LOCUS       MR231401                7686 bp    DNA     circular  SYN 
DEFINITION  Ano6 (myc-DDK-tagged) - Mouse anoctamin 6 (Ano6), transcript variant 1
ACCESSION   NM_001253813
VERSION     NM_001253813.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3841..3932 
                     /label="Myc-DDK"  
     misc_feature    3962..4547 
                     /label="PolyA signal"  
     misc_feature    4019..4038 
                     /label="Restriction Enzyme"  
     misc_feature    4823..5495 
                     /label="ColE1"  
     misc_feature    5925..6719 
                     /label="Kan/Neo"  
     misc_feature    6754..7056 
                     /label="SV40 ori"  
     misc_feature    7142..7619 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3816..3823 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3816 
                     /label="Ano6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGATGATG ACTAGGAAGG TCCTGCTGAA CATGGAGCTG GAGGAGGACG 
     1081 ACGATGAGGA TGGAGACATT GGTGATGTTC CTGACTCCAG GAGGCCTTTT CTGGCTCCTC 
     1141 ATACTCCTCT ACCATCCGGT CTGGTGCTGG AAAACTTTGA CCAGACAATT GTCTGCCCCA 
     1201 CCTTTGGATC ACTGGAGAAT CAGCAGGACT TCAGGACTCC AGAGTTTGAA GAATTTAACG 
     1261 GGAAGCCCGA CTCCCTCTTT TTCACCGATG GCCAGAGGCG AATCGACTTC ATCCTCGTGT 
     1321 ATGAAGATGA GAGCAAAAAG GAGAACAATA AGAAAGGGAC AAATGAGAAA CAGAAGAGGA 
     1381 AAAGACAAGC ATACGAATCT AACCTTATCT GCCATGGGCT GCAGCTGGAA GCAACAAGAT 
     1441 CTGTTTCTGA TGACAAGCTT GTGTTCGTAA AAGTGCACGC GCCCTGGGAA GTGCTGTGCA 
     1501 CCTATGCTGA GATCATGCAC ATCAAACTCC CGCTAAAGCC AAACGACCTG AAAACGCGCT 
     1561 CGCCCTTTGG CAACCTCAAC TGGTTCACCA AGGTCCTCCG GGTGAACGAG AGTGTCATCA 
     1621 AGCCAGAGCA GGAGTTCTTC ACTGCCCCTT TTGAGAAGAG CCGGATGAAT GATTTCTACA 
     1681 TCCTCGATAG AGATTCCTTC TTCAACCCTG CCACCAGAAG CCGCATTGTT TATTTCATCC 
     1741 TCTCTCGGGT CAAATACCAA GTGATGAACA ACGTTAACAA ATTTGGGATT AATAGACTGG 
     1801 TCAGCTCTGG AATCTACAAA GCAGCGTTTC CTCTGCACGA CTGCAGATTC AACTATGAGT 
     1861 CGGAGGACAT CAGTTGTCCT AGCGAGCGTT ACCTCCTGTA CAGAGAATGG GCTCACCCTC 
     1921 GGAGTATATA CAAGAAGCAG CCCTTGGATC TTATCAGGAA GTATTACGGC GAGAAGATTG 
     1981 GAATCTACTT TGCTTGGCTG GGCTATTACA CGCAGATGCT CCTTCTCGCA GCTGTGGTGG 
     2041 GCGTGGCCTG CTTCCTCTAT GGATATCTTG ATCAGGATAA CTGCACTTGG AGCAAAGAGG 
     2101 TCTGTGATCC TGACATCGGT GGCCAGATCC TGATGTGCCC CCAGTGTGAC AGGCTGTGTC 
     2161 CGTTCTGGAG GCTGAATATC ACCTGTGAAT CTTCTAAGAA ATTGTGCATC TTTGACAGTT 
     2221 TTGGAACCCT GATCTTCGCT GTATTTATGG GAGTGTGGGT TACATTGTTT TTGGAGTTTT 
     2281 GGAAGCGGCG CCAGGCAGAG CTCGAATATG AATGGGACAC CGTTGAGCTA CAGCAAGAGG 
     2341 AACAGGCCCG GCCAGAGTAT GAGGCCCAGT GCAATCACGT GGTGATAAAC GAGATCACTC 
     2401 AGGAGGAGGA GCGCATCCCA TTTACCACCT GTGGGAAGTG CATCCGGGTC ACGCTGTGTG 
     2461 CGAGCGCCGT CTTCTTCTGG ATCCTGCTCA TCATCGCCTC TGTGATCGGG ATCATTGTCT 
     2521 ACAGGCTGTC CGTGTTCATC GTATTTTCCA CAACGCTCCC CAAGAACCCC AACGGGACAG 
     2581 ACCCGATCCA GAAGTACCTG ACCCCACAGA TGGCCACATC CATCACAGCC TCCATCATCA 
     2641 GCTTCATCAT CATCATGATC CTCAACACGA TCTACGAGAA GGTGGCCATC ATGATCACCA 
     2701 ACTTCGAGCT CCCAAGGACC CAGACGGATT ATGAGAACAG CCTGACCATG AAGATGTTCT 
     2761 TGTTCCAGTT TGTCAACTAT TACTCCTCAT GCTTCTACAT CGCATTCTTC AAGGGCAAAT 
     2821 TCGTGGGCTA TCCCGGGGAC CCAGTGTACT TGCTGGGCAA ATACAGAAGC GAAGAGTGTG 
     2881 ACCCGGGGGG CTGCCTCCTT GAACTGACCA CACAGCTGAC GATCATCATG GGGGGAAAGG 
     2941 CAATCTGGAA CAACATACAA GAAGTCTTGC TCCCATGGGT TATGAATCTA ATTGGACGAT 
     3001 ATAAAAGAGT CTCGGGATCA GAAAAGATAA CCCCACGATG GGAACAGGAT TACCATCTGC 
     3061 AGCCCATGGG CAAGCTGGGA TTGTTCTATG AGTATCTTGA AATGATTATT CAGTTTGGGT 
     3121 TCGTCACTTT ATTTGTGGCC TCTTTCCCAC TGGCTCCCCT GCTGGCTCTG GTGAACAATA 
     3181 TTTTGGAAAT AAGAGTGGAC GCGTGGAAGC TCACAACCCA GTTTAGGCGC ATGGTGCCCG 
     3241 AAAAAGCCCA GGATATCGGC GCGTGGCAGC CCATCATGCA AGGAATAGCC ATCCTGGCCG 
     3301 TGGTGACCAA CGCCATGATC ATTGCTTTCA CATCAGACAT GATCCCCCGC CTGGTGTATT 
     3361 ATTGGTCTTT CTCTATCCCT CCCTACGGGG ACCACACTTA TTACACCATG GACGGCTACA 
     3421 TCAACAACAC TCTCTCTGTC TTCAACATCA CGGACTTCAA GAACACAGAC AAAGAAAACC 
     3481 CATACATTGG GCTTGGTAAC TATACCTTGT GCAGGTACCG TGACTTCCGA AACCCACCTG 
     3541 GGCACCCACA AGAGTATAAA CACAACATCT ACTATTGGCA CGTGATTGCC GCCAAGCTGG 
     3601 CCTTCATCAT TGTCATGGAG CACATCATCT ACTCTGTGAA GTTCTTCATT TCCTACGCAA 
     3661 TTCCAGATGT GTCCAAAATC ACGAAAAGCA AGATCAAGAG GGAGAAATAC CTCACGCAGA 
     3721 AGTTGCTTCA CGAGAGTCAC CTCAAAGACC TGACCAAAAA CATGGGGATC ATCGCCGAGA 
     3781 GGATAGGAGG AACGGTTGAC AACAGCGTGC GGCCAAAACT CGAAAGCGGA CCGACGCGTA 
     3841 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3901 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3961 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4021 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4081 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4141 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4201 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4261 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4321 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4381 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4441 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4501 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4561 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4621 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4681 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4741 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4801 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4861 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4921 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4981 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5041 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5101 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5161 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5221 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5281 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5341 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5401 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5461 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5521 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5581 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5641 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5701 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5761 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5821 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5881 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5941 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6001 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6061 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6121 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6181 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6241 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6301 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6361 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6421 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6481 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6541 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6601 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6661 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6721 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6781 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6841 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6901 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7021 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7081 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7141 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7261 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7321 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7381 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7441 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7561 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7621 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7681 AATTTT
//