LOCUS       MR231417                7734 bp    DNA     circular  SYN 
DEFINITION  Pkn1 (myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 1
ACCESSION   NM_001199593
VERSION     NM_001199593.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3891..3982 
                     /label="Myc-DDK"  
     misc_feature    4012..4597 
                     /label="PolyA signal"  
     misc_feature    4069..4088 
                     /label="Restriction Enzyme"  
     misc_feature    4873..5545 
                     /label="ColE1"  
     misc_feature    5975..6769 
                     /label="Kan/Neo"  
     misc_feature    6804..7106 
                     /label="SV40 ori"  
     misc_feature    7192..7669 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3873..3879 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3873 
                     /label="Pkn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCTGAT CCCCCTTTGG ATTCGGAGCT GGAGAGTGAG CCTCGAAGCT 
     1081 GGTCGCTGCT GGAACAGCTG GGTCTGGCTG GGGCTGACCT GGCAGCCCCC GGGGTGCAGC 
     1141 AACAGCTGGA GTTAGAGCGA GAGCGACTGA AGCGGGAGAT ACGGAAGGAG CTGAAGCTGA 
     1201 AGGAGGGCGC TGAGAACCTG AGGCGAGCCA CCACTGACCT GGGCCGTAGC CTGGCCCCTG 
     1261 TGGAGCTGCT GCTGCGGGGC TCCGCTCGAC GCCTAGACTT ACTGCACCAG CAGCTGCAGG 
     1321 AGCTGCATGC ACATGTGGTG CTGCCTGACC CTGCAGCTGG AAGCGATGCT ACCCAATCCC 
     1381 TTGCAGAGGG CAGCCCTATC TGCTCATCCA CCAACCTGAG CAGAGTGGCT GGGCTGGAGA 
     1441 AGCAGCTGGC CATTGAGCTC AAGGTCAAAC AGGGGGCAGA GAACATGATC CAGACCTATA 
     1501 GCAATGGCAG CAGCAAGGAC CGGAAGCTGT TGTTGACAGC CCAGCAGATG CTGCAGGACA 
     1561 GTAAGACCAA GATTGACATC ATCCGCATGC AGCTTCGAAG AGCGCTCCAG GCACTACAGG 
     1621 CTGGAGAGCT GGAGAGTCAG GCAGCTCCTG ATGAAGCCCA AGGAGATCCA GAACTGGGAG 
     1681 CCGTAGAGCT ACGCATTGAG GAGCTACGAC ACCATTTTCG AGTGGAGCAT GCAGTGGCAG 
     1741 AAGGTGCCAA GAATGTCCTG CGCCTGCTCA GTGGGGCAAA GGCCCCAGAC CGCAAGGCAG 
     1801 TCAGCGAGGC TCAGGAGAAA TTGACTGAGT CCAACCAGAA GCTGGGCTTG CTGCGGGAAT 
     1861 CACTGGAGAG GCGCCTTGGG GAGTTGCCTG CCGATCACCC CAAGGGGCGG CTGCTTCGGG 
     1921 AGGAGCTCAC TGCAGCCTCG TCCTCAGCCT TCAGCGCCAT ACTGCCTGGG CCCTTCCCTG 
     1981 CCACTCACTA CAGCACCTTG AGCAAGCCTG CACCGCTCAC AGGGACCCTG GAAGTACGAG 
     2041 TGGTGGGCTG CAAAAACCTT CCCGAGACCA TCCCCTGGAG CCCTCCCCCC TCAGTGGGGG 
     2101 CATCTGGGAC CCCCGAAAGC CGCACTCCGT TCCTGAGTCG CCCCGCTCGG GGCCTTTACA 
     2161 GCCGGAGTGG AAGCCTTAGT GGACGGAGCA GCCTCAGAGG GGAGGCAGAG AATGCCACTG 
     2221 AGGTCAGCAC CGTGCTCAAG CTGGACAACA CAGTGGTGGG GCAAACAGCC TGGAAGCCAT 
     2281 GCGGCCCCAA TGCCTGGGAC CAGAGCTTTA CCCTGGAGCT GGAGAGGGCT CGGGAGCTGG 
     2341 AGCTGGCTGT GTTCTGGCGA GACCAGAGGG GTCTGTGTGC TCTCAAATTT CTGAAGTTGG 
     2401 AGGACTTCTT GGACAACGAG AGGCATGAGG TGCAGCTGGA CATGGAACCC CAGGGCTGCC 
     2461 TGGTGGCTGA GGTCACTTTC CGAAACCCCA TCATCGAGCG GATCCCTAGG CTCCAAAGGC 
     2521 AGAAAAAAAT CTTCTCCAAG CAGCAAGGGA AGGCATTTCA GCGAGCCAGA CAGATGAACA 
     2581 TAGATGTGGC GACTTGGGTG CGGCTGCTCC GTAGACTCAT CCCTAGTGCT GTGGCCACTG 
     2641 GCACCTTCAG TCCCAATGCA TCTCCAGGTG CTGAGATCCG GCACACTGGA GACATATCCA 
     2701 TGGAGAAATT GAATCTCGGT GCTGACTCAG ACAGCTCGTC CCAAAAGAGC CCACCAGGGC 
     2761 TGCCCTCCAC CTCATGCAGC CTGAGTTCTC CAACCCATGA ATCCACCACA TCTCCAGAGC 
     2821 TGCCTTCAGA GACCCAGGAG ACTCCAGGCC CTGGCCTGTG CAGCCCCTTG AGAAAGTCGC 
     2881 CCCTGACACT TGAGGACTTC AAGTTCCTGG CCGTGCTTGG CCGGGGTCAC TTTGGAAAGG 
     2941 TGCTGCTGTC TGAATTCCGC TCCAGTGGGG AGCTCTTTGC CATCAAAGCC TTGAAGAAAG 
     3001 GTGACATTGT AGCCCGAGAT GAGGTTGAGA GCCTGATGTG TGAGAAGCGG ATTTTGGCGG 
     3061 CCGTGACCAG GGCAGGACAT CCCTTCCTGG TGAACCTTTT CGGCTGTTTC CAGACCCCAG 
     3121 AGCACGTGTG CTTTGTGATG GAGTACTCGG CGGGTGGAGA CCTGATGCTG CACATTCATA 
     3181 GCGACGTGTT CTCAGAGCCT CGGGCTGTCT TCTATTCGGC CTGTGTGGTG CTGGGACTGC 
     3241 AGTTCCTCCA TGAACACAAG ATTGTCTACA GGGACCTGAA GTTGGACAAT TTGCTCCTGG 
     3301 ATACTGAGGG CTACGTCAAG ATCGCAGACT TTGGCCTCTG CAAGGAGGGG ATGGGCTATG 
     3361 GGGACCGGAC CAGCACGTTC TGCGGAACTC CGGAGTTCCT GGCGCCGGAA GTGCTCACAG 
     3421 ACACATCCTA CACGCGAGCA GTGGACTGGT GGGGACTGGG CGTGCTGCTC TATGAGATGT 
     3481 TGGTTGGAGA GTCTCCGTTC CCTGGGGATG ATGAGGAGGA GGTATTTGAC AGCATTGTCA 
     3541 ACGACGAAGT TCGCTATCCC CGCTTCCTGT CTGCAGAGGC CATCGGCATC ATGAGAAGGC 
     3601 TACTGCGGAG GAACCCGGAG CGGAGGCTGG GGTCCACTGA GCGCGATGCA GAAGATGTGA 
     3661 AAAAACAGCC TTTCTTCCGG TCTCTGGGCT GGGATGTCCT GCTGGCCCGC CGCTTGCCTC 
     3721 CACCCTTCGT GCCTACACTT TCAGGGCGCA CAGATGTCAG CAACTTCGAT GAGGAGTTCA 
     3781 CTGGGGAGGC CCCCACACTG AGTCCTCCCC GGGATGCACG GCCCCTCACA GCTGCGGAGC 
     3841 AGGCAGCCTT CCGGGATTTC GACTTTGTGG CCGGAGGCTA CACGCGTACG CGGCCGCTCG 
     3901 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3961 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4021 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4081 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4141 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4201 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4261 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4321 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4381 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4441 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4501 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4561 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4621 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4681 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4741 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4801 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4861 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4921 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4981 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5041 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5101 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5161 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5221 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5281 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5341 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5401 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5461 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5521 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5581 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5641 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5701 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5761 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5821 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5881 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5941 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6001 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6061 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6121 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6181 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6241 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6301 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6361 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6421 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6481 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6541 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6601 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6661 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6721 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6781 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6841 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6901 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6961 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7081 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7141 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7201 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7261 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7321 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7381 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7441 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7501 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7561 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7621 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7681 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//