LOCUS       MR231237                7407 bp    DNA     circular  SYN 
DEFINITION  Atg9a (myc-DDK-tagged) - Mouse autophagy related 9A (Atg9a), transcript variant 1
ACCESSION   NM_001288612
VERSION     NM_001288612.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3562..3653 
                     /label="Myc-DDK"  
     misc_feature    3683..4268 
                     /label="PolyA signal"  
     misc_feature    3740..3759 
                     /label="Restriction Enzyme"  
     misc_feature    4544..5216 
                     /label="ColE1"  
     misc_feature    5646..6440 
                     /label="Kan/Neo"  
     misc_feature    6475..6777 
                     /label="SV40 ori"  
     misc_feature    6863..7340 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3537..3544 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3537 
                     /label="Atg9a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAGTTT GACACTGAAT ACCAGCGCCT AGAGGCCTCC TATAGCGATT 
     1081 CGCCCCCCGG GGAAGAGGAC CTGTTGGTGC ATGTGGCCGA GGGGAGCAAA TCACCTTGGC 
     1141 ACCACATTGA AAACCTTGAC CTCTTCTTCT CTCGAGTTTA TAATCTCCAC CAGAAGAATG 
     1201 GCTTCACATG TATGCTCATT GGAGAGATGT TTGAACTCAT GCAGTTCCTC TTTGTGGTTG 
     1261 CCTTCACCAC CTTCCTGGTT AGCTGTGTGG ACTATGACAT CCTCTTTGCC AACAAGATGG 
     1321 TGAACCACAG TCTCCATCCT ACTGAGCCTG TCAAGGTCAC TCTGCCAGAT GCCTTTTTGC 
     1381 CTGCCCAAGT CTGTAGTGCC AGGATTCAGG AAAATGGCTC TCTTATCACC ATCCTGGTCA 
     1441 TTGCTGGTGT CTTCTGGATC CACCGGCTTA TCAAGTTTAT CTATAACATT TGCTGCTATT 
     1501 GGGAGATCCA CTCCTTCTAC CTACATGCTC TTCGGATCCC AATGTCTGCC CTTCCATACT 
     1561 GCACATGGCA GGAAGTTCAG GCCCGGATTG TGCAGACGCA GAAAGAGCAT CAGATCTGCA 
     1621 TCCACAAGCG TGAGCTGACA GAGTTGGACA TCTACCATCG CATCCTACGT TTCCAGAACT 
     1681 ACATGGTGGC CCTGGTGAAC AAATCCCTCC TGCCTCTGCG CTTCCGTCTG CCCGGCCTCG 
     1741 GAGAGGTTGT CTTCTTCACC CGTGGCCTCA AGTACAACTT TGAGCTCATC CTCTTCTGGG 
     1801 GACCCGGCTC TCTGTTTCTC AATGAGTGGA GCCTCAAGGC CGAGTACAAA CGTGGAGGGC 
     1861 AACGGCTAGA GCTGGCCCAG CGTCTCAGCA ACCGCATCTT GTGGATTGGC ATCGCCAACT 
     1921 TCCTGCTGTG TCCCCTCATC CTCATCTGGC AGATCCTCTA TGCCTTCTTC AGCTATGCCG 
     1981 AGGTGCTGAA GAGAGAGCCG GGGGCCCTGG GAGCGCGTTG CTGGTCACTC TATGGCCGTT 
     2041 GTTACCTCCG CCACTTCAAT GAGCTGGAGC ATGAGCTGCA GTCCCGCCTC AACCGAGGCT 
     2101 ACAAGCCCGC CTCCAAGTAC ATGAATTGCT TCTTGTCACC GCTGCTGACT CTGCTGGCCA 
     2161 AGAATGGTGC CTTCTTCGCT GGCTCTATCC TGGCTGTGCT TATTGCCCTC ACCATCTATG 
     2221 ATGAAGATGT GTTAGCTGTG GAACACGTCC TCACCACGGT CACCCTCCTG GGAGTCACGG 
     2281 TGACTGTGTG CAGGTCCTTC ATCCCAGACC AGCACATGGT GTTCTGCCCC GAGCAGCTGC 
     2341 TCCGAGTGAT TCTTGCACAC ATCCACTACA TGCCTGACCA CTGGCAGGGT AATGCCCACC 
     2401 GCTCGCAGAC CCGGGACGAG TTTGCCCAGC TCTTCCAGTA CAAGGCAGTG TTCATCTTGG 
     2461 AGGAGTTGCT GAGTCCCATC GTCACACCCC TCATTCTCAT CTTCTGCCTC CGCCCTCGGG 
     2521 CCCTGGAGAT CATAGACTTC TTCCGCAACT TTACGGTCGA GGTCGTGGGT GTGGGAGACA 
     2581 CCTGCTCCTT TGCTCAGATG GACGTTCGCC AGCATGGCCA TCCTCAGTGG CTGTCTGGAG 
     2641 GGCAGACAGA GGCCTCAGTG TACCAGCAAG CCGAGGACGG GAAGACCGAG TTGTCGCTCA 
     2701 TGCACTTTGC CATCACCAAT CCCGGCTGGC AGCCCCCTCG TGAGAGCACA GCTTTCCTGG 
     2761 GCTTCCTCAA GGAGCAGGTG CAGCGAGATG GAGCAGCTGC TGGCCTGGCC CAGGGTGGCC 
     2821 TGCTCCCCGA GAATGCCCTC TTCACATCCA TCCAGTCCTT ACAGTCTGAG TCCGAGCCAC 
     2881 TGAGCCTTAT TGCAAATGTG GTAGCAGGCT CATCCTGCCG AGGACCCTCA CTGTCCAGAG 
     2941 ACCTGCAGGG CTCCAGGCAC AGGGCTGATG TTGCTTCTGC CCTTCGATCC TTCTCCCCTC 
     3001 TGCAGCCTGG AGCGGCCCCT CAAGGCCGGG TTCCCAGTAC CATGACAGGC TCTGGAGTGG 
     3061 ATGCCAGGAC AGCCAGCTCT GGGAGTAGCG TGTGGGAAGG ACAGCTGCAG AGCCTGGTGC 
     3121 TGTCCGAATA CGCGTCCACC GAGATGAGCC TGCACGCCCT GTACATGCAC CAGCTCCACA 
     3181 AGCAGCAGAC CCAGGCTGAG CCCGAGCGGC ATGTGTGGCA CCGCCGGGAG AGTGATGAGA 
     3241 GTGGAGAGAG TGCCCCTGAA GAGGGGGGAG AGGGTGCCCG GGCCCCCCAA CCCATCCCCC 
     3301 GCTCGGCCAG CTATCCCTGT GCTACACCCC GGCCTGGAGC ACCTGAGACC ACCGCCCTGC 
     3361 ATGGGGGCTT CCAGAGGCGC TACGGGGGCA TCACAGATCC TGGCACAGTG CCCCGTGGCC 
     3421 CCTCTCACTT CTCCAGGCTG CCCCTGGGAG GATGGGCAGA AGATGGCCAG CCAGCATCAA 
     3481 GGCACCCAGA GCCGGTGCCA GAGGAGGGCT CAGAGGATGA ACTCCCCCCT CAAGTGCACA 
     3541 AGGTAAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3601 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3661 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3721 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3781 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3841 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3901 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3961 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4021 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4081 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4141 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4201 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4261 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4321 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4381 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4441 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4501 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4561 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4621 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4681 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4741 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4801 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4861 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4921 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4981 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5041 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5101 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5161 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5221 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5281 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5341 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5401 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5461 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5521 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5581 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5641 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5701 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5761 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5821 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5881 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5941 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6001 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6061 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6121 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6181 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6241 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6301 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6361 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6421 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6481 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6541 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6601 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6661 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6721 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6781 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6841 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6901 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6961 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7021 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7081 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7141 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7201 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7261 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7321 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7381 ATTTAACAAA AATTTAACGC GAATTTT
//