LOCUS       MR231601                8247 bp    DNA     circular  SYN 
DEFINITION  Bag6 (myc-DDK-tagged) - Mouse BCL2-associated athanogene 6 (Bag6), transcript variant 3
ACCESSION   NM_001252469
VERSION     NM_001252469.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="Bag6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGAGT GATAGTGCCA GTACCGCTAT GGAGGAGCCT GACAGCCTGG 
     1081 AGGTACTGGT GAAGACCCTG GACTCTCAGA CTCGGACTTT TATTGTGGGG GCCCAGATGA 
     1141 ATGTAAAGGA GTTTAAGGAA CACATAGCTG CCTCTGTCAG CATCCCTTCC GAGAAACAGC 
     1201 GGCTCATCTA CCAGGGCCGG GTCCTACAAG ACGACAAGAA GCTCCAGGAG TACAACGTTG 
     1261 GGGGAAAGGT TATTCACCTG GTGGAACGGG CTCCTCCTCA GACTCAGCTT CCTTCTGGAG 
     1321 CATCTTCTGG GACAGGCTCT GCCTCAGCAA CTCATGGTGG GGCACCCCTG CCTGGCACTC 
     1381 GGGGCCCTGG GGCTTCTGTT CATGACCGGA ATGCCAACAG CTATGTCATG GTTGGAACCT 
     1441 TCAATCTTCC TAGTGAGCCC CGGGTCCGGC TGGTGATGGC TCAGCACATG ATTAGGGATA 
     1501 TACAGACCTT ACTGTCCCGG ATGGAGTGTC GAGGGGGAAC CCAAGCTCAG GCCAGTCAGC 
     1561 CACCCCCGCA GACACCGCAG ACTGTGGCCT CAGAGACGGT AGCCTTGAAC TCACAAACAT 
     1621 CAGAACCAGT CGAAAGTGAA GCTCCTCCTC GAGAGCCCAT GGAGTCAGAA GAAATGGAGG 
     1681 AACGTCCCCC AACCCAGACT CCAGAGCTTG CGCCCTCAGG CCCAGCTCCC GCAGGCCCAG 
     1741 CTCCCGCAGG CCCAGCTCCC GCGCCAGAGA CAAATGCACC CAACCACCCT TCCCCCGCAG 
     1801 AGCACGTGGA GGTGCTCCAG GAGCTTCAGC GCTTGCAGCG CCGTCTTCAG CCTTTCCTAC 
     1861 AGCGCTACTG CGAGGTCCTG GGCGCTGCTG CCACCACAGA CTACAACAAC AACCATGAGG 
     1921 GCCGTGAGGA GGACCAGAGG TTGATCAACT TGGTTGGGGA GAGCCTGCGG CTACTGGGCA 
     1981 ACACTTTCGT GGCATTGTCT GATCTGCGCT GCAATCTAGC CTGTGCACCC CCACGGCACC 
     2041 TGCACGTGGT TCGGCCTATG TCTCACTACA CGACTCCCAT GGTGCTCCAG CAGGCAGCCA 
     2101 TTCCCATTCA GATCAATGTG GGGACTACTG TGACCATGAC AGGCAATGGG GCTCGGCCTC 
     2161 CACCAGCTCC TGGTGCGGAG GCAGCAACCC CAGGTTCTGC CCAGGCCACA TCCCTGCCTC 
     2221 CCTCTTCCAC CACTGTTGAT TCATCAACTG AAGGAGCTCC CCCACCAGGG CCAGCACCGC 
     2281 CACCAGCCTC CAGCCACCCA CGGGTCATCC GGATTTCCCA CCAGAGTGTG GAGCCCGTGG 
     2341 TCATGATGCA CATGAACATT CAAGATTCTG GAGCACAGCC TGGTGGTGTG CCAAGTGCTC 
     2401 CCACTGGTCC ACTGGGACCT CCTGGTCATG GACAGACCCT GGGCTCCACC CTCATCCAGC 
     2461 TGCCCTCCCT GCCCCCTGAG TTCATGCACG CCGTCGCCCA CCAGATCACT CATCAGGCCA 
     2521 TGGTGGCAGC TGTTGCCTCC GCGGCCGCAG GACAGCAGGT GCCCGGCTTC CCAACAGCAC 
     2581 CAACTCGGGT GGTGATTGCC CGGCCAACTC CTCCACAGGC TCGGCCTTCC CATCCTGGGG 
     2641 GACCTCCAGT CTCTGGAGCT CTGGGCGCTG GGCTGGGTAC AAACACTTCA TTGGCCCAGA 
     2701 TGGTGAGCGG CCTTGTGGGG CAACTTCTTA TGCAGCCTGT TCTTGTGGCT CAGGGGACTC 
     2761 CAGGAATGGC TCAGGCTCAG GCCCAGGCCC AGGCTCAGGC CCAGGCCCAG GCCCAGGCTC 
     2821 CAGCTCCGGC TCCGGCTCCA GCTCCAGCTC CTGCCACTGC TTCAGCTAGT GCTGGTACTA 
     2881 CCAACACAGC TACCACAGCT GGCCCTGCTC CTGGGGGTCC TGCCCAGCCT CCACCTCCTC 
     2941 AGCCCTCTGC AGCTGATCTT CAGTTCTCTC AGCTCCTGGG AAATCTGCTG GGGCCTGCAG 
     3001 GGCCAGGGGC TGGGGGGCCA GGCATGGCTT CTCCCACCAT CACTGTGGCA ATGCCTGGTG 
     3061 TCCCTGCTTT TCTCCAGGGC ATGACTGATT TTTTGCAGGC ATCACAGACT GCCCCTCCAC 
     3121 CCCCTCCACC TCCTCCACCC CCACCCCCTG CCCCAGAGCA GCAGAGCACA CCCCCACCAG 
     3181 GGTCTCCTTC TGGTGGAACA GCAAGCCCTG GAGGCTTAGG TCCTGAGAGC CTGCCACCAG 
     3241 AGTTTTTCAC CTCAGTGGTG CAGGGCGTGT TGAGCTCCCT CCTGGGCTCC TTGGGGGCTC 
     3301 GAGCTGGCAG CAGTGAGAGT ATCGCTGCCT TCATCCAACG CCTCAGTGGA TCCAGCAACA 
     3361 TCTTTGAGCC TGGGGCTGAT GGCGCCCTTG GATTCTTCGG AGCTCTGCTC TCTCTCCTGT 
     3421 GCCAGAATTT CTCCATGGTG GATGTGGTGA TGCTTCTCCA TGGCCATTTC CAGCCACTGC 
     3481 AGCGGCTCCA GCCACAGCTG CGATCTTTCT TCCACCAGCA CTACCTGGGT GGCCAGGAGC 
     3541 CCACGCCTAG CAACATCCGG ATGGCGACCC ACACACTGAT CACTGGGCTG GAAGAGTATG 
     3601 TAAGGGAGAG TTTTTCTTTG GTACAGGTTC AGCCAGGTGT GGATATCATC CGGACAAATT 
     3661 TAGAATTTCT CCAAGAGCAG TTTAACAGCA TTGCTGCTCA TGTGCTGCAC TGTACAGACA 
     3721 GTGGATTTGG AGCCCGGTTG CTGGAGCTGT GTAACCAGGG CCTGTTTGAA TGCTTGGCCC 
     3781 TGAACCTCCA CTGCTTGGGG GGACAGCAAA TGGAGCTTGC TGCTGTCATC AATGGTCGAA 
     3841 TTCGCCGCAT GTCTCGCGGG GTGAATCCAT CCTTGGTGAG CTGGCTGACA ACCATGATGG 
     3901 GACTGAGGCT TCAGGTGGTC TTGGAGCACA TGCCTGTGGG TCCCGACGCC ATCCTCAGAT 
     3961 ATGTTCGTAG GGTTGGTGAT CCTCCTCAGA CACTTCCTGA AGAGCCGATG GAAGTTCAGG 
     4021 GAGCAGAAAG AACTTCCCCT GAACCTCAGA GAGAGAATGC TTCCCCAGCC CCTGGAACAA 
     4081 CAGCAGAAGA AGCCATGTCC CGAGGCCCGC CCCCTGCTCC TGAAGGAGGT TCCCGAGATG 
     4141 AACAGGATGG AGCTTCAGCT GATGCAGAAC CCTGGGCAGC TGCAGTCCCC CCTGAATGGG 
     4201 TCCCTATTAT CCAGCAGGAC ATTCAGAGCC AGCGGAAGGT GAAACCTCAG CCGCCCCTGA 
     4261 GTGATGCCTA CCTCAGTGGC ATGCCTGCCA AGAGACGAAA GCTCCGGTCT GATATCCAAA 
     4321 AACGACTGCA GGAAGATCCC AACTACAGCC CCCAGCGCTT CCCTAATGCC CATCGGGCAT 
     4381 TTGCTGATGA CCCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//