LOCUS       MR231544                8088 bp    DNA     circular  SYN 
DEFINITION  Sema6d (myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (Sema6d), transcript variant 7
ACCESSION   NM_001291000
VERSION     NM_001291000.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4245..4336 
                     /label="Myc-DDK"  
     misc_feature    4366..4951 
                     /label="PolyA signal"  
     misc_feature    4423..4442 
                     /label="Restriction Enzyme"  
     misc_feature    5227..5899 
                     /label="ColE1"  
     misc_feature    6329..7123 
                     /label="Kan/Neo"  
     misc_feature    7158..7460 
                     /label="SV40 ori"  
     misc_feature    7546..8023 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4227..4233 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4227 
                     /label="Sema6d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTTCCTT CTGCTTTGGT TCTGCGTGCT GTTCCTTCTG GTCTCCAGGT 
     1081 TACGGGCGGT CAGCTTCCCA GAAGACGATG AGCCCCTCAA CACGGTTGAC TATCACTATT 
     1141 CAAGGCAATA TCCGGTTTTT AGAGGACGCC CTTCAGGCAA CGAATCGCAG CACAGGCTGG 
     1201 ACTTTCAGCT GATGTTGAAA ATTCGAGACA CACTTTATAT TGCTGGCAGG GATCAAGTCT 
     1261 ATACAGTGAA CTTAAATGAA ATCCCCCAAA CAGAGGTGAT ACCAAGCAAG AAGCTGACGT 
     1321 GGAGGTCCAG ACAGCAGGAT CGAGAAAATT GTGCTATGAA AGGCAAGCAT AAAGATGAAT 
     1381 GCCACAACTT CATCAAAGTC TTTGTCCCAA GAAATGATGA GATGGTTTTT GTCTGTGGTA 
     1441 CCAATGCTTT CAACCCGATG TGCAGATACT ATAGGTTGAG AACGTTAGAG TATGATGGGG 
     1501 AAGAAATTAG TGGCCTGGCA CGATGCCCGT TTGATGCCCG ACAAACCAAT GTCGCCCTCT 
     1561 TTGCTGATGG AAAACTCTAT TCTGCCACAG TGGCTGATTT CCTGGCCAGT GATGCTGTCA 
     1621 TTTACAGAAG CATGGGAGAT GGATCTGCCC TTCGCACAAT AAAATACGAT TCCAAGTGGA 
     1681 TCAAAGAACC ACACTTCCTT CATGCCATAG AATATGGAAA CTATGTCTAT TTCTTCTTCA 
     1741 GAGAAATCGC CGTGGAACAT AATAACTTAG GCAAGGCTGT GTATTCCCGC GTGGCTCGCA 
     1801 TTTGTAAAAA CGACATGGGT GGCTCACAGC GGGTCCTGGA GAAACACTGG ACTTCCTTCC 
     1861 TTAAGGCTCG GCTGAACTGC TCCGTTCCTG GAGATTCCTT TTTCTACTTC GACGTCCTGC 
     1921 AGTCTATAAC AGACATAATC CAAATCAATG GCATCCCCAC TGTGGTTGGG GTCTTCACCA 
     1981 CACAGCTCAA CAGCATTCCT GGTTCTGCAG TCTGTGCCTT TAGCATGGAC GACATTGAGA 
     2041 AAGTGTTCAA AGGGCGGTTC AAAGAGCAGA AAACCCCAGA CTCTGTTTGG ACAGCAGTTC 
     2101 CCGAAGACAA AGTACCAAAA CCAAGGCCTG GCTGTTGTGC CAAACACGGC CTCGCAGAAG 
     2161 CTTACAAGAC CTCCATCGAC TTTCCAGATG ACACCCTGGC TTTCATCAAG TCCCACCCGC 
     2221 TGATGGACTC TGCCGTCCCA CCCATTGCCG ATGAGCCCTG GTTCACAAAG ACACGGGTCA 
     2281 GGTACAGGTT GACAGCCATC GAAGTGGACC GTTCAGCAGG GCCATACCAA AACTACACAG 
     2341 TCATCTTTGT TGGCTCTGAA GCTGGCGTGG TACTTAAAGT TTTGGCAAAG ACCAGTCCTT 
     2401 TCTCTCTGAA TGACAGTGTA TTACTCGAAG AGATTGAAGC TTATAACCCA GCCAAGTGCA 
     2461 GCGCCGAGAG TGAGGAGGAC AGAAAGGTGG TCTCATTACA GCTGGACAAG GATCACCATG 
     2521 CTTTATACGT GGCCTTCTCT AGCTGCGTGG TCCGCATCCC CCTCAGCCGC TGTGAGCGCT 
     2581 ACGGATCGTG TAAAAAGTCT TGCATTGCAT CACGTGACCC GTACTGTGGT TGGTTAAGCC 
     2641 AGGGAGTTTG TGAGAGAGTG ACCCTAGGGA TGCTCCCTGG AGGATATGAG CAGGACACGG 
     2701 AGTACGGCAA CACAGCCCAC CTAGGGGACT GCCACGAAAG TTTGCCTCCT TCAACTACAC 
     2761 CAGATTACAA AATATTTGGC GGTCCAACAT CTGACATGGA GGTATCCTCA TCTTCTGTTA 
     2821 CCACTGTGGC AAGTAGCCCA GAAATTACAT CTAAAGTGAT TGATACCTGG AGACCTAAAC 
     2881 TGACGAGCTC CCGGAAATTT GTAGTTCAAG ATGACCCAAA TACTTCTGAT TTTACTGATA 
     2941 CTATATCAGG TGTACGGTGG GAAGTCCAGT CTGGAGAATC CAATCAGATG GTCCACATGA 
     3001 ATGTCCTCAT CACCTGCGTG TTTGCCGCTT TTGTCTTGGG CGCGTTCATC GCAGGAGTGG 
     3061 CCGTGTACTG CTACCGTGAC ATGTTCGTTC GGAAGAACAG AAAGATCCAT AAAGACGCAG 
     3121 AATCCGCCCA GTCGTGCACA GACTCCAGCG GAAGCTTCGC CAAGCTGAAC GGCCTCTTTG 
     3181 ACAGCCCCGT CAAGGAATAC CAGCAGAACA TTGATTCTCC CAAACTCTAC AGCAACCTGC 
     3241 TGACCAGTCG GAAGGAACTG CCACCAAACA CGGATACAAA GTCCATGGCC GTGGACCACA 
     3301 GAGGCCAGCC TCCCGAGCTG GCTGCTCTCC CCACGCCGGA ATCCACACCT GTCCTCCACC 
     3361 AGAAGACCCT GCAGGCCATG AAGAGCCACT CTGAGAAGGC CCACAGCCAC GGTGCTTCAA 
     3421 GGAAAGAACA CCCCCAGTTT TTTCCTTCTA GTCCTCCACC CCATTCCCCA TTGAGTCACG 
     3481 GGCATATCCC CAGTGCCATC GTTCTTCCAA ACGCCACTCA CGACTACAAT ACATCCTTCT 
     3541 CCAACTCGAA TGCCCACAAA GCCGAAAAGA AGCTTCAGAG CATGGATCAC CCTCTTACGA 
     3601 AGTCATCCAG TAAGCGGGAG CACCGGCGGT CTGTGGATTC CAGGAATACT CTCAATGATC 
     3661 TCCTGAAGCA TCTAAATGAC CCAAACAGTA ACCCCAAAGC CATCCTGGGA GAGATCCATA 
     3721 TGGCTCATCA AACCCTCATG CTGGACCCGG TGGGACCAAT GGCTGAGGTC CCACCCAAGG 
     3781 TCCCTAACCG GGAGGCATCT CTATACTCCC CTCCCTCCAC ACTCCCCAGA AATAGTCCAA 
     3841 CCAAGAGAGT AGATGTCCCC ACCACTCCTG GGGTGCCAAT GACTTCTCTG GAAAGACAAA 
     3901 GGGGTTATCA CAAAAATTCC TCCCAGAGGC ACTCTATATC TGCCGTGCCT AAAAACTTAA 
     3961 ACTCACCAAA TGGTGTTTTG TTATCTAGAC AGCCGAGTAT GAACCGTGGA GGCTATATGC 
     4021 CCACCCCAAC AGGGGCGAAG GTGGACTATA TTCAGGGGAC ACCGGTGAGT GTTCATCTGC 
     4081 AGCCCTCCCT CTCCAGACAG AGCAGCTATA CCAGTAATGG CACCCTCCCC AGGACGGGAC 
     4141 TAAAGAGGAC ACCATCCTTA AAACCTGATG TGCCACCAAA GCCTTCCTTT GTTCCGCAAA 
     4201 CCACATCTGT CAGACCACTG AACAAGTACA CGTACACGCG TACGCGGCCG CTCGAGCAGA 
     4261 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4321 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4381 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4441 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4501 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4561 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4621 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4681 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4741 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4801 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4861 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4921 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4981 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5041 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5101 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5161 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5221 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5281 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5341 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5401 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5461 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5521 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5581 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5641 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5701 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5761 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5821 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5881 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5941 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6001 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6061 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6121 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6181 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6241 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6301 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6361 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6421 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6481 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6541 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6601 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6661 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6721 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6781 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6841 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6901 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6961 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7021 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7081 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7141 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7201 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7261 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7321 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7381 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7501 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7561 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7621 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7681 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7741 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7801 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7861 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7921 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7981 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8041 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//