LOCUS       MR231353                7584 bp    DNA     circular  SYN 
DEFINITION  Usp33 (myc-DDK-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 3
ACCESSION   NM_001252486
VERSION     NM_001252486.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3741..3832 
                     /label="Myc-DDK"  
     misc_feature    3862..4447 
                     /label="PolyA signal"  
     misc_feature    3919..3938 
                     /label="Restriction Enzyme"  
     misc_feature    4723..5395 
                     /label="ColE1"  
     misc_feature    5825..6619 
                     /label="Kan/Neo"  
     misc_feature    6654..6956 
                     /label="SV40 ori"  
     misc_feature    7042..7519 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3723..3729 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3723 
                     /label="Usp33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGACTTTT CGAAATCATT GTCCACATTT GGATTCAGTT GGTGAAATAA 
     1081 CGAAAGAGGA CTTGATACAG AAATCTCTCG GCGCTTGTCA GGACTGTAAA GTCAGAGGAC 
     1141 CAAACCTGTG GGCGTGCCTG GAGAATAGGT GCTCCTATGT TGGCTGCGGG GAGTCGCAAG 
     1201 TGGACCACAG CACCATACAC TCGCAGGAGA CAAAGCATTA TCTAACTGTG AACCTCACCA 
     1261 CTCTTCGAGT ATGGTGTTAT GCTTGCAGCA AAGAAGTATT TTTGGATAGA AAATTAGGAA 
     1321 CTCCTCCTTC ATTACCTCAT GTAAGACAGC CTCAACAAAC ACAAGAAAAC AGTGTCCAGG 
     1381 ATTTTAAAAT TCCCAGTAAT CCAGCATTGA AAACCCCCAT GGTTGCTGTG TCTGAAGATC 
     1441 TGGATATAGA AGTGGAAGAG GAAGACGAGC TGAAGGCTAG AGGCCTGACA GGTTTGAAAA 
     1501 ACATTGGAAA TACTTGTTAT ATGAACGCGG CGCTGCAGGC TCTTTCTAAC TGCCCACCTT 
     1561 TGACACAGTT CTTTCTTGAT TGTGGAGGAC TGGCTAGAAC AGATAAGAAA CCAGCAATTT 
     1621 GTAAAAGTTA TCTCAAACTA ATGACCGAGT TGTGGCACAA GAGCAGGCCA GGATCTGTTG 
     1681 TGCCGGCTAA TCTGTTCCAA GGAATTAAAA CTGTAAATCC AACTTTTCGA GGTTATTCTC 
     1741 AGCAGGATGC CCAGGAATTC CTTCGCTGTC TAATGGACCT GCTTCATGAG GAGCTGAAGG 
     1801 AGCAGGTCAT GGAAATGGAG GAAGAGCCTC AAACACTAAC TTCTGAGGAG ACGGTGGAGG 
     1861 AAGAGAAGAG CCAGTCAGAT GTGGATTTTC AGTCGTGCGA GTCTTGTAGC AGCAGCGAGA 
     1921 AAGCAGAAAA TGAGAGTGGC TCCAAAGGCT TTCCTGAGGA CAGCAATGAG ACCACCATGC 
     1981 TCATCCAGGA CGAGGATGAC CTGGAGATGG CCAAAGACTG GCAGAAAGAG AAGGTGTGCA 
     2041 ATAAGATCAA CAAGGCAAAT GCCGATGTAG AACTGGACAA AGACAGGGAC ACAGTGTGTG 
     2101 AAACAGTTGA CCTAAACAGC CAGGAGACCG TCAAAGTGCA GATACACGGC AGAGCCTCAG 
     2161 AATCTATCAC TGATGTCCAT CTGAATGACC TAGCTACGTC ACAGATCCTT CCTTCAAATG 
     2221 AAAGTGTTAG TCCACGGTTA TCAGCAAGCC CTCCTAAGCT AGGCAGTCTG TGGCCAGGAC 
     2281 TGTCGCCTCC ACACAAGAAA GCTCAGTCTA CATCTGCAAA GAGGAAAAAG CAGCATAAGA 
     2341 AATACAGAAG TGTCATCTCC GACATATTCG ATGGAACAGT CATTAGCTCG GTACAGTGTC 
     2401 TGACGTGCGA TAGGGTGTCT ATAACCCTCG AGACCTTTCA GGATCTGTCC TTGCCGATTC 
     2461 CTGGCAAGGA GGACCTGGCT AAGCTGCACT CCTCCAGTCA CCCAACTATA GTCAAAGCAG 
     2521 GGTCATGTGG TGAAGCGTAC GCCCCGCAGG GGTGGATAGC TTTCTTCATG GAGTATGTGA 
     2581 AGAGCTGGTT TTGGGGTCCA GTAGTTACCT TGCAAGATTG TCTTGCTGCC TTCTTCGCCA 
     2641 GAGATGAACT TAAAGGTGAC AACATGTACA GTTGTGAAAA ATGCAAAAAG TTGAGGAATG 
     2701 GAGTAAAGTT TTGTAAAGTA CAGAAGTTTC CTGAGATTTT GTGTATCCAC CTTAAAAGAT 
     2761 TTCGACATGA ACTGATGTTT TCCACCAAAA TTAGCACCCA TGTTTCCTTC CCCCTGGAAG 
     2821 GCCTCGATCT TCAGCCATTT CTTGCAAAGG ACAGCCCAGC TCAGATTGTG ACATATGATC 
     2881 TCCTGTCAGT CATCTGTCAC CATGGGACTG CAAGTAGTGG GCACTACATT GCCTACTGCC 
     2941 GAAACAATTT AAATAACCTG TGGTATGAGT TTGATGACCA GAGCGTCACT GAAGTTTCAG 
     3001 AGTCCACTGT ACAGAATGCC GAGGCCTACG TCCTTTTCTA CAGGAAGAGC AGTGAAGAGG 
     3061 CACAAAAGGA GAGGCGGAGG ATATCAAATT TGTTGAACAT CATGGAACCT AGCCTCCTTC 
     3121 AGTTCTACAT ATCTCGACAG TGGTTGAATA AATTTAAGAC CTTTGCTGAA CCTGGCCCTA 
     3181 TTTCAAATAA TGATTTTCTC TGTATCCATG GAGGTATTCC TCCACGAAAA GCGAGTTATA 
     3241 TTGAAGACTT AGTTTTGATG CTGCCTCAGA ACATTTGGGA TAACCTCTAT AGCAGGTATG 
     3301 GAGGAGGGCC TGCTGTCAAC CATCTCTACA TCTGCCACAC CTGCCAAATT GAGTTAGAGA 
     3361 AGATTGAAAA ACGAAGAAAA ACCGAATTGG AAATTTTTAT TCGGCTCAAC AGAGCATTTC 
     3421 AAGAGGAGGA CTCCCCAGCT ACTTTTTACT GTATCAGCAT GCAGTGGTTT AGAGAATGGG 
     3481 AGAGTTTTGT AAAGGGTAAG GATGGAGATC CCCCAGGTCC AATCGACAAC ACTAAAATTG 
     3541 CGGTTACTAA ATGTGGCAGT GTGATGCTCA AGCAAGGAGC AGACTCTGGT CAAATTTCAG 
     3601 AAGAAACATG GAATTTCCTG CAGTCTATAT ATGGTGGGGG GCCTGAAGTT ATCCTCCGAC 
     3661 CTCCAGTTGT TCATGTTGAC CCTGATGTAC TCCAAGCAGA GGAAAAGATT GAAGTAGAAA 
     3721 CTCGCTCTTT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3781 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3841 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3901 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3961 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4021 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4081 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4141 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4201 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4261 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4321 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4381 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4441 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4501 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4561 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4621 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4681 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4741 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4801 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4861 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4921 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4981 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5041 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5101 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5161 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5221 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5281 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5341 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5401 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5461 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5521 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5581 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5641 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5701 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5761 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5821 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5881 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5941 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6001 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6061 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6121 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6181 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6241 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6301 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6361 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6421 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6481 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6541 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6601 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6661 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6721 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6781 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6841 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6901 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6961 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7021 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7081 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7141 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7201 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7261 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7321 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7381 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7441 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7501 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7561 TAACAAAAAT TTAACGCGAA TTTT
//