LOCUS       MR231200                7329 bp    DNA     circular  SYN 
DEFINITION  Pi4kb (myc-DDK-tagged) - Mouse phosphatidylinositol 4-kinase, catalytic, beta polypeptide (Pi4kb), transcript variant 1
ACCESSION   NM_001293715
VERSION     NM_001293715.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3486..3577 
                     /label="Myc-DDK"  
     misc_feature    3607..4192 
                     /label="PolyA signal"  
     misc_feature    3664..3683 
                     /label="Restriction Enzyme"  
     misc_feature    4468..5140 
                     /label="ColE1"  
     misc_feature    5570..6364 
                     /label="Kan/Neo"  
     misc_feature    6399..6701 
                     /label="SV40 ori"  
     misc_feature    6787..7264 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3468..3474 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3468 
                     /label="Pi4kb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGACATG GTAGTGGAGC CTGCCACCCT GAAGCCAACT TCTGAGCCTA 
     1081 CTCCTAGCCC ATCAGGGAAT AATGGGGGCT CCCTACTAAG CGTCATCACG GAGGGGGTCG 
     1141 GGGAACTGTC AGTGATTGAC CCTGAGGTGG CCCAGAAGGC CTGCCAGGAG GTACTGGAGA 
     1201 AAGTCAAGCT TTTGCATGGA GGTGTAGCCA TCTCTAGCAA AGGCACCCCG CTGGAGTTGG 
     1261 TTAATGGGGA TGGTGTGGAC AATGAAATCC GTTGCCTAGA TGATCCACCT GCCCAGATCA 
     1321 GGGAGGAAGA AGATGAGATG GGGGCTGGTG TGGCCTCTGG CACAGCCAAA GGAGCAAGAC 
     1381 GACGACGACA GAACAACTCA GCCAAACAGT CTTGGCTCCT GAGGCTGTTT GAGTCAAAAC 
     1441 TATTTGACAT CTCTATGGCT ATTTCATACT TGTATAACTC CAAGGAGCCT GGAGTGCAAG 
     1501 CCTACATTGG CAACCGGCTC TTCTACTTTC GCAATGAGGA TGTGGACTTC TATTTGCCCC 
     1561 AGTTGCTTAA CATGTATATC CACATGGATG AGGATGTGGG CGATGCCATT AAGCCCTACA 
     1621 TAGTCCACCG CTGTCGCCAG AGCATCAACT TTTCCCTCCA GTGTGCCCTG TTGCTTGGGG 
     1681 CCTACTCTTC AGACATGCAC ATTTCCACTC AGCGACACTC GCGAGGGACC AAGTTACGGA 
     1741 AGCTAATCCT CTCAGATGAG CTGAAGCCAG CTCACCGAAA GAGGGAGCTG CCGACATTAA 
     1801 GCCCAGCCCC TGACACAGGG CTGTCTCCCT CTAAAAGGAC TCACCAGCGT TCTAAGTCAG 
     1861 ATGCTACGGC CAGCATAAGT CTCAGCAGCA ACCTGAAACG AACAGCCAGC AACCCTAAAG 
     1921 TGGAGAATGA GGATGAGGAG CTCTCCTCCA GCACCGAGAG TATTGATAAT TCATTCAGTT 
     1981 CCCCTGTCCG GCTGGCCCCT GAGCGAGAAT TCATCAAGTC TTTGATGGCA ATCGGCAAAC 
     2041 GGCTGGCCAC GCTCCCCACT AAAGAGCAAA AAACACAAAG GCTGATCTCA GAGCTTTCCC 
     2101 TGCTCAACCA TAAGCTCCCT GCCCGAGTCT GGCTGCCCAC GGCTGGCTTT GACCACCACG 
     2161 TGGTCCGTGT GCCCCACACA CAAGCTGTTG TTCTCAATTC CAAGGATAAG GCTCCCTACC 
     2221 TGATCTACGT GGAAGTTCTC GAATGTGAAA ACTTTGACAC AACTAGTGTT CCTGCCCGGA 
     2281 TTCCTGAAAA CCGAATTCGG AGTACACGGT CTGTAGAGAA CCTGCCTGAA TGTGGTATCA 
     2341 CTCATGAGCA GCGAGCTGGC AGCTTCAGCA CTGTGCCCAA TTATGACAAT GATGATGAAG 
     2401 CCTGGTCAGT GGATGATATA GGCGAGCTGC AAGTGGAGCT CCCTGAAGTG CACACCAACA 
     2461 GCTGTGACAA CATCTCCCAG TTCTCGGTGG ACAGCATCAC CAGCCAGGAG AGCAAGGAGC 
     2521 CTGTGTTCAT TGCAGCAGGG GACATCCGAC GGCGCCTTTC AGAACAGCTG GCTCATACTC 
     2581 CTACAGCCTT CAAACGAGAC CCTGAAGACC CTTCTGCAGT TGCCCTCAAA GAGCCCTGGC 
     2641 AGGAGAAAGT GCGGAGGATC AGAGAAGGTT CCCCGTATGG CCATCTTCCC AATTGGCGAC 
     2701 TCCTTTCAGT CATTGTCAAG TGTGGAGATG ACCTTCGCCA GGAGCTGCTG GCTTTCCAGG 
     2761 TGTTGAAACA ACTGCAGTCC ATTTGGGAAC AGGAGCGAGT GCCTCTTTGG ATCAAGCCAT 
     2821 ATAAGATTCT TGTGATTTCA GCTGACAGTG GCATGATTGA ACCAGTAGTC AACGCTGTGT 
     2881 CCATCCACCA GGTGAAGAAA CAGTCACAGC TCTCCTTGCT CGATTACTTC CTACAGGAAC 
     2941 ATGGCAGTTA TACCACTGAG GCATTCCTCA GTGCCCAGCG CAATTTTGTG CAAAGCTGTG 
     3001 CTGGCTACTG CTTGGTCTGC TACCTATTGC AAGTCAAGGA CAGGCACAAC GGGAACATCC 
     3061 TTCTGGACGC AGAAGGTCAC ATCATCCACA TCGACTTTGG CTTCATCCTT TCCAGCTCAC 
     3121 CCCGAAACCT GGGCTTCGAG ACATCAGCCT TTAAGCTGAC CACAGAATTT GTGGATGTAA 
     3181 TGGGTGGCCT GAACGGTGAT ATGTTCAACT ACTACAAGAT GCTCATGCTG CAAGGGCTGA 
     3241 TTGCTGCTCG GAAGCACATG GACAAGGTGG TACAGATTGT GGAGATCATG CAGCAAGGTT 
     3301 CTCAGCTTCC TTGCTTCCAT GGCTCCAGCA CCATTCGCAA CCTCAAAGAG AGGTTCCACA 
     3361 TGAGCATGAC TGAGGAGCAG CTGCAGCTGC TGGTGGAGCA GATGGTGGAC GGCAGCATGA 
     3421 GGTCCATCAC CACCAAACTC TACGATGGCT TCCAGTACCT CACCAATGGC ATCATGACGC 
     3481 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3541 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3601 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3661 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3721 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3781 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3841 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3901 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3961 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4021 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4081 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4141 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4201 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4261 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4321 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4381 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4441 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4501 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4561 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4621 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4681 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4741 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4801 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4861 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4921 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4981 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5041 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5101 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5161 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5221 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5281 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5341 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5401 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5461 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5521 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5581 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5641 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5701 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5761 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5821 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5881 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5941 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6001 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6061 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6121 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6181 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6241 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6301 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6361 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6421 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6481 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6541 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6601 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6721 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6781 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6841 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6901 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6961 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7021 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7081 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7141 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7201 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7261 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7321 GCGAATTTT
//