LOCUS       MR231600                8247 bp    DNA     circular  SYN 
DEFINITION  Tek (myc-DDK-tagged) - Mouse endothelial-specific receptor tyrosine kinase (Tek), transcript variant 2
ACCESSION   NM_001290549
VERSION     NM_001290549.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="Tek"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCTTTA GCCGGCTTAG TTCTCTGTGG AGTCAGCTTG CTCCTTTATG 
     1081 GAGTAGTAGA AGGCGCCATG GACCTGATCT TGATCAATTC CCTACCTCTT GTGTCTGATG 
     1141 CCGAAACATC CCTCACCTGC ATTGCCTCTG GGTGGCACCC CCATGAGCCC ATCACCATAG 
     1201 GAAGGGACTT TGAAGCCTTA ATGAACCAGC ACCAAGATCC ACTGGAGGTT ACTCAAGATG 
     1261 TGACCAGAGA ATGGGCGAAA AAAGTTGTTT GGAAGAGAGA AAAGGCCAGT AAGATTAATG 
     1321 GTGCTTATTT CTGTGAAGGT CGAGTTCGAG GACAGGCTAT AAGGATACGG ACCATGAAGA 
     1381 TGCGTCAACA AGCATCCTTC CTACCTGCTA CTTTAACTAT GACCGTGGAC AGGGGAGATA 
     1441 ATGTGAACAT ATCTTTCAAA AAGGTGTTAA TTAAAGAAGA AGATGCAGTG ATTTACAAAA 
     1501 ATGGCTCCTT CATCCACTCA GTGCCCCGGC ATGAAGTACC TGATATTTTA GAAGTTCACT 
     1561 TGCCGCATGC TCAGCCCCAG GATGCTGGTG TGTACTCGGC CAGGTACATA GGAGGAAACC 
     1621 TGTTCACCTC AGCCTTCACC AGGCTGATTG TTCGGAGATG TGAAGCTCAG AAGTGGGGGC 
     1681 CCGACTGTAG CCGTCCTTGT ACTACTTGCA AGAACAATGG AGTCTGCCAT GAAGATACCG 
     1741 GGGAATGCAT TTGCCCTCCT GGGTTTATGG GGAGAACATG TGAGAAAGCT TGTGAGCCGC 
     1801 ACACATTTGG CAGGACCTGT AAAGAAAGGT GTAGTGGACC AGAAGGATGC AAGTCTTATG 
     1861 TGTTCTGTCT CCCAGACCCT TACGGGTGTT CCTGTGCCAC AGGCTGGAGG GGGTTGCAGT 
     1921 GCAATGAAGC ATGCCCATCT GGTTACTACG GACCAGACTG TAAGCTCAGG TGCCACTGTA 
     1981 CCAATGAAGA GATATGTGAT CGGTTCCAAG GATGCCTCTG CTCTCAAGGA TGGCAAGGGC 
     2041 TGCAGTGTGA GAAAGAAGGC AGGCCAAGGA TGACTCCACA GATAGAGGAT TTGCCAGATC 
     2101 ACATTGAAGT AAACAGTGGA AAATTTAACC CCATCTGCAA AGCCTCTGGG TGGCCACTAC 
     2161 CTACTAGTGA AGAAATGACC CTAGTGAAGC CAGATGGGAC AGTGCTCCAA CCAAATGACT 
     2221 TCAACTATAC AGATCGTTTC TCAGTGGCCA TATTCACTGT CAACCGAGTC TTACCTCCTG 
     2281 ACTCAGGAGT CTGGGTCTGC AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCTTTCA 
     2341 ACATTTCCGT CAAAGTTCTT CCAGAGCCCC TGCACGCCCC AAATGTGATT GACACTGGAC 
     2401 ATAACTTTGC TATCATCAAT ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCCATCAAAT 
     2461 CCAAGAAGCT TTTCTATAAA CCTGTCAATC AGGCCTGGAA ATACATTGAA GTGACGAATG 
     2521 AGATTTTCAC TCTCAACTAC TTGGAGCCGC GGACTGACTA CGAGCTGTGT GTGCAGCTGG 
     2581 CCCGTCCTGG AGAGGGTGGA GAAGGGCATC CTGGGCCTGT GAGACGATTT ACAACAGCGT 
     2641 CTATCGGACT CCCTCCTCCA AGAGGTCTCA GTCTCCTGCC AAAAAGCCAG ACAGCTCTAA 
     2701 ATTTGACTTG GCAACCGATA TTTACAAACT CAGAAGATGA ATTTTATGTG GAAGTCGAGA 
     2761 GGCGATCCCT GCAAACAACA AGTGATCAGC AGAACATCAA AGTGCCTGGG AACCTGACCT 
     2821 CGGTGCTACT GAGCAACTTA GTCCCCAGGG AGCAGTACAC AGTCCGAGCT AGAGTCAACA 
     2881 CCAAGGCGCA GGGGGAGTGG AGTGAAGAAC TCAGGGCCTG GACCCTTAGT GACATTCTCC 
     2941 CTCCTCAACC AGAAAACATC AAGATCTCCA ACATCACTGA CTCCACAGCT ATGGTTTCTT 
     3001 GGACAATAGT GGATGGCTAT TCGATTTCTT CCATCATCAT CCGGTATAAG GTTCAGGGCA 
     3061 AAAATGAAGA CCAGCACATT GATGTGAAGA TCAAGAATGC TACCGTTACT CAGTACCAGC 
     3121 TCAAGGGCCT AGAGCCAGAG ACTACATACC ATGTGGATAT TTTTGCTGAG AACAACATAG 
     3181 GATCAAGCAA CCCAGCCTTT TCTCATGAAC TGAGGACGCT TCCACATTCC CCAGCCTCTG 
     3241 CAGACCTCGG AGGGGGAAAG ATGCTACTCA TAGCCATCCT TGGGTCGGCT GGAATGACTT 
     3301 GCATCACCGT GCTGTTGGCG TTTCTGATTA TGTTGCAACT GAAGAGAGCA AATGTCCAAA 
     3361 GGAGAATGGC TCAGGCATTC CAGAACAGAG AAGAACCAGC TGTGCAGTTT AACTCAGGAA 
     3421 CTCTGGCCCT TAACAGGAAG GCCAAAAACA ATCCGGATCC CACAATTTAT CCTGTGCTTG 
     3481 ACTGGAATGA CATCAAGTTT CAAGACGTGA TCGGAGAGGG CAACTTTGGC CAGGTTCTGA 
     3541 AGGCACGCAT CAAGAAGGAT GGGTTACGGA TGGATGCCGC CATCAAGAGG ATGAAAGAGT 
     3601 ATGCCTCCAA AGATGATCAC AGGGACTTCG CAGGAGAACT GGAGGTTCTT TGTAAACTTG 
     3661 GACACCATCC AAACATCATT AATCTCTTGG GAGCATGTGA ACACCGAGGC TATTTGTACC 
     3721 TAGCTATTGA GTATGCCCCG CATGGAAACC TCCTGGACTT CCTGCGTAAG AGCAGAGTGC 
     3781 TAGAGACAGA CCCTGCTTTT GCCATCGCCA ACAGTACAGC TTCCACACTG TCCTCCCAAC 
     3841 AGCTTCTTCA TTTTGCTGCA GATGTGGCCC GGGGGATGGA CTACTTGAGC CAGAAACAGT 
     3901 TTATCCACAG GGACCTGGCT GCCAGAAACA TTTTAGTTGG TGAAAACTAC ATAGCCAAAA 
     3961 TAGCAGATTT TGGATTGTCA CGAGGTCAAG AAGTGTATGT GAAAAAGACA ATGGGAAGGC 
     4021 TCCCAGTGCG TTGGATGGCA ATCGAATCAC TGAACTATAG TGTCTATACA ACCAACAGTG 
     4081 ATGTCTGGTC CTATGGTGTA TTGCTCTGGG AGATTGTTAG CTTAGGAGGC ACCCCCTACT 
     4141 GCGGCATGAC GTGCGCGGAG CTCTATGAGA AGCTACCCCA GGGCTACAGG CTGGAGAAGC 
     4201 CCCTGAACTG TGATGATGAG GTGTATGATC TAATGAGACA GTGCTGGAGG GAGAAGCCTT 
     4261 ATGAGAGACC ATCATTTGCC CAGATATTGG TGTCCTTAAA CAGGATGCTG GAAGAACGGA 
     4321 AGACATACGT GAACACCACA CTGTATGAGA AGTTTACCTA TGCAGGAATT GACTGCTCTG 
     4381 CGGAAGAAGC AGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//