LOCUS       MR231092                7170 bp    DNA     circular  SYN 
DEFINITION  Cux1 (myc-DDK-tagged) - Mouse cut-like homeobox 1 (Cux1), transcript variant 2
ACCESSION   NM_001291240
VERSION     NM_001291240.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3327..3418 
                     /label="Myc-DDK"  
     misc_feature    3448..4033 
                     /label="PolyA signal"  
     misc_feature    3505..3524 
                     /label="Restriction Enzyme"  
     misc_feature    4309..4981 
                     /label="ColE1"  
     misc_feature    5411..6205 
                     /label="Kan/Neo"  
     misc_feature    6240..6542 
                     /label="SV40 ori"  
     misc_feature    6628..7105 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3309..3315 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3309 
                     /label="Cux1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCG TCAGGTCCGC TCCGAGCTGC CTTCTCCAGC CCGGTTCTCG 
     1081 AAAGCAAAGC CCCGGGGGCC CCGCTGGACT GTAAGGAGAC TGATTGTGGT TCCTCGTCGC 
     1141 CTCGAGGGGA TGGTATCGGG GCTTCCCAAT CCGCGGCGGA GCTGCCTGCG TGCCCAGAGG 
     1201 ATGCTATTCT GCCTGCCACC CCTCATCGGA ACCAGCTGCT CGCCCTCGGG GTCCTCCCTG 
     1261 CTAGTGCTAG CTCCGCGTTC GCGGCGCCTG GACAGCCCCA GGATTCTGCC AGGTGGATGT 
     1321 TGTGCGTAGC CGGAGCCAAG TTGAAGAGAG AACTTGATGC CACCGCAACA GTATTGGCAA 
     1381 ACAGGCAAGA TGAGAGCGAA CAGTCCAGAA AGCGGCTCAT TGAGCAGAGC CGAGAATTCA 
     1441 AGAAGAACAC TCCAGAGGAT TTACGCAAGC AGGTAGCACC ACTGCTAAAG AGCTTCCAAG 
     1501 GGGAGATTGA TGCACTGAGT AAAAGAAGCA AAGAAGCAGA GGCAGCCTTC TTGACTGTGT 
     1561 ACAAGAGACT AATTGATGTT CCAGATCCGG TACCAGCCCT GGACGTCGGG CAACAGCTGG 
     1621 AAATAAAAGT GCAGCGTCTA CACGACATTG AAACAGAGAA CCAGAAACTT AGGGAAACAC 
     1681 TAGAAGAGTA CAACAAGGAG TTTGCTGAAG TGAAAAATCA AGAGGTTACG ATAAAAGCAC 
     1741 TTAAGGAGAA AATCCGAGAA TACGAGCAGA CCCTGAAGAG TCAGGCCGAG ACCATTGCTC 
     1801 TGGAGAAAGA GCAGAAGCTA CAAAATGATT TTGCAGAGAA GGAGAGAAAG CTGCAAGAGA 
     1861 CACAGATGTC CACCACCTCA AAACTGGAGG AAGCTGAGCA CAAACTCCAG ACTCTGCAAA 
     1921 CAGCCCTGGA AAAAACTCGA ACAGAATTAT TTGACCTGAA AACCAAATAT GATGAAGAAA 
     1981 CTACTGCAAA GGCCGATGAG ATCGAGATGA TCATGACCGA CCTTGAACGA GCCAACCAGA 
     2041 GGGCAGAGGT GGCACAGAGA GAAGCAGAGA CTTTAAGGGA ACAGCTCTCA TCGGCCAACC 
     2101 ACTCTCTCCA ACTGGCCTCG CAGATCCAGA AGGCTCCAGA TGTGGAGCAG GCCATAGAGG 
     2161 TGCTGACCCG ATCCAGCCTA GAAGTAGAGT TGGCTGCCAA AGAGCGGGAG ATCGCCCAGC 
     2221 TGGTGGAAGA TGTGCAGCGA CTCCAGGCCA GCCTCACCAA GCTACGTGAG AATTCCGCCA 
     2281 GCCAGATCTC ACAGCTGGAG CAGCAACTGA ATGCCAAGAA TAGCACACTC AAACAACTGG 
     2341 AAGAAAAACT CAAAGGCCAG GCTGACTATG AAGAAGTGAA GAAAGAGCTG AACACCCTGA 
     2401 AGTCCATGGA GTTTGCACCA TCGGAGGGAG CAGGGACACA GGACTCTACC AAGCCCCTGG 
     2461 AGGTTTTACT CCTGGAGAAG AACCGCTCGC TGCAGTCCGA GAATGCCACG CTGCGCATCT 
     2521 CCAACAGTGA CCTGAGCGGG CGCTGTGCGG AGCTGCAGAT CCACCTCACT GAGGCCACAG 
     2581 CCAAGGCTGT TGAGCAGAAG GAGCTGATCG CTCGCTTGGA GCAGGACCTC AGCACCATCC 
     2641 AGTCCATCCA ACGGCCTGAT GCCGAGGGAG CTTCCGAGCA AGGCCTAGAG AAGATTCCAG 
     2701 AACCCATCAA GGAAGCTACA GCTCTGTTCT ATGGACCCTC AATGTCATCC AGTGGGACCC 
     2761 TTCCAGAAGG CCAGGTGGAC TCCCTGCTTT CCATCATCTC CAGCCAAAGG GAACGTTTCC 
     2821 GCACCCGGAA CCAAGAGCTG GAAGCCGAGA GCCGCATGGC CCAGCACACC ATCCAGGCCC 
     2881 TGCAGAGCGA GCTGGACAGC CTGCGCGCTG ACAACATCAA ACTCTTTGAG AAGATCAAGT 
     2941 TCCTGCAGAG TTACCCTGGC AGAGGTATCG GCAGTGACGA CACGGAGCTG CGATACTCCT 
     3001 CCCAATACGA GGAACGCCTG GACCCTTTCT CCTCCTTCAG CAAGAGGGAG CGGCAGAGGA 
     3061 AGTACCTGGG CCTGAGCCCC TGGGACAAGG CCACACTTGG CATGGGCCGT CTGATTCTCT 
     3121 CCAACAAGAT GGCCCGCACC ATCAGCTTCT TTTACACCTT GTTCCTGCAC TGCCTGGTCT 
     3181 TTCTGGTGCT GTACAAGCTG GCATGGAGTG AGAGTGTGGA GAGAGACTGT GCTGCCACCT 
     3241 GCGCCAAGAA GTTCGCCGAT CATCTGCACA AATTCCACGA GAGTGACAAC GGAGCAGCAG 
     3301 CTGGTGACTT ATGGCAGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3361 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3421 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3481 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3541 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3601 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3661 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3721 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3781 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3841 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3901 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     3961 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4021 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4081 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4141 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4201 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4261 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4321 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4381 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4441 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4501 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4561 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4621 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4681 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4741 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4801 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4861 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4921 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     4981 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5041 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5101 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5161 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5221 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5281 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5341 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5401 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5461 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5521 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5581 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5641 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5701 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5761 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5821 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5881 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     5941 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6001 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6061 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6121 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6181 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6241 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6301 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6361 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6421 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6481 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6601 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6661 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6721 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6781 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6841 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6901 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     6961 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7021 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7081 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7141 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//