     2881 ATCTCATCAC CGATATTTGC ACAGAACAGT GTACTCTTAG TGACCAGTTA TTGCCGAAGC 
     2941 ATTGTGCCAA AACCATCAGT CAAGCAGTGA ATAAGAAGTC AAAAAAACAG ACGGGCAAGA 
     3001 AAGGGGAACC TGAAAGGGAA AAACCGGGTG TGGAGAGCAT GAGGAAAAAC AGGCTGGTAG 
     3061 TGACCAACCT TGATAAGTTG CACACTGCAC TTTCGGAGTT ATGCTTCTCC ATAAATTATG 
     3121 TTCCAAACAT GGCGGTGTGG GAGCACACCT TCACGCCGAG GGAGTATTTG ACTTCTCATC 
     3181 TGGAAATCCG GTTCACTAAA TCAATTGTTG GAATGACTAT GTATAATCAA GCTACACAGG 
     3241 AAATTGCAAA GCCATCAGAG CTTCTAACAA GCGTAAGAGC ATATATGACT GTACTCCAGT 
     3301 CCATAGAGAA CTATGTGCAG ATTGATATCA CCAGAGTATT TAATAATGTC CTTCTTCAGC 
     3361 AAACACAACA CTTAGACAGC CATGGAGAAC CAACCATCAC GAGTCTGTAT ACAAACTGGT 
     3421 ACTTGGAAAC TTTATTAAGA CAAGTCAGCA ATGGCCATAT AGCTTATTTT CCTGCAATGA 
     3481 AAGCATTTGT GAATTTACCC ACAGAAAATG AATTAACATT CAATGCAGAG GAATATTCTG 
     3541 ATATATCAGA AATGAGATCA TTATCAGAGC TCCTAGGCCC ATATGGGATG AAGTTCCTGA 
     3601 GTGAGAGCCT TATGTGGCAC ATTTCATCAC AAGTAGCTGA ACTTAAGAAA CTTGTGGTGG 
     3661 AGAATGTTGA TGTTCTAACA CAAATGAGGA CCAGCTTTGA CAAACCAGAC CAGATGGCTG 
     3721 CACTCTTTAA AAGATTATCA TCTGTTGACA GTGTCTTGAA AAGGATGACG ATAATTGGTG 
     3781 TTATTTTATC CTTCCGGTCA TTGGCACAAG AAGCATTGAG AGATGTCCTG TCCTACCACA 
     3841 TTCCTTTCCT CGTGAGCTCC ATTGAAGATT TCAAGGATCA CATTCCTCGG GAAACTGATA 
     3901 TGAAGGTTGC AATGAATGTG TATGAGCTGT CCTCGGCTGC TGGGTTACCA TGTGAGATTG 
     3961 ATCCTGCCTT AGTCGTAGCT CTTTCCTCAC AAAAATCAGA GAACATTAGT CCAGAGGAAG 
     4021 AATACAAGAT CGCTTGTCTC CTGATGGTCT TTGTAGCAGT TTCCTTGCCT ACACTGGCCA 
     4081 GCAATGTAAT GTCACAGTAC AGCCCTGCAA TAGAAGGACA CTGCAACAAT ATACATTGTT 
     4141 TGGCCAAAGC CATCAACCAG ATTGCTGCAG CTTTGTTTAC AATTCACAAA GGAAGTATTG 
     4201 AAGACCGACT TAAGGAATTC CTGGCGCTTG CATCATCCAG TCTACTGAAA ATTGGCCAAG 
     4261 AGACAGATAA AACTACAACA AGAAATAGAG AATCTGTTTA TTTGCTACTG GATATGATTG 
     4321 TACAAGAATC ACCATTCCTG ACAATGGATC TCTTGGAATC TTGTTTTCCT TATGTGTTGC 
     4381 TGAGAAATGC ATACCATGCT GTCTACAAAC AAAGTGTTAC ATCTTCTGCA ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//