LOCUS       MR231255                7431 bp    DNA     circular  SYN 
DEFINITION  Pde10a (myc-DDK-tagged) - Mouse phosphodiesterase 10A (Pde10a), transcript variant 1
ACCESSION   NM_001290707
VERSION     NM_001290707.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3588..3679 
                     /label="Myc-DDK"  
     misc_feature    3709..4294 
                     /label="PolyA signal"  
     misc_feature    3766..3785 
                     /label="Restriction Enzyme"  
     misc_feature    4570..5242 
                     /label="ColE1"  
     misc_feature    5672..6466 
                     /label="Kan/Neo"  
     misc_feature    6501..6803 
                     /label="SV40 ori"  
     misc_feature    6889..7366 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3570..3576 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3570 
                     /label="Pde10a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAGAAG CGCAAAGCCC TCGAGGGTGG AGGAGAACCC CAGCTCCCAG 
     1081 AGGAGGAACC CACTGCCTGG TTTGGGGGCA GCAGCGAGGA GCCAGGCTGC CTCCCATTCG 
     1141 CACTTAAAGG TGGGAGCAAG GGCCCCGCTC TATTGGCACT TCGAAACCGC ACAGACTCTC 
     1201 GGGGGCAGAT GAGCAATGAC TCCACAGAAG GCACCGTGGG CTCCTGCAAC GCAACAGGTT 
     1261 TGACGGATGA AAAGGTGAAG GCCTATCTTT CTCTCCATCC CCAGGTATTA GATGAATTTG 
     1321 TTTCTGAAAG TGTTAGTGCA GAGACTGTGG AAAAGTGGCT GAAGAGGAAA ACCAACAAAG 
     1381 CAAAAGATGA ACCATCTCCC AAGGAAGTCA GCAGGTACCA GGATACGAAT ATGCAGGGAG 
     1441 TCGTGTACGA GCTGAACAGC TACATAGAGC AGCGCCTGGA CACGGGCGGG GACAACCACC 
     1501 TGCTCCTCTA TGAGCTCAGC AGCATCATCA GGATAGCCAC AAAAGCCGAC GGATTTGCAC 
     1561 TGTACTTCCT TGGAGAGTGC AATAATAGCC TGTGTGTGTT CATACCACCC GGGATGAAGG 
     1621 AAGGCCAACC CCGGCTCATC CCTGCAGGGC CCATCACCCA GGGTACCACC ATCTCTGCCT 
     1681 ACGTGGCCAA GTCTAGGAAG ACGTTGTTGG TAGAGGATAT CCTTGGGGAT GAGCGATTTC 
     1741 CTCGAGGTAC TGGCCTGGAA TCAGGAACCC GCATCCAGTC TGTTCTTTGC TTGCCCATTG 
     1801 TCACTGCCAT TGGAGACTTG ATTGGCATCC TTGAACTGTA CAGGCACTGG GGCAAAGAGG 
     1861 CCTTCTGCCT CAGCCATCAG GAGGTTGCAA CAGCCAATCT TGCTTGGGCT TCCGTAGCAA 
     1921 TACACCAGGT GCAGGTGTGT AGAGGTCTCG CCAAACAGAC CGAACTGAAT GACTTCCTAC 
     1981 TCGACGTATC AAAGACATAC TTTGATAACA TAGTTGCCAT AGACTCTCTA CTTGAACACA 
     2041 TCATGATATA TGCAAAAAAT CTAGTGAACG CCGACCGCTG CGCGCTCTTC CAGGTGGACC 
     2101 ACAAGAACAA GGAGCTGTAC TCGGACCTGT TTGACATTGG GGAGGAGAAG GAGGGGAAGC 
     2161 CCATCTTCAA GAAGACCAAG GAGATCAGAT TTTCCATTGA GAAAGGGATT GCTGGTCAAG 
     2221 TGGCAAGAAC AGGCGAAGTC TTGAACATTC CCGATGCCTA CGCGGACCCT CGCTTTAACA 
     2281 GGGAGGTGGA CCTGTACACA GGCTACACCA CGAGGAACAT TCTGTGTATG CCCATAGTGA 
     2341 GCCGAGGCAG CGTGATTGGC GTGGTGCAGA TGGTGAACAA GATCAGCGGT AGCGCCTTCT 
     2401 CCAAGACAGA CGAGAACAAC TTCAAGATGT TTGCTGTCTT CTGCGCACTG GCCTTGCACT 
     2461 GTGCTAACAT GTACCACAGG ATCCGCCACT CAGAATGCAT CTACAGGGTT ACCATGGAGA 
     2521 AGCTTTCCTA CCACAGCATC TGCACCTCCG AGGAGTGGCA AGGCCTCATG CGCTTCAACC 
     2581 TACCAGCACG CATCTGCCGG GACATCGAGC TATTCCACTT TGACATTGGT CCTTTCGAGA 
     2641 ACATGTGGCC TGGGATCTTT GTCTACATGA TCCATCGGTC TTGTGGGACA TCCTGTTTTG 
     2701 AACTTGAAAA ATTGTGCCGT TTTATCATGT CTGTGAAGAA GAACTATCGG CGGGTTCCTT 
     2761 ACCACAACTG GAAGCATGCA GTCACGGTGG CACACTGCAT GTATGCCATA CTTCAAAACA 
     2821 ACAATGGCCT CTTCACAGAC CTCGAGCGCA AAGGCCTGCT AATTGCGTGT CTGTGCCATG 
     2881 ACCTGGACCA CAGGGGCTTC AGTAACAGCT ACCTGCAGAA GTTCGACCAC CCCCTGGCGG 
     2941 CGCTGTACTC CACCTCCACC ATGGAGCAAC ACCACTTCTC CCAGACGGTG TCCATCCTTC 
     3001 AGCTGGAAGG GCACAATATC TTCTCCACCC TGAGCTCCAG CGAGTACGAG CAGGTGCTGG 
     3061 AGATCATCCG CAAAGCCATC ATCGCCACCG ACCTCGCCCT ATACTTTGGG AACAGGAAGC 
     3121 AGTTGGAGGA GATGTACCAG ACAGGGTCGC TGAACCTCCA CAACCAGTCC CATCGAGACC 
     3181 GTGTCATCGG CTTGATGATG ACTGCCTGTG ATCTTTGCTC TGTGACCAAA CTATGGCCAG 
     3241 TTACAAAATT GACAGCGAAT GATATATATG CAGAATTCTG GGCTGAGGGT GATGAGATGA 
     3301 AGAAGCTGGG CATACAGCCC ATTCCTATGA TGGACAGAGA CAAGCGAGAT GAAGTCCCTC 
     3361 AAGGGCAGCT CGGATTCTAC AATGCTGTGG CCATTCCCTG CTATACCACC TTGACGCAGA 
     3421 TCCTCCCACC CACAGAGCCT CTGCTGAAGG CCTGCAGGGA TAACCTCAAT CAGTGGGAGA 
     3481 AGGTAATTCG CGGGGAAGAG ACAGCAATGT GGATTTCAGG CCCAGGCCCG GCGCCTAGCA 
     3541 AGAGCACACC TGAGAAGCTG AACGTGAAGG TTGAAGACAC GCGTACGCGG CCGCTCGAGC 
     3601 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3661 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3721 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3781 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3841 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3901 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3961 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4021 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4081 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4141 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4201 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4261 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4321 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4381 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4441 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4501 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4561 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4621 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4681 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4741 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4801 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4861 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4921 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4981 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5041 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5101 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5161 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5221 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5281 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5341 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5401 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5461 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5521 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5581 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5641 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5701 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5761 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5821 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5881 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5941 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6001 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6061 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6121 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6181 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6241 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6301 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6361 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6421 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6481 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6541 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6601 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6661 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6721 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6781 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6841 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6901 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6961 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7021 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7081 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7141 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7201 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7261 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7321 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7381 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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