LOCUS       MR231555                8112 bp    DNA     circular  SYN 
DEFINITION  Daam1 (myc-DDK-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 2
ACCESSION   NM_172464
VERSION     NM_172464.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4269..4360 
                     /label="Myc-DDK"  
     misc_feature    4390..4975 
                     /label="PolyA signal"  
     misc_feature    4447..4466 
                     /label="Restriction Enzyme"  
     misc_feature    5251..5923 
                     /label="ColE1"  
     misc_feature    6353..7147 
                     /label="Kan/Neo"  
     misc_feature    7182..7484 
                     /label="SV40 ori"  
     misc_feature    7570..8047 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4251..4257 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4251 
                     /label="Daam1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCACGG AAGAGAGGCG GCCGGGGGAT TTCATTTATC TTTTGCTGTT 
     1081 TCCGAAACAA TGACCATCCA GAAATCACAT ATCGGCTTAG GAATGATAGC AACTTTGCAC 
     1141 TTCAGACAAT GGAGCCGGCG CTGCCCATGC CCCCTGTGGA GGAGCTCGAT GTCATGTTCA 
     1201 GTGAACTTGT GGATGAACTT GACCTCACAG ACAAACACAG GGAAGCCATG TTTGCACTTC 
     1261 CAGCAGAGAA AAAGTGGCAG ATATACTGTA GCAAGAAAAA GGACCAGGAA GAAAACAAGG 
     1321 GAGCAACCAG TTGGCCCGAG TTCTACATTG ATCAGCTCAA TTCCATGGCT GCTAGGAAAT 
     1381 CTCTGCTGGC TTTAGAGAAG GAAGAAGAGG AGGAGAGGAG TAAAACCATC GAGAGTCTGA 
     1441 AGACAGCACT GAGAACCAAG CCCATGAGGT TTGTAACCAG ATTCATCGAC TTGGATGGCC 
     1501 TGTCATGTAT TCTCAACTTT CTGAAGACCA TGGACTATGA GACCTCTGAG TCTCGGATAC 
     1561 ACACCTCCCT CATTGGCTGT ATAAAGGCAC TAATGAACAA CTCTCAAGGC CGGGCCCATG 
     1621 TCCTGGCTCA TTCTGAGAGT ATCAATGTAA TTGCTCAGAG TCTGAGCACA GAGAACATTA 
     1681 AAACAAAGGT GGCCGTGCTG GAAATCCTGG GCGCTGTGTG CCTGGTTCCT GGGGGCCACA 
     1741 AGAAAGTTCT GCAAGCTATG CTGCACTACC AGAAGTATGC CAGCGAGAGA ACCCGCTTCC 
     1801 AGACGTTAAT TAATGACTTG GATAAAAGCA CTGGGCGTTA TCGGGATGAA GTAAGTCTCA 
     1861 AGACGGCCAT CATGTCCTTC ATCAATGCAG TGCTGAGCCA GGGAGCCGGA GTGGAGAGTT 
     1921 TGGACTTCAG GCTCCATCTT CGCTATGAAT TTCTGATGTT AGGAATTCAA CCTGTTATAG 
     1981 ATAAATTAAG GGAACATGAA AATTCAACAC TAGACAGGCA TTTAGACTTT TTTGAAATGC 
     2041 TCCGAAATGA AGATGAACTA GAATTTGCCA AAAGATTTGA ACTGGTTCAC ATAGACACAA 
     2101 AAAGCGCCAC TCAGATGTTT GAGCTGACCA GGAGGAGGCT AACCCACAGC GAAGCCTACC 
     2161 CCCACTTCAT GTCCATTCTG CACCATTGCC TCCAGATGCC TTACAAGAGG AGTGGTAACA 
     2221 CAGTTCAGTA CTGGCTGCTG TTAGACAGAA TCATACAGCA GATAGTTATC CAGAATGACA 
     2281 AAGGGCAGGA TCCCGACTCC ACACCTCTGG AAAACTTTAA TATCAAGAAC GTCGTAAGAA 
     2341 TGTTGGTTAA TGAAAATGAA GTTAAGCAAT GGAAAGAGCA GGCAGAGAAG ATGAGGAAAG 
     2401 AGCACAATGA GCTACAACAG AAGCTGGAGA AGAAAGAGAG AGAATGTGAT GCTAAGACCC 
     2461 AAGAGAAGGA AGAAATGATG CAGACCTTAA ACAAGATGAA AGAGAAGCTG GAAAAGGAGA 
     2521 CAACTGAGCA CAAGCAAGTT AAGCAGCAGG TGGCAGACCT CACGGCGCAG CTCCACGAGC 
     2581 TGAACAGGAG GGCCGTCTGT GCAGCAGTCC CAGGAGGACC CTCACCCGGA GCCCCTGGAG 
     2641 GACCCTTTCC TTCCTCTGGG TTGGGGTCTC TTCTCCCTCC CCCACCACCC CCACTCCTTT 
     2701 CAGGTGGAGC ACTTCCTCCT CCACCACCTC CCCTCCCTCC TGGGGGTCCT CCTCCTCCCC 
     2761 CAGGGCCTCC TCCCTTAGGA GGAGTTCTGC CACCTCCTGG TGCTCCAGTG AGTCTAACAC 
     2821 TCAAGAAGAA GAATATTCCC CAGCCCACCA ATGCCCTGAA ATCCTTCAAC TGGTCCAAGT 
     2881 TGCCTGAGAA CAAATTGGAT GGAACAGTAT GGACTGAAAT TGATGATACC AAAGTCTTCA 
     2941 AAATTCTAGA CCTTGAAGAC CTAGAAAGAA CGTTCTCTGC CTACCAAAGA CAGCAGGAGT 
     3001 TCTTTGTGAA CAACTCCAAG CAGAAAGAAG CAGATGCCAT TGATGACACA CTGAGTTCCA 
     3061 AACTTAAAGT CAAAGAGCTG TCAGTGATTG ATGGTCGGAG AGCTCAGAAT TGCAACATCC 
     3121 TTCTGTCGAG GTTGAAATTA TCCAATGATG AAATCAAGCG GGCAATTCTA ACAATGGATG 
     3181 AACAGGAGGA TCTGCCCAAG GACATGTTAG AACAGCTTTT GAAATTCGTT CCTGAGAAAA 
     3241 GCGACATCGA CCTGCTAGAG GAACACAAGC ATGAGCTGGA TCGGATGGCC AAGGCTGACC 
     3301 GCTTCCTGTT TGAGATGAGC AGAATTAATC ATTACCAGCA AAGACTGCAA TCACTGTACT 
     3361 TCAAAAAGAA GTTTGCAGAG CGTGTGGCAG AAGTGAAGCC CAAAGTAGAA GCGATTCGCT 
     3421 CTGGCTCTGA GGAGGTGTTC AGGAGCCGCG CCCTCAAGCA GCTCCTGGAG GTGGTCTTGG 
     3481 CTTTTGGAAA TTATATGAAT AAAGGACAAA GAGGCAATGC ATATGGATTC AAGATCTCCA 
     3541 GCCTCAACAA GATAGCGGAT ACCAAATCCA GTATTGACAA GAACATTACT CTTTTGCACT 
     3601 ATCTAATAAC TATTGTGGAG AATAAGTACC CCAAAGTCCT CAACCTAAGC GAAGAGTTGC 
     3661 GGGATATTCC TCAAGCAGCG AAAGTAAACA TGACTGAGCT GGACAAGGAG ATCAGCACCC 
     3721 TGAGGAGTGG CCTGAAAGCT GTAGAGACGG AGCTGGAGTA TCAGAAGTCT CAGCCCCCAC 
     3781 AGCCTGGAGA CAAGTTCGTG TCTGTTGTCA GCCAGTTCAT CACCCTGGCT AGCTTCAGCT 
     3841 TCTCAGATGT TGAAGATCTT CTAGCAGAAG CTAAAGAGCT GTTCACTAAA GCAGTGAAGC 
     3901 ACTTTGGGGA AGAGGCTGGC AAGATCCAGC CAGATGAGTT CTTCGGGATC TTTGATCAGT 
     3961 TTCTTCAGGC CGTGGCCGAA GCGAAACAGG AAAATGAGAA CATGAGAAAA AGGAAGGAGG 
     4021 AGGAGGAACG GCGGGCTCGC CTGGAAGCTC AGCTCAAAGA GCAGCGGGAG AGGGAGCGGA 
     4081 AGGTGAGGAA GGCCAAGGAG AGCAGTGAAG AGAGTGGAGA GTTTGACGAC CTGGTCTCGG 
     4141 CGCTGCGCTC AGGAGAGGTG TTCGACAAAG ACCTTTCAAA ACTCAAACGG AACCGGAAAC 
     4201 GCATTTCCAA CCAGGTGACG GACAGCAGCC GGGAGCGACC AATCACAAAA CTTAATTTTA 
     4261 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4321 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4381 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4441 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4501 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4561 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4621 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4681 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4741 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4801 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4861 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4921 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4981 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5041 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5101 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5161 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5221 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5281 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5341 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5401 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5461 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5521 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5581 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5641 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5701 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5761 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5821 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5881 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5941 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6001 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6061 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6121 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6181 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6241 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6301 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6361 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6421 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6481 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6541 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6601 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6661 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6721 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6781 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6841 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6901 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6961 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7021 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7081 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7141 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7201 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7261 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7321 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7381 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7561 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7621 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7681 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7741 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7801 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7861 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7921 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7981 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8041 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8101 AACGCGAATT TT
//