LOCUS       MR231309                7521 bp    DNA     circular  SYN 
DEFINITION  Hk3 (myc-DDK-tagged) - Mouse hexokinase 3 (Hk3), transcript variant 3
ACCESSION   NM_001206391
VERSION     NM_001206391.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3676..3767 
                     /label="Myc-DDK"  
     misc_feature    3797..4382 
                     /label="PolyA signal"  
     misc_feature    3854..3873 
                     /label="Restriction Enzyme"  
     misc_feature    4658..5330 
                     /label="ColE1"  
     misc_feature    5760..6554 
                     /label="Kan/Neo"  
     misc_feature    6589..6891 
                     /label="SV40 ori"  
     misc_feature    6977..7454 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3651..3658 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3651 
                     /label="Hk3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCATT GGGCCTTCTG GCCTGCATCC TGGAGAAAGA GCCTCAGTCT 
     1081 GCCCACACGA GGGCGTGCCA AGGCCCTCAG GTAGCTTAGA ACTGGAATGC TTGCAGCAAT 
     1141 TCAAGGTGAC CAGGACACAG TTGCAGCAGA TCCAAGCCAG TCTCCTGTGC TCCATGGAGC 
     1201 AGGCACTGAA GGGACAGGAC AGTCCCGCTC CTTCTGTCCG GATGCTGCCC ACATACGTGA 
     1261 GGTCCACACC ACACGGCACC GAGCAAGGGG ATTTCCTGGT GCTGGAGCTG GGGGCCACAG 
     1321 GAGCATCACT ACGTGTGTTG TGGGTAACAC TGACAGGCAC CAAAGAATGC CGGGTGGAGC 
     1381 CCAGGAGCCG GGAGTTTGTG ATTCCTCAAG AGGTGATCCT AGGTGCTGGC CAGCAGCTCT 
     1441 TTGACTTTGC TGCCCGATGC CTCTCTGAAT TCCTGGATGC ATACCCCGTG GAGAATCAGG 
     1501 GTCTGAAGCT TGGGTTCAAT TTCTCTTTTC CTTGTCACCA GACAGGCTTG GACAGGAGCA 
     1561 CCCTCATTTC CTGGACAAAA GGTTTTAGGT GCAGCGGCGT GGAAGGCCAG GATGTGGTCC 
     1621 AGTTGCTAAG AGATGCCATT CAGAGGCAGG GGACCTACAG GATTGATGTG GTAGCCATGG 
     1681 TGAATGACAC GGTGGGCACC ATGATGGGCT GTGAGCTGGG CACCAGGCCG TGTGAAGTCG 
     1741 GGCTGATTGT AGACACCGGT ACCAACGCGT GCTACATGGA GGAGGCGCGG CACGTGGCCG 
     1801 CTCTGGATGA GGACCGCGGC CGCACCTGTG TCAGCATCGA GTGGGGCTCC TTCTATGACG 
     1861 AAGATGCTCT AGGGCCAGTA CTGACCACCT TCGACTCTGC CCTGGACCGC GAGTCCCTGA 
     1921 CTCCTGGTGC TCAAAGGTTT GAGAAGATGA TTGGAGGCCT TTACCTGGGT GAGCTGGTGA 
     1981 GGCTGGTTCT GGTCCACTTG ACCCAGCACG GGGTCCTCTT TGATGGCTGC GCCTCTCCTG 
     2041 CGTTGCTGAG TCAAGGCTGT ATCCTCCTGG ACCACGTGGC TGAGATGGAG GACACCGCCA 
     2101 CTGGGACAGC CCGCGTCCAC ACCATCCTGC AGGACTTGGG TCTGAGCCCT CGGGCCTCAG 
     2161 ATGCTGAACT GGTGCAATAT GTGTGCGTGG CTGTGTGCAC ACGGGCTGCC CAGCTCTGTG 
     2221 CGGCTGCCCT GGCTGCAGTC CTCTCCCGCC TCCAGCACAG CAGGGAGCAG CAGACATTGC 
     2281 AAGTGGCTGT GGCCACTGGA GGGCGAGTGT TTGAACGACA CCCCAGGCGC ATCCTGGAAG 
     2341 AGACCCTGGC ACCATTTCAG CTGACCTTGG AGCAGATGAC AGTGGTGCAG GCACAAATGC 
     2401 GGGAAGCCAT GATCAGGGGG CTTCAAGGAG AGGCCTCCTC CCTCCGCATG CTGCCCACTT 
     2461 ACGTCCGAGC GACGCCCGAC GGCAGCGAGC GAGGTGATTT CCTGGCCTTG GACCTAGGGG 
     2521 GCACCAACTT CCGGGTCCTG TTGGTACGAG TGGCGGAGGG CAGTGTGCAG ATCATCAACC 
     2581 AGGTCTACTC CATTCCTGAG TGTAGAGCCC AGGGCTCTGG ACAGAAGCTC TTTGACCATA 
     2641 TTGTGGACTG CATCGTGGAC TTCCAGAAGA GGCAGGGCCT TAGTGGACAG AGCCTACCCC 
     2701 TGGGTTTCAC CTTCTCTTTC CCATGCAAGC AGCTTGGCCT GGACCAGGGC ATCCTCCTGA 
     2761 ATTGGACTAA GGGGTTCAAT GCATCAGGCT GTGAGGGCCA GGATGTTGTG TATCTATTAC 
     2821 GGGAAGCCAT TAGGCGCAGA CAGGCAGTGG AGCTGAATGT GGTTGCCATT GTCAATGACA 
     2881 CGGTGGGGAC CATGATGTCC TGTGGCTATG ATGATCCCCG TTGTGAGATG GGCCTCATTG 
     2941 TCGGAACCGG CACCAACGCC TGTTACATGG AAGAGCTCCG CAATGTGGCA AGTGTGCCTG 
     3001 GGGACTCGGG CCTCATGTGC ATCAACATGG AGTGGGGTGC CTTTGGGGAT GATGGCTCAC 
     3061 TGGGCACACT CAGCACCCGC TTTGACACCA GTGTGGACCA GGCATCCATC AATCCAGGCA 
     3121 AACAGAGGTT TGAGAAAATG ATCAGCGGTA TGTACTTGGG GGAGATCGTC CGCCATATCC 
     3181 TCCTGCACTT AACCAATCTC GGAGTTCTCT TCCGAGGCCA GAAGACTCAA TGCCTTCAGG 
     3241 CCAGGGACAT CTTCAAGACT AAGTTCCTCT CTGAGATCGA AAGTGATAGC CTGGCCCTGC 
     3301 GTCAGGTCCG AGCCATCCTG GAAGATCTGG GGCTGACTCT GACATCTGAT GACGCCTTGA 
     3361 TGGTCCTGGA AGTGTGCCAG GCTGTGTCTC GCAGGGCTGC CCAACTCTGT GGGGCAGGTG 
     3421 TGGCTGCCGT GGTGGAGAAG ATTCGGGAGA ACCGGGGCCT GCAGGAGCTG ACAGTGTCTG 
     3481 TGGGAGTAGA TGGGACACTC TACAAGCTAC ATCCTCACTT CTCCAAGCTG GTGTCGGCAA 
     3541 CAGTCCGGAA GCTAGCCCCT CAGTGCACCG TCACCTTTTT GCAGTCGGAG GATGGGTCTG 
     3601 GCAAAGGCGC AGCTTTGGTT ACTGCTGTTG CTTGTCGCCT TACCCAGATG GCCCACGTCA 
     3661 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3721 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3781 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3841 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3901 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3961 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4021 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4081 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4141 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4201 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4261 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4321 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4381 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4441 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4501 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4561 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4621 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4681 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4741 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4801 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4861 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4921 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4981 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5041 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5101 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5161 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5221 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5281 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5341 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5401 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5461 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5521 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5581 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5641 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5701 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5761 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5821 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5881 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5941 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6001 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6061 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6121 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6181 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6241 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6301 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6361 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6421 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6481 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6541 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6601 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6661 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6721 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6781 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6841 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6961 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7021 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7081 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7141 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7201 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7261 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7321 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7381 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7441 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7501 CAAAAATTTA ACGCGAATTT T
//