LOCUS       MR231611                8277 bp    DNA     circular  SYN 
DEFINITION  Jak2 (myc-DDK-tagged) - Mouse Janus kinase 2 (Jak2), transcript variant 1
ACCESSION   NM_008413
VERSION     NM_008413.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="Jak2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGGCC TGCCTTACAA TGACAGAAAT GGAGGCAACC TCCACATCTC 
     1081 CTGTACATCA GAATGGTGAT ATTCCTGGAA GTGCTAATTC TGTGAAGCAG ATAGAGCCAG 
     1141 TCCTTCAAGT GTATCTGTAC CATTCTCTTG GGCAAGCTGA AGGAGAGTAT CTGAAGTTTC 
     1201 CAAGTGGAGA GTATGTTGCA GAAGAAATTT GTGTGGCTGC TTCTAAAGCT TGTGGTATTA 
     1261 CGCCTGTGTA TCATAATATG TTTGCGTTAA TGAGTGAAAC CGAAAGGATC TGGTACCCAC 
     1321 CCAATCATGT CTTCCACATA GACGAGTCAA CCAGGCATGA CATACTCTAC AGGATAAGGT 
     1381 TCTACTTCCC TCATTGGTAC TGTAGTGGCA GCAGCAGAAC CTACAGATAC GGAGTGTCCC 
     1441 GTGGGGCTGA AGCTCCTCTG CTTGATGACT TTGTCATGTC TTACCTTTTT GCTCAGTGGC 
     1501 GGCATGATTT TGTTCACGGA TGGATAAAAG TACCTGTGAC TCATGAAACT CAGGAAGAGT 
     1561 GTCTTGGGAT GGCGGTGTTA GACATGATGA GAATAGCTAA GGAGAAAGAC CAGACTCCAC 
     1621 TGGCTGTCTA TAACTCTGTC AGCTACAAGA CATTCTTACC AAAGTGCGTT CGAGCGAAGA 
     1681 TCCAAGACTA TCACATTTTA ACCCGGAAGC GAATCAGGTA CAGATTTCGC AGATTCATTC 
     1741 AGCAATTCAG TCAATGTAAA GCCACTGCCA GGAACCTAAA ACTTAAGTAT CTTATAAACC 
     1801 TGGAAACCCT GCAGTCTGCC TTCTACACAG AACAGTTTGA AGTAAAAGAA TCTGCAAGAG 
     1861 GTCCTTCAGG TGAGGAGATT TTTGCAACCA TTATAATAAC TGGAAACGGT GGAATTCAGT 
     1921 GGTCAAGAGG GAAACATAAG GAAAGTGAGA CACTGACAGA ACAGGACGTA CAGTTATATT 
     1981 GTGATTTCCC TGATATTATT GATGTCAGTA TTAAGCAAGC AAACCAGGAA TGCTCAAATG 
     2041 AAAGTAGAAT TGTAACTGTC CATAAACAAG ATGGTAAAGT TTTGGAGATA GAACTTAGCT 
     2101 CATTAAAAGA AGCCTTGTCA TTCGTGTCAT TAATTGACGG GTATTACAGA CTAACTGCGG 
     2161 ATGCGCACCA TTACCTCTGC AAAGAGGTGG CTCCCCCAGC TGTGCTCGAG AACATACACA 
     2221 GCAACTGCCA CGGCCCAATA TCAATGGATT TTGCCATTAG CAAACTAAAG AAGGCGGGTA 
     2281 ACCAGACTGG ACTATATGTG CTACGATGCA GCCCTAAGGA CTTCAACAAA TACTTTCTGA 
     2341 CCTTTGCTGT TGAGCGAGAA AATGTCATTG AATATAAACA CTGTTTGATT ACGAAGAATG 
     2401 AGAATGGAGA ATACAACCTC AGCGGGACTA AGAGGAACTT CAGTAACCTT AAGGACCTTT 
     2461 TGAATTGCTA CCAGATGGAA ACTGTGCGCT CAGACAGTAT CATCTTCCAG TTTACCAAAT 
     2521 GCTGCCCCCC AAAGCCAAAA GATAAATCAA ACCTTCTCGT CTTCAGAACA AATGGTATTT 
     2581 CTGATGTTCA GATCTCACCA ACATTACAGA GGCATAATAA TGTGAATCAA ATGGTGTTTC 
     2641 ACAAAATCAG GAATGAAGAT TTAATATTTA ATGAAAGTCT TGGCCAAGGT ACTTTTACAA 
     2701 AAATTTTTAA AGGTGTAAGA AGAGAAGTTG GAGATTATGG TCAACTGCAC AAAACGGAAG 
     2761 TTCTTTTGAA AGTCCTAGAT AAAGCACATA GGAACTATTC AGAGTCTTTC TTCGAAGCAG 
     2821 CAAGCATGAT GAGTCAGCTT TCTCACAAGC ATTTGGTTTT GAATTATGGT GTCTGTGTCT 
     2881 GTGGAGAGGA GAACATTCTG GTTCAAGAAT TTGTAAAATT TGGATCACTG GATACATACC 
     2941 TGAAGAAGAA CAAAAATTCC ATAAATATAT TATGGAAACT TGGAGTGGCT AAGCAGTTGG 
     3001 CATGGGCCAT GCATTTTCTA GAAGAAAAAT CCCTTATTCA TGGGAATGTG TGTGCTAAAA 
     3061 ATATCCTGCT TATCAGAGAA GAAGACAGGA GAACGGGGAA CCCACCTTTC ATCAAACTTA 
     3121 GTGATCCTGG CATTAGCATT ACAGTTCTAC CGAAGGACAT TCTTCAGGAG AGAATACCAT 
     3181 GGGTACCTCC TGAATGCATT GAGAATCCTA AAAATCTCAA TCTGGCAACA GACAAGTGGA 
     3241 GCTTCGGGAC CACTCTGTGG GAGATCTGCA GTGGAGGAGA TAAGCCCCTG AGTGCTCTGG 
     3301 ATTCTCAAAG AAAGCTGCAG TTCTATGAAG ATAAGCATCA GCTTCCTGCA CCCAAGTGGA 
     3361 CAGAGTTAGC AAACCTTATA AATAATTGCA TGGACTATGA GCCAGATTTC AGGCCTGCTT 
     3421 TCAGAGCTGT CATCCGTGAT CTTAACAGCC TGTTTACTCC AGATTATGAA CTACTAACAG 
     3481 AAAATGACAT GCTACCAAAC ATGAGAATAG GTGCCCTAGG GTTTTCTGGT GCTTTTGAAG 
     3541 ACAGGGACCC TACACAGTTT GAAGAGAGAC ACTTGAAGTT TCTACAGCAG CTTGGCAAAG 
     3601 GTAACTTCGG GAGTGTGGAG ATGTGCCGCT ATGACCCGCT GCAGGACAAC ACTGGCGAGG 
     3661 TGGTCGCTGT GAAGAAACTC CAGCACAGCA CTGAAGAGCA CCTCCGAGAC TTTGAGAGGG 
     3721 AGATCGAGAT CCTGAAATCC TTGCAGCATG ACAACATCGT CAAGTACAAG GGAGTGTGCT 
     3781 ACAGTGCGGG TCGGCGCAAC CTAAGATTAA TTATGGAATA TTTACCATAT GGAAGTTTAC 
     3841 GAGACTATCT CCAAAAACAT AAAGAACGGA TAGATCACAA AAAACTTCTT CAATACACAT 
     3901 CTCAGATATG CAAGGGCATG GAATATCTTG GTACAAAAAG GTATATCCAC AGGGACCTGG 
     3961 CAACAAGGAA CATATTGGTG GAAAATGAGA ACAGGGTTAA AATAGGAGAC TTCGGATTAA 
     4021 CCAAAGTCTT GCCGCAGGAC AAAGAATACT ACAAAGTAAA GGAGCCAGGG GAAAGCCCCA 
     4081 TATTCTGGTA CGCACCTGAA TCCTTGACGG AGAGCAAGTT TTCTGTGGCC TCAGATGTGT 
     4141 GGAGCTTTGG AGTGGTTCTA TACGAACTTT TCACATACAT CGAGAAGAGT AAAAGTCCAC 
     4201 CCGTGGAATT TATGCGAATG ATTGGCAATG ATAAACAAGG GCAAATGATT GTGTTCCATT 
     4261 TGATAGAGCT ACTGAAGAGC AACGGAAGAT TGCCAAGGCC AGAAGGATGC CCAGATGAGA 
     4321 TTTATGTGAT CATGACAGAG TGCTGGAACA ACAATGTGAG CCAGCGTCCC TCCTTCAGGG 
     4381 ACCTTTCCCT TCGGGTGGAT CAAATCCGGG ACAGTATAGC TGCGACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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