LOCUS       MR231508                7983 bp    DNA     circular  SYN 
DEFINITION  Ogdh (myc-DDK-tagged) - Mouse oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), transcript variant 2
ACCESSION   NM_001252283
VERSION     NM_001252283.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4140..4231 
                     /label="Myc-DDK"  
     misc_feature    4261..4846 
                     /label="PolyA signal"  
     misc_feature    4318..4337 
                     /label="Restriction Enzyme"  
     misc_feature    5122..5794 
                     /label="ColE1"  
     misc_feature    6224..7018 
                     /label="Kan/Neo"  
     misc_feature    7053..7355 
                     /label="SV40 ori"  
     misc_feature    7441..7918 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4122..4128 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4122 
                     /label="Ogdh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCATTTA AGGACTTGTG CTGCTAAGTT AAGGCCATTG ACAGCCTCCC 
     1081 AGACTGTTAA GACATTTTCA CAAAACAAAC CAGCAGCAAT TAGGACGTTT CAACAGATTC 
     1141 GGTGCTATTC TGCACCTGTA GCTGCTGAAC CATTTCTTAG TGGGACTAGT TCGAACTATG 
     1201 TGGAGGAAAT GTACTGTGCC TGGTTGGAGA ATCCCAAAAG TGTACATAAG TCATGGGACA 
     1261 TTTTTTTCCG AAACACCAAT GCTGGAGCCC CACCGGGCAC TGCCTACCAG AGCCCCCTTT 
     1321 CCCTGAGTCG AAGCTCCCTG GCTACCATGG CCCATGCACA GTCCCTGGTG GAAGCACAAC 
     1381 CTAACGTCGA CAAACTCGTG GAGGACCACT TGGCGGTGCA GTCTCTCATC AGGGCATATC 
     1441 AGGTCAGGGG TCACCACATT GCAAAACTTG ATCCTCTCGG AATTAGTTGT GTAAATTTTG 
     1501 ATGATGCTCC GGTAACTGTT TCTTCAAACG TGGATCTTGC AGTTTTCAAG GAACGACTTC 
     1561 GAATGCTAAC AGTAGGAGGG TTCTATGGCC TACACGAGTC TGACCTTGAC AAGGTCTTCC 
     1621 ACTTACCCAC CACCACTTTC ATCGGGGGAC AGGAGCCAGC ACTTCCTCTT CGGGAGATCA 
     1681 TCCGTCGGCT GGAGATGGCC TACTGCCAGC ACATTGGTGT GGAGTTCATG TTCATTAATG 
     1741 ATTTGGAACA ATGCCAGTGG ATCCGACAGA AGTTTGAGAC CCCTGGAATC ATGCAGTTCA 
     1801 CCAATGAGGA GAAGCGGACC TTGCTGGCCA GGCTTGTACG ATCCACCAGG TTTGAGGAGT 
     1861 TCCTACAGCG AAAGTGGTCC TCGGAGAAGC GTTTTGGTCT GGAAGGCTGT GAGGTGCTGA 
     1921 TCCCTGCCCT CAAGACAATC ATTGATATGT CAAGTGCAAA TGGAGTGGAC TATGTGATCA 
     1981 TGGGGATGCC ACACAGAGGA CGGCTGAACG TGCTTGCAAA TGTCATCAGG AAGGAGCTGG 
     2041 AGCAAATATT CTGTCAGTTT GACTCAAAGC TGGAGGCAGC TGATGAGGGT TCTGGGGACA 
     2101 TGAAGTACCA CCTGGGCATG TATCACCGCA GGATCAACCG TGTGACCGAC AGAAACATCA 
     2161 CTTTGTCCTT GGTGGCTAAC CCTTCCCATC TAGAGGCTGC TGACCCTGTC GTGATGGGAA 
     2221 AGACCAAAGC TGAACAGTTC TACTGTGGAG ACACTGAAGG GAAAAAGGTG ATGTCTATCC 
     2281 TGCTGCATGG GGATGCTGCT TTTGCTGGCC AGGGCATCGT ATATGAGACC TTCCATCTCA 
     2341 GCGACTTGCC GTCCTACACA ACCCATGGCA CTGTTCATGT GGTTGTCAAC AACCAGATTG 
     2401 GCTTCACCAC AGACCCTCGG ATGGCCCGCT CCTCTCCCTA CCCCACTGAT GTGGCCCGAG 
     2461 TGGTGAATGC CCCCATTTTC CATGTCAACT CAGATGACCC TGAAGCTGTC ATGTATGTAT 
     2521 GCAAGGTGGC AGCTGAGTGG AGAAACACCT TCCACAAGGA TGTTGTAGTT GATCTGGTGT 
     2581 GTTATCGACG AAATGGCCAC AATGAGATGG ACGAACCTAT GTTTACACAG CCACTCATGT 
     2641 ACAAGCAGAT CCGCAAGCAG AAGCCTGTAC TGCAGAAGTA TGCAGAATTG CTAGTCTCCC 
     2701 AGGGTGTCGT CAATCAGCCT GAGTACGAGG AGGAAATCTC CAAGTATGAT AAGATCTGTG 
     2761 AGGAAGCATT TACCAGATCC AAAGATGAGA AGATCTTGCA CATCAAGCAC TGGCTGGATT 
     2821 CCCCCTGGCC TGGCTTTTTC ACCCTGGATG GACAGCCCAG GAGCATGACC TGCCCCTCCA 
     2881 CTGGCCTGGA GGAGGATGTC TTGTTCCACA TTGGAAAGGT GGCCAGCTCT GTACCTGTGG 
     2941 AGAACTTTAC TATCCATGGA GGGCTGAGCC GGATCTTGAA GACCCGCAGA GAGCTTGTGA 
     3001 CGAACCGGAC TGTGGACTGG GCCCTGGCAG AGTACATGGC ATTTGGCTCA CTGCTGAAGG 
     3061 AAGGCATCCA TGTGCGGCTG AGTGGCCAGG ATGTGGAGCG GGGCACCTTC AGCCATCGCC 
     3121 ACCATGTGCT CCATGATCAG AATGTTGACA AAAGAACCTG CATCCCCATG AACCACCTTT 
     3181 GGCCAAATCA GGCCCCTTAC ACTGTATGCA ACAGCTCGCT GTCTGAGTAC GGTGTCCTGG 
     3241 GCTTTGAGCT GGGCTTTGCC ATGGCTAGCC CTAATGCTCT GGTTCTCTGG GAGGCCCAGT 
     3301 TTGGTGACTT CAACAACATG GCACAGTGCA TCATTGACCA GTTCATCTGC CCAGGACAGG 
     3361 CAAAGTGGGT GCGGCAGAAT GGCATTGTGC TCCTGCTGCC TCATGGCATG GAAGGCATGG 
     3421 GTCCCGAGCA TTCCTCTGCC CGCCCAGAGC GGTTTCTGCA GATGTGCAAT GATGACCCAG 
     3481 ATGTCCTGCC TGACCTGCAG GAAGAAAACT TTGACATCAA TCAGCTCTAT GACTGCAACT 
     3541 GGATTGTTGT CAACTGTTCC ACCCCTGGCA ACTTCTTCCA TGTGCTGCGA CGACAGATCT 
     3601 TGCTGCCCTT CCGGAAGCCG TTAATCGTCT TCACTCCCAA ATCCCTCCTG CGCCACCCTG 
     3661 AGGCAAGAAC TAGCTTTGAC GAGATGCTGC CAGGAACGCA CTTCCAGCGT GTGATCCCAG 
     3721 AAAATGGACC TGCAGCTCAG GACCCACACA AAGTCAAGAG ACTTCTCTTC TGCACTGGGA 
     3781 AGGTGTACTA TGACCTCACC CGAGAGCGCA AAGCCAGGAA CATGGAGGAG GAGGTGGCTA 
     3841 TTACAAGGAT TGAGCAGCTA TCACCATTCC CCTTTGACCT CCTGCTGAAA GAGGCACAGA 
     3901 AGTATCCCAA TGCTGAGCTG GCCTGGTGCC AGGAAGAGCA CAAGAACCAA GGCTACTATG 
     3961 ACTATGTCAA GCCAAGACTT CGTACCACCA TTGACCGTGC TAAGCCTGTC TGGTATGCTG 
     4021 GCCGAGACCC GGCAGCTGCT CCAGCCACTG GCAACAAGAA AACACACCTG ACAGAGCTGC 
     4081 AGCGCTTTCT GGACACAGCC TTTGACCTGG ACGCATTCAA GAAATTCTCT ACGCGTACGC 
     4141 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4201 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4261 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4321 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4381 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4441 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4501 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4561 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4621 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4681 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4741 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4801 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4861 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4921 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4981 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5041 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5101 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5161 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5221 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5281 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5341 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5401 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5461 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5521 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5581 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5641 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5701 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5761 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5821 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5881 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5941 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6001 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6061 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6121 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6181 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6241 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6301 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6361 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6421 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6481 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6541 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6601 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6661 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6721 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6781 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6841 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6901 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6961 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7021 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7081 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7141 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7201 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7261 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7381 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7441 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7501 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7561 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7621 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7681 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7741 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7801 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7861 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7921 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7981 TTT
//