LOCUS       MR231509                8001 bp    DNA     circular  SYN 
DEFINITION  Atp4a (myc-DDK-tagged) - Mouse ATPase, H+/K+ exchanging, gastric, alpha polypeptide (Atp4a), transcript variant 1
ACCESSION   NM_001290627
VERSION     NM_001290627.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4156..4247 
                     /label="Myc-DDK"  
     misc_feature    4277..4862 
                     /label="PolyA signal"  
     misc_feature    4334..4353 
                     /label="Restriction Enzyme"  
     misc_feature    5138..5810 
                     /label="ColE1"  
     misc_feature    6240..7034 
                     /label="Kan/Neo"  
     misc_feature    7069..7371 
                     /label="SV40 ori"  
     misc_feature    7457..7934 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4131..4138 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4131 
                     /label="Atp4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GGGAAGGCAG AGAATTATGA ACTGTACTCA GTGGAACTGG 
     1081 GGTCTGGCCC TGGTGGGGAC ATGACTGCCA AGATGAGCAA GAAGAAGGCA GGAGGTGGGG 
     1141 GAGGCAAGAA GAAGGAGAAG CTGGAGAACA TGAAGAAGGA GATGGAGATT AACGACCACC 
     1201 AGCTATCAGT GTCTGAGCTG GAGCAGAAGT ATCAGACCAG CGCCACCAAG GGCCTGAAGG 
     1261 CGAGCCTGGC AGCTGAGCTG CTGCTGAGGG ATGGACCCAA TGCGCTCCGG CCACCTCGGG 
     1321 GCACTCCCGA GTATGTGAAG TTTGCCCGTC AGCTGGCAGG TGGTCTGCAG TGCCTCATGT 
     1381 GGGTGGCTGC TGCTATCTGC CTCATTGCCT TTGCCATCCA GGCCAGCGAG GGTGACCTGA 
     1441 CCACTGATGA TAATCTGTAC CTGGCGGTGG CACTCATTGC TGTGGTTGTG GTCACTGGCT 
     1501 GTTTTGGCTA CTACCAGGAG TTCAAGAGCA CAAATATCAT CGCCAGCTTC AAGAATCTTG 
     1561 TACCCCAGCA AGCCACTGTG ATCCGAGACG GGGACAAGTT CCAGATCAAC GCGGATCAGC 
     1621 TTGTGGTGGG CGACCTGGTG GAGATGAAAG GCGGGGACCG CGTGCCAGCA GACATCCGCA 
     1681 TTCTGTCAGC CCAGGGCTGC AAGGTGGACA ACTCCTCGCT CACTGGAGAG TCTGAACCGC 
     1741 AGACCCGCTC GCCCGAGTGT ACACATGAGA GTCCCCTTGA GACCCGCAAC ATCGCCTTCT 
     1801 TCTCCACCAT GTGTCTGGAG GGAACAGCTC AGGGTTTGGT GGTGAGCACC GGCGATCGCA 
     1861 CCATCATTGG GCGCATCGCC TCGCTGGCCT CGGGTGTGGA AAACGAGAAG ACTCCGATTG 
     1921 CAATCGAGAT TGAACATTTT GTGGACATCA TTGCCGGCCT GGCCATCCTC TTCGGTGCCA 
     1981 CATTCTTTGT GGTGGCCATG TGTATTGGCT ATACCTTCCT TCGGGCCATG GTCTTCTTCA 
     2041 TGGCCATTGT GGTAGCCTAT GTGCCTGAGG GGCTGCTGGC AACTGTCACA GTCTGCCTGT 
     2101 CACTGACAGC AAAGAGGTTG GCCAGTAAAA ACTGCGTGGT TAAAAACTTA GAAGCAGTTG 
     2161 AGACCCTAGG TTCTACATCA GTCATCTGCT CAGACAAGAC AGGAACTCTC ACTCAGAACC 
     2221 GCATGACGGT GTCTCATCTA TGGTTTGACA ACCATATCCA CACGGCGGAC ACCACAGAAG 
     2281 ACCAGTCAGG GCAAACGTTC GATCAGTCGT CGGAGACATG GCGAGCGCTG TGCCGCGTGC 
     2341 TCACCCTGTG CAACCGCGCT GCCTTCAAGT CCGGCCAGGA CGCGGTGCCG GTGCCCAAGC 
     2401 GCATCGTGAT CGGAGACGCG TCAGAGACTG CGCTGCTCAA GTTTTCGGAG CTGACGTTGG 
     2461 GCAACGCCAT GGGTTACAGG GACCGCTTCC CCAAAGTTTG CGAGATCCCC TTCAACTCCA 
     2521 TCAACAAGTT CCAGCTGTCC ATTCATACCC TGGAGGATCC ACGCGACTCC CGGCACCTGC 
     2581 TGGTGATGAA GGGCGCCCCT GAGCGCGTGC TGGAGCGTTG CAGCTCCATC CTCATCAAGG 
     2641 GCCAGGAGCT GCCTCTGGAC GAGCAATGGC GTGAGGCCTT CCAGACAGCC TACCTCAGCC 
     2701 TGGGAGGCCT AGGCGAACGT GTTCTTGGTT TCTGCCAGCT CTACCTGAAT GAGAAGGACT 
     2761 ACCCACCTGG CTATGCCTTT GATGTGGAGG CCATGAACTT TCCGAGTAGT GGCCTCTGCT 
     2821 TTGCGGGACT TGTATCCATG ATTGACCCTC CCCGGGCCAC CGTTCCTGAT GCTGTGCTCA 
     2881 AATGCCGCAC GGCAGGCATC CGGGTGATCA TGGTGACCGG TGACCATCCC ATCACGGCCA 
     2941 AGGCCATCGC AGCCAGCGTG GGTATCATCT CAGAGGGCAG CGAGACAGTG GAAGACATCG 
     3001 CCGCCCGCCT CCGGATGCCG GTGGACCAGG TTAATAGGAA GGATGCCCGG GCCTGCGTGA 
     3061 TCAATGGTAT GCAGCTGAAG GACATGGACC CGTCTGAGCT GGTGGAGGCG CTACGCACCC 
     3121 ACCCTGAGAT GGTGTTTGCT CGAACCAGTC CTCAGCAGAA GCTGGTGATT GTGGAGAGTT 
     3181 GTCAGCGACT GGGTGCCATT GTGGCTGTAA CAGGGGATGG TGTGAATGAC TCCCCAGCCC 
     3241 TGAAGAAAGC TGACATTGGT GTAGCCATGG GCATTGCTGG CTCGGATGCT GCTAAAAATG 
     3301 CTGCTGACAT GATCTTGCTG GATGACAACT TTGCTTCCAT TGTGACAGGC GTGGAGCAGG 
     3361 GCCGACTGAT CTTTGACAAC CTGAAGAAAT CCATTGCATA TACACTGACC AAGAACATTC 
     3421 CAGAACTGAC ACCCTACCTT ATCTATATCA CTGTCAGTGT GCCCCTACCC CTTGGGTGTA 
     3481 TCACCATTCT CTTCATAGAA CTCTGCACAG ATATCTTTCC ATCTGTGTCC CTGGCGTACG 
     3541 AGAAGGCTGA GAGCGACATC ATGCACCTGC GCCCACGGAA CCCTAAGCGT GACCGGTTGG 
     3601 TCAACGAACC CCTGGCTGCT TATTCCTATT TCCAGATTGG TGCCATTCAG TCATTTGCCG 
     3661 GCTTTGCTGA CTACTTCACG GCCATGGCGC AGGAGGGCTG GTTCCCTCTG TTGTGTGTAG 
     3721 GACTGCGGCC ACAATGGGAG GACCACCATC TACAAGATCT TCAAGACAGC TACGGCCAGG 
     3781 AATGGACATT TGGTCAGCGC CTGTACCAGC AGTACACCTG TTACACCGTG TTCTTCATCA 
     3841 GCATCGAGAT GTGCCAGATC GCAGATGTCC TCATCCGCAA GACACGGCGC CTCTCTGTTT 
     3901 TCCAGCAGGG ATTCTTCAGG AACAAGATCC TGGTGATCGC CATCGTGTTC CAGGTCTGCA 
     3961 TTGGCTGCTT CCTGTGCTAC TGCCCAGGGA TGCCTAACAT CTTCAACTTC ATGCCCATCC 
     4021 GGTTCCAGTG GTGGCTGGTC CCCATGCCCT TCGGCCTTCT CATCTTTGTC TATGATGAGA 
     4081 TCCGGAAACT TGGAGTTCGC TGTTGCCCAG GGAGCTGGTG GGACCAGGAA CTCTACTATA 
     4141 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4201 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4981 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5041 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5161 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5221 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5281 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5341 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5461 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5521 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5581 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5641 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5761 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5821 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5881 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5941 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6001 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6061 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6181 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6241 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6301 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6361 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6421 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6481 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6541 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6601 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6661 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6721 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6781 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6841 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6901 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6961 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7021 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7081 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7141 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7201 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7261 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7321 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7441 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7501 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7561 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7621 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7681 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7741 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7801 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7861 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7921 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7981 CAAAAATTTA ACGCGAATTT T
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