LOCUS       MR231575                8160 bp    DNA     circular  SYN 
DEFINITION  Gak (myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak), transcript variant 3
ACCESSION   NM_001282052
VERSION     NM_001282052.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4317..4408 
                     /label="Myc-DDK"  
     misc_feature    4438..5023 
                     /label="PolyA signal"  
     misc_feature    4495..4514 
                     /label="Restriction Enzyme"  
     misc_feature    5299..5971 
                     /label="ColE1"  
     misc_feature    6401..7195 
                     /label="Kan/Neo"  
     misc_feature    7230..7532 
                     /label="SV40 ori"  
     misc_feature    7618..8095 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4299..4305 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4299 
                     /label="Gak"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGAGGAA GAGATCACAA GGAACACCAC ACCCATGTAC AGAACACCAG 
     1081 AAATTGTAGA CCTGTATTCC AACTTCCCCA TTGGCGAAAA GCAGGATATC TGGGCACTGG 
     1141 GCTGTATCTT ATACCTGCTG TGTTTCCGGC AGCATCCTTT TGAGGATGGA GCAAAACTTC 
     1201 GGATAGTCAA TGGGAAGTAT TCCATTCCTG TGAATGACAC TCGTTACACA GTCTTCCATG 
     1261 ACCTTATTCG TGCCATGCTA AAGGTCAACC CAGAGGAGAG GCTGTCCATT GCTGAAGTGG 
     1321 TCCGACAACT GCAGGAAATT GCAGCAGCTA GGAATGTGAA CCCCAAAGCT CCCATCACAG 
     1381 AGCTGCTGGA GCAGAATGGT GGCTATGGGA ACTCAGGGCC TTCCCGAGCA CAACCACCTT 
     1441 GCGGGGGCAC CGTGAACAGC AGTGGAGTTT TGGCTCTGGC AGAGTATGAC CAGCCCTACG 
     1501 GTGGGTTTCT TGATATTCTG CGGGGTGGGA CCGAACGGCT CTTCACCAAC CTCAAGGATA 
     1561 CTTCCTCCAA GGTCATCCAG TCTGTGGCTA ACTATGCAAA GGGTGATCTG GACATATCTT 
     1621 ACATCACATC CAGGATTGCA GTGATGTCAT TCCCAGCAGA AGGTGTGGAG TCTGCAATCA 
     1681 AAAACAATAT AGAGGATGTG CGAATGTTTC TGGATGCTAA GCATCCAGGA CATTATGCTG 
     1741 TCTACAACCT TTCTCCAAGG ATATACCGGG CTTCCAAGTT TCACAACCGG GTCACTGAGT 
     1801 GTGGCTGGGC AGTCAGACGG GCACCACATC TCCACAGTTT GTATACTCTA TGTAGGAGCA 
     1861 TGCATGCCTG GCTCCGGGAA GACCACAGGA ACGTCTGTGT TGTGCATTGC ATGGATGGGA 
     1921 GAGCTGCATC TGCTGTGGCA GTCTGTGCAT TCCTGTGCTT CTGCCGTCTC TTCAGCACTG 
     1981 CAGAGGCTGC TGTGTACATG TTCAGCATGA AGCGCTGCCC ACCAGGCATT TGGCCATCCC 
     2041 ACAAAAGGTA CATTGAATAT GTATGTGACA TGGTGGCAGA GGAGCCCATC ACGCCCCATA 
     2101 GCAAGCCAAT GCTGGTGAAA TCTGTTGTCA TGACCCCCGT GCCACTGTTC AGCAAGCAGA 
     2161 GGAATGGCTG CCGACCGTTC TGTGAGGTCT ACGTTGGAGA GGAGCGTGTC ACCACCACGT 
     2221 CGCAGGAATA TGACAGAATG AAGGAATTTA AAATTGAGGA TGGCAAGGCT GTCATCCCCC 
     2281 TGGGCGTAAC AGTTCAAGGA GACGTGCTCA TCATCATCTA CCATGCCAGG GCCACACTGG 
     2341 GAGGGAGGCT GCAGGCTAAG ATGGCGTCCA TGAAAATGTT CCAGATCCAG TTCCACACTG 
     2401 GGTTCGTGCC TCGAAACGCA ACCACTGTGA AATTTGCAAA ATATGACCTG GATGCTTGTG 
     2461 ATATTCAAGA GAAGTACCCA GATCTGTTCC AGGTGAACCT GGAAGTGGAG GTAGAGCCTA 
     2521 GAGACAGGCC CAGCCGAGAA GCTCCACCTT GGGAGAACAC CAGCCTAAGG GGGTTAAACC 
     2581 CCAAGATCCT CTTTTCCAAC AGGGAAGAGC AGCAGGACAT TCTGTCTAAG TTTGGGAAGC 
     2641 CGGAGCTACC CCGGCAGCCG GGCTCCACAG CTCAGTATGA TGCTGAGGCA GGGTCTCCGG 
     2701 AGGCTGAGAT CACAGAGTCG GACTCACCGC AGAGCAGCAG TACGGACACC AACCACTTTC 
     2761 TTCACACACT GGATTGGCAG GAGGAAAAAG AACCAGAGAC TGGGTTAGAC AATACCTCTC 
     2821 CTAAGGAGAG TCAGTCTGTC CTGATTGCAG ACGGAGATGG AAGTGAAGTA TCAGATGAAG 
     2881 AAGAGGCTTC ATTCCCCAGT GAGGAGAGGA AACCAGGAGC TGGAGAAGAT ACACCAAGGC 
     2941 TGGCTGCTGG GACCAAACAG CAAGACTTAA TATTTGATGT GGGCATGCTG GCTGCCCCAC 
     3001 AGGAGCCTGT ACAGCCCGAA GAAGGTGTTG ATCTCCTGGG GCTACACTCT GAGGGGGACT 
     3061 TAAGGCCTGC TGCCCCCTTA CAGGCTTGTG GGGTCCCATC TAGCAACACT GACCTGTTGA 
     3121 GCTGCCTTCT TGAACCATCT GATGCTGCTC AAGTAGGGCC TCCTGGTGAC CTGCTTGGTG 
     3181 GTGAGGCTCC ACTGCTGCTA GCAAGCCCAG TTTCTCCTCT TGGGCTGCAG AACAACCTAC 
     3241 AAGGAAAAGT CCCTGACACT GTGGACCCAT TTGACCAGTT CCTGCTGTCA TCCAACTCAG 
     3301 ACACCCAGCC CTGCTCCAAG CCTGATCTCT TTGGAGAGTT TCTCAACTCT GACTCTGTAG 
     3361 CTTCCTCAAC TGCCTTCCCA TCCACCCACA GTGCCCCACC CCCATCCTGT AGCACTGCCT 
     3421 TCCTGCACCT AGGGGATCTG CCAGCAGAGC CCAGCAAGGT AATAGCTTCA TCCAGCCACC 
     3481 CAGATCTGCT AGGAGGATGG GATACGTGGG CTGACACAGC TACACCTGGG CCAGCCTCCA 
     3541 TTCCAGTACC AGAAGGTACC CTCTTCTCTT CTGCAGGTCA CCCAGCCCCT CCTGGCCCCA 
     3601 ACCCCAGCCA AACCAAGTCT CAGAACCTTG ACCCATTTGC TGATCTCAGT GATCTCAGCT 
     3661 CTAGCCTCCA AGGCCTGCCT GCTGGACTTC CTGCAGGGGG CTTTGTTGGC GCACCAGCCC 
     3721 CCACTCAAAA GAGCAACAGC CCCTGGCAGG CAAATCGTCC CACAGCCCCT GGAACCTCAT 
     3781 GGACCCCCCA GGCCAAGCCA GCCCCCAGAG CCTCTGAACA GCTAAGGTCT CACTTCAGTG 
     3841 TGATCGGGGC CCGAGAAGAG AGGGGCGTCC GTGTGCCCAG CTTTGCCCAA AAGCCAAAGG 
     3901 TCTCAGAAAA TGATTTTGAA GATCTTCTGC CTAATCAAGG CTTCTCTAAG TCTGACAAGA 
     3961 AGGGGCCAAA GACCATGGCA GAGATGCGGA AACAGGAACT TGCCAGAGAT ACAGACCCAC 
     4021 TCAAATTGAA GCTTTTGGAC TGGATTGAAG GCAAGGAGAG GAACATTCGT GCACTGCTGT 
     4081 CCACTCTGCA CACGGTATTG TGGGATGGGG AGAGCCGCTG GACACCTGTG AGTATGGCTG 
     4141 ACCTGGTGAC TCCAGAGCAG GTGAAGAAGC AGTACCGCCG TGCAGTGCTG GTAGTGCATC 
     4201 CTGATAAGGC CACAGGGCAG CCGTATGAAC AGTATGCCAA GATGATCTTC ATGGAGCTGA 
     4261 ATGATGCATG GTCTGAGTTT GAAAACCAGG GCTCGAGGCC CCTCTTCACG CGTACGCGGC 
     4321 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4381 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5401 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5641 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5941 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6001 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6061 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6121 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6181 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6241 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6301 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6361 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6421 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6481 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6541 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6601 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6661 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6721 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6781 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6841 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6901 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6961 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7021 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7081 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7141 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7201 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7261 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7321 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7381 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7561 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7621 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7681 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7741 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7801 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7861 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7921 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7981 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8041 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8101 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//