LOCUS       MR231409                7713 bp    DNA     circular  SYN 
DEFINITION  Ganab (myc-DDK-tagged) - Mouse alpha glucosidase 2 alpha neutral subunit (Ganab), transcript variant 2
ACCESSION   NM_001293621
VERSION     NM_001293621.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="Ganab"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAATA GCGGCAGTGG CGGCGCGTAG GAGGCGGTCT TGGTTAAGTT 
     1081 TGGTGCTGGC ATACTTAGGG GTCTGTCTGG GGATTACACT TGCTGTGGAT AGAAGCAACT 
     1141 TTAAGACCTG TGATGAGAGT TCCTTTTGCA AACGGCAGCG AAGCATTCGG CCAGGCCTCT 
     1201 CTCCTTACCG TGCCTTGCTG GACACTCTGC AGCTTGGTCC TGATGCTCTT ACAGTCCATC 
     1261 TGATCCATGA AGTCACCAAG GTGCTGCTTG TGCTGGAGCT CCAGGGCCTT CAGAAGAACA 
     1321 TGACTCGGAT CAGGATCGAT GAGCTAGAGC CCCGGCGGCC TCGATACCGA GTGCCAGATG 
     1381 TTTTAGTGGC TGACCCCCCC ACAGCTAGGC TTTCAGTCTC TGGCCGTGAT GACAACAGTG 
     1441 TGGAGCTAAC AGTGGCTGAG GGACCCTACA AAATCATTTT GACAGCACAG CCATTCCGCC 
     1501 TTGACCTGCT AGAAGATCGA AGCCTCCTGC TCAGTGTCAA TGCCCGAGGA CTTATGGCCT 
     1561 TTGAGCACCA GAGGGCCCCC AGGGTCCCGC AAGAATCAAA AGACCCAGCT GAAGGCAATG 
     1621 GAGCCCAGCC TGAAGCAACA CCTGGGGATG GTGACAAGCC AGAGGAGACC CAGGAAAAGG 
     1681 CTGAGAAGGA TGAGCCAGGA GCCTGGGAGG AGACATTCAA AACACATTCT GACAGCAAGC 
     1741 CTTATGGCCC CACGTCTGTA GGTTTGGACT TTTCTCTGCC AGGAATGGAA CATGTGTATG 
     1801 GGATCCCTGA GCATGCTGAC AGCCTGAGAC TGAAGGTCAC TGAGGGCGGT GAGCCGTACC 
     1861 GCCTGTACAA TTTGGATGTG TTCCAGTATG AGCTGAACAA CCCCATGGCT CTATATGGGT 
     1921 CTGTGCCTGT GCTCCTGGCA CACAGCTTTC ATCGAGACCT GGGCATCTTC TGGCTTAATG 
     1981 CTGCTGAGAC TTGGGTTGAT ATATCCTCCA ACACGGCTGG GAAGACCCTG TTTGGGAAGA 
     2041 TGCTTGATTA CCTGCAGGGC TCTGGGGAGA CTCCACAGAC AGACATTCGT TGGATGTCAG 
     2101 AGAGTGGCAT TATTGATGTT TTCCTAATGC TTGGCCCTTC GGTTTTTGAT GTCTTTAGGC 
     2161 AGTATGCTAG TCTCACAGGG ACCCAGGCAT TGCCCCCACT CTTCTCCCTC GGCTATCACC 
     2221 AGAGTCGCTG GAACTACCGG GATGAGGCTG ATGTTTTGGA AGTGGATCAG GGTTTTGATG 
     2281 ATCACAACAT GCCTTGTGAT GTCATTTGGT TGGACATTGA ACATGCTGAT GGCAAGCGGT 
     2341 ACTTCACTTG GGACCCCACC CGATTTCCTC AGCCCCTCAA TATGCTTGAG CACTTGGCTT 
     2401 CCAAGAGGCG GAAGCTGGTG GCCATTGTGG ACCCCCACAT CAAGGTAGAC TCTGGCTACC 
     2461 GAGTTCACGA AGAATTGCGA AACCATGGGC TGTATGTTAA AACTCGGGAT GGCTCTGATT 
     2521 ACGAGGGCTG GTGCTGGCCA GGCTCAGCTA GTTACCCTGA CTTCACTAAT CCAAGGATGA 
     2581 GGGCCTGGTG GTCTAACATG TTCAGCTTTG ACAATTATGA GGGTTCAGCT CCTAATCTTT 
     2641 ATGTTTGGAA TGACATGAAT GAACCGTCTG TGTTCAATGG TCCTGAGGTC ACCATGTTGA 
     2701 AGGATGCTGT GCATTATGGA GGCTGGGAGC ACCGGGACAT CCATAACATC TATGGCTTAT 
     2761 ATGTGCACAT GGCGACTGCT GATGGGCTAA TACAGCGCTC TGGGGGCATA GAGCGTCCCT 
     2821 TTGTCCTGAG TAGGGCTTTC TTCTCAGGCT CCCAGCGCTT TGGAGCTGTG TGGACAGGGG 
     2881 ACAACACTGC CGAATGGGAT CATTTGAAGA TCTCTATCCC TATGTGTCTC AGCCTGGCAC 
     2941 TGGTGGGGCT TTCCTTCTGT GGAGCGGATG TGGGTGGCTT CTTCAAGAAC CCAGAGCCAG 
     3001 AGCTGCTTGT GCGCTGGTAC CAAATGGGTG CCTACCAGCC GTTCTTTCGG GCTCATGCCC 
     3061 ACTTGGACAC TGGGCGGCGA GAGCCATGGC TGTTAGCGTC TCAATACCAA GATGCAATCC 
     3121 GAGATGCCTT GTTCCAGCGA TATTCTTTGC TGCCCTTCTG GTATACCCTC TTCTATCAAG 
     3181 CTCACAAGGA AGGGTTTCCT GTCATGAGGC CCCTCTGGGT ACAGTATCCT GAGGATATGT 
     3241 CTACCTTCAG TATAGAGGAT CAGTTCATGC TTGGTGATGC ACTCCTTATT CACCCTGTAT 
     3301 CGGATGCTGG GGCCCACGGA GTGCAGGTCT ATTTGCCTGG CCAAGAAGAA GTGTGGTATG 
     3361 ACATTCAGAG CTATCAGAAG CATCATGGGC CCCAGACCTT GTATCTGCCA GTAACTTTGA 
     3421 GCAGTATCCC CGTGTTCCAG CGTGGCGGAA CCATTGTGCC TCGATGGATG CGTGTGAGGC 
     3481 GCTCTTCAGA CTGTATGAAG GACGATCCTA TCACTCTCTT TGTTGCTCTC AGTCCCCAGG 
     3541 GTACTGCCCA AGGAGAGCTC TTTCTAGATG ATGGACACAC CTTTAACTAT CAGACTCGCC 
     3601 ATGAGTTCCT GTTGCGGCGG TTCTCTTTCT CTGGCAGCAC ACTGGTCTCT AGTTCAGCAG 
     3661 ACCCCAAAGG CCACCTTGAG ACACCTATTT GGATTGAGCG AGTAGTCATC ATGGGGGCTG 
     3721 GAAAGCCAGC AGCTGTGGTG CTCCAGACCA AAGGATCCCC TGAAAGTCGC CTGTCCTTCC 
     3781 AGCATGACCC TGAGACCTCA GTGCTGATAT TGCGTAAACC TGGTGTCAGC GTGGCATCCG 
     3841 ACTGGAGTAT TCATCTTCGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//