LOCUS       MR231331                7560 bp    DNA     circular  SYN 
DEFINITION  Dag1 (myc-DDK-tagged) - Mouse dystroglycan 1 (Dag1), transcript variant 2
ACCESSION   NM_001276481
VERSION     NM_001276481.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3717..3808 
                     /label="Myc-DDK"  
     misc_feature    3838..4423 
                     /label="PolyA signal"  
     misc_feature    3895..3914 
                     /label="Restriction Enzyme"  
     misc_feature    4699..5371 
                     /label="ColE1"  
     misc_feature    5801..6595 
                     /label="Kan/Neo"  
     misc_feature    6630..6932 
                     /label="SV40 ori"  
     misc_feature    7018..7495 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3699..3705 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3699 
                     /label="Dag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGTGGAC AACTGGCTAC TGCACCCCCT CTGGGGACAG ACCTTTCTCC 
     1081 TCCTCCTGTC TGTGGCTGTG GCTCAGGCCC ACTGGCCCAG TGAACCCTCA GAGGCTGTGA 
     1141 GGGACTGGAA GAACCAGCTT GAGGCGTCCA TGCACTCAGT TCTCTCCGAC TTCCAGGAGG 
     1201 CTGTTCCCAC CGTGGTTGGC ATTCCAGACG GTACGGCTGT TGTCGGGCGC TCATTTCGAG 
     1261 TGAGCATTCC AACGGATTTA ATTGCCTCCA GTGGGGAGAT CATCAAGGTG TCTGCAGCAG 
     1321 GGAAGGAGGC CTTACCGTCT TGGCTACACT GGGACCCACA CAGTCATATT TTGGAAGGCC 
     1381 TTCCTCTTGA CACTGATAAA GGTGTGCATT ACATCTCAGT GAGTGCTGCA CGCCTGGGAG 
     1441 CCAATGGAAG CCACGTCCCC CAGACTTCCA GTGTGTTCTC TATCGAGGTC TACCCTGAAG 
     1501 ACCACAATGA GCCACAGTCT GTACGGGCAG CCTCATCAGA CCCTGGTGAG GTAGTGCCAT 
     1561 CTGCCTGTGC TGCTGATGAG CCAGTGACTG TCCTTACAGT GATTCTGGAT GCTGACCTCA 
     1621 CCAAGATGAC CCCAAAGCAA AGGATCGATC TGTTGAACAG AATGCAGAGC TTCTCAGAAG 
     1681 TAGAACTTCA CAACATGAAG TTGGTGCCTG TAGTGAATAA TAGACTATTT GACATGTCGG 
     1741 CCTTCATGGC TGGCCCAGGA AATGCAAAGA AAGTGGTAGA GAATGGGGCT CTCCTGTCCT 
     1801 GGAAACTAGG CTGCTCCTTG AACCAGAATA GCGTCCCTGA CATCCGTGGT GTAGAAACCC 
     1861 CTGCTAGGGA GGGTGCTATG TCTGCCCAAC TTGGTTATCC TGTGGTGGGT TGGCACATTG 
     1921 CCAATAAGAA GCCCACTCTC CCCAAACGAC TCCGGAGGCA GATCCACGCC ACACCTACAC 
     1981 CTGTTACTGC CATTGGACCC CCAACCACGG CCATTCAGGA GCCACCATCG CGGATAGTGC 
     2041 CTACGCCTAC ATCTCCAGCC ATTGCACCTC CAACAGAGAC CATGGCTCCT CCTGTCAGGG 
     2101 ATCCTGTTCC AGGGAAGCCC ACGGTCACCA TTCGGACGCG AGGTGCCATT ATTCAGACCC 
     2161 CAACTCTGGG CCCTATCCAG CCTACTCGGG TGTCAGAAGC TGGTACCACG GTTCCTGGCC 
     2221 AGATTCGCCC AACACTGACA ATTCCTGGCT ATGTAGAGCC CACAGCCGTT ATTACTCCTC 
     2281 CAACAACTAC CACAAAGAAG CCACGAGTGT CCACGCCAAA GCCAGCAACG CCTTCAACTG 
     2341 ATTCGTCAAC TACCACAACT CGGAGGCCAA CCAAAAAACC ACGGACACCC CGACCAGTGC 
     2401 CCCGAGTCAC CACCAAAGCA CCCATCACCA GGTTGGAGAC AGCTTCCCCA CCCACTCGAA 
     2461 TCCGTACTAC CACCAGTGGA GTGCCCCGTG GGGGAGAACC TAACCAGCGG CCAGAGCTCA 
     2521 AGAATCACAT TGACAGGGTA GATGCCTGGG TGGGAACCTA TTTTGAGGTA AAGATTCCAT 
     2581 CAGACACCTT CTATGACAAT GAGGATACCA CTACCGACAA GCTCAAGCTG ACCCTGAAGC 
     2641 TTCGAGAGCA GCAGTTAGTA GGTGAGAAAT CGTGGGTTCA GTTTAACAGC AACAGCCAGC 
     2701 TCATGTATGG CCTGCCTGAC AGCAGCCATG TGGGAAAACA TGAGTATTTT ATGCATGCCA 
     2761 CAGACAAAGG GGGCCTCTCC GCTGTGGATG CCTTCGAGAT CCATGTTCAC AAGCGCCCAC 
     2821 AAGGGGACAA GGCTCCTGCA CGGTTCAAGG CCAGGCTTGC AGGGGATCCA GCACCGGTGG 
     2881 TGAATGACAT TCACAAGAAA ATTGCTTTGG TAAAGAAGCT AGCTTTTGCT TTTGGGGATC 
     2941 GAAACTGCAG CTCCATCACC CTTCAGAACA TCACTCGGGG CTCTATCGTG GTGGAATGGA 
     3001 CCAACAACAC TCTGCCCCTG GAGCCCTGCC CCAAGGAGCA GATCATAGGG CTGAGCCGCA 
     3061 GGATTGCTGA TGAAAATGGG AAGCCTCGTC CTGCCTTCTC CAATGCTCTG GAGCCTGACT 
     3121 TTAAGGCTCT GAGTATTGCT GTGACGGGCT CTGGCAGTTG TCGGCACCTC CAGTTTATCC 
     3181 CTGTGGCACC ACCCTCTCCT GGAAGCTCAG CTGCACCAGC CACAGAGGTT CCAGACAGGG 
     3241 ACCCCGAGAA GAGCAGTGAG GACGATGTTT ACCTGCACAC CGTTATCCCA GCCGTGGTGG 
     3301 TCGCGGCCAT CCTGCTCATT GCTGGAATCA TTGCTATGAT CTGCTATCGC AAGAAGAGGA 
     3361 AGGGCAAGCT GACCCTTGAG GACCAGGCCA CCTTTATTAA GAAGGGGGTG CCTATCATCT 
     3421 TTGCGGATGA GCTGGATGAC TCTAAGCCCC CGCCCTCTTC CAGCATGCCG CTCATCTTGC 
     3481 AGGAAGAGAA GGCTCCCCTC CCACCTCCTG AGTACCCCAA CCAGAGTATG CCCGAGACCA 
     3541 CTCCTCTGAA CCAGGACACT GTGGGAGAGT ACACACCCCT GCGGGATGAG GATCCTAACG 
     3601 CACCTCCCTA TCAGCCACCC CCACCCTTCA CGGCTCCCAT GGAGGGCAAG GGCTCCCGTC 
     3661 CCAAGAACAT GACCCCATAC CGATCACCCC CTCCGTATGT TCCCCCTACG CGTACGCGGC 
     3721 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3781 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3841 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3901 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3961 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4021 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4081 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4141 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4201 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4261 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4321 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4381 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4441 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4501 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4561 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4621 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4681 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4741 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4801 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4861 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4921 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4981 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5041 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5101 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5161 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5221 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5281 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5341 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5401 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5461 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5521 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5581 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5641 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5701 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5761 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5821 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5881 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5941 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6001 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6061 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6121 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6181 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6241 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6301 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6361 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6421 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6481 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6541 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6601 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6661 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6721 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6781 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6841 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6901 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6961 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7021 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7081 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7141 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7201 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7261 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7321 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7381 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7441 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7501 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//