LOCUS       MR231082                7152 bp    DNA     circular  SYN 
DEFINITION  Mfn2 (myc-DDK-tagged) - Mouse mitofusin 2 (Mfn2), transcript variant 1
ACCESSION   NM_001285920
VERSION     NM_001285920.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3309..3400 
                     /label="Myc-DDK"  
     misc_feature    3430..4015 
                     /label="PolyA signal"  
     misc_feature    3487..3506 
                     /label="Restriction Enzyme"  
     misc_feature    4291..4963 
                     /label="ColE1"  
     misc_feature    5393..6187 
                     /label="Kan/Neo"  
     misc_feature    6222..6524 
                     /label="SV40 ori"  
     misc_feature    6610..7087 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3291..3297 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3291 
                     /label="Mfn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCTGCTC TTTTCTCGAT GCAACTCCAT CGTCACCGTC AAGAAGGATA 
     1081 AGCGACACAT GGCTGAAGTG AATGCTTCCC CTCTCAAGCA CTTTGTCACT GCCAAGAAAA 
     1141 AGATCAATGG AATCTTTGAG CAGCTGGGGG CCTACATCCA AGAGAGCGCC AGCTTCCTTG 
     1201 AAGACACCCA CAGGAACACA GAACTGGACC CGGTTACCAC GGAAGAGCAG GTCCTGGACG 
     1261 TCAAAGGGTA CCTGTCCAAG GTCAGGGGTA TCAGCGAAGT GCTGGCCAGG CGGCACATGA 
     1321 AGGTGGCTTT TTTTGGCCGG ACGAGCAATG GGAAGAGCAC CGTGATCAAT GCCATGCTCT 
     1381 GGGACAAAGT TCTGCCATCT GGGATTGGTC ATACCACCAA TTGCTTCCTG CGGGTTGGGG 
     1441 GCACAGATGG CCATGAGGCC TTCCTCCTCA CAGAGGGCTC AGAAGAGAAG AAGAGTGTCA 
     1501 AGACTGTGAA CCAACTGGCC CATGCCCTCC ATCAGGACGA GCAGTTGCAT GCAGGCAGCA 
     1561 TGGTGAGTGT GATGTGGCCC AACTCCAAGT GTCCGCTCCT GAAGGATGAC CTCGTGCTGA 
     1621 TGGACAGCCC TGGGATCGAT GTTACCACGG AGCTGGACAG CTGGATTGAT AAGTTTTGCC 
     1681 TGGATGCTGA TGTGTTTGTG CTGGTGGCCA ACTCAGAGTC CACGCTGATG CAGACGGAGA 
     1741 AGCAGTTCTT CCACAAAGTG AGTGAACGTC TCTCCCGGCC CAACATCTTC ATCCTGAACA 
     1801 ACCGCTGGGA TGCGTCTGCC TCGGAGCCTG AGTACATGGA GGAGGTGCGG CGGCAGCACA 
     1861 TGGAGCGCTG CACCAGCTTT CTGGTGGATG AGCTGGGCGT GGTGGATCGA GCTCAGGCTG 
     1921 GGGACCGGAT CTTCTTCGTG TCTGCCAAGG AGGTTCTCAG CGCCAGGGTC CAGAAAGCCC 
     1981 AGGGCATGCC AGAAGGAGGC GGCGCTCTCG CAGAAGGTTT TCAAGTGAGG ATGTTTGAGT 
     2041 TTCAGAATTT CGAGAGGCAG TTTGAGGAGT GCATTTCCCA GTCTGCAGTA AAGACCAAAT 
     2101 TTGAGCAGCA CACAGTCCGG GCCAAGCAGA TTGCAGAGGC CGTTCGTCTC ATCATGGATT 
     2161 CCCTGCACAT CGCAGCTCAG GAGCAGCGGG TTTATTGCCT AGAAATGCGG GAAGAGCGGC 
     2221 AAGACCGGCT GAGGTTTATT GACAAGCAGC TGGAGCTCCT GGCTCAAGAC TACAAGCTGC 
     2281 GAATTAAGCA GATTACGGAG GAAGTGGAAA GGCAGGTGTC CACAGCCATG GCTGAAGAGA 
     2341 TCAGGCGCCT CTCTGTGCTA GTTGACGAGT ACCAGATGGA CTTCCACCCA TCCCCAGTTG 
     2401 TCCTCAAGGT TTATAAGAAC GAGCTGCACC GCCATATAGA GGAAGGTCTG GGCCGGAACC 
     2461 TGTCTGACCG CTGCTCCACT GCCATTGCCA GTTCACTGCA GACTATGCAG CAGGACATGA 
     2521 TAGACGGCTT GAAGCCCCTT CTTCCTGTAT CTATGCGGAA TCAGATAGAC ATGCTGGTCC 
     2581 CTCGACAGTG TTTCTCCCTC AGCTATGACC TGAATTGTGA CAAGCTGTGT GCTGACTTTC 
     2641 AGGAGGACAT CGAGTTCCAC TTCTCCCTTG GATGGACTAT GCTAGTGAAC AGGTTCCTGG 
     2701 GCCCCAAGAA TAGCCGCCGG GCCTTGCTAG GCTACAGTGA TCAGGTTCAG CGTCCTCTCC 
     2761 CTCTGACACC TGCCAACCCC AGCATGCCCC CCTTGCCACA GAGCTCCCTC ACCCAGGAGG 
     2821 AGCTCATGGT CTCCATGGTT ACTGGCCTGG CCTCTCTGAC GTCTAGGACC TCCATGGGCA 
     2881 TTCTTGTGGT CGGAGGAGTG GTGTGGAAGG CAGTGGGCTG GAGACTCATC GCCCTCTCCT 
     2941 TTGGACTGTA TGGCCTCCTG TACGTCTATG AGCGACTGAC CTGGACCACC AAAGCCAAAG 
     3001 AGAGGGCCTT CAAGCGCCAG TTTGTGGAAT ACGCCAGTGA GAAGCTACAG CTCATCATCA 
     3061 GTTACACCGG CTCTAACTGC AGCCACCAAG TCCAGCAGGA ATTGTCTGGG ACATTTGCTC 
     3121 ATCTGTGCCA GCAAGTTGAC ATCACCCGAG ATAATCTGGA GCAGGAAATT GCTGCCATGA 
     3181 ACAAGAAAGT CGAGGCTCTG GATTCACTTC AGAGCAGAGC CAAACTGCTC AGGAATAAAG 
     3241 CTGGCTGGTT GGACAGCGAA CTCAACATGT TCACACACCA GTACCTGCAG CCCAGCAGAA 
     3301 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3361 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3421 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3481 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3541 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3601 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3661 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3721 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3781 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3841 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3901 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3961 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4021 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4081 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4141 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4201 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4261 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4321 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4381 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4441 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4501 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4561 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4621 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4681 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4741 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4801 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4861 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4921 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4981 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5041 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5101 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5161 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5221 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5281 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5341 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5401 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5461 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5521 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5581 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5641 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5701 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5761 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5821 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5881 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5941 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6001 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6061 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6121 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6181 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6241 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6301 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6361 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6421 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6601 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6661 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6721 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6781 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6841 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6901 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6961 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7021 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7081 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7141 AACGCGAATT TT
//