LOCUS       MR231224                7371 bp    DNA     circular  SYN 
DEFINITION  Cdc27 (myc-DDK-tagged) - Mouse cell division cycle 27 (Cdc27), transcript variant 1
ACCESSION   NM_001285988
VERSION     NM_001285988.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3528..3619 
                     /label="Myc-DDK"  
     misc_feature    3649..4234 
                     /label="PolyA signal"  
     misc_feature    3706..3725 
                     /label="Restriction Enzyme"  
     misc_feature    4510..5182 
                     /label="ColE1"  
     misc_feature    5612..6406 
                     /label="Kan/Neo"  
     misc_feature    6441..6743 
                     /label="SV40 ori"  
     misc_feature    6829..7306 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3510..3516 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3510 
                     /label="Cdc27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGTGCTG CAGGAACCTG TCCAGGCTGC TATATGGCAA GCGCTAAACC 
     1081 ACTATGCTTA CCGAGATGCA GTTTTCCTCG CAGAACGACT ATATGCAGAA GTACATTCAG 
     1141 AAGAAGCCTT GTTTTTACTG GCAACCTGTT ACTACCGCTC AGGAAAGGCT TATAAAGCAT 
     1201 ATAGACTCTT GAAAGGACAC AGTTGTACCA CCCCACAGTG TAAATACCTG CTTGCAAAAT 
     1261 GTTGTGTTGA CCTCAGCAAG CTTGCAGAAG GGGAACAGAT CTTATCTGGT GGAGTGTTTA 
     1321 ATAAGCAGAA AAGCCATGAC GACCTTGTCA CTGAGTTTGG AGATTCAGCT TGCTTCACTC 
     1381 TTTCCTTGTT GGGACATGTG TATTGCAAGA CAGATCGGCT TGCCAAAGGG TCAGAATGTT 
     1441 ACCAAAAGAG CCTTAGTTTA AATCCTTTCC TCTGGTCTCC CTTTGAATCG TTATGTGAAA 
     1501 TAGGTGAGAA GCCAGATCCT GACCAAACAT TTAAATTAAC ATCTCTACAG AATTTTAGCA 
     1561 GTTGTCTTCC CAACACTTGT ACAACTCTAG TATCTAATCA CAGTTTATCT CACAGACAGC 
     1621 CTGAGACAGT CCTTACAGAA ACACCCCAAG ACACAATTGA ATTAAACAGA CTGAATTTAG 
     1681 AATCTTCCAA TTCAAAGTAC TCCTTGAATA CAGATTCCTC CGTGTCTTAC ATTGATTCAA 
     1741 CTGTAATTTC ACCAGATAAC GTCCCCCTTG GACCTGGTAC TGCCATATTA TCTAAACAGG 
     1801 TTCAAAATAA ACCAAAAACT GGTCGAAGTT TATTAGGAGG ACCAACTGCT CTTAGCCCAT 
     1861 TAACCCCAAG TTTTGGGATT TTGCCATTAG AAACCCCAAG TCCTGGAGAT GGATCCTATT 
     1921 TACAAAACTA CACTAATACA CCTTCTGTAA TTGATGTGGC ACCCACCGGA GCACCTACAA 
     1981 AAAAGACTTT TTGTGTTTTA CAGTCTGTTG CAAGAATGGG CCAAACTGGA ACAAAGTCTG 
     2041 TCTTCTCACA GAGTGGAAAT AGTCGAGAGG TCACACCAGT ACTTGTTGCA CAAACACAAA 
     2101 GTTCTGGCCC ACAAACAAGT ACAACACCTC AGGTATTGAG CCCCACTATC ACATCTCCCC 
     2161 CAAACGCATT GCCTCGGAGA AGTTCCCGCC TTTTCACTAG TGACAGTTCT ACAACCAAGG 
     2221 AGAATAGCAA AAAGTTAAAA ATGAAGTTTC CACCTAAAAT CCCTAACAGA AAAACAAAAA 
     2281 GTAAAACTAA TAAAGGAGGA CTAACTCAGC CCAGCATAAA CGATAGTCTG GAAATTACAA 
     2341 AACTGGACTC CTCTATCATT TCAGAAGGGA AAATAACCAC AGTCACACCT CAGATCCAGG 
     2401 CATTTAACCT ACAAAAGGCA GCAGCAGGTT TGATGAGCCT TCTTCGTGAA ATGGGAAAAG 
     2461 GTTATTTAGC TTTGTGTTCA TACAACTGCA AAGAAGCTAT CAATATTTTG AGCCATCTAC 
     2521 CTTCTCATCA CTACAGTACT GGTTGGGTCC TATGCCAGAT TGGACGGGCT TATTTTGAAC 
     2581 TTTCAGAATA CATGCAGGCT GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAGTTTCA 
     2641 GAGTTGAAGG AATGGAGATC TACTCTACAA CACTCTGGCA TCTTCAGAAA GACGTTGCTC 
     2701 TTTCAGTTCT TTCAAAAGAT TTAACAGACA TGGATAAGAA TTCACCAGAG GCCTGGTGTG 
     2761 CTGCAGGGAA TTGTTTCAGT CTACAACGAG AGCATGATAT AGCAATTAAA TTCTTCCAAA 
     2821 GAGCTATCCA GGTCGATCCA AATTACGCTT ACGCCTATAC TCTATTAGGA CATGAGTTTG 
     2881 TGTTAACTGA AGAACTAGAT AAAGCATTAG CATGTTTTCG AAATGCTATA AGAGTAAATC 
     2941 CCAGACATTA CAATGCATGG TATGGTTTAG GAATGATTTA TTACAAGCAA GAGAAGTTCA 
     3001 GCCTCGCAGA AATGCATTTC CAGAAAGCAC TTGATATCAA CCCTCAGAGT TCAGTTTTAC 
     3061 TTTGCCACAT TGGAGTAGTT CAGCATGCAC TAAAGAAGTC TGAGAAGGCT TTGGATACCC 
     3121 TAAACAAAGC CATTGTTATC GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCGG 
     3181 TTTTATTTGC AAATGAAAAA TACAAGTCTG CTTTACAAGA ACTCGAGGAA CTGAAGCAGA 
     3241 TTGTCCCCAA AGAGTCCCTC GTTTACTTCC TAATAGGAAA GGTTTACAAG AAGTTGGGTC 
     3301 AAACTCATCT CGCCTTGATG AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA 
     3361 ATAATCAGAT TAAAGAGGCA ATTGACAAGC GCTACCTTCC AGATGATGAG GAGCCAATAA 
     3421 CCCAGGAGGA ACAGATCATG GGGACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCAG 
     3481 ATGACACACA ACTTCATGCG GCGGAAAGTG ACGAATTTAC GCGTACGCGG CCGCTCGAGC 
     3541 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3601 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3661 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3721 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3781 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3841 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3901 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3961 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4021 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4081 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4141 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4201 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4261 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4321 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4381 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4441 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4501 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4561 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4621 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4681 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4741 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4801 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4861 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4921 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4981 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5041 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5101 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5161 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5221 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5281 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5341 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5401 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5461 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5521 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5581 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5641 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5701 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5761 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5821 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5881 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5941 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6001 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6061 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6121 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6181 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6241 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6301 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6361 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6421 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6481 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6541 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6601 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6661 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6721 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6781 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6841 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6901 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6961 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7021 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7081 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7141 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7201 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7261 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7321 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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