LOCUS       MR231596                8244 bp    DNA     circular  SYN 
DEFINITION  Synj2 (myc-DDK-tagged) - Mouse synaptojanin 2 (Synj2), transcript variant 5
ACCESSION   NM_001290698
VERSION     NM_001290698.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4399..4490 
                     /label="Myc-DDK"  
     misc_feature    4520..5105 
                     /label="PolyA signal"  
     misc_feature    4577..4596 
                     /label="Restriction Enzyme"  
     misc_feature    5381..6053 
                     /label="ColE1"  
     misc_feature    6483..7277 
                     /label="Kan/Neo"  
     misc_feature    7312..7614 
                     /label="SV40 ori"  
     misc_feature    7700..8177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4374..4381 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4374 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAGTTG CTCTGGGCTT CTTGCCACGC GGGTGACACA CCTATGATAA 
     1081 ATTTTGACTT CCATCAGTTT GCCAAAGGTA GGAAGCTAGA GAAATTGGAG AACCTGTTGA 
     1141 GACCTCAGTT ACAGCTACAC TGGGAAGACT TCGGCGTGTT TGCGAAGGGC GAGAATGTAA 
     1201 GTCCACGGTT TCAGAAAGGC ACTCTGCGGA TGAACTGTCT CGACTGTCTG GATAGAACCA 
     1261 ACACTGTGCA GTGCTTCATT GCTCTTGAGG TCCTTCATCT GCAGCTTGAG AGCTTGGGGC 
     1321 TAAATTCAAA GCCCATCATT GACCGTTTTG TGGAGTCCTT CAAAGCCATG TGGTCTCTGA 
     1381 ATGGGCACAG CCTGAGCAAG GTGTTCACAG GGAGCAGGGC CCTGGAAGGA AAGGCCAAGG 
     1441 TGGGAAAGCT GAAGGATGGG GCCCGATCCA TGTCTCGCAC CATCCAGTCC AACTTCTTCG 
     1501 ACGGGGTGAA GCAGGAGGCC ATCAAGCTAC TGCTAGTCGG AGATGTCTAC AATGAAGAGT 
     1561 CTACAGACAA AGGACGGATG CTGCTGGACA ACACGGCCCT TCTGGCGACC CCCAGGATCT 
     1621 TGAAGGCCAT GACAGAACGC CAGTCGGAAT TCACGAATTT CAAGCGCATC CAGATTGCTG 
     1681 TGGGGACCTG GAATGTGAAC GGAGGAAAGC AATTCCGTAG CAATCTCCTG GGGACGGCTG 
     1741 AGCTCACGGA CTGGCTCCTA GATGCTCCTC AGCTGTCAGG AGCAGTGGAC TCCCAGGATG 
     1801 ATGGCAGTCC TGCTGACGTA TTTGCCATCG GGTTTGAGGA GATGGTGGAA CTGAGTGCGG 
     1861 GAAATATTGT CAATGCCAGC ACCACCAACA GGAAGATGTG GGGCGAGCAG CTTCAGAAAG 
     1921 CCATCTCCCG CTCCCATCGG TACATCCTCT TGACCTCCGC ACAGCTGGTG GGCGTCTGTC 
     1981 TTTACATCTT TGTACGTCCG TACCACGTCC CGTTCATCAG AGACGTGGCC ATCGACACCG 
     2041 TGAAGACCGG CATGGGGGGA AAGGCGGGGA ATAAGGGTGC TGTGGGCATC CGCTTCCAGC 
     2101 TCCACAGCAC CAGTTTCTGC TTCGTCTGTA GCCACCTGAC GGCTGGGCAG TCTCAGGTGA 
     2161 AGGAGAGGAA TGAAGACTAC CGGGAGATCA CGCACAAACT CTCCTTCCCT TCGGGGAGAA 
     2221 ACATATTTTC ACATGATTAC GTGTTTTGGT GTGGCGATTT CAACTACCGT ATTGATCTTA 
     2281 CTTACGAAGA AGTCTTCTAT TTTGTTAAAC GCCAAGACTG GAAGAAACTT ATGGAATTCG 
     2341 ATCAGTTACA GTTACAGAAA TCAAGTGGAA AAATTTTTAA AGACTTTCAT GAAGGAGCCG 
     2401 TTAACTTCGG ACCCACCTAC AAGTATGACG TTGGATCAGC TGCCTACGAC ACAAGTGACA 
     2461 AGTGCCGTAC CCCAGCCTGG ACAGACAGGG TGCTGTGGTG GAGGAAGAAG CATCCATATG 
     2521 ATAAAACAGC TGGTGAACTC AACCTTCTAG ACAGCGATCT AGACGGCGAT CCCCAAATCA 
     2581 GACACACCTG GTCTCCAGGC ACTCTGAAAT ACTACGGCCG TGCAGAGCTG CAGGCGTCTG 
     2641 ATCACAGACC TGTGCTGGCC ATTGTGGAGG TGGAGGTTCA AGAGGTGGAT GTAGGAGCCC 
     2701 GGGAGAGGGT CTTCCAGGAA GTGTCCTCTG TCCAAGGCCC GCTGGATGCC ACCGTTGTTG 
     2761 TAAACCTCCA GTCTCCAACC CTAGAAGAGA AAAATGAATT TCCAGAGGAC CTGCGCACAG 
     2821 AACTTATGCA GACTTTGGGG AATTATGGGA CGATTATTCT AGTCAGGATC AACCAAGGGC 
     2881 AGATGCTGGT GACGTTTGCA GACAGCCACT CGGCTCTCAG TGTGCTGGAT GTGGATGGTA 
     2941 TGAAGGTGAA AGGCAGGGCC GTGAAGATTC GACCAAAGAC CAAAGATTGG CTAGAAGGCC 
     3001 TGAGAGAGGA GCTTCTCCGG AAGCGAGACA GCATGGCCCC TGTGTCTCCC ACCGCCAACT 
     3061 CCTGCTTGTT GGAGGAGAAC TTTGACTTCT CGAGTCTGGA CTATGAGTCC GAAGGGGATG 
     3121 TTCTTGAAGA GGATGAAGAC TATTTAGTGG ATGGGTTTGG CCAGCCTGTA GTCTCAGACA 
     3181 GTGAGCTCGG TGGAGACAAC TCTTCCGACA CCATGAGCTC CTTGACACCC GCCAGCAAGT 
     3241 CTCCCGCCCT GGCTAAAAAG AAGCAACATC CAACATACAA AGCTGGCTTA ATGGTGAAGA 
     3301 AGTCAGCCTC AGACGCGTCC ATCTCTTCTG GCACTCATGG ACAATATTCC ATCTTGCAGA 
     3361 CAGCGAAACT TCTCCCAGGA GCACCCCAGC AACCACCCAA GGCTAGAACT GGAATAAGTA 
     3421 AACCTTACAA CGTCAAACAG ATCAAAACCA CCAACGCTCA GGAGGCAGAA GCAGCTATCC 
     3481 GGTGTCTCCT GGAAGCTAGC GGAGGGGTCC CGGAATCAGC CCCAGGTGCC ATACCCCTGA 
     3541 GAAACCAAGG GTCTTCTAAG CCAGAGGCCA CCCTGGGGCC CCCAGCCCTG CCCCGCCGGC 
     3601 CTGCTCCAAG GGTTCCCACT ATGAAGAAAC CAACTTTGAG GAGGACAGGA AAGCCCATGT 
     3661 TACCAGAAGA AAACTTTGAG CCACAGCCTG TCCATTTTAC AATGGCTTCC CAGGAAATGA 
     3721 ACCTTGAGAC CCCTCCTCCA ATAACAGCTA CTCCCATCCC GCCTGTTCCC AAACCAAGAA 
     3781 CACTTCAGCC TGGGAAAGGT GTTGAGGGGA GGCCAAGCAG CGGTAAGCCA GAACCAGATG 
     3841 AAGCTCCTTC CGTGACAGGC ACTGTGGAGT CACCACCTCC AGAGGCCCAG GAGGCCCCGT 
     3901 CTCTGGCTCC CAAGGTGCCT CCGAGGAGGA AGAAGTCTGC ACCAGCGGCC TTCCACCTGC 
     3961 AGGTCCTGCA GAACAACAGC CAAGTTCTCC AGGGCCTCAC GTGCTCCAGC AGTTCTCCAC 
     4021 CCTCACTGAA GCCCGACACC CACCCGCTCT GTCTACAAGT GGCACTTGGC ACTTCATCTG 
     4081 CCAGAAGCCC TGAAACCCAT GGCCCCAGAG TGACAGAGCC CGAGGCGGCC TCTTTTCATG 
     4141 GCAACTATCC AGATCCCTTC TGGAGCCTTC TCCACCACCC TAAGCTGTTG AACAATACCT 
     4201 GGCTGTCCAA GAGCTCCGAG CCTTTAGACG TGGGGTCCAG GAACCCTGAG AGGACACACA 
     4261 CAGAGCCGGC ACAGGTCAAT GCATCGTTGG CTGAGAGGGG GCTTCCACCA GACCATGGGG 
     4321 GAAAAGACTT AAGTCACTGG GTGACAGCTA GTAACAAAGA CAAGAGGACA ACATTAGGTG 
     4381 TTAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//