LOCUS       MR231480                7914 bp    DNA     circular  SYN 
DEFINITION  Lig3 (myc-DDK-tagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), transcript variant 3
ACCESSION   NM_001291246
VERSION     NM_001291246.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4071..4162 
                     /label="Myc-DDK"  
     misc_feature    4192..4777 
                     /label="PolyA signal"  
     misc_feature    4249..4268 
                     /label="Restriction Enzyme"  
     misc_feature    5053..5725 
                     /label="ColE1"  
     misc_feature    6155..6949 
                     /label="Kan/Neo"  
     misc_feature    6984..7286 
                     /label="SV40 ori"  
     misc_feature    7372..7849 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4053..4059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4053 
                     /label="Lig3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTTTGGCT TTCAAGATCC TCTTCCCGAG AAACCTTTGT GCCCTTGGCA 
     1081 GAAAAGAACT GTGCCTGTTC CCAGAACAGA ATCGCTGGGC TGCCATAAGT CAGTTCAGCC 
     1141 AGTGGTCGGA AACAAATCTC CTCGGTGGGT GCTGCCTTCT CCAGAGAAGA AAGCCTGTCC 
     1201 TAGCTCTCCA GAGAGGTCAT CTAAGACCAC GTGCCACCCA CCTTACTTTC TGGCCAGGGT 
     1261 CGCATGTGGG ACTCTGTACT GGCCCCTGTG CGATGGCAGA GCAGCGGTTC TGTGTGGACT 
     1321 ATGCCAAGCG GGGCACAGCT GGATGCAAGA AATGCAAGGA GAAGATTGTA AAGGGCGTGT 
     1381 GCCGCATTGG CAAAGTGGTG CCCAACCCCT TCTCAGAGTC TGGGGGTGAT ATGAAAGAGT 
     1441 GGTACCACAT TAAGTGCATG TTTGAGAAAC TGGAACGGGC TCGGGCCACC ACAAAGAAAA 
     1501 TTGAAGACCT CACAGAGCTG GAAGGCTGGG AAGAGCTGGA AGATAACGAA AAGGAACAGA 
     1561 TAAGCCAGCA TATTGCAGAC CTGTCTTCTA AGGCAGCTGC CACACCTAAG AAGAAAGCTG 
     1621 CTGTCCAGGC TAAGCTGACA ACCACTGGCC AGGTGACATC TCCAGTGAAA GGTGCTTCGT 
     1681 TTATTACCAG TACCAATCCT CGGAAGTTCT CTGGCTTTTC AGCAAAACCC AACAACTCTG 
     1741 AGCAAGCCCC CTCAAGCCCT GCTCCTGGAA CGAGTCTGTC TGCAAGTAAA TGTGACCCTA 
     1801 AGCACAAAGA TTGTCTGCTA CGAGAGTTCC GGAAGCTGTG CGCCATGGTG GCTGAAAATC 
     1861 CTAGCTATAA CACCAAGACC CAGATCATCC ATGACTTCTT GCAGAAAGGC TCCACAGGAG 
     1921 ATGGCTTTCA CGGTGATGTG TACCTGACAG TTAAGCTGCT GCTGCCTGGT GTCATCAAGA 
     1981 GTGTTTACAA CTTGAATGAT AAGCAGATTG TAAAACTTTT TAGCCGAATT TTTAACTGCA 
     2041 ACCCAGATGA TATGGCCCGG GACCTAGAAC AGGGTGACGT GTCAGAGACG ATCAGAATCT 
     2101 TCTTTGAGCA GAGCAAGTCT TTCCCCCCAG CTGCCAAAAG CCTCCTCACC ATTCAGGAAG 
     2161 TGGATGCCTT CCTCCTGCAC CTCTCCAAGC TCACCAAAGA GGATGAGCAG CAGCAGGCCC 
     2221 TGCAGGACAT AGCCTCCCGG TGTACAGCCA ATGACCTTAA GTGCATTATT CGGCTGATCA 
     2281 AACATGATCT GAAGATGAAC TCGGGTGCAA AACACGTGTT AGATGCCCTT GACCCCAATG 
     2341 CTTATGAAGC CTTCAAAGCA TCGCGCAACC TGCAGGATGT GGTGGAGCGA GTCCTTCACA 
     2401 ATGAGCAGGA GGTGGAGAAG GACCCAGGCC GGCGACGGGC TCTGAGGGTC CAGGCCTCAC 
     2461 TGATGACGCC TGTGCAGCCC ATGCTGGCTG AGGCCTGCAA ATCCATTGAA TATGCAATGA 
     2521 AGAAGTGTCC CAATGGCATG TTCTCTGAGA TCAAGTACGA TGGTGAGCGA GTCCAGGTGC 
     2581 ATAAGAAGGG GGACCACTTC AGCTACTTCA GCCGCAGTCT CAAGCCTGTC CTGCCTCACA 
     2641 AGGTGGCCCA CTTCAAGGAC TATATACCCA AAGCTTTTCC TGGGGGTCAA AGCATGATCT 
     2701 TGGATTCCGA GGTACTCCTG ATTGACAACA ACACAGGCAA ACCACTGCCC TTTGGGACTC 
     2761 TGGGAGTGCA CAAGAAAGCT GCTTTCCAGG ATGCTAATGT CTGCCTGTTT GTTTTTGATT 
     2821 GTATCTACTT TAATGATGTC AGCTTGATGG ACAGGCCTCT CTGTGAGCGA CGGAAGTTTC 
     2881 TTCATGACAA CATGGTTGAA ATCCGTAACC GGATCATGTT CTCAGAAATG AAGCAAGTCA 
     2941 CAAAAGCTTC AGACCTGGCT GACATGATAA ACCGGGTGAT CCGAGAGGGC TTGGAAGGGT 
     3001 TGGTGCTAAA GGATGTGAAG GGTACATATG AGCCTGGGAA GCGGCACTGG CTGAAAGTTA 
     3061 AGAAAGATTA TTTGAATGAG GGGGCCATGG CTGATACAGC TGATCTGGTG GTTCTTGGGG 
     3121 CCTTCTATGG GCAAGGGAGC AAAGGCGGAA TGATGTCCAT CTTCCTCATG GGCTGCTATG 
     3181 ACCCTGACAG CCAAAAGTGG TGCACTGTTA CAAAATGTGC TGGAGGCCAT GATGATGCCA 
     3241 CACTTGCCCG CCTGCAGAAA GAGCTGGTTA TGGTGAAGAT CAGCAAGGAT CCTAGTAAGA 
     3301 TACCCAGCTG GCTGAAGATC AACAAGATCT ATTATCCTGA CTTTATCGTC CCAGACCCAA 
     3361 AGAAAGCTGC TGTGTGGGAG ATCACAGGGG CAGAATTCTC CAGATCTGAG GCACACACTG 
     3421 CTGATGGCAT CTCCATCCGA TTTCCTCGAT GCACCCGAAT CCGGGACGAC AAGGACTGGA 
     3481 AATCTGCCAC TAACCTCCCC CAACTCAAGG AACTGTACCA GCTGTCCAAA GACAAGGCAG 
     3541 ACTTTGCTGT GGTGGCTGGA GATGAGGCAA GCCCCACTAC TGGAGGCAGC AGTGGCGAGA 
     3601 ACGAGGGCAC TGCTGGGTCT GCTGGGCCCT GCAAGGGCCC CCCCAGTAAG TCCTCTGCCA 
     3661 GTGCCAAGAC CACCGAACAG AAGCTCAATA GCCCCAGTAG CAGAGGTGGC ATCAAACCGA 
     3721 TTCCAAAGCA TTCCCCCATG AAACCAGGAG AGAAGCTGGC TGTGAAGTCT TCTCCAGTCA 
     3781 AAGTGGGAAT GAAGAGGAAA GCTACTGATG AGACCCCATG CCTGAAAAAG GTACTGTTGG 
     3841 ATGTCTTCAC TGGGGTGCGG CTCTACTTGC CACCTTCTAC ACCAGACTTC AAACGTCTCA 
     3901 AACGCTACTT TGTGGCATTC GACGGGGACC TGGTACAGGA ATTTGACATG GGCTCAGCCA 
     3961 CACATGTGCT AGGTAACAGG GAAAAGAACA CTGATGCCCA GTTGGTCTCC TCAGAGTGGA 
     4021 TTTGGGCATG TATCCGGAAA CGGAGGCTGA TAGCTCCCTG CACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//