LOCUS       MR231526                8034 bp    DNA     circular  SYN 
DEFINITION  Llgl2 (myc-DDK-tagged) - Mouse lethal giant larvae homolog 2 (Drosophila) (Llgl2), transcript variant 2
ACCESSION   NM_001252532
VERSION     NM_001252532.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4191..4282 
                     /label="Myc-DDK"  
     misc_feature    4312..4897 
                     /label="PolyA signal"  
     misc_feature    4369..4388 
                     /label="Restriction Enzyme"  
     misc_feature    5173..5845 
                     /label="ColE1"  
     misc_feature    6275..7069 
                     /label="Kan/Neo"  
     misc_feature    7104..7406 
                     /label="SV40 ori"  
     misc_feature    7492..7969 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4173..4179 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4173 
                     /label="Llgl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCGGTTC CTGAGAACTG GACATGACCC TGCCCGGGAG AGGCTGAAGC 
     1081 GGGATCTGTT CCAGTTTAAC AAGACAGTGG AGCATGGCTT CCCACACCAG CCCAGCGCCC 
     1141 TTGGCTATAG CCCTTCGCTG CGCATCCTGG CCATCGGAAC CCGCTCTGGA GCTGTCAAGC 
     1201 TATATGGCGC CCCCGGGGTA GAGTTCATGG GCCTTCACAA GGAGAACAAC GCAGTGCTGC 
     1261 AGATCCACTT CCTGCCTGGT CAGTGTCAGC TGGTCACTCT GCTGGATGAC AATAGCCTGC 
     1321 ACCTGTGGAG CCTGAAGGTC AAGGGCGGGG TGTCAGAGCT GCAGGAAGAA GAGAGCTTCA 
     1381 CATTGCGTGG CCCCCCAGGG GCTGCCCCCA GTGCCACGCA GGTCACTGAG ATTCTACCTC 
     1441 ACTCTTCCGG AGAACTGCTC TACCTGGGCA CCGAGAGCGG CAACGTGTTT GTGGTGCAGC 
     1501 TCCCGGGGTT CCGCACGCTG CACGACAGGA CCATCTGTTC AGATGAGGTG CTGCAATGGT 
     1561 TGCCAGAGGA GGCTCGCCAC CGGCGAGTGT TTGAGATGGT GGAGGCTCTG CAGGAGCACC 
     1621 CTCGAGACCC CAACCAAATC CTCATTGGCT ACAGCCGAGG CCTCGTCGTC ATCTGGGACC 
     1681 TTCAGGGCAG CCGAGCGCTC AGTCATTTCC TCAGCAGCCA GCAACTGGAG AATGCCAGCT 
     1741 GGCAGCGGGA TGGCTGCCTG ATTGTCACCT GCCACTCCGA CGGCAGCCAC TGCCAGTGGC 
     1801 CCGTCTCCAG TGACACCCAG AACCCAGAAC CTCTGCGCAG CTCTATACCT TACGGTCCCT 
     1861 TTCCTTGCAA AGCTATAACG AAAATCTTCT GGCTCACCAC CAGGCAAGGG TTGCCCTTCA 
     1921 CCATCTTCCA GGGCGGTATG CCACGGGCCA GCTATGGGGA CCGCCACTGC ATCTCAGTGG 
     1981 TCCACAACGG CCAGCAGACG GCCTTCGACT TCACCTCCCG TGTCATTGAC TTCACTGTTC 
     2041 TCTCAGAGGC TGACCCTGCG GCTGCCTTTG ATGACCCCTA CGCCCTGGTG GTGCTAGCCG 
     2101 AGGAGGAACT GGTAGTCATT GACCTGCAGA CACCCGGCTG GCCACCAGTC CAGCTGCCCT 
     2161 ACCTGGCTTC CTTGCACTGT TCCGCTATCA CCTGCTCCCA CCACGTCTCC AACATCCCCC 
     2221 TGAAGCTATG GGAACGCATC ATCGCCGCGG GCAGCCGCCA GAACTCACAC TTCTCCACCA 
     2281 TGGAGTGGCC CATAGATGGT GGCACCAGCC TGGCCCCACC GCCACCCCAG AGGGACCTGC 
     2341 TGCTCACAGG GCACGAGGAC GGCACGGTGC GGTTCTGGGA CGCCTCCGGT GTGTGCTTAC 
     2401 GTCTGCTCTA CAAACTGAGC ACTGTGAGGG TGTTCCTCAC AGACACGGAC CCCAGCGAGA 
     2461 ACCTCATTGC CCAGGGTGAG GACGAGTGGC CCCCACTCCG CAAGGTGGGC TCCTTTGATC 
     2521 CCTACAGCGA TGATCCGCGG CTAGGCATCC AGAAGATTTT CCTCTGCAAA TACAGCGGCT 
     2581 ACCTGGCTGT GGCAGGCACG GCAGGGCAGG TGCTGGTGCT GGAGTTGAAC GATGAGGCGG 
     2641 CCGAGCATGC TGTGGAGCAG GTGGAGGCTG ACCTGCTGCA GGACCAGGAG GGTTACCGCT 
     2701 GGAAGGGGCA CGAGCGCCTC GCCGCCCGCC CGGGGCCCGT GTGCTTTGAG GCGGGATTTC 
     2761 AGCCCTTTGT ACTGGTGCAG TGCCAGCCCC CAGCTGTGGT CACCTCCTTG GCTCTGCACT 
     2821 CCGAGTGGCG GCTTGTGGCC TTTGGCACCA GCCATGGTTT CGGCCTCTTC GATCACCAGC 
     2881 AACGGCGGCA GGTCTTTGTC AAGTGCACAC TTCACCCCAG TGACCAGCTG GCCTTGGAGG 
     2941 GCCCTCTGTC TCGAGTAAAG TCCCTCAAGA AGTCTCTACG TCAGTCATTC CGTCGGATGC 
     3001 GTCGCAGCAG AGTGTCCAGT CATAAACGGC GGCCTGGTGG CCCCACAGGC GAGGTGAGGC 
     3061 CCCAGGCTCA GAGCAGCCAT CTACAGGACC ATACAGCTCT CCCTCAGGAT CTGAATCCAG 
     3121 ATCAAGCGCA GGCCCAGGCT GTGAATATCA AGGCTGAGCG GACGGGCCTG CAGAACATGG 
     3181 AGTTGGCGCC CGTGCAACGG AAGATTGAGG CTCGCTCCGC GGAGGACTCC TTCACTGGCT 
     3241 TTGTCCGGAC CCTCTATTTC GCTGATACCT ACCTGAGGGA CAGTTCCCGC CACTGTCCCT 
     3301 CCCTGTGGGC TGGCACCAAC GGAGGCACCG TCTACGCCTT TTCCCTGCGC GTGCCTCCTG 
     3361 CAGAAAGAAG AACAGATGAA CCTGTCCGGG CAGAGCAGGC CAAGGAGATC CAGCTGATGC 
     3421 ATCGCGCGCC TGTGGTGGGC ATCCTGGTGC TCGACGGACA CAACGTGCCC CTCCCCGAGC 
     3481 CCCTGGAAGT CGCCCATGAC CTGTCGAAGA GCCCAGACAT GCAAGGGAGC CACCAATTGC 
     3541 TTGTGGTGTC GGAGGAGCAA TTCAAGGTAT TCACACTGCC CAAGGTGAGC GCCAAACTGA 
     3601 AGCTGAAGCT GACGGCCCTG GAGGGCTCAC GGGTGCGGCG AGTCGGTGTC GCCCACTTTG 
     3661 GCAGCTGCAG GGCTGAGGAC TATGGGGAGC ACCACCTGGC AGTGCTCACC AACTTGGGTG 
     3721 ACATCCAGGT GGTCTCAATG CCCCTGCTCA AGCCCCAGGT GCGGTACAGC TGCATCCGCC 
     3781 GGGAGGACGT CAGCGGCATA GCTTCCTGTG TCTTCACCAA ATATGGCCAA GGTTTCTATC 
     3841 TGATATCACC TTCGGAGTTC GAGCGCTTTT CTCTCTCCAC CAAGTGGCTG GTTGAACCCC 
     3901 GGTGTTTGGT GGATTCAACC AAAGCCAAGA AGCACAACCG GCCCAGCAAT GGCAATGGCA 
     3961 CAGGCCTCAA AATGACCTCC TCAGGCCACG TCAGGAACTC AAAGAGCCAA AGTGATGGAG 
     4021 ATGAGAAGAA GCCGGGCCCA GTGATGGAGC ACGCGCTGCT CAATGACGCC TGGGTCCTAA 
     4081 AGGAAATCCA GAGCACGCTG GAGGGGGACC GGAGGAGCTA TGGCAATTGG CATCCTCACC 
     4141 GCGTGGCTGT GGGTTGCAGG CTCAGCAATG GAGAAGCAGA AACGCGTACG CGGCCGCTCG 
     4201 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4261 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4321 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4381 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4441 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4501 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4561 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4621 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4681 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4741 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4801 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4861 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4921 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4981 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5041 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5101 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5161 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5221 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5281 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5341 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5401 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5461 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5521 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5581 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5641 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5701 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5761 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5821 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5881 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5941 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6001 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6061 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6121 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6181 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6241 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6301 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6361 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6421 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6481 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6541 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6601 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6661 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6721 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6781 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6841 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6901 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6961 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7021 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7081 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7141 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7201 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7261 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7381 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7441 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7501 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7561 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7621 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7681 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7741 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7801 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7861 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7921 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7981 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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