LOCUS       MR231602                8253 bp    DNA     circular  SYN 
DEFINITION  Asap1 (myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 3
ACCESSION   NM_001276462
VERSION     NM_001276462.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4410..4501 
                     /label="Myc-DDK"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    4588..4607 
                     /label="Restriction Enzyme"  
     misc_feature    5392..6064 
                     /label="ColE1"  
     misc_feature    6494..7288 
                     /label="Kan/Neo"  
     misc_feature    7323..7625 
                     /label="SV40 ori"  
     misc_feature    7711..8188 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4392..4398 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4392 
                     /label="Asap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGACCAG ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT 
     1081 ACAACTCGCC CACCACGTCC AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA 
     1141 CGCTGCTGGA GGAGGCTCTA GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG 
     1201 TAAAAGCAAT ATATAATTCC GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG 
     1261 TTCTGGATAA GTTTGGAAGT AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT 
     1321 TTGTCAAGTT TTCTACACTC ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG 
     1381 GTCTGAGCCA CAATGTGATC TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG 
     1441 TCAAAGGAGA TCTCAAGAAG CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA 
     1501 CAAAAATTGA GAAGGAGAAA AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA 
     1561 TAACAGGCGC TGAGATTGCA GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA 
     1621 TGTGTGAATA TCTCATTAAA GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC 
     1681 AGAACCTTAT AAAGTATTAC CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG 
     1741 CTGATAAACT GAAACAGTAC ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA 
     1801 CCCAAGATGA AGAAAAAAAA CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC 
     1861 AACTTGATCC GAAAGAAGTA GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTGACTCTC 
     1921 AGAGCCGGCA AGGTGGGTAC AGCATGCATC AGCTCCAGGG CAACAAGGAG TACGGCAGTG 
     1981 AAAAGAAGGG CTTCCTGCTG AAGAAGAGTG ATGGGATCCG GAAAGTGTGG CAGAGACGGA 
     2041 AGTGTGCGGT CAAGAACGGG ATCCTGACCA TCTCCCACGC AACTTCCAAC AGGCAGCCTG 
     2101 CTAAGTTAAA CCTCCTCACC TGCCAAGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG 
     2161 ACCTGATATC ACATAATCGA ACATATCACT TTCAAGCAGA AGATGAGCAG GATTATATAG 
     2221 CGTGGATATC AGTACTGACG AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG 
     2281 AGCAGAGCAC AGGGGAGAAT AGCCTGGAGG ACCTTACCAA AGCCATCATC GAGGATGTAC 
     2341 AGCGGCTCCC TGGGAATGAC ATCTGCTGTG ACTGCGGCTC ATCAGAACCC ACGTGGCTTT 
     2401 CAACCAACTT GGGTATTTTG ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG 
     2461 TTCATATTTC TCGCATTCAG TCTTTGGAAC TAGACAAATT AGGAACTTCC GAACTCTTGC 
     2521 TGGCCAAGAA TGTAGGAAAC AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC 
     2581 CTTCACCAAA GCCCACCCCT TCAAGTGATA TGACCGTGCG TAAAGAGTAC ATCACTGCAA 
     2641 AGTATGTCGA CCACCGCTTC TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAACG 
     2701 AATTGCTTGA GGCCATCAAG TCCAGGGACT TACTTGCATT AATCCAAGTC TATGCAGAGG 
     2761 GGGTGGAGTT AATGGAGCCA CTGCTGGAAC CTGGGCAGGA GCTCGGGGAG ACAGCCCTTC 
     2821 ATCTTGCCGT CAGGACCGCA GACCAGACAT CTCTCCATCT GGTTGATTTC CTTGTACAAA 
     2881 ACTGTGGGAA CCTGGATAAG CAAACGTCTG TGGGGAATAC AGTTCTGCAC TATTGCAGCA 
     2941 TGTACGGCAA GCCGGAGTGT CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATCG 
     3001 TTAACCAGAA TGGAGAAACT GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACCCAGTGTG 
     3061 AAGATCTGCT TTCCCAGGCT AAATCTGGAA AGTTCAACCC TCATGTCCAC GTAGAGTATG 
     3121 AGTGGAATCT TCGACAGGAC GAGATGGATG AGAGCGATGA CGATCTGGAT GACAAGCCAA 
     3181 GCCCGATCAA AAAGGAACGC TCGCCCAGAC CACAGAGCTT CTGCCACTCC TCCAGCATCT 
     3241 CGCCACAGGA CAAGCTGGCA CTGCCAGGGT TCAGCACTCC ACGGGACAAG CAGCGGCTCT 
     3301 CCTACGGAGC CTTCACCAAC CAGATCTTCG CCTCTACGAG CACAGATTTG CCCACATCAC 
     3361 CCACCAGTGA GGCTCCCCCT TTGCCACCTC GGAACGCCGG GAAAGGTCCA ACTGGCCCAC 
     3421 CTTCAACACT CCCTCTAGGC ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA 
     3481 GGCCTCCTCC CCCACCACCA GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC 
     3541 CTCACGGGCC CCCAAACAAA GGCGCAATTC CTTGGGGTAA TGATGTGGGC CCATTATCTT 
     3601 CAAGTAAGAC GGCCAACAAG TTTGAGGGGC TGTCTCAGCA AGCAAGCACC AGTTCTGCTA 
     3661 AGACTGCCCT TGGCCCGAGA GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA 
     3721 CGGAGACCAG CCATCATCTC TCCCTCGACA GAACCAACAT CCCACCTGAG ACTTTTCAGA 
     3781 AATCATCACA GTTGACAGAG TTACCCCAAA AGCCACCACT TGGAGAGCTG CCCCCGAAGC 
     3841 CTGTGGAACT GGCCCCCAAG CCCCAAGTTG GAGAGCTGCC ACCTAAGCCT GGAGAGCTAC 
     3901 CCCCTAAGCC CCAATTAGGT GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA 
     3961 AGCCACAGAT GAAGGACCTG CCCCCTAAGC CGCAGCTGGG GGATCTGCTG GCAAAGTCCC 
     4021 AGGCTGGCGA TGTCTCAGCC AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA 
     4081 CCGGGGATCT GTCTCCAAAT GTACAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAAG 
     4141 ACTCCAACGA CCTCACACCC ACGCTGCCAG AGACACCCGT ACCACTGCCG AGAAAAATCA 
     4201 ATACGGGGAA AAATAAAGTG AGGCGGGTGA AGACCATTTA TGACTGCCAG GCAGATAATG 
     4261 ACGACGAACT CACATTTATT GAGGGGGAAG TGATCATTGT CACCGGGGAA GAGGACCAGG 
     4321 AGTGGTGGAT CGGGCATATC GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT 
     4381 TTGTCCACAT CCTGTCTGAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4441 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6841 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6901 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6961 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7021 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7081 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7141 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7201 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7261 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7321 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7381 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7441 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7501 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7681 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7741 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7801 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7861 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7921 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7981 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8041 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8101 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8161 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8221 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//