LOCUS       MR231207                7338 bp    DNA     circular  SYN 
DEFINITION  Nell2 (myc-DDK-tagged) - Mouse NEL-like 2 (Nell2)
ACCESSION   NM_001289653
VERSION     NM_001289653.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3495..3586 
                     /label="Myc-DDK"  
     misc_feature    3616..4201 
                     /label="PolyA signal"  
     misc_feature    3673..3692 
                     /label="Restriction Enzyme"  
     misc_feature    4477..5149 
                     /label="ColE1"  
     misc_feature    5579..6373 
                     /label="Kan/Neo"  
     misc_feature    6408..6710 
                     /label="SV40 ori"  
     misc_feature    6796..7273 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3477..3483 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3477 
                     /label="Nell2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACGCCATG GAATCCCGGG TGTTACTGAG AACGTTCTGC GTGATCCTCG 
     1081 GGCTTGGAGC GGTTTGGGGG CTTGGTGTGG ACCCCTCCCT ACAGATTGAC GTCTTAACAG 
     1141 AGTTAGAACT TGGGGAGTCT ACAGATGGAG TGCGCCAAGT CCCGGGACTG CATAATGGGA 
     1201 CGAAAGCCTT CCTCTTCCAA GAGTCCCCCA GAAGCATAAA GGCATCCACT GCTACAGCTG 
     1261 AGCGGTTTTT CCAGAAGCTG AGAAATAAAC ACGAGTTCAC AATCTTGGTG ACCCTGAAAC 
     1321 AGATCCACTT AAATTCGGGA GTTATCCTCT CCATCCACCA CTTGGATCAC AGGTACCTGG 
     1381 AACTGGAAAG CAGTGGCCAT CGGAATGAGA TCAGACTCCA CTACCGCTCT GGCACTCACC 
     1441 GTCCCCACAC GGAAGTGTTT CCTTATATTT TGGCTGATGC CAAGTGGCAC AAGCTCTCCT 
     1501 TAGCCTTCAG TGCCTCTCAC TTAATTTTAC ACATCGACTG CAATAAGATC TATGAACGAG 
     1561 TGGTGGAAAT GCCTTCAACA GACTTGCCTC TGGGCACCAC ATTTTGGTTG GGACAGAGAA 
     1621 ATAATGCACA TGGCTATTTT AAGGGAATAA TGCAGGATGT GCACGTTCTT GTCATGCCTC 
     1681 AGGGCTTCAT TGCTCAGTGC CCGGACCTTA ATCGAACCTG TCCAACATGC AACGACTTCC 
     1741 ATGGGCTTGT GCAGAAAATC ATGGAGCTGC AGGACATTTT ATCAAAGACG TCAGCCAAGT 
     1801 TGTCCAGAGC TGAACAAAGA ATGAACAGGC TGGATCAGTG CTACTGTGAG CGGACATGCA 
     1861 CTGTGAAGGG AACCACCTAC CGAGAGTCTG AGTCCTGGAC AGACGGCTGT AAGAACTGCA 
     1921 CATGCTTGAA CGGGACCATC CAGTGCGAGA CTCTGGTCTG CCCTGCTCCT GACTGCCCTC 
     1981 CTAAATCGGC CCCTGCGTAT GTGGATGGCA AGTGCTGTAA GGAGTGCAAA TCAACCTGCC 
     2041 AGTTCCAGGG ACGGAGCTAC TTTGAGGGAG AAAGGAACAC GGTATACTCA TCTTCTGGAA 
     2101 TGTGTGTCTT ATATGAATGC AAGGATCAGA CCATGAAGCT TGTTGAGAAC ATTGGCTGCC 
     2161 CACCCTTAGA TTGTCCCGAG TCTCATCAGA TTGCCTTGTC TCACAGCTGC TGCAAGGTTT 
     2221 GTAAAGGTTA TGACTTCTGT TCTGAGAAGC ATACCTGCAT GGAGAACTCG GTCTGCAGGA 
     2281 ACCTGAACGA CAGGGCTGTG TGCAGCTGCA GGGATGGTTT TCGGGCTCTC CGAGAGGACA 
     2341 ACGCCTACTG TGAAGACATT GACGAGTGTG CAGAAGGGCG CCATTACTGC CGTGAGAACA 
     2401 CCATGTGTGT GAATACACCT GGTTCTTTCA TGTGTATCTG CAAAACTGGG TACATCAGGA 
     2461 TCGACGATTA CTCATGTACA GAACATGATG AGTGTCTCAC AAACCAGCAC AATTGTGATG 
     2521 AAAACGCTTT GTGCTTTAAC ACTGTTGGAG GACACAACTG TGTCTGCAAG CCTGGCTACA 
     2581 CCGGGAATGG AACCACGTGC AAAGCTTTCT GCAAAGATGG CTGTAGAAAC GGAGGAGCGT 
     2641 GCATTGCTGC CAATGTGTGT GCCTGCCCAC AAGGCTTCAC GGGACCCAGC TGTGAGACAG 
     2701 ACATTGACGA GTGCTCTGAG GGCTTTGTTC AGTGTGACAG CCGTGCCAAC TGCATCAACC 
     2761 TGCCTGGGTG GTATCACTGT GAGTGCAGAG ACGGCTACCA TGACAATGGG ATGTTTGCGC 
     2821 CAGGCGGAGA ATCCTGTGAA GATATTGACG AATGCGGGAC TGGGAGGCAC AGCTGCACCA 
     2881 ACGACACCAT TTGCTTCAAC TTGGACGGGG GATACGATTG CCGGTGTCCC CATGGGAAGA 
     2941 ACTGCACTGG GGACTGCGTG CACGAGGGGA AAGTGAAGCA CACCGGCCAG ATCTGGGTGC 
     3001 TGGAAAACGA CAGGTGCTCC GTGTGTTCCT GCCAGACTGG GTTTGTCATG TGTCGACGGA 
     3061 TGGTCTGCGA CTGCGAAAAC CCCACAGTTG ACCTTTCCTG CTGCCCTGAG TGTGACCCAA 
     3121 GGCTGAGCAG TCAGTGCCTG CATCAAAACG GGGAAACCGT GTACAACAGC GGCGACACCT 
     3181 GGGTCCAGGA TTGCCGTCAG TGCCGCTGCT TGCAAGGAGA AGTTGACTGT TGGCCCCTGG 
     3241 CTTGCCCAGA GGTAGAATGT GAATTTAGCG TCCTTCCTGA GAACGAGTGC TGCCCACGCT 
     3301 GTGTCACCGA TCCTTGTCAG GCCGACACCA TCCGCAATGA CATCACCAAA ACCTGCCTGG 
     3361 ACGAGATGAA CGTGGTTCGC TTCACCGGGT CTTCCTGGAT CAAGCACGGC ACGGAGTGTA 
     3421 CCCTCTGCCA GTGCAAGAAT GGCCATTTGT GCTGCTCAGT GGATCCACAG TGCCTTCAGG 
     3481 AGCTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3541 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3601 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3661 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3721 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3781 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3841 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3901 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3961 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4021 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4081 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4141 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4201 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4261 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4321 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4381 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4441 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4501 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4561 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4621 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4681 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4741 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4801 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4861 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4921 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4981 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5041 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5101 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5161 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5221 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5281 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5341 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5401 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5461 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5521 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5581 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5641 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5701 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5761 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5821 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5881 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5941 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6001 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6061 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6121 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6181 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6241 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6301 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6361 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6421 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6481 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6541 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6601 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6661 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6721 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6781 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6841 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6901 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6961 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7021 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7081 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7141 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7201 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7261 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7321 AAATTTAACG CGAATTTT
//