LOCUS       MR231414                7719 bp    DNA     circular  SYN 
DEFINITION  Pcsk6 (myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6), transcript variant 2
ACCESSION   NM_001291184
VERSION     NM_001291184.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3876..3967 
                     /label="Myc-DDK"  
     misc_feature    3997..4582 
                     /label="PolyA signal"  
     misc_feature    4054..4073 
                     /label="Restriction Enzyme"  
     misc_feature    4858..5530 
                     /label="ColE1"  
     misc_feature    5960..6754 
                     /label="Kan/Neo"  
     misc_feature    6789..7091 
                     /label="SV40 ori"  
     misc_feature    7177..7654 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3858..3864 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3858 
                     /label="Pcsk6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCAG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGATGTCGC GCGGGGCGCA GGGGCCGCCG GCCGGCACGG ACTCCCGCCG CTCGCGCTGC 
     1141 GCCCCTGGCG TTGGCTGCTT CTGCTCGCGC TGCCCGCCGC CTGCTCCGCG CTGCCGCCGC 
     1201 CGCGCCCCGT CTACACCAAC CACTGGGCAG TGCAAGTGCT GGGCGGCCCC GGCGCGGCGG 
     1261 ACCGCGTGGC TGCGGCGCAC GGCTACCTCA ACTTGGGCCA GATTGGAAAC CTGGACGATT 
     1321 ACTATCATTT TTACCACAGC AAGACCTTCA AGAGATCGAC CTTGAGCAGC AGGGGCCCCC 
     1381 ACACCTTCCT CAGAATGGAC CCACAGGTAA AATGGCTGCA ACAACAGGAA GTGAAGCGCA 
     1441 GAGTCAAGAG ACAGGCGCGA AGTGACTCTC TTTATTTCAA TGATCCCATT TGGTCTAACA 
     1501 TGTGGTACAT GCATTGTACT GATAAGAACA GTCGCTGTCG GTCAGAGATG AACGTCCAGG 
     1561 CGGCGTGGAA GCGTGGCTAC ACAGGAAAGA ATGTGGTCGT CACCATCCTC GATGACGGCA 
     1621 TAGAAAGGAA TCACCCAGAC CTGGCCCCCA ACTACGATTC CTATGCAAGC TACGATGTCA 
     1681 ATGGAAATGA TTATGACCCA TCCCCGAGAT ATGACGCCAG CAACGAGAAC AAACATGGCA 
     1741 CTCGCTGTGC GGGTGAAGTC GCTGCCTCGG CCAACAACTC CTACTGCATC GTGGGCATCG 
     1801 CATATAATGC AAAGATAGGA GGCATCCGGA TGCTGGACGG CGATGTGACC GATGTGGTGG 
     1861 AGGCCAAGTC TCTGGGCATC AGACCCAACT ACATTGACAT TTACAGCGCC AGTTGGGGAC 
     1921 CCGATGATGA CGGAAAGACA GTAGATGGAC CAGGCCGGCT GGCTAAACAA GCTTTCGAGT 
     1981 ATGGCATTAA AAAGGGTCGC CAAGGTCTGG GCTCCATTTT TGTTTGGGCC TCTGGGAATG 
     2041 GTGGGAGAGA AGGGGACCAC TGCTCCTGTG ATGGCTACAC CAACAGCATC TACACCATCT 
     2101 CCGTAAGCAG CACCACTGAG AACGGCCACA AACCCTGGTA CCTGGAAGAA TGCGCTTCCA 
     2161 CGTTGGCTAC CACCTACAGC AGCGGGGCCT TCTATGAACG GAAGATCGTC ACCACAGATC 
     2221 TGCGTCAGCG CTGCACCGAC GGCCACACTG GGACATCAGT CTCAGCCCCC ATGGTGGCTG 
     2281 GTATCATTGC CTTGGCTCTA GAAGCAAACA ACCAGTTGAC CTGGAGGGAT GTGCAGCACC 
     2341 TGCTGGTGAA GACATCACGG CCAGCTCATC TGAAGGCGAG CGACTGGAAA GTCAACGGAG 
     2401 CTGGGCATAA AGTTAGCCAT CTCTATGGAT TTGGCTTGGT GGATGCAGAA GCTCTGGTCC 
     2461 TGGAGGCGAG GAAGTGGACG GCAGTGCCCT CCCAGCACGT ATGCGTGGCC ACCGCAGACA 
     2521 AAAGGCCCAG GAGCATCCCC ATAGTGCAGG TGCTACGGAC CACAGCCCTG ACCAACGCCT 
     2581 GTGCGGACCA CTCAGACCAG CGTGTGGTGT ACCTGGAGCA TGTGGTTGTC CGAATCTCTA 
     2641 TCTCACACCC ACGCCGGGGC GATCTCCAGA TCCACCTGAT TTCTCCCTCT GGAACCAAGT 
     2701 CTCAACTTTT GGCAAAGAGA TTGCTGGATT TTTCCAATGA GGGGTTCACA AACTGGGAAT 
     2761 TCATGACCGT CCACTGCTGG GGAGAAAAGG CTGAAGGGGA GTGGACTCTG GAAGTGCAGG 
     2821 ATATACCATC GCAGGTCCGA AACCCAGAGA AACAAGGAAA GCTGAAAGAA TGGAGCCTCA 
     2881 TTTTATATGG CACCGCAGAG CACCCATATC GCACCTTCAG CTCCCATCAG TCTCGCTCAC 
     2941 GGATGCTGGA GCTCTCCGTC CCGGAGCAGG AGCCTCCCAA GGCTGCTGGA CAACCACCCC 
     3001 AGGCAGAGAC TCCAGAAGAC GAGGAAGAGT ACACAGGTGT GTGCCATCCC GAGTGTGGTG 
     3061 ACAAAGGCTG TGATGGTCCC AATGCAGACC AGTGCTTGAA CTGTGTCCAC TTCAGCCTGG 
     3121 GGAACTCCAA GACAAACAGG AAGTGTGTGA GCGAGTGCCC CTTGGGCTAC TTTGGGGATG 
     3181 CAGCTGCAAG ACGCTGCCGT CGCTGCCATA AGGGATGTGA GACGTGCACG GGCAGGAGCC 
     3241 CAGCACAGTG CCTGTCTTGT CGCCGTGGGT TCTATCACCA CCAGGAGACG AACACGTGTG 
     3301 TGACCCTCTG TCCTGCCGGA CTTTATGCTG ATGAAAGTCA GAGACTCTGC CTCAGGTGCC 
     3361 ACCCGAGCTG TCAGAAGTGC GTGGATGAAC CTGAGAAGTG CACTGTGTGT AAGGAGGGAT 
     3421 TCAGCCTGGC ACGGGGCAGC TGCATTCCGG ACTGTGAACC GGGTACCTAC TTCGATTCAG 
     3481 AGCTCGTCAA ATGTGGGGAA TGCCATCACA CCTGCCGGAC CTGTGTGGGG CCCAGCAGAG 
     3541 AAGAGTGTAT TCACTGTGCA AAAAGCTTCC ACTTCCAAGA CTGGAAATGT GTGCCGGCCT 
     3601 GCGGTGAGGG CTTCTACCCG GAGGAGATGC CTGGCTTACC CCACAAAGTG TGTCGAAGAT 
     3661 GTGAGGAGAA CTGCCTGAGC TGCGAGGGCT CCAGTAGGAA CTGCAGCAGA TGTAAAGCCG 
     3721 GCTTCACACA GCTGGGAACC TCCTGCATCA CCAACCACAC GTGCAGCAAT GCCGATGAGA 
     3781 CCTTCTGCGA GATGGTGAAG TCCAATCGGC TCTGCGAACG GAAGCTCTTC ATCCAGTTTT 
     3841 GCTGCCGCAC CTGCCTCCTG GCTGGGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3901 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3961 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4021 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4081 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4141 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4201 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4261 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4321 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4381 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4441 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4501 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4561 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4621 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4681 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4741 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4801 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4861 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4921 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4981 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5041 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5101 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5161 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5221 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5281 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5341 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5401 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5461 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5521 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5581 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5641 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5701 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5761 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5821 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5881 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5941 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6001 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6061 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6121 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6181 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6241 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6301 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6361 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6421 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6481 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6541 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6601 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6661 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6721 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6781 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6841 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6901 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6961 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7021 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7081 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7141 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7201 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7261 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7321 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7381 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7441 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7501 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7561 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7621 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7681 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//