LOCUS       MR231588                8217 bp    DNA     circular  SYN 
DEFINITION  Asap1 (myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4
ACCESSION   NM_001276463
VERSION     NM_001276463.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4374..4465 
                     /label="Myc-DDK"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    4552..4571 
                     /label="Restriction Enzyme"  
     misc_feature    5356..6028 
                     /label="ColE1"  
     misc_feature    6458..7252 
                     /label="Kan/Neo"  
     misc_feature    7287..7589 
                     /label="SV40 ori"  
     misc_feature    7675..8152 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4356..4362 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4356 
                     /label="Asap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGACCAG ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT 
     1081 ACAACTCGCC CACCACGTCC AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA 
     1141 CGCTGCTGGA GGAGGCTCTA GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG 
     1201 TAAAAGCAAT ATATAATTCC GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG 
     1261 TTCTGGATAA GTTTGGAAGT AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT 
     1321 TTGTCAAGTT TTCTACACTC ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG 
     1381 GTCTGAGCCA CAATGTGATC TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG 
     1441 TCAAAGGAGA TCTCAAGAAG CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA 
     1501 CAAAAATTGA GAAGGAGAAA AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA 
     1561 TAACAGGCGC TGAGATTGCA GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA 
     1621 TGTGTGAATA TCTCATTAAA GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC 
     1681 AGAACCTTAT AAAGTATTAC CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG 
     1741 CTGATAAACT GAAACAGTAC ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA 
     1801 CCCAAGATGA AGAAAAAAAA CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC 
     1861 AACTTGATCC GAAAGAAGAC TCTCAGAGCC GGCAAGGTGG GTACAGCATG CATCAGCTCC 
     1921 AGGGCAACAA GGAGTACGGC AGTGAAAAGA AGGGCTTCCT GCTGAAGAAG AGTGATGGGA 
     1981 TCCGGAAAGT GTGGCAGAGA CGGAAGTGTG CGGTCAAGAA CGGGATCCTG ACCATCTCCC 
     2041 ACGCAACTTC CAACAGGCAG CCTGCTAAGT TAAACCTCCT CACCTGCCAA GTGAAACCGA 
     2101 ATGCTGAGGA CAAGAAGTCT TTTGACCTGA TATCACATAA TCGAACATAT CACTTTCAAG 
     2161 CAGAAGATGA GCAGGATTAT ATAGCGTGGA TATCAGTACT GACGAATAGC AAAGAAGAGG 
     2221 CCCTAACCAT GGCCTTCCGT GGTGAGCAGA GCACAGGGGA GAATAGCCTG GAGGACCTTA 
     2281 CCAAAGCCAT CATCGAGGAT GTACAGCGGC TCCCTGGGAA TGACATCTGC TGTGACTGCG 
     2341 GCTCATCAGA ACCCACGTGG CTTTCAACCA ACTTGGGTAT TTTGACCTGT ATAGAATGTT 
     2401 CCGGAATCCA TAGGGAAATG GGGGTTCATA TTTCTCGCAT TCAGTCTTTG GAACTAGACA 
     2461 AATTAGGAAC TTCCGAACTC TTGCTGGCCA AGAATGTAGG AAACAATAGT TTTAATGATA 
     2521 TTATGGAAGC AAATTTACCC AGCCCTTCAC CAAAGCCCAC CCCTTCAAGT GATATGACCG 
     2581 TGCGTAAAGA GTACATCACT GCAAAGTATG TCGACCACCG CTTCTCCAGG AAGACCTGTG 
     2641 CCTCTTCCTC AGCAAAGCTG AACGAATTGC TTGAGGCCAT CAAGTCCAGG GACTTACTTG 
     2701 CATTAATCCA AGTCTATGCA GAGGGGGTGG AGTTAATGGA GCCACTGCTG GAACCTGGGC 
     2761 AGGAGCTCGG GGAGACAGCC CTTCATCTTG CCGTCAGGAC CGCAGACCAG ACATCTCTCC 
     2821 ATCTGGTTGA TTTCCTTGTA CAAAACTGTG GGAACCTGGA TAAGCAAACG TCTGTGGGGA 
     2881 ATACAGTTCT GCACTATTGC AGCATGTACG GCAAGCCGGA GTGTCTGAAG CTGCTGCTTA 
     2941 GGAGCAAGCC CACCGTGGAC ATCGTTAACC AGAATGGAGA AACTGCCTTG GATATAGCAA 
     3001 AGAGACTCAA AGCTACCCAG TGTGAAGATC TGCTTTCCCA GGCTAAATCT GGAAAGTTCA 
     3061 ACCCTCATGT CCACGTAGAG TATGAGTGGA ATCTTCGACA GGACGAGATG GATGAGAGCG 
     3121 ATGACGATCT GGATGACAAG CCAAGCCCGA TCAAAAAGGA ACGCTCGCCC AGACCACAGA 
     3181 GCTTCTGCCA CTCCTCCAGC ATCTCGCCAC AGGACAAGCT GGCACTGCCA GGGTTCAGCA 
     3241 CTCCACGGGA CAAGCAGCGG CTCTCCTACG GAGCCTTCAC CAACCAGATC TTCGCCTCTA 
     3301 CGAGCACAGA TTTGCCCACA TCACCCACCA GTGAGGCTCC CCCTTTGCCA CCTCGGAACG 
     3361 CCGGGAAAGG TCCAACTGGC CCACCTTCAA CACTCCCTCT AGGCACCCAG ACCTCTAGTG 
     3421 GCAGCTCCAC CCTATCCAAG AAGAGGCCTC CTCCCCCACC ACCAGGACAC AAGAGAACCC 
     3481 TGTCTGACCC TCCCAGCCCA CTACCTCACG GGCCCCCAAA CAAAGGCGCA ATTCCTTGGG 
     3541 GTAATGATGT GGGCCCATTA TCTTCAAGTA AGACGGCCAA CAAGTTTGAG GGGCTGTCTC 
     3601 AGCAAGCAAG CACCAGTTCT GCTAAGACTG CCCTTGGCCC GAGAGTGCTT CCTAAACTAC 
     3661 CTCAGAAAGT GGCACTAAGG AAGACGGAGA CCAGCCATCA TCTCTCCCTC GACAGAACCA 
     3721 ACATCCCACC TGAGACTTTT CAGAAATCAT CACAGTTGAC AGAGTTACCC CAAAAGCCAC 
     3781 CACTTGGAGA GCTGCCCCCG AAGCCTGTGG AACTGGCCCC CAAGCCCCAA GTTGGAGAGC 
     3841 TGCCACCTAA GCCTGGAGAG CTACCCCCTA AGCCCCAATT AGGTGACCTG CCCCCCAAGC 
     3901 CACAGCTCTC AGACTTACCT CCCAAGCCAC AGATGAAGGA CCTGCCCCCT AAGCCGCAGC 
     3961 TGGGGGATCT GCTGGCAAAG TCCCAGGCTG GCGATGTCTC AGCCAAGGTG CAGCCACCCT 
     4021 CAGAGGTCAC ACAGAGGTCA CACACCGGGG ATCTGTCTCC AAATGTACAG TCCAGAGATG 
     4081 CCATCCAGAA GCAAGCATCT GAAGACTCCA ACGACCTCAC ACCCACGCTG CCAGAGACAC 
     4141 CCGTACCACT GCCGAGAAAA ATCAATACGG GGAAAAATAA AGTGAGGCGG GTGAAGACCA 
     4201 TTTATGACTG CCAGGCAGAT AATGACGACG AACTCACATT TATTGAGGGG GAAGTGATCA 
     4261 TTGTCACCGG GGAAGAGGAC CAGGAGTGGT GGATCGGGCA TATCGAAGGA CAGCCTGAAA 
     4321 GGAAGGGTGT CTTTCCAGTG TCCTTTGTCC ACATCCTGTC TGACACGCGT ACGCGGCCGC 
     4381 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4441 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4501 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4561 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4621 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4681 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4741 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4801 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4861 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4921 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4981 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5041 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5101 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5161 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5221 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5281 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5341 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5401 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5461 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5521 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5581 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5641 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5701 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5761 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5821 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5881 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5941 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6001 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6061 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6121 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6181 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6241 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6301 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6361 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6421 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6481 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6541 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6601 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6661 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6721 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6781 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6841 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6901 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6961 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7021 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7081 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7141 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7201 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7261 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7321 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7381 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7441 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7621 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7681 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7741 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7801 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7861 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7921 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7981 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8041 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8101 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8161 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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