LOCUS       MR231407                7713 bp    DNA     circular  SYN 
DEFINITION  Pld2 (myc-DDK-tagged) - Mouse phospholipase D2 (Pld2), transcript variant 1
ACCESSION   NM_001302475
VERSION     NM_001302475.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="Pld2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGTAACC CAGAAGAACC TCTTTCCCTA TGGGGACTAT CTGAACTCCA 
     1081 GCCAGTTGCA CATGGAGCCA GATGAGGTTG ACACTCTGAG GGAAGGAGAG GATCCAGCTG 
     1141 ATCGAATGCA TCCCTATCTG GCCATCTATG ACCTTCAGCC TCTGAAAGCA CACCCCTTGG 
     1201 TGTTCGCCCC TGGGGTCCCT GTTATAGCCC AGGTGGTGGG CACCGAAAGA TACACCAGCG 
     1261 GATCCAAGGT GGGAACCTGT ACTCTATATT CTGTTCGCTT GACGCATGGT GACTTTACCT 
     1321 GGACAACCAA GAAGAAGTTC CGACACTTTC AGGAGCTGCA TCGGGACCTC CAGAGACACA 
     1381 AAGTCTTGAT GAGTCTGCTC CCTTTGGCTC GCTTTGCTGT GACCCATTCT CCAGCCCGAG 
     1441 AGGCAGCCGC CGAGGATATA CCCTCCCTAC CCCGAGGAGG TTCTGAGGGC TCTGCCAGAC 
     1501 ACACAGCCAG CAAACAGAAA TACTTGGAAA ATTACCTCAA CCGCCTCCTG ACCATGTCTT 
     1561 TCTATCGCAA TTACCACGCC ATGACAGAAT TTCTGGAAGT CAGTCAACTT TCCTTTATCC 
     1621 CAGACCTTGG CTCCAAAGGA CTGGAAGGGG TGATCCGGAA GCGCTCGGGC GGGCATCGAG 
     1681 TTCCCGGCTT CACCTTCTGT GGCCGAGACC AAGTTTGTTA TCGATGGTCC AAGAGGTGGC 
     1741 TGGTGGTGAA GGACTCCTTC CTGCTGTACA TGCGCCCGGA GACCGGCGCC ATCTCATTTG 
     1801 TTCAGCTTTT TGACCCTGGC TTTGAGGTCC AGGTCGGAAA AAGGAGCACA GAGACGCGGT 
     1861 ATGGGGTGAG GATCGACACC TCCCACAGGT CCCTGATTCT CAAATGCAGC AGCTACCGGC 
     1921 AGGCACGGTG GTGGGGCCAG GAGATCACGG AGCTGGCACA GGGTTCGGGC AGAGATTTTC 
     1981 TACAGCTACA TCAGCATGAC AGCTATGCCC CACCCCGGCC CGGCACCCTG GCCCGGTGGT 
     2041 TTGTGAATGG GGCAGGTTAC TTTGCTGCTG TGGCAGATGC CATCCTGCGA GCTCAAGAGG 
     2101 AGATTTTCAT CACAGACTGG TGGTTGAGTC CTGAAATTTA CCTGAAGCGT CCAGCCCATT 
     2161 CCGACGACTG GAGACTGGAC ATTATGCTCA AGAGGAAGGC GGAAGAAGGT GTCCGAGTTT 
     2221 CCATACTGCT GTTTAAGGAA GTGGAGCTGG CCTTGGGCAT CAACAGTGGC TACAGCAAGA 
     2281 GGACGCTGAT GCTGCTGCAT CCCAACATAA AGGTGATGCG ACACCCAGAC CTTGTGACAC 
     2341 TGTGGGCTCA TCACGAGAAG CTCCTGGTGG TAGACCAAGT GGTGGCATTC TTGGGCGGGC 
     2401 TGGACCTGGC CTTCGGCCGC TGGGATGACG TGCAATACCG ACTGACTGAC CTGGGTGACC 
     2461 CCTCTGAACC TGTACATTTA CAGACTCCCA CACTAGGTTC AGACCCTGCA GCCACTCCAG 
     2521 ACCTCTCGCA TAACCAATTC TTCTGGCTGG GAAAGGACTA CAGCAACCTC ATCACCAAGG 
     2581 ACTGGGTGCA GCTGGACCGG CCTTTTGAAG ATTTCATCGA CAGGGAGACC ACACCCAGGA 
     2641 TGCCATGGAG GGATGTTGGA GTGGTTGTAC ACGGAGTAGC TGCCAGGGAC CTTGCCCGGC 
     2701 ACTTCATCCA GCGCTGGAAT TTCACCAAGA CCACCAAGGC CAGGTATAAG ACACCTTTGT 
     2761 ACCCCTACCT GCTGCCCAAG TCCACCAGCA CTGCAAACAA TCTCCCCTTC ATGATCCCAG 
     2821 GCGGGCAGTG TGCCACTGTG CAGGTCTTGA GGTCTGTGGA TCGATGGTCA GCAGGGACAT 
     2881 TGGAGAACTC CATCCTCAAT GCCTACCTAC ATACCATTCG AGAGAGCCAG CACTTTCTCT 
     2941 ACATTGAGAA TCAGTTCTTC ATTAGCTGCT CAGATGGGCG AACAGTTCTG AACAAGGTGG 
     3001 GCGATGAGAT TGTGGACAGA ATCCTGAAGG CTCACGAACA GGGGCAGTGT TTCCGAGTCT 
     3061 ACTTGCTTCT GCCTTTGCTC CCTGGCTTTG AGGGGGACAT CTCCACAGGG GGTGGTAACT 
     3121 CCATCCAGGC TATTCTGCAC TTCACCTACA GCCTCTGCCT CCTTCACCCT TTCTTCTCTC 
     3181 TCAGGACCCT GTGTCGTGGG GAACATTCAA TCCTACATCG TCTCAAAGCA GCCATGGGGA 
     3241 CTGCGTGGCG AGATTACATG TCCATCTGTG GGCTTCGCAC CCATGGAGAG CTGGGCGGGC 
     3301 ACCCAATCTC TGAGCTCATC TATATCCACA GCAAGATGCT CATTGCGGAT GACAGAACAG 
     3361 TCATCATTGG TTCTGCGAAC ATCAATGACA GGAGCTTGCT GGGGAAGCGT GACAGTGAGC 
     3421 TAGCCATCCT GATCAAGGAC ACAGAAATGG AACCATCCCT CATGGATGGG GTGGAGTACC 
     3481 AGGCAGGCAG ATTTGCCTTG AGTTTGCGGA AGCACTGTTT CAGTGTGATT CTTGGGGCAA 
     3541 ATACCTGGCC AGACCTGGAT CTCCGAGACC CTGTCTGTGA TGACTTCTTC CAGCTGTGGC 
     3601 AAGAAACAGC GGAGAACAAT GCCACCATCT ATGAGCAGAT CTTCCGCTGC CTGCCGTCCA 
     3661 ATGCTACCCG TTCCCTGCGG GCTCTCCGGG AGTATGTGGC TGTGGAGTCC TTGGCTACAG 
     3721 TCAGCCCTTC TTTGGCTCAG TCTGAGCTTG CCCACATCCA GGGCCACCTA GTTCACTTCC 
     3781 CCCTCAAGTT TCTGGAGGAC GAGTCCTTGT TGCCCCCACT GGGGAGTAAA GAAGGGATGA 
     3841 TACCTTTAGA AGTGTGGACA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//