LOCUS       MR231503                7977 bp    DNA     circular  SYN 
DEFINITION  Dgkh (myc-DDK-tagged) - Mouse diacylglycerol kinase, eta (Dgkh), transcript variant 3
ACCESSION   NM_001281794
VERSION     NM_001281794.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4134..4225 
                     /label="Myc-DDK"  
     misc_feature    4255..4840 
                     /label="PolyA signal"  
     misc_feature    4312..4331 
                     /label="Restriction Enzyme"  
     misc_feature    5116..5788 
                     /label="ColE1"  
     misc_feature    6218..7012 
                     /label="Kan/Neo"  
     misc_feature    7047..7349 
                     /label="SV40 ori"  
     misc_feature    7435..7912 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4116..4122 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4116 
                     /label="Dgkh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTATGTGCT GAGAACAGGA AAGAGATGGA GGTCTGGATC AGCTCTCTGA 
     1081 AGTCAGTGCA GAGCAGAGAA CCCTATGAGG TGGCCCAGTT CAATGTGGAA CATTTCTCAG 
     1141 GGATGCACAA CTGGTACGCC TGCTCCCACG CCCGGCCCAC CTTCTGTAAC GTGTGCAGAG 
     1201 AGAGTCTCTC TGGAGTGACC TCCCACGGCC TGTCCTGTGA AGTGTGTAAG TTCAAGGCTC 
     1261 ATAAAAGATG TGCAGTGAGG GCAACAAACA ACTGCAAATG GACCACGCTG GCCTCCATCG 
     1321 GGAAGGACAT CATAGAGGAC GAGGATGGTG TTGCCATGCC TCACCAGTGG CTCGAAGGCA 
     1381 ACTTGCCTGT GAGTGCCAAG TGTGCAGTCT GTGACAAAAC CTGTGGCAGT GTGCTTCGCC 
     1441 TGCAAGATTG GAAATGCCTT TGGTGTAAGA CAATGGTGCA CACTGCCTGC AAAGATGTGT 
     1501 ACCATCCCGT CTGTCCCCTT GGCCAGTGTA AAGTCTCCAT CATACCTCCG ATTGCACTAA 
     1561 ATAGCACAGA CTCTGATGGT TTCTGTCGAG CAACCTTTTC GTTCTGTGTC AGCCCCCTCT 
     1621 TGGTGTTCGT CAACTCTAAG AGTGGAGATA ATCAGGGAGT GAAGTTCCTT CGTCGCTTTA 
     1681 AACAGTTGCT CAACCCAGCT CAGGTGTTTG ATTTAATGAA CGGAGGGCCT TATTTAGGCT 
     1741 TGAGATTGTT TCAAAAGTTT GACAACTTTC GGATCCTTGT CTGTGGAGGA GATGGAAGTG 
     1801 TAGGCTGGGT TTTGTCAGAG ATTGATAAAC TGAACTTGCA TAAACAGTGC CAGCTTGGGG 
     1861 TGCTACCCTT GGGTACCGGC AATGACCTTG CTCGAGTTCT TGGCTGGGGA GGTTCATATG 
     1921 ATGATGACAC CCAACTCCCT CAGATACTAG AGAAGCTGGA ACGAGCCAGT ACCAAAATGC 
     1981 TGGACAGGTG GAGTATAATG ACCTATGAGC TCAAATTGCC AGCAAAGTCG TCTCTACTTC 
     2041 CTGAACCTGT GGCAGCAACT GAAGAATTTT ACATGACAAT TTATGAAGAC TCCGTTGCAA 
     2101 ACCATCTTAC GAAAATCGTC AACTCTGATG AGCATGCGGT GGTCATATCG TCTGCCAAGA 
     2161 TACTCTGTGA AACCGTGAAG GACTTCGTTG CCAAAGTGGA GAAGGCACAG GACAGAACAC 
     2221 TGGAAAATAC AGTAGTAGCC GAAGCCGTGG CCAGTAAATG CTCAGTCCTA AACGAGAAGC 
     2281 TTGAGCAGTT GCTACAAGCC TTGCACGCGG ACTCTCAGGC CAGTCGAGTC CCCCCAGGAA 
     2341 TCGGCCCTGC CATCCCCGAA GAGGACACCG TGGAGTCCGC CAGTGACGAG TCCTTGGGTG 
     2401 AAAGCAAGGA CCAGCTGGTG AATGACATTG GGAAACCCTC CTCCCAGAAA GCCGTGAAGC 
     2461 CCCGGGAGAT AATGCTGCGG GCCAACAGTC TAAAGAAAGC GGTGAGGCAA GTCATTGAAG 
     2521 AAGCTGAAAA AGTTATGGAT GAGCCAGCAG TGCAGCCCTC TGAGCCAGTC AGTCCGTCTT 
     2581 GTGACTATGA TACAGAAACA GATGAAGCTA AAGAGGACGA CGCGAAAGAG TCGCTATCAG 
     2641 CCAAAACCAC ATCTCAGTCT CCTGATGCCC AGGCAAGCTG TGGCCATCCC CAAACTGACT 
     2701 CTGTCGCTGG TCCAGCCATG GCCACCACCA AAGAAAACCT CCCTGTGCTC AACACTAGAA 
     2761 TCATCTGCCC AGGTTTGAGA GCAGGACTTG CTGCCTCCAT TGCTGGGAGT TCTATCATCA 
     2821 ACAAAATGTT GCTGGCGAAC ATCGATCCTT TCGGGGCAAC ACCGTTTATT GACCCAGATC 
     2881 TAGACTCACT AGATGGATAC TCTGAAAAAT GTGTCATGAA CAACTACTTT GGGATTGGAT 
     2941 TAGATGCAAA AATTTCATTA GAATTTAATA ATAAGAGAGA GGAGCACCCT GAAAAATGCA 
     3001 GAAGTCGAAC TAAAAACTTG ATGTGGTACG GAGTCCTTGG GACCCGGGAG CTACTACAGA 
     3061 GATCGTACAA GAATCTGGAG CAGAGGGTTC AACTTGAGTG TGATGGGCAG TATATACCTC 
     3121 TTCCTAGTCT ACAAGGCATA GCGGTGTTGA ACATCCCTAG CTACGCCGGA GGCACCAACT 
     3181 TCTGGGGCGG AACCAAAGAG GATGATATAT TTGCTGCGCC ATCCTTTGAT GACAAGATTC 
     3241 TAGAAGTTGT CGCAGTGTTT GACAGTGTGC AGATGGCAGT ATCAAGGGTC ATTAAATTGC 
     3301 AGCATCACCG GATAGCACAG TGCCGTACGG TAAAAATCAC GATATTTGGC GACGAGGGAG 
     3361 TCCCAGTGCA GGTGGATGGT GAAGCATGGG TCCAGCCTCC AGGGATCATC AAGATTGTGC 
     3421 ACAAAAACAG AGCTCAGATG CTAACCAGAG ACAGAGCCTT TGAAAGCACC CTGAAGTCTT 
     3481 GGGAAGACAA GCAGAAGTGT GACTCTGGGA AGCCAGTTCT TCGAACCAAC CTGTACATCC 
     3541 ATCCCGCCCC TGACCTGGCA ACAGAGGAAG TGTCACAGAT GCGGCTCTGC TCCCAGGCTG 
     3601 CGGAGGAACT CATCACTAGG ATTTGTGACG CAGCCACGAT TCACTGTCTC CTGGAGCAGG 
     3661 AGCTGGCACA TGCAGTGAAT GCCTGCTCAC ACGCCCTGAA CAAAGCCAAC CCACGCTTCC 
     3721 CGGAGAGCCT TACGAGAGAC ACTGCCACCG AGATAGCCAT CAACGTCAAG GCGCTCTATA 
     3781 ATGAGACAGA GGCTTTGCTA GTTGGCAGGG TCCCTTTGCA CTTGGAATCT CCTCACGAAG 
     3841 AGCGGGTGTC CAGTGCCTTA CACTCTGTGG AGATGGAGCT TCAGAAGTTA ACGGAGATTC 
     3901 CCTGGCTTTA TTACATCCTC CGCCCCAGTG AGGACGAGGA GCCTCCTCTG GATTGTACCA 
     3961 AAAGAAACAA CAAAAGCACA GTCTTTCGCA TAGTGCCAAA GTTTAAAAAG GAAAAGGCTC 
     4021 AGAAACAGAA AACAAGTTCG CAGCCTGAAT TGGGGCACAG AGGAAGTTGC TGCTTGGCTG 
     4081 GATCTGCTCA ATTTGGGAGA GTACAAAGAA ATCTTCATCC GTCAACGCGT ACGCGGCCGC 
     4141 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4201 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4261 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4321 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4381 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4441 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4501 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4561 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4621 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4681 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4741 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4801 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4861 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4921 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4981 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5041 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5101 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5161 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5221 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5281 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5341 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5401 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5461 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5521 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5581 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5641 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5701 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5761 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5821 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5881 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5941 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6001 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6061 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6121 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6181 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6241 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6301 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6361 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6421 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6481 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6541 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6601 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6661 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6721 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6781 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6841 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6901 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6961 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7021 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7081 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7141 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7201 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7381 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7441 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7501 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7561 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7621 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7681 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7741 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7801 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7861 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7921 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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