LOCUS       MR231557                8115 bp    DNA     circular  SYN 
DEFINITION  Myo1b (myc-DDK-tagged) - Mouse myosin IB (Myo1b), transcript variant 3
ACCESSION   NM_001290982
VERSION     NM_001290982.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4272..4363 
                     /label="Myc-DDK"  
     misc_feature    4393..4978 
                     /label="PolyA signal"  
     misc_feature    4450..4469 
                     /label="Restriction Enzyme"  
     misc_feature    5254..5926 
                     /label="ColE1"  
     misc_feature    6356..7150 
                     /label="Kan/Neo"  
     misc_feature    7185..7487 
                     /label="SV40 ori"  
     misc_feature    7573..8050 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4254..4260 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4254 
                     /label="Myo1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAAATG GAGGTGAAAT CCTCACTTCT GGACAATATG ATCGGTGTTG 
     1081 GAGATATGGT TCTTTTAGAG CCGCTCAATG AGGAGACGTT CATCGACAAC CTGAAGAAGC 
     1141 GCTTCGACCA CAATGAGATA TATACATACA TTGGGAGCGT GGTTATATCT GTTAACCCCT 
     1201 ACCGGTCCTT GCCCATATAT TCGCCGGAGA AAGTGGAAGA TTACAGAAAT AGAAACTTCT 
     1261 ATGAACTGAG CCCTCACATC TTTGCTTTGT CGGATGAAGC ATACAGATCC CTCCGTGATC 
     1321 AAGATAAAGA TCAATGCATT CTCATTACTG GGGAGAGTGG AGCAGGAAAA ACAGAGGCCA 
     1381 GCAAGCTCGT CATGTCCTAT GTCGCTGCCG TGTGCGGAAA AGGAGCAGAA GTCAATCAGG 
     1441 TCAAGGAACA GCTTCTGCAG TCCAACCCTG TCCTGGAAGC TTTTGGAAAT GCCAAGACTG 
     1501 TGAGGAATGA CAACTCATCT AGATTCGGCA AATACATGGA TATTGAATTT GACTTTAAAG 
     1561 GCGATCCGCT GGGAGGAGTG ATAAGTAACT ATCTTTTAGA GAAATCTCGT GTTGTTAAGC 
     1621 AGCCAAGAGG TGAAAGAAAC TTCCATGTGT TCTATCAGCT GCTCTCAGGC GCCTCTGAGG 
     1681 AGCTCCTCTA CAAGCTTAAG CTGGAGCGGG ATTTCAGCAG ATACAACTAT CTGAGTCTGG 
     1741 ACTCTGCTAA AGTTAATGGT GTGGATGATG CTGCCAATTT CAGAACTGTT AGGAATGCGA 
     1801 TGCAGATCGT GGGCTTTCTG GATCATGAGG CCGAGGCTGT CCTGGAGGTA GTGGCAGCCG 
     1861 TTTTGAAACT GGGGAACATC GAGTTCAAGC CTGAATCACG AGTGAATGGT TTGGATGAAA 
     1921 GCAAAATCAA AGATAAAAAT GAGTTAAAAG AAATTTGCGA GTTGACCAGC ATCGATCAGG 
     1981 TGGTTTTAGA AAGAGCGTTC AGTTTCCGAA CAGTTGAAGC GAAGCAGGAG AAAGTTTCAA 
     2041 CCACGCTGAA TGTGGCTCAG GCTTATTATG CCCGTGATGC CCTGGCTAAA AACCTCTACA 
     2101 GCCGCTTGTT TTCATGGTTG GTAAATCGTA TCAACGAAAG CATTAAGGCA CAAACAAAAG 
     2161 TGAGAAAGAA GGTCATGGGT GTTCTGGACA TTTATGGCTT TGAAATATTT GAGGATAACA 
     2221 GCTTCGAGCA GTTTATTATT AACTATTGTA ATGAAAAGCT TCAACAAATC TTCATCGAAC 
     2281 TTACCCTCAA AGAAGAGCAA GAGGAATATA TTCGGGAGGA CATAGAATGG ACTCACATTG 
     2341 ACTACTTCAA CAATGCTATC ATTTGTGACC TAATAGAAAA TAACACAAAT GGAATCTTGG 
     2401 CCATGCTGGA TGAAGAGTGC CTGAGACCCG GCACTGTTAC AGATGAGACC TTCCTGGAAA 
     2461 AGCTGAACCA AGTCTGTGCC ACCCACCAGC ACTTTGAGAG CAGGATGAGC AAGTGTTCCC 
     2521 GCTTTCTCAA CGACACAACC CTGCCCCACA GCTGCTTCAG AATCCAGCAT TATGCTGGCA 
     2581 AGGTGCTCTA CCAGGTGGAA GGATTTGTTG ACAAGAACAA TGACCTTCTG TACCGGGACC 
     2641 TGTCTCAAGC CATGTGGAAG GCCGGCCACT CCCTCATCAA GTCTCTGTTT CCTGAAGGAA 
     2701 ATCCTGCAAA GGTCAACCTG AAGAGACCTC CCACCGCAGG CTCCCAGTTC AAGGCGTCTG 
     2761 TGGCCACGCT GATGAGAAAC CTGCAGACCA AGAACCCGAA CTACATCAGG TGCATCAAGC 
     2821 CAAATGATAA AAAGGCAGCT CACATTTTCA ATGAGAGTCT CGTATGCCAT CAGATCAGGT 
     2881 ACCTGGGTCT TTTGGAGAAC GTTCGAGTGA GACGGGCAGG CTATGCCTTC AGGCAGGCCT 
     2941 ATGAACCTTG CCTGGAAAGA TACAAGATGC TTTGCAAACA AACGTGGCCT CACTGGAAGG 
     3001 GACCAGCAAG GTCTGGCGTG GAGGTTCTGT TTAATGAGCT AGAGATTCCT GTGGAAGAGC 
     3061 ACTCCTTCGG GAGATCCAAG ATATTCATCC GAAACCCACG GACATTATTC CAACTAGAAG 
     3121 ACCTAAGGAA GCAGCGGCTG GAGGACCTGG CCACTCTCAT TCAGAAGATT TATCGAGGAT 
     3181 GGAAATGCCG TACACATTTT CTGCTCATGA AAAGAAGCCA AGTTGTGATT GCTGCCTGGT 
     3241 ACAGGAGATA TGCGCAACAA AAGCGGTACC AGCAGATAAA GAGTTCAGCC CTGGTGATTC 
     3301 AGTCGTACAT CCGGGGCTGG AAGGCTCGAA AAATCCTGCG GGAGCTGAAG CATCAGAAGC 
     3361 GCTGTAAGGA GGCAGCCACC ACCATCGCGG CGTACTGGCA TGGCACCCAG GTACGCAGGG 
     3421 AATACAGGAA ATTCTTTAGA GCAAATGCTG GAAAGAAAAT CTATGAGTTT ACACTTCAAA 
     3481 GAATTGTGCA AAAATACTTG TTGGAAATGA AAAATAAAAT GCCTTCCTTG TCACCGATAG 
     3541 ATAAAAATTG GCCATCAAGA CCTTACCTGT TCTTGGATTC GACTCACAAG GAGCTAAAAA 
     3601 GGATCTTCCA CTTGTGGAGG TGTAAAAAAT ACAGGGATCA ATTCACAGAC CAGCAGAAAC 
     3661 TTATTTATGA AGAGAAGCTC GAGGCCAGCG AACTCTTCAA AGACAAGAAG GCTTTATATC 
     3721 CTTCTAGTGT TGGGCAACCA TTCCAAGGAG CTTACCTGGA AATCAACAAG AACCCAAAAT 
     3781 ATAAGAAACT CAAAGATGCC ATTGAAGAGA AGATCATTAT TGCTGAGGTT GTGAACAAAA 
     3841 TTAATCGGGC TAATGGGAAG AGTACATCTC GGATTTTCCT CTTAACAAAC AATAACCTTC 
     3901 TCCTTGCTGA CCAAAAGTCT GGGCAGATCA AGTCCGAGGT CCCCCTGGTA GATGTGACCA 
     3961 AGGTTTCCAT GAGTTCCCAG AACGATGGTT TCTTTGCAGT GCACCTCAAG GAGGGTTCAG 
     4021 AAGCGGCTAG TAAAGGGGAC TTTCTCTTCA GCAGTGACCA CCTGATTGAA ATGGCAACCA 
     4081 AGCTGTATCG CACGACTCTC AGCCAAACTA AACAGAAGCT CAACATCGAG ATTTCCGATG 
     4141 AGTTCCTGGT ACAGTTCAGA CAGGACAAAG TATGTGTGAA GTTTATTCAA GGCAACCAGA 
     4201 AAAATGGGAG CGTGCCAACC TGCAAACGAA AGAACAACAG ACTCCTTGAA GTCGCTGTCC 
     4261 CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4321 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5041 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5101 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5161 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5221 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5281 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5341 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5401 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5461 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5521 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5581 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5641 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5701 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5761 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5821 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5881 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5941 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6001 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6061 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6121 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6181 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6241 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6301 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6361 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6421 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6481 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6541 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6601 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6661 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6721 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6781 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6841 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6901 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6961 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7021 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7081 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7141 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7201 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7261 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7321 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7381 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7441 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7501 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7561 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7621 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7681 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7741 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7801 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7861 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7921 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7981 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8041 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8101 TTTAACGCGA ATTTT
//